Rroxscaffold_3G00257530

GTPase activator activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009686
Physical Location & Seq
Forward (+)
51882227 .. 51884555
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257530.1

Sequence Viewer

Length: 420 bp
ATGGCGCCTCGAGGGGTACTTGACGTTTCAGTTATTGGTGGAAGGAATCTCTCAAACCCGGACAAAGAGGGTAAAATAATGTGCATCGTCAACTACAAAAATCAAGAGCATAGGACGGACGCCATATATTGTGATGGGACTGATCCACAGTGGAATGAGCAACTTCTGTTCACCATAGCTGGTTCAGTTGAAGATCTCAAGATTCGGGTCCACGACGGGGATTACGTTACTGATGAAGAAGTTGGACATTGCTCTATTCCAATAGACAGTCTAGTTGAGACAGGGTACAAAGCGCAAACAGACGCGTCTCCATATGAAGTATGGAGGGGAGATGAAGTCTATGGAGAACTTGCCGTTGCCCTCTCTTTTCATCCAGAGGAGGGCCATGATGATGAAGAACATGAAGAAGAGGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 C2 EF-hand_8

Protein Analysis

139

Amino Acids

15.59

Weight (kDa)

4.28

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 94 1.3e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 305
AclWI GGATC 1 cut(s) 137
AcyI GRCGYC 2 cut(s) 5, 120
AfaI GTAC 2 cut(s) 18, 287
AfiI CCNNNNNNNGG 2 cut(s) 217, 380
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 1 cut(s) 191
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw26I GTCTC 2 cut(s) 272, 312
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 1 cut(s) 382
AspLEI GCGC 2 cut(s) 7, 295
AspS9I GGNCC 2 cut(s) 208, 382
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 4
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 2 cut(s) 272, 312
BfaI CTAG 1 cut(s) 272
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 2 cut(s) 208, 382
BmiI GGNNCC 2 cut(s) 6, 209
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 59
BsaHI GRCGYC 2 cut(s) 5, 120
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 380
Bse3DI GCAATG 1 cut(s) 247
BseGI GGATG 2 cut(s) 370, 418
BseLI CCNNNNNNNGG 2 cut(s) 217, 380
BseMI GCAATG 1 cut(s) 247
BseRI GAGGAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 217, 380
BsmAI GTCTC 2 cut(s) 272, 312
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 151
BsnI GGCC 1 cut(s) 384
BsoBI CYCGRG 1 cut(s) 9
Bsp143I GATC 2 cut(s) 142, 193
BspANI GGCC 1 cut(s) 384
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 2 cut(s) 6, 209
BspPI GGATC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 247
BssMI GATC 2 cut(s) 142, 193
BssNI GRCGYC 2 cut(s) 5, 120
Bst4CI ACNGT 2 cut(s) 150, 269
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 2 cut(s) 5, 120
BstF5I GGATG 2 cut(s) 370, 418
BstFNI CGCG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 295
BstKTI GATC 2 cut(s) 145, 196
BstMAI GTCTC 2 cut(s) 272, 312
BstMBI GATC 2 cut(s) 142, 193
BstSCI CCNGG 1 cut(s) 57
BstUI CGCG 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 2 cut(s) 370, 418
BtsIMutI CAGTG 1 cut(s) 155
CfoI GCGC 2 cut(s) 7, 295
Cfr13I GGNCC 2 cut(s) 208, 382
CseI GACGC 3 cut(s) 128, 294, 311
Csp6I GTAC 2 cut(s) 17, 286
CviAII CATG 2 cut(s) 386, 401
CviJI RGCY 2 cut(s) 179, 384
CviKI_1 RGCY 2 cut(s) 179, 384
CviQI GTAC 2 cut(s) 17, 286
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 144, 195
DpnII GATC 2 cut(s) 142, 193
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 9
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 2 cut(s) 389, 404
FaqI GGGAC 1 cut(s) 151
FatI CATG 2 cut(s) 385, 400
FauNDI CATATG 1 cut(s) 313
FokI GGATG 1 cut(s) 357
FspBI CTAG 1 cut(s) 272
GlaI GCGC 2 cut(s) 6, 294
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 59
HgaI GACGC 3 cut(s) 128, 294, 311
HhaI GCGC 2 cut(s) 7, 295
Hin1I GRCGYC 2 cut(s) 5, 120
Hin1II CATG 2 cut(s) 389, 404
Hin6I GCGC 2 cut(s) 5, 293
HinP1I GCGC 2 cut(s) 5, 293
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 2 cut(s) 46, 202
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 3 cut(s) 91, 171, 211
Hpy188III TCNNGA 3 cut(s) 104, 199, 374
Hpy8I GTNNAC 3 cut(s) 91, 171, 211
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 2 cut(s) 150, 269
HpyCH4IV ACGT 2 cut(s) 24, 225
HpyCH4V TGCA 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 24, 225
Hsp92I GRCGYC 2 cut(s) 5, 120
Hsp92II CATG 2 cut(s) 389, 404
HspAI GCGC 2 cut(s) 5, 293
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 142, 193
LpnPI CCDG 4 cut(s) 72, 165, 267, 387
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 272
MaeII ACGT 2 cut(s) 24, 225
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 144, 195
MboI GATC 2 cut(s) 142, 193
MboII GAAGA 5 cut(s) 203, 248, 407, 416, 419
MflI RGATCY 1 cut(s) 193
MluI ACGCGT 1 cut(s) 303
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 8 cut(s) 5, 18, 61, 318, 370, 371, 373, 403
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 305
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 313
NdeII GATC 2 cut(s) 142, 193
NlaIII CATG 2 cut(s) 389, 404
NlaIV GGNNCC 2 cut(s) 6, 209
PaeR7I CTCGAG 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 2 cut(s) 46, 202
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 6, 209
PspPI GGNCC 2 cut(s) 208, 382
PspXI VCTCGAGB 1 cut(s) 9
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 2 cut(s) 18, 287
RsaNI GTAC 2 cut(s) 17, 286
RseI CAYNNNNRTG 1 cut(s) 390
Sau3AI GATC 2 cut(s) 142, 193
Sau96I GGNCC 2 cut(s) 208, 382
ScrFI CCNGG 1 cut(s) 59
SetI ASST 3 cut(s) 27, 181, 228
SfaNI GCATC 1 cut(s) 93
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 208
SlaI CTCGAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 2 cut(s) 9, 197
SmoI CTYRAG 2 cut(s) 9, 197
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 2 cut(s) 150, 269
TaiI ACGT 2 cut(s) 27, 228
TaqI TCGA 1 cut(s) 10
TfiI GAWTC 2 cut(s) 46, 202
TscAI CASTG 1 cut(s) 155
TspDTI ATGAA 6 cut(s) 249, 330, 348, 359, 408, 417
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 208
XcmI CCANNNNNNNNNTGG 1 cut(s) 318
XhoI CTCGAG 1 cut(s) 9
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.