MD04G1066000.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008078
Physical Location & Seq
Reverse (-)
8814106 .. 8819506
5401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1066000.v1.1.491

Sequence Viewer

Length: 2154 bp
ATGGCCGCCCACGACCAACCGGAGACACTGTACCAAGTTCTGTTCGATCGGTGCCGGAGCCTGGAGGCAAGCCACGCCAGGCTCCGAGCTGAGCTCCACCAACTCTGCCACCAGAGGAGGAAGGAGAAGGAGAAGGAGAAGCAAGAGGAGGAGGCTGAGATGCCGACGTCAGAGTATCCGGGTTCGCTGTTCAACGTGCCCGGTTTCTTCCTCTCCGGCAGCCCGTACAAAGCCGTGTTGGATAGCATAGGCCACGCCGTCTATGTCTGCGTAGCATCCAGCGGAGAGATCAATTACTGGAATCGAGCGGCTGAGGATCTCTATGGATGGAAGGACTATGAAGTCCTTGGACAGAGAGATTCTGAGAACATCATTGCTGAGGAATACTTTGCACCTCTAAAGAGAATCATGGAAAAGTTGTGCATTGGGCTATCATGGTCAGGTCAGTTCCCTCTTAAGAAGAGGTCTGGAGAAATATTTATGGCGATGGTAACCAAAACCCCGTTGTATGAGAACAATGAGCTTGTCGGTATTATCACCGTTTCAAATGACGCGGCATTGTTTAATAGATTACAAGCAGAAAAAATGAAAACATTCAAGGATCATGCCAATGTCCAATGTAGAGGATGGCAATCAAGGCACCCACGGCCACCTATTGCACCATTGCCACAGATTGCATCGTCTGTTTCTAATCTGGCCTCAAGACTTCTATCAAGAAATGAAGACGATGTATGCAATTCAAGTGCGATGAACAAGGGCAGAGAGGACTCTACAATACAAACCGAAGATGCTGACTTAGAGAAACCTGGTAAACTGGCAGCAAAGTTCTTGTCAAAGCTGCGTACTATAGGAACCAACAAAGGTGGAAACCAAGACGATAGAAGCTCTCAACAAAGTGGTAGAACTGGTGCTTCTCTATTTAGAAATGAAGTGACTAAGGATTCTTATTCCCCAAGAAAGTCAAAAGCATCAGGTACATGCCACTGCTTTGTTGATACAGACAGTAAGGAAGAAAAACCTGGTAATGGCCATTCTTCTCATGACGATAATAGAACTCATCCAAATGCATATGGGCACAAAAACTCAATTCCTTCAGGTGAGGGTTCTGTTTTAGCCTCTTCAAGGGAGCGCAATGCGGATTTTGGGTCACCCAGACCGGGGTATCTGTTGCCAAGATTACGCTACCGAGTGGAGGGAAAGGATCCAAAACCTGAAGAACGCTACTTGAATACTTTGGACATTCAAGATTCAGTGCATGGACAAAATGGTAAACAACTACCAAATTTAGACAGCAGTGATAGTTGTTTGAGCAAAGGGGATAACAACTCTAACTCCAGAGAAGATTGTGAGATTTCGTGGGATGACTTGCATTTGGGAGATGCAATTGGACAAGGCTCATGTGCCATTGTTTATCGTGGAATCTGGAATGAATCGGATGTTGCTATTAAGGTTTACTTTAAGAATGAATACAGTGAAGGGATTTTACAAGACTACAAGAAGGAGATTGATATAATGAAAAGATTGCGACATCCTAATGTCTTGTTGTTTATGGGAGCTGTGTGCTCACAAGAACACCTTGCCATCGTCACAGAGTATTTACCTAGGGGAAGTCTCTTTAAACAACTTCACAAGAACAATCAGACATTAGACATCAGACGACGGCTGGTGATGGCTCTTGATGTTGCTAGAGGTATGAATTACTTGCATCATAGAAATCCACCTATAGTTCACAGAGATTTGAAATCGTCTAACCTGCTGGTCGATAAGAATTGGACTGTCAAGGTTGGAGACTTTGATCTGTCAAAGTTGAAGAATGCAACCTTCTTGAGTGCAAAATCTGGGAGAGGAACACCTCAGTGGATGGCCCCTGAAGTCCTTCGAAACGAACCATCTAATGAGAAGTCAGATGTGTTTAGCTTCGGTGTCATCCTGTGGGAACTAATGACTCAATCGGTCCCATGGAATAACCTCAATTCCTTACAGGTGGTTGGAATTGTAGGGTTCATGGATAGAAGATTAGACATACCAGAAGGCCTCGATCCCCAAGTAGTATCCATCATCGAAGACTGTTGGCAAAGCGGCCTGGAACAACGTCCATCATTTGAAGACATAATTCAGAGAATGAAGGGCCTAATCCAGAAAGTTGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

718

Amino Acids

80.85

Weight (kDa)

6.72

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 76 - 182 4e-07 PAS fold
PAS_9 PF13426 88 - 184 1.6e-06 PAS domain
