Rorug07G0049200

GTPase activator activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009686
Physical Location & Seq
Reverse (-)
3538200 .. 3547763
9564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0049200.1

Sequence Viewer

Length: 3129 bp
ATGGGGAGCGAGAGCAATTTGAAGACGTGGGTTTCCGATAAGCTGATGACATTGCTCGGTTATTCTCAGCCGGCTGTTGTTCAGTACATTATTGGATTAACTAAACAAGCAAAGTCTCCAGCTGATGTGGTGGAGAAGCTTTTAGATTCGGGGTGGTCCTCCTCGTCTGATACACGTAAATTTGCTGAAGAAATATTCTCCAGAGTGCCCCACAAATCATCCGGACCGAATGTTTATCAGAAACAAGAGAGGGAGGCTGCAATGCTGGTGAGAAAGCAGAAGACATATGCTCTATTGGATGCAGATGATGACGATGATGATGGTGATAAGTCTTCTGTTCCAGTAGTTTCCGAGTCCAGGAAAAGTGATTCCCATAAGAAACGGTTCAGGAGGAAGGCCTCGAGTCAGGACGATGAAGATGATGAGGTGATTGTACACCAGGAAGAAGTGAGGCGGGTTAAAAGACGAACTTCCCCAGATGAAGATGATGGTTCAGAGTCCGAAGAAGAAAGATTGCGTGATCAAAGAGAGCGGGAGCAATTAGAGCGCAATATAAGGGAGAGGGACACAGCAGCTACACGAAAGTTAACAGAGAGAAAGTTATCAAAAAAAGAGGAAGAGGAGGCTATTCGGAGAAACAAGGCTTCAGAGCGAAATGAAACTGAAGATTTAAGACTAGTTTCAAGACAAGAATATTTAAAGAAAAGACAGCAGAAGAAACTGGAGGAAATCAGAGATGAAATAGAAGATGAGCAATACTTATTTGAGCACGTGAAGCTCACTGAATTGGAACGTCGTGAATTAAGTTACAAGAAGCAAATACTTGAGGCTGTAACGAAGCGGGCAGTTGAGGACGAGAATCAAAATGAGTACAGGATTCCTGATGCCTATGATGTGGAGGGGGGTGTCAATCAGGAGAAGAGATTTTCTGTGGCCATGCAACGCTACAGGGACCTGGCTGGGGATAAAATGAATCCATTTGCAGAACAGGAAGCTTGGGAGGATCACCAAATTGGAAAGGCAACACTGCAGTATGGTTCAAAAAATAAAAAGCGATCTGATGAGTATCAATATGTATTTGAGGACCAGATTGATTTCATCAAGGCATCAGTAATGGATGGTGACCAGTTTGAGGATGGGCAACCGGCGGATTTACTTGAGTCGAGAGCGAAATCAGAGTTGGAGAAGCTCCAGGAGGACAGAAAAACATTACCCATATACTCCTATCGTGATGAGTTGCTCAAAGCTGTTGATAACCATCAGGTTCTTGTTATTGTTGGTGAAACCGGTTCTGGGAAAACTACGCAAATACCCCAGTATCTTCACGAGGCCGGATACACAAAACGTGGAAAGATTGGGTGCACACAGCCACGACGAGTTGCTGCTATGAGTGTTGCTGCAAGGGTTTCTCAAGAAATGGGTGTGAAACTTGGGCATGAGGTAGGTTATTCCATTCGTTTCGAGGATTGCACATCGGAAAAGACGGTTTTGAAATATATGACAGATGGGATGTTGTTGCGAGAATTCCTTGGTGAACCAGATCTGGCAGGCTACAGTGTGGTTATGGTGGATGAGGCCCATGAGAGAACACTTTCAACTGATATTCTATTTGGATTAGTAAAGGATATTGCTCGATTTCGACCTGATCTTAAATTACTTATCTCAAGTGCAACACTTGATGCTGAGAAGTTCAGTGATTATTTTGATTCTGCTCCAATTTTCAAAATTCCGGGGAGGCGTTATCCTGTTGAAATAAATTACACTAAAGCACCAGAAGCTGATTACTTGGATGCAGCAATTGTTACTGCACTGCAAATCCATGTGACAGAAGCATCTGGAGATATATTAGTGTTCCTGACAGGTCAAGAAGAAATTGAAACAGCGGAAGAAATATTGAAGCACAGAACAAGGGGCTTAGGGACAAAAATTGCCGAGCTTATTATTTGTCCCATATATGCAAACCTACCTACTGAGCTACAATCCAAAATTTTTGAGCCCACACCTGAAGGGGCAAGGAAAGTTGTCCTTGCGACAAATATAGCTGAAACTTCACTAACTATTGATGGGATCAAATATGTTATTGACCCAGGGTTTTGCAAGATGAAGTCATATAATCCAAGGACTGGGATGGAGTCGTTGCTGGTCGCTCCCATCTCAAAGGCATCAGCCAATCAGAGGGCAGGTCGATCTGGGAGAACGGGTCCTGGAAAGTGCTACCGGCTATATACTATGTTCAATTACCAAACTGAGTTAGAAGATAACACGGTACCAGAAATTCAGAGGACTAACCTTGCAAATGTTGTGCTCATGCTGAAAAGCCTTGGTATCCATGACCTATTACATTTTGATTTTATGGACCCGCCACCATCAGAGGCATTACTGAAAGCCTTGGAACTGTTATTTGCACTAAGTGCATTAAATAAAGTGGGTGAGTTGACTAAAGTTGGTAGACGGATGGCCGAGTTTCCTCTTGATCCTATGCTATCTAAGATGATAGTTGCTTCTGATAAGTACAAGTGTTCAGATGAGATCATTTCCATTGCTTCCATGCTTTCCATTGGTAATTCAATATTTTACCGTCCAAAGGACAAACAAGTCCATGCTGACAATGCACGGTTGAATTTTCACACTGGGAATGTGGGAGATCACATCGCATTGCTCAAGGTTTTTAATTCATGGAAAGAGACCAATTTCTCAACACAATGGTGTTACGAGAATTATATACAGGTCAGGAGTATGAAGCGCGCTAGAGATATTAGAGACCAGCTCGAGGGACTCTTGGAGAGGGTCGAGATTGAGCAGGTCTCAAATCCTGATTTTGAAGTTATAAAGAAGGCCATTACATCTGGCTTTTTCCCTCATTCCGCAAGACTGCAAAAGAGTGGATCTTATAGAACAGTCAAACATCCGCAGACTGTCCACATACACCCCAGCTCAGGGCTTTCTCAGGTGCTTCCAAGATGGGTTATATACCATGAGTTGGTGCTTACAACCAAGGAATATATGAGACAGGTAACGGAACTGAAGCCAGAGTGGTTGGTGGAAATAGCTCCGCATTATTACCAGCTAAAGGATGTTGAAGATTCGGTGTCAAAGAAAATGCCTCGTGGAGAAGGGCGCGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 C2 EF-hand_8

Protein Analysis

1042

Amino Acids

119.15

Weight (kDa)

5.83

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 608 - 734 1.1e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 796 - 824 1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 825 - 886 4.7e-11 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 943 - 1020 3e-24 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2828
AasI GACNNNNNNGTC 1 cut(s) 2594
Acc36I ACCTGC 2 cut(s) 2171, 2791
Acc65I GGTACC 1 cut(s) 2266
AccB1I GGYRCC 1 cut(s) 2266
AccB7I CCANNNNNTGG 2 cut(s) 2123, 2980
AccBSI CCGCTC 1 cut(s) 532
AccI GTMKAC 1 cut(s) 2449
AccII CGCG 2 cut(s) 2745, 3120
AccIII TCCGGA 1 cut(s) 221
AciI CCGC 9 cut(s) 454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
AclWI GGATC 4 cut(s) 1011, 2075, 2468, 2893
AcoI YGGCCR 2 cut(s) 933, 2457
AcsI RAATTY 6 cut(s) 179, 1523, 1725, 1986, 2274, 2620
AcuI CTGAAG 5 cut(s) 207, 630, 684, 2025, 3044
AcvI CACGTG 1 cut(s) 772
AdeI CACNNNGTG 1 cut(s) 2411
AfaI GTAC 5 cut(s) 86, 435, 872, 2268, 2513
AflIII ACRYGT 1 cut(s) 173
AgeI ACCGGT 1 cut(s) 1286
AhlI ACTAGT 1 cut(s) 676
AjiI CACGTC 1 cut(s) 27
AjnI CCWGG 6 cut(s) 356, 438, 954, 1191, 2086, 2203
AjuI GAANNNNNNNTTGG 2 cut(s) 1163, 1195
AleI CACNNNNGTG 1 cut(s) 2704
Alw21I GWGCWC 3 cut(s) 771, 1364, 2307
Alw26I GTCTC 5 cut(s) 120, 2678, 2754, 2809, 3001
Alw44I GTGCAC 1 cut(s) 1360
AlwI GGATC 4 cut(s) 1011, 2075, 2468, 2893
AlwNI CAGNNNCTG 1 cut(s) 1778
Ama87I CYCGRG 2 cut(s) 400, 2768
