FvH4_5g01850

GTPase activator activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009686
Physical Location & Seq
Forward (+)
1160312 .. 1161357
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01850.t1

Sequence Viewer

Length: 381 bp
ATGGCTCGAGGTACCCTCGACGTTAGAATTTGTGGTGGAAGAAATCTCAGTAACCCGAACAAAGAAGGTAAGATTTGTTGCATCGTCTACTACAAAGATCAGCAGAAGAGGACAGAAGCCATATACTGTGATGGCCCCGATCCAGAGTGGAACGAGACACTTCTGTTCACCATAGCTGATCATGTTGAAGAGCTCAAGGTCAGGGTCCATGACGGGGATTATGTTACTGAAGAAGAAGTTGGACACTGCGATATTAATATAGAGGGTCTACTTCGGGACGGGGGAGAAAATCGAATAGAGCCGACTTCATATGATGTATGGAGGAATAATGAAGGAAACTATGGAAATGTTACTGTTGAACTCCATTTTCATCCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 C2 EF-hand_8

Protein Analysis

127

Amino Acids

14.44

Weight (kDa)

4.74

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 4 - 96 2.5e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 11
AccB1I GGYRCC 1 cut(s) 11
AccI GTMKAC 2 cut(s) 87, 268
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 2 cut(s) 188, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 2 cut(s) 176, 193
AluI AGCT 2 cut(s) 176, 193
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 27
AseI ATTAAT 1 cut(s) 255
Asp718I GGTACC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 134, 205
AsuHPI GGTGA 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BccI CCATC 1 cut(s) 125
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 2 cut(s) 134, 205
BmiI GGNNCC 3 cut(s) 13, 136, 206
BmsI GCATC 1 cut(s) 90
BplI GAGNNNNNCTC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 61
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 3 cut(s) 97, 139, 178
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 13, 136, 206
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 11
BssMI GATC 3 cut(s) 97, 139, 178
Bst4CI ACNGT 2 cut(s) 128, 355
Bst6I CTCTTC 2 cut(s) 101, 183
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 370
BstKTI GATC 3 cut(s) 100, 142, 181
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 3 cut(s) 97, 139, 178
BsuRI GGCC 1 cut(s) 135
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 134, 205
Csp6I GTAC 1 cut(s) 12
CviAII CATG 2 cut(s) 182, 209
CviJI RGCY 6 cut(s) 5, 119, 135, 176, 193, 301
CviKI_1 RGCY 6 cut(s) 5, 119, 135, 176, 193, 301
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 1 cut(s) 47
DpnI GATC 3 cut(s) 99, 141, 180
DpnII GATC 3 cut(s) 97, 139, 178
Eam1104I CTCTTC 2 cut(s) 101, 183
EarI CTCTTC 2 cut(s) 101, 183
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 205
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 2 cut(s) 185, 212
FaqI GGGAC 1 cut(s) 290
FatI CATG 2 cut(s) 181, 208
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 2 cut(s) 87, 268
FokI GGATG 1 cut(s) 357
FriOI GRGCYC 1 cut(s) 195
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 2 cut(s) 185, 212
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 88, 168, 269
Hpy188III TCNNGA 3 cut(s) 143, 275, 374
Hpy8I GTNNAC 3 cut(s) 88, 168, 269
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 59, 326
HpyCH4III ACNGT 2 cut(s) 128, 355
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 81
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 185, 212
KpnI GGTACC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 3 cut(s) 97, 139, 178
LguI GCTCTTC 1 cut(s) 183
LpnPI CCDG 2 cut(s) 156, 187
LweI GCATC 1 cut(s) 90
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 50, 223, 349
MalI GATC 3 cut(s) 99, 141, 180
MboI GATC 3 cut(s) 97, 139, 178
MboII GAAGA 5 cut(s) 51, 118, 200, 242, 245
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 1 cut(s) 27
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 4 cut(s) 26, 102, 256, 315
MseI TTAA 1 cut(s) 255
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 97, 139, 178
NlaIII CATG 2 cut(s) 185, 212
NlaIV GGNNCC 3 cut(s) 13, 136, 206
PaeR7I CTCGAG 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 195
PspN4I GGNNCC 3 cut(s) 13, 136, 206
PspPI GGNCC 2 cut(s) 134, 205
PspXI VCTCGAGB 1 cut(s) 6
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 1 cut(s) 255
Sau3AI GATC 3 cut(s) 97, 139, 178
Sau96I GGNCC 2 cut(s) 134, 205
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 13, 24, 70, 178, 195, 201
SfaNI GCATC 1 cut(s) 90
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 6, 194
SmoI CTYRAG 2 cut(s) 6, 194
Sse9I AATT 1 cut(s) 27
SstI GAGCTC 1 cut(s) 195
TaaI ACNGT 2 cut(s) 128, 355
TaiI ACGT 1 cut(s) 24
TaqI TCGA 3 cut(s) 7, 18, 292
TasI AATT 1 cut(s) 27
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 251
TspDTI ATGAA 3 cut(s) 297, 345, 359
TspRI CASTG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 205
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 1 cut(s) 27
XhoI CTCGAG 1 cut(s) 6
XmiI GTMKAC 2 cut(s) 87, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.