PK_Tyr_Ser-Thr PF07714 456 - 707 1.3e-63 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 458 - 706 7.3e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AatII GACGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 1761
AccB1I GGYRCC 2 cut(s) 51, 639
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 1 cut(s) 554
AciI CCGC 6 cut(s) 6, 282, 308, 554, 1136, 2079
AclWI GGATC 5 cut(s) 324, 609, 1196, 1209, 2033
AcoI YGGCCR 3 cut(s) 3, 647, 1027
AcsI RAATTY 1 cut(s) 1282
AcuI CTGAAG 3 cut(s) 1077, 1233, 1890
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 4 cut(s) 32, 227, 844, 976
AfiI CCNNNNNNNGG 6 cut(s) 61, 1025, 1122, 1157, 1158, 1192
AflII CTTAAG 1 cut(s) 455
AjnI CCWGG 5 cut(s) 60, 77, 805, 1018, 2082
AleI CACNNNNGTG 1 cut(s) 1855
AluBI AGCT 7 cut(s) 89, 94, 523, 838, 885, 1556, 1917
AluI AGCT 7 cut(s) 89, 94, 523, 838, 885, 1556, 1917
Alw21I GWGCWC 2 cut(s) 96, 1565
Alw26I GTCTC 3 cut(s) 17, 1616, 1782
AlwI GGATC 5 cut(s) 324, 609, 1196, 1209, 2033
AoxI GGCC 9 cut(s) 3, 250, 647, 696, 1027, 1863, 2032, 2080, 2128
ApeKI GCWGC 3 cut(s) 219, 818, 838
ApoI RAATTY 1 cut(s) 1282
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 2 cut(s) 593, 1875
AspA2I CCTAGG 1 cut(s) 1601
AspLEI GCGC 1 cut(s) 1131
AspS9I GGNCC 3 cut(s) 1864, 1954, 2128
AsuC2I CCSGG 3 cut(s) 180, 201, 1158
AsuHPI GGTGA 4 cut(s) 529, 1109, 1140, 1678
AsuII TTCGAA 1 cut(s) 1879
AvaII GGWCC 1 cut(s) 1954
AvrII CCTAGG 1 cut(s) 1601
BaeGI GKGCMC 2 cut(s) 201, 1077
BaeI ACNNNNGTAYC 2 cut(s) 1145, 1178
BalI TGGCCA 1 cut(s) 1029
BamHI GGATCC 1 cut(s) 1201
BanI GGYRCC 2 cut(s) 51, 639
BanII GRGCYC 1 cut(s) 96
BarI GAAGNNNNNNTAC 2 cut(s) 1467, 1499
BbsI GAAGAC 3 cut(s) 729, 2070, 2112
Bbv12I GWGCWC 2 cut(s) 96, 1565
BbvCI CCTCAGC 2 cut(s) 312, 378
BbvI GCAGC 3 cut(s) 231, 825, 830
BccI CCATC 9 cut(s) 321, 481, 621, 1589, 1663, 1855, 1897, 2063, 2104
BceAI ACGGC 4 cut(s) 218, 242, 662, 1676
BciT130I CCWGG 5 cut(s) 62, 79, 807, 1020, 2084
BciVI GTATCC 2 cut(s) 186, 2062
BcnI CCSGG 3 cut(s) 180, 201, 1158
BcoDI GTCTC 3 cut(s) 17, 1616, 1782
BfaI CTAG 2 cut(s) 1602, 1686
BfmI CTRYAG 2 cut(s) 846, 1722
BfrI CTTAAG 1 cut(s) 455
BfuAI ACCTGC 1 cut(s) 1761
BfuI GTATCC 2 cut(s) 186, 2062
BisI GCNGC 7 cut(s) 6, 220, 309, 555, 819, 839, 2080
BlnI CCTAGG 1 cut(s) 1601
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 7 cut(s) 7, 221, 310, 556, 820, 840, 2081
Bme1390I CCNGG 8 cut(s) 62, 79, 180, 201, 807, 1020, 1158, 2084
Bme18I GGWCC 1 cut(s) 1954
BmgT120I GGNCC 3 cut(s) 1864, 1954, 2128
BmiI GGNNCC 8 cut(s) 53, 59, 83, 641, 853, 1203, 1866, 1956
BmrFI CCNGG 8 cut(s) 62, 79, 180, 201, 807, 1020, 1158, 2084
BmsI GCATC 7 cut(s) 150, 284, 686, 778, 977, 1369, 1714
BpiI GAAGAC 3 cut(s) 729, 2070, 2112
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 3 cut(s) 83, 489, 1318