Aor13HI TCCGGA 1 cut(s) 221
AoxI GGCC 6 cut(s) 396, 933, 1329, 1575, 2457, 2835
ApaLI GTGCAC 1 cut(s) 1360
ApeKI GCWGC 5 cut(s) 257, 572, 1382, 1397, 1793
ApoI RAATTY 6 cut(s) 179, 1523, 1725, 1986, 2274, 2620
AsiGI ACCGGT 1 cut(s) 1286
Asp700I GAANNNNTTC 2 cut(s) 383, 1591
Asp718I GGTACC 1 cut(s) 2266
AspLEI GCGC 5 cut(s) 549, 2745, 2747, 3120, 3122
AspS9I GGNCC 7 cut(s) 156, 224, 952, 1084, 1576, 2201, 2356
AsuC2I CCSGG 1 cut(s) 1731
AsuHPI GGTGA 8 cut(s) 280, 335, 439, 998, 1133, 1292, 1544, 2441
AvaI CYCGRG 2 cut(s) 400, 2768
AvaII GGWCC 6 cut(s) 156, 224, 952, 1084, 2201, 2356
BaeGI GKGCMC 2 cut(s) 210, 1364
BaeI ACNNNNGTAYC 2 cut(s) 1301, 1334
BalI TGGCCA 1 cut(s) 935
BanI GGYRCC 1 cut(s) 2266
BanII GRGCYC 1 cut(s) 1998
BauI CACGAG 2 cut(s) 1325, 3105
BbrPI CACGTG 1 cut(s) 772
BbsI GAAGAC 3 cut(s) 29, 287, 324
Bbv12I GWGCWC 3 cut(s) 771, 1364, 2307
BbvI GCAGC 5 cut(s) 244, 584, 1369, 1384, 1805
BciT130I CCWGG 6 cut(s) 358, 440, 956, 1193, 2088, 2205
BciVI GTATCC 2 cut(s) 1328, 2336
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 1731
BcoDI GTCTC 5 cut(s) 120, 2678, 2754, 2809, 3001
BcuI ACTAGT 1 cut(s) 676
BfaI CTAG 2 cut(s) 677, 2748
BfmI CTRYAG 3 cut(s) 946, 1028, 1552
BfuAI ACCTGC 2 cut(s) 2171, 2791
BfuI GTATCC 2 cut(s) 1328, 2336
BglII AGATCT 1 cut(s) 1540
BisI GCNGC 5 cut(s) 258, 573, 1383, 1398, 1794
BlsI GCNGC 5 cut(s) 259, 574, 1384, 1399, 1795
Bme1390I CCNGG 7 cut(s) 358, 440, 956, 1193, 1731, 2088, 2205
Bme18I GGWCC 6 cut(s) 156, 224, 952, 1084, 2201, 2356
BmeT110I CYCGRG 2 cut(s) 400, 2768
BmgBI CACGTC 1 cut(s) 27
BmgT120I GGNCC 7 cut(s) 156, 224, 952, 1084, 1576, 2201, 2356
BmiI GGNNCC 4 cut(s) 953, 2202, 2268, 2358
BmrFI CCNGG 7 cut(s) 358, 440, 956, 1193, 1731, 2088, 2205
BmrI ACTGGG 3 cut(s) 1309, 2133, 2640
BmsI GCATC 7 cut(s) 289, 874, 1115, 1669, 1780, 1841, 2171
BmuI ACTGGG 3 cut(s) 1309, 2133, 2640
BpiI GAAGAC 3 cut(s) 29, 287, 324
BplI GAGNNNNNCTC 4 cut(s) 2751, 2783, 2789, 2821
BpmI CTGGAG 5 cut(s) 102, 184, 743, 1175, 1857
Bpu10I CCTNAGC 2 cut(s) 1915, 2935
BpuEI CTTGAG 5 cut(s) 845, 1178, 1395, 1648, 2645
BpuMI CCSGG 1 cut(s) 1731
BsaAI YACGTR 2 cut(s) 176, 772
BsaBI GATNNNNATC 2 cut(s) 1065, 1257
BsaI GGTCTC 3 cut(s) 2678, 2754, 2809
BsaJI CCNNGG 8 cut(s) 1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BsaWI WCCGGW 2 cut(s) 221, 1286
BsaXI ACNNNNNCTCC 2 cut(s) 2185, 2215
Bse118I RCCGGY 4 cut(s) 70, 1144, 1286, 2217
Bse1I ACTGG 6 cut(s) 341, 726, 1126, 1315, 2128, 2635