Bpu10I CCTNAGC 2 cut(s) 312, 378
Bpu1102I GCTNAGC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 1879
BpuEI CTTGAG 2 cut(s) 685, 1846
BpuMI CCSGG 3 cut(s) 180, 201, 1158
BsaBI GATNNNNATC 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 5 cut(s) 346, 644, 1157, 1601, 1958
BsaWI WCCGGW 1 cut(s) 19
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 1316, 1346
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 1025, 1122, 1157, 1158, 1192
Bse1I ACTGG 3 cut(s) 302, 819, 910
Bse3DI GCAATG 3 cut(s) 372, 662, 1138
Bse8I GATNNNNATC 1 cut(s) 631
BseBI CCWGG 5 cut(s) 62, 79, 807, 1020, 2084
BseDI CCNNGG 5 cut(s) 346, 644, 1157, 1601, 1958
BseGI GGATG 9 cut(s) 275, 332, 632, 1057, 1366, 1441, 1528, 1866, 1926
BseJI GATNNNNATC 1 cut(s) 631
BseLI CCNNNNNNNGG 6 cut(s) 61, 1025, 1122, 1157, 1158, 1192
BseMI GCAATG 3 cut(s) 372, 662, 1138
BseMII CTCAG 6 cut(s) 81, 147, 303, 354, 369, 1868
BseNI ACTGG 3 cut(s) 302, 819, 910
BseRI GAGGAG 3 cut(s) 130, 161, 164
BseSI GKGCMC 2 cut(s) 201, 1077
BseXI GCAGC 3 cut(s) 231, 825, 830
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 9 cut(s) 5, 252, 649, 698, 1029, 1865, 2034, 2082, 2130
BshNI GGYRCC 2 cut(s) 51, 639
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 96, 1565
BsiSI CCGG 6 cut(s) 20, 55, 179, 201, 216, 1157
BslFI GGGAC 1 cut(s) 1940
BslI CCNNNNNNNGG 6 cut(s) 61, 1025, 1122, 1157, 1158, 1192
BsmAI GTCTC 3 cut(s) 17, 1616, 1782
BsmFI GGGAC 1 cut(s) 1940
BsmI GAATGC 1 cut(s) 1819
BsnI GGCC 9 cut(s) 5, 252, 649, 698, 1029, 1865, 2034, 2082, 2130
Bsp119I TTCGAA 1 cut(s) 1879
Bsp1286I GDGCHC 4 cut(s) 96, 201, 1077, 1565
Bsp143I GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
Bsp1720I GCTNAGC 1 cut(s) 90
Bsp19I CCATGG 1 cut(s) 1958
BspACI CCGC 6 cut(s) 6, 282, 308, 554, 1136, 2079
BspANI GGCC 9 cut(s) 5, 252, 649, 698, 1029, 1865, 2034, 2082, 2130
BspCNI CTCAG 6 cut(s) 82, 148, 304, 355, 370, 1867
BspFNI CGCG 1 cut(s) 554
BspHI TCATGA 2 cut(s) 1039, 2150
BspLI GGNNCC 8 cut(s) 53, 59, 83, 641, 853, 1203, 1866, 1956
BspMI ACCTGC 1 cut(s) 1761
BspPI GGATC 5 cut(s) 324, 609, 1196, 1209, 2033
BspT104I TTCGAA 1 cut(s) 1879
BspT107I GGYRCC 2 cut(s) 51, 639
BspTI CTTAAG 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 3 cut(s) 372, 662, 1138
BsrI ACTGG 3 cut(s) 302, 819, 910
BssECI CCNNGG 5 cut(s) 346, 644, 1157, 1601, 1958
BssMI GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
BssNI GRCGYC 1 cut(s) 167
BssT1I CCWWGG 3 cut(s) 346, 1601, 1958
Bst2UI CCWGG 5 cut(s) 62, 79, 807, 1020, 2084
Bst4CI ACNGT 6 cut(s) 30, 541, 1004, 1472, 1777, 2069
Bst6I CTCTTC 2 cut(s) 455, 1123
BstACI GRCGYC 1 cut(s) 167
BstAFI CTTAAG 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 1879
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 8 cut(s) 90, 156, 312, 363, 378, 796, 936, 1854
BstDSI CCRYGG 2 cut(s) 644, 1958
BstEII GGTNACC 2 cut(s) 490, 1146
BstENI CCTNNNNNAGG 1 cut(s) 1120
BstF5I GGATG 9 cut(s) 275, 332, 632, 1057, 1366, 1441, 1528, 1866, 1926
BstFNI CGCG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 1131
BstKTI GATC 7 cut(s) 49, 291, 319, 604, 1204, 1798, 2041
BstMAI GTCTC 3 cut(s) 17, 1616, 1782
BstMBI GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 3 cut(s) 74, 637, 646
BstNI CCWGG 5 cut(s) 62, 79, 807, 1020, 2084
BstNSI RCATGY 1 cut(s) 981
BstPI GGTNACC 2 cut(s) 490, 1146
BstSCI CCNGG 8 cut(s) 60, 77, 178, 199, 805, 1018, 1156, 2082
BstSFI CTRYAG 2 cut(s) 846, 1722
BstSLI GKGCMC 2 cut(s) 201, 1077
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 231, 825, 830
BstV2I GAAGAC 3 cut(s) 729, 2070, 2112
BstX2I RGATCY 2 cut(s) 316, 1201
BstYI RGATCY 2 cut(s) 316, 1201
BsuI GTATCC 2 cut(s) 186, 2062
BsuRI GGCC 9 cut(s) 5, 252, 649, 698, 1029, 1865, 2034, 2082, 2130
BtgI CCRYGG 2 cut(s) 644, 1958
BtgZI GCGATG 2 cut(s) 500, 761
BtsCI GGATG 9 cut(s) 275, 332, 632, 1057, 1366, 1441, 1528, 1866, 1926
BtsI GCAGTG 2 cut(s) 982, 1300
BtsIMutI CAGTG 6 cut(s) 26, 982, 1257, 1300, 1477, 1862
BveI ACCTGC 1 cut(s) 1761
Cac8I GCNNGC 1 cut(s) 70
CciI TCATGA 2 cut(s) 1039, 2150
CfoI GCGC 1 cut(s) 1131
Cfr13I GGNCC 3 cut(s) 1864, 1954, 2128
CseI GACGC 1 cut(s) 560
CsiI ACCWGGT 2 cut(s) 805, 1018
Csp6I GTAC 4 cut(s) 31, 226, 843, 975
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviQI GTAC 4 cut(s) 31, 226, 843, 975
DdeI CTNAG 8 cut(s) 90, 156, 312, 363, 378, 796, 936, 1854
DpnI GATC 7 cut(s) 48, 290, 318, 603, 1203, 1797, 2040
DpnII GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
DraI TTTAAA 1 cut(s) 1618
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 3 cut(s) 3, 647, 1027
Eam1104I CTCTTC 2 cut(s) 455, 1123
EarI CTCTTC 2 cut(s) 455, 1123
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 346, 1601, 1958
Eco147I AGGCCT 1 cut(s) 2034
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 1954
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 3 cut(s) 1077, 1233, 1890
Eco91I GGTNACC 2 cut(s) 490, 1146
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 1120
EcoO109I RGGNCCY 1 cut(s) 2128
EcoO65I GGTNACC 2 cut(s) 490, 1146
EcoRII CCWGG 5 cut(s) 60, 77, 805, 1018, 2082
EcoT14I CCWWGG 3 cut(s) 346, 1601, 1958
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 3 cut(s) 346, 1601, 1958
FalI AAGNNNNNCTT 4 cut(s) 1439, 1471, 1560, 1592
FaqI GGGAC 1 cut(s) 1940
FauNDI CATATG 1 cut(s) 1069
Fnu4HI GCNGC 7 cut(s) 6, 220, 309, 555, 819, 839, 2080
FokI GGATG 9 cut(s) 262, 339, 639, 1044, 1373, 1448, 1515, 1873, 1913