Bse3DI GCAATG 4 cut(s) 50, 267, 2538, 2654
Bse8I GATNNNNATC 2 cut(s) 1065, 1257
BseAI TCCGGA 1 cut(s) 221
BseBI CCWGG 6 cut(s) 358, 440, 956, 1193, 2088, 2205
BseDI CCNNGG 8 cut(s) 1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BseJI GATNNNNATC 2 cut(s) 1065, 1257
BseMI GCAATG 4 cut(s) 50, 267, 2538, 2654
BseMII CTCAG 6 cut(s) 80, 1674, 1962, 2238, 2949, 2960
BseNI ACTGG 6 cut(s) 341, 726, 1126, 1315, 2128, 2635
BsePI GCGCGC 2 cut(s) 2743, 3118
BseRI GAGGAG 2 cut(s) 151, 635
BseSI GKGCMC 2 cut(s) 210, 1364
BseXI GCAGC 5 cut(s) 244, 584, 1369, 1384, 1805
BseYI CCCAGC 2 cut(s) 959, 2930
BsgI GTGCAG 1 cut(s) 1791
Bsh1236I CGCG 2 cut(s) 2745, 3120
BshFI GGCC 6 cut(s) 398, 935, 1331, 1577, 2459, 2837
BshNI GGYRCC 1 cut(s) 2266
BshTI ACCGGT 1 cut(s) 1286
BsiHKAI GWGCWC 3 cut(s) 771, 1364, 2307
BsiHKCI CYCGRG 2 cut(s) 400, 2768
BsiSI CCGG 7 cut(s) 71, 222, 1145, 1287, 1332, 1730, 2218
BslFI GGGAC 5 cut(s) 578, 965, 1932, 1933, 2787
BsmAI GTCTC 5 cut(s) 120, 2678, 2754, 2809, 3001
BsmFI GGGAC 5 cut(s) 578, 965, 1932, 1933, 2787
BsnI GGCC 6 cut(s) 398, 935, 1331, 1577, 2459, 2837
Bso31I GGTCTC 3 cut(s) 2678, 2754, 2809
BsoBI CYCGRG 2 cut(s) 400, 2768
Bsp1286I GDGCHC 5 cut(s) 210, 771, 1364, 1998, 2307
Bsp13I TCCGGA 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 433
BspACI CCGC 9 cut(s) 454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
BspANI GGCC 6 cut(s) 398, 935, 1331, 1577, 2459, 2837
BspCNI CTCAG 6 cut(s) 79, 1675, 1963, 2239, 2948, 2959
BspEI TCCGGA 1 cut(s) 221
BspFNI CGCG 2 cut(s) 2745, 3120
BspLI GGNNCC 4 cut(s) 953, 2202, 2268, 2358
BspMAI CTGCAG 1 cut(s) 1032
BspMI ACCTGC 2 cut(s) 2171, 2791
BspPI GGATC 4 cut(s) 1011, 2075, 2468, 2893
BspT107I GGYRCC 1 cut(s) 2266
BspTNI GGTCTC 3 cut(s) 2678, 2754, 2809
BsrBI CCGCTC 1 cut(s) 532
BsrDI GCAATG 4 cut(s) 50, 267, 2538, 2654
BsrFI RCCGGY 4 cut(s) 70, 1144, 1286, 2217
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 6 cut(s) 341, 726, 1126, 1315, 2128, 2635
BssAI RCCGGY 4 cut(s) 70, 1144, 1286, 2217
BssECI CCNNGG 8 cut(s) 1528, 1730, 2086, 2087, 2117, 2320, 2388, 2994
BssHII GCGCGC 2 cut(s) 2743, 3118
BssSI CACGAG 2 cut(s) 1325, 3105
BssT1I CCWWGG 5 cut(s) 1528, 2117, 2320, 2388, 2994
Bst2BI CACGAG 2 cut(s) 1325, 3105
Bst2UI CCWGG 6 cut(s) 358, 440, 956, 1193, 2088, 2205
Bst4CI ACNGT 9 cut(s) 384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
Bst6I CTCTTC 2 cut(s) 612, 914
BstAPI GCANNNNNTGC 1 cut(s) 2411