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 7 cut(s) 6, 220, 309, 555, 819, 839, 2080
FspBI CTAG 2 cut(s) 1602, 1686
GlaI GCGC 1 cut(s) 1130
GluI GCNGC 7 cut(s) 6, 220, 309, 555, 819, 839, 2080
GsuI CTGGAG 3 cut(s) 83, 489, 1318
HaeIII GGCC 9 cut(s) 5, 252, 649, 698, 1029, 1865, 2034, 2082, 2130
HapII CCGG 6 cut(s) 20, 55, 179, 201, 216, 1157
HgaI GACGC 1 cut(s) 560
HhaI GCGC 1 cut(s) 1131
Hin1I GRCGYC 1 cut(s) 167
Hin6I GCGC 1 cut(s) 1129
HinP1I GCGC 1 cut(s) 1129
HinfI GANTC 9 cut(s) 301, 359, 405, 767, 941, 1247, 1419, 1430, 1945
HpaII CCGG 6 cut(s) 20, 55, 179, 201, 216, 1157
HphI GGTGA 4 cut(s) 529, 1109, 1140, 1678
Hpy166II GTNNAC 4 cut(s) 812, 1271, 1453, 1729
Hpy188I TCNGA 8 cut(s) 86, 172, 364, 1435, 1641, 1655, 1906, 2118
Hpy8I GTNNAC 4 cut(s) 812, 1271, 1453, 1729
Hpy99I CGWCG 2 cut(s) 169, 1662
HpyCH4III ACNGT 6 cut(s) 30, 541, 1004, 1472, 1777, 2069
HpyCH4IV ACGT 3 cut(s) 167, 195, 2092
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 637, 646
HpyF3I CTNAG 8 cut(s) 90, 156, 312, 363, 378, 796, 936, 1854
HpySE526I ACGT 3 cut(s) 167, 195, 2092
Hsp92I GRCGYC 1 cut(s) 167
HspAI GCGC 1 cut(s) 1129
Kzo9I GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
LmnI GCTCC 5 cut(s) 57, 87, 99, 1126, 1553
Lsp1109I GCAGC 3 cut(s) 231, 825, 830
LweI GCATC 7 cut(s) 150, 284, 686, 778, 977, 1369, 1714
MabI ACCWGGT 2 cut(s) 805, 1018
MaeI CTAG 2 cut(s) 1602, 1686
MaeII ACGT 3 cut(s) 167, 195, 2092
MaeIII GTNAC 4 cut(s) 490, 931, 1146, 1585
MalI GATC 7 cut(s) 48, 290, 318, 603, 1203, 1797, 2040
MbiI CCGCTC 1 cut(s) 308
MboI GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
MfeI CAATTG 1 cut(s) 1383
MflI RGATCY 2 cut(s) 316, 1201
MhlI GDGCHC 4 cut(s) 96, 201, 1077, 1565
MlsI TGGCCA 1 cut(s) 1029
MluNI TGGCCA 1 cut(s) 1029
MlyI GAGTC 2 cut(s) 761, 1939
MmeI TCCRAC 3 cut(s) 219, 1765, 1969
Mox20I TGGCCA 1 cut(s) 1029
Mph1103I ATGCAT 1 cut(s) 1069
MroXI GAANNNNTTC 2 cut(s) 593, 1875
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 5 cut(s) 456, 564, 1446, 1458, 1617
MslI CAYNNNNRTG 4 cut(s) 609, 1062, 1533, 1855
Msp20I TGGCCA 1 cut(s) 1029
MspA1I CMGCKG 1 cut(s) 282
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 6 cut(s) 20, 55, 179, 201, 216, 1157
MspR9I CCNGG 8 cut(s) 62, 79, 180, 201, 807, 1020, 1158, 2084
MunI CAATTG 1 cut(s) 1383
Mva1269I GAATGC 1 cut(s) 1819
MvaI CCWGG 5 cut(s) 62, 79, 807, 1020, 2084
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 3 cut(s) 74, 637, 646
NciI CCSGG 3 cut(s) 180, 201, 1158
NcoI CCATGG 1 cut(s) 1958
NdeI CATATG 1 cut(s) 1069
NdeII GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
NlaIV GGNNCC 8 cut(s) 53, 59, 83, 641, 853, 1203, 1866, 1956
NmuCI GTSAC 3 cut(s) 931, 1146, 1585
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 1 cut(s) 981
NspV TTCGAA 1 cut(s) 1879
OliI CACNNNNGTG 1 cut(s) 1855
PagI TCATGA 2 cut(s) 1039, 2150
PceI AGGCCT 1 cut(s) 2034
PctI GAATGC 1 cut(s) 1819
PdmI GAANNNNTTC 2 cut(s) 593, 1875
PfeI GAWTC 7 cut(s) 301, 359, 405, 941, 1247, 1419, 1430
PkrI GCNGC 7 cut(s) 7, 221, 310, 556, 820, 840, 2081
Ple19I CGATCG 1 cut(s) 49
PleI GAGTC 2 cut(s) 761, 1939
PpsI GAGTC 2 cut(s) 761, 1939
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 5 cut(s) 60, 77, 805, 1018, 2082
PspEI GGTNACC 2 cut(s) 490, 1146
PspGI CCWGG 5 cut(s) 60, 77, 805, 1018, 2082
PspN4I GGNNCC 8 cut(s) 53, 59, 83, 641, 853, 1203, 1866, 1956
PspPI GGNCC 3 cut(s) 1864, 1954, 2128
PsuI RGATCY 2 cut(s) 316, 1201
PvuI CGATCG 1 cut(s) 49
RsaI GTAC 4 cut(s) 32, 227, 844, 976
RsaNI GTAC 4 cut(s) 31, 226, 843, 975
RseI CAYNNNNRTG 4 cut(s) 609, 1062, 1533, 1855
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 5 cut(s) 456, 564, 1446, 1458, 1617
SatI GCNGC 7 cut(s) 6, 220, 309, 555, 819, 839, 2080
Sau3AI GATC 7 cut(s) 46, 288, 316, 601, 1201, 1795, 2038
Sau96I GGNCC 3 cut(s) 1864, 1954, 2128
SchI GAGTC 2 cut(s) 761, 1939
ScrFI CCNGG 8 cut(s) 62, 79, 180, 201, 807, 1020, 1158, 2084
SduI GDGCHC 4 cut(s) 96, 201, 1077, 1565
SexAI ACCWGGT 2 cut(s) 805, 1018
SfaNI GCATC 7 cut(s) 150, 284, 686, 778, 977, 1369, 1714
SfcI CTRYAG 2 cut(s) 846, 1722
SfuI TTCGAA 1 cut(s) 1879
SinI GGWCC 1 cut(s) 1954
SmiMI CAYNNNNRTG 4 cut(s) 609, 1062, 1533, 1855
SmlI CTYRAG 3 cut(s) 455, 700, 1825
SmoI CTYRAG 3 cut(s) 455, 700, 1825
SseBI AGGCCT 1 cut(s) 2034
SsiI CCGC 6 cut(s) 6, 282, 308, 554, 1136, 2079
SspI AATATT 1 cut(s) 477
SspMI CTAG 2 cut(s) 1602, 1686
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 2034
StyD4I CCNGG 8 cut(s) 60, 77, 178, 199, 805, 1018, 1156, 2082
StyI CCWWGG 3 cut(s) 346, 1601, 1958
TaaI ACNGT 6 cut(s) 30, 541, 1004, 1472, 1777, 2069
TaiI ACGT 3 cut(s) 170, 198, 2095
TaqI TCGA 6 cut(s) 45, 304, 1761, 1879, 2037, 2061
TaqII GACCGA 1 cut(s) 1942
TauI GCSGC 4 cut(s) 8, 311, 557, 2082
TfiI GAWTC 7 cut(s) 301, 359, 405, 941, 1247, 1419, 1430
Tru1I TTAA 5 cut(s) 456, 564, 1446, 1458, 1617
Tru9I TTAA 5 cut(s) 456, 564, 1446, 1458, 1617
TscAI CASTG 6 cut(s) 33, 989, 1257, 1300, 1477, 1862
TseFI GTSAC 3 cut(s) 931, 1146, 1585
TseI GCWGC 3 cut(s) 219, 818, 838
Tsp45I GTSAC 3 cut(s) 931, 1146, 1585
TspRI CASTG 6 cut(s) 33, 989, 1257, 1300, 1477, 1862
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 1954
XagI CCTNNNNNAGG 1 cut(s) 1120
XapI RAATTY 1 cut(s) 1282
XceI RCATGY 1 cut(s) 981
XmaJI CCTAGG 1 cut(s) 1601
XmnI GAANNNNTTC 2 cut(s) 593, 1875
XspI CTAG 2 cut(s) 1602, 1686
ZraI GACGTC 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.