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 2 cut(s) 176, 772
BstC8I GCNNGC 5 cut(s) 72, 843, 1549, 2745, 3120
BstDEI CTNAG 9 cut(s) 66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
BstEII GGTNACC 1 cut(s) 1121
BstFNI CGCG 2 cut(s) 2745, 3120
BstHHI GCGC 5 cut(s) 549, 2745, 2747, 3120, 3122
BstMAI GTCTC 5 cut(s) 120, 2678, 2754, 2809, 3001
BstMWI GCNNNNNNNGC 5 cut(s) 544, 775, 1775, 2411, 2609
BstNI CCWGG 6 cut(s) 358, 440, 956, 1193, 2088, 2205
BstPI GGTNACC 1 cut(s) 1121
BstSCI CCNGG 7 cut(s) 356, 438, 954, 1191, 1729, 2086, 2203
BstSFI CTRYAG 3 cut(s) 946, 1028, 1552
BstSLI GKGCMC 2 cut(s) 210, 1364
BstUI CGCG 2 cut(s) 2745, 3120
BstV1I GCAGC 5 cut(s) 244, 584, 1369, 1384, 1805
BstV2I GAAGAC 3 cut(s) 29, 287, 324
BstX2I RGATCY 2 cut(s) 1540, 2885
BstYI RGATCY 2 cut(s) 1540, 2885
BsuI GTATCC 2 cut(s) 1328, 2336
BsuRI GGCC 6 cut(s) 398, 935, 1331, 1577, 2459, 2837
BtgZI GCGATG 1 cut(s) 2635
BtrI CACGTC 1 cut(s) 27
BtsI GCAGTG 2 cut(s) 1025, 1808
BtsIMutI CAGTG 6 cut(s) 780, 1025, 1561, 1699, 1808, 2628
BveI ACCTGC 2 cut(s) 2171, 2791
Cac8I GCNNGC 5 cut(s) 72, 843, 1549, 2745, 3120
CaiI CAGNNNCTG 1 cut(s) 1778
CfoI GCGC 5 cut(s) 549, 2745, 2747, 3120, 3122
Cfr10I RCCGGY 4 cut(s) 70, 1144, 1286, 2217
Cfr13I GGNCC 7 cut(s) 156, 224, 952, 1084, 1576, 2201, 2356
CpoI CGGWCCG 1 cut(s) 224
Csp6I GTAC 5 cut(s) 85, 434, 871, 2267, 2512
CspAI ACCGGT 1 cut(s) 1286
CspI CGGWCCG 1 cut(s) 224
CviQI GTAC 5 cut(s) 85, 434, 871, 2267, 2512
DdeI CTNAG 9 cut(s) 66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
DraI TTTAAA 1 cut(s) 699
DraIII CACNNNGTG 1 cut(s) 2411
DrdI GACNNNNNNGTC 1 cut(s) 2594
DseDI GACNNNNNNGTC 1 cut(s) 2594
EaeI YGGCCR 2 cut(s) 933, 2457
Eam1104I CTCTTC 2 cut(s) 612, 914
EarI CTCTTC 2 cut(s) 612, 914
EciI GGCGGA 1 cut(s) 1163
Eco130I CCWWGG 5 cut(s) 1528, 2117, 2320, 2388, 2994
Eco147I AGGCCT 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 1998
Eco31I GGTCTC 3 cut(s) 2678, 2754, 2809
Eco47I GGWCC 6 cut(s) 156, 224, 952, 1084, 2201, 2356
Eco57I CTGAAG 5 cut(s) 207, 630, 684, 2025, 3044
Eco72I CACGTG 1 cut(s) 772
Eco88I CYCGRG 2 cut(s) 400, 2768
Eco91I GGTNACC 1 cut(s) 1121
EcoO109I RGGNCCY 2 cut(s) 952, 2201
EcoO65I GGTNACC 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 1523
EcoRII CCWGG 6 cut(s) 356, 438, 954, 1191, 2086, 2203
EcoT14I CCWWGG 5 cut(s) 1528, 2117, 2320, 2388, 2994
EcoT38I GRGCYC 1 cut(s) 1998
ErhI CCWWGG 5 cut(s) 1528, 2117, 2320, 2388, 2994
FalI AAGNNNNNCTT 4 cut(s) 454, 486, 2010, 2042
FaqI GGGAC 5 cut(s) 578, 965, 1932, 1933, 2787
FauI CCCGC 4 cut(s) 447, 525, 834, 2367
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 2449
Fnu4HI GCNGC 5 cut(s) 258, 573, 1383, 1398, 1794
FriOI GRGCYC 1 cut(s) 1998
Fsp4HI GCNGC 5 cut(s) 258, 573, 1383, 1398, 1794
FspBI CTAG 2 cut(s) 677, 2748
GlaI GCGC 5 cut(s) 548, 2744, 2746, 3119, 3121
GluI GCNGC 5 cut(s) 258, 573, 1383, 1398, 1794
GsaI CCCAGC 2 cut(s) 963, 2934
GsuI CTGGAG 5 cut(s) 102, 184, 743, 1175, 1857
HaeIII GGCC 6 cut(s) 398, 935, 1331, 1577, 2459, 2837
HapII CCGG 7 cut(s) 71, 222, 1145, 1287, 1332, 1730, 2218
HhaI GCGC 5 cut(s) 549, 2745, 2747, 3120, 3122
Hin6I GCGC 5 cut(s) 547, 2743, 2745, 3118, 3120
HinP1I GCGC 5 cut(s) 547, 2743, 2745, 3118, 3120
HincII GTYRAC 2 cut(s) 588, 2436
HindII GTYRAC 2 cut(s) 588, 2436
HindIII AAGCTT 2 cut(s) 137, 993
HpaI GTTAAC 1 cut(s) 588
HpaII CCGG 7 cut(s) 71, 222, 1145, 1287, 1332, 1730, 2218
HphI GGTGA 8 cut(s) 280, 335, 439, 998, 1133, 1292, 1544, 2441
Hpy166II GTNNAC 7 cut(s) 436, 588, 1362, 1535, 2436, 2450, 2920
Hpy8I GTNNAC 7 cut(s) 436, 588, 1362, 1535, 2436, 2450, 2920
Hpy99I CGWCG 2 cut(s) 798, 1377
HpyAV CCTTC 4 cut(s) 388, 2000, 2827, 3107
HpyCH4III ACNGT 9 cut(s) 384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
HpyCH4IV ACGT 5 cut(s) 26, 175, 771, 793, 1345
HpyF10VI GCNNNNNNNGC 5 cut(s) 544, 775, 1775, 2411, 2609
HpyF3I CTNAG 9 cut(s) 66, 1683, 1915, 1971, 2247, 2408, 2487, 2935, 2946
HpySE526I ACGT 5 cut(s) 26, 175, 771, 793, 1345
HspAI GCGC 5 cut(s) 547, 2743, 2745, 3118, 3120
Kpn2I TCCGGA 1 cut(s) 221
KpnI GGTACC 1 cut(s) 2270
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 520
KspAI GTTAAC 1 cut(s) 588
LmnI GCTCC 6 cut(s) 6, 535, 1194, 1717, 2152, 3055
Lsp1109I GCAGC 5 cut(s) 244, 584, 1369, 1384, 1805
LweI GCATC 7 cut(s) 289, 874, 1115, 1669, 1780, 1841, 2171
MaeI CTAG 2 cut(s) 677, 2748
MaeII ACGT 5 cut(s) 26, 175, 771, 793, 1345
MaeIII GTNAC 7 cut(s) 806, 832, 1121, 1801, 1822, 2708, 3013
MbiI CCGCTC 1 cut(s) 532
MfeI CAATTG 1 cut(s) 1797
MflI RGATCY 2 cut(s) 1540, 2885
MhlI GDGCHC 5 cut(s) 210, 771, 1364, 1998, 2307
MlsI TGGCCA 1 cut(s) 935
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 6 cut(s) 362, 412, 506, 1169, 2141, 2769
MmeI TCCRAC 1 cut(s) 1161
Mox20I TGGCCA 1 cut(s) 935
MroI TCCGGA 1 cut(s) 221
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 2 cut(s) 383, 1591
MscI TGGCCA 1 cut(s) 935
MseI TTAA 9 cut(s) 98, 459, 587, 671, 698, 803, 1650, 2417, 2670
MslI CAYNNNNRTG 1 cut(s) 2704
Msp20I TGGCCA 1 cut(s) 935
MspA1I CMGCKG 2 cut(s) 122, 1883
MspI CCGG 7 cut(s) 71, 222, 1145, 1287, 1332, 1730, 2218
MspR9I CCNGG 7 cut(s) 358, 440, 956, 1193, 1731, 2088, 2205
MunI CAATTG 1 cut(s) 1797
MvaI CCWGG 6 cut(s) 358, 440, 956, 1193, 2088, 2205
MvnI CGCG 2 cut(s) 2745, 3120
MwoI GCNNNNNNNGC 5 cut(s) 544, 775, 1775, 2411, 2609
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 1731
NdeI CATATG 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 70
NlaIV GGNNCC 4 cut(s) 953, 2202, 2268, 2358
NmeAIII GCCGAG 2 cut(s) 1957, 2485
NmuCI GTSAC 2 cut(s) 1121, 1822
OliI CACNNNNGTG 1 cut(s) 2704
PaeR7I CTCGAG 2 cut(s) 400, 2768
PasI CCCWGGG 1 cut(s) 2087
PauI GCGCGC 2 cut(s) 2743, 3118
PceI AGGCCT 1 cut(s) 398
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 383, 1591
PfeI GAWTC 7 cut(s) 146, 368, 859, 877, 973, 1706, 3083
PflMI CCANNNNNTGG 2 cut(s) 2123, 2980
PfoI TCCNGGA 3 cut(s) 356, 1191, 2203
PinAI ACCGGT 1 cut(s) 1286
PkrI GCNGC 5 cut(s) 259, 574, 1384, 1399, 1795
PleI GAGTC 6 cut(s) 361, 411, 505, 1168, 2140, 2769
PmaCI CACGTG 1 cut(s) 772
PmlI CACGTG 1 cut(s) 772
PpsI GAGTC 6 cut(s) 361, 411, 505, 1168, 2140, 2769
Ppu21I YACGTR 2 cut(s) 176, 772
PpuMI RGGWCCY 2 cut(s) 952, 2201
PsiI TTATAA 1 cut(s) 2828
Psp5II RGGWCCY 2 cut(s) 952, 2201
Psp6I CCWGG 6 cut(s) 356, 438, 954, 1191, 2086, 2203
PspCI CACGTG 1 cut(s) 772
PspEI GGTNACC 1 cut(s) 1121
PspFI CCCAGC 2 cut(s) 959, 2930
PspGI CCWGG 6 cut(s) 356, 438, 954, 1191, 2086, 2203
PspN4I GGNNCC 4 cut(s) 953, 2202, 2268, 2358
PspPI GGNCC 7 cut(s) 156, 224, 952, 1084, 1576, 2201, 2356
PspPPI RGGWCCY 2 cut(s) 952, 2201
PspXI VCTCGAGB 2 cut(s) 400, 2768
PstI CTGCAG 1 cut(s) 1032
PstNI CAGNNNCTG 1 cut(s) 1778
PsuI RGATCY 2 cut(s) 1540, 2885
PteI GCGCGC 2 cut(s) 2743, 3118
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 5 cut(s) 86, 435, 872, 2268, 2513
RsaNI GTAC 5 cut(s) 85, 434, 871, 2267, 2512
RseI CAYNNNNRTG 1 cut(s) 2704
Rsr2I CGGWCCG 1 cut(s) 224
RsrII CGGWCCG 1 cut(s) 224
SaqAI TTAA 9 cut(s) 98, 459, 587, 671, 698, 803, 1650, 2417, 2670
SatI GCNGC 5 cut(s) 258, 573, 1383, 1398, 1794
Sau96I GGNCC 7 cut(s) 156, 224, 952, 1084, 1576, 2201, 2356
SchI GAGTC 6 cut(s) 362, 412, 506, 1169, 2141, 2769
ScrFI CCNGG 7 cut(s) 358, 440, 956, 1193, 1731, 2088, 2205
SduI GDGCHC 5 cut(s) 210, 771, 1364, 1998, 2307
SfaNI GCATC 7 cut(s) 289, 874, 1115, 1669, 1780, 1841, 2171
SfcI CTRYAG 3 cut(s) 946, 1028, 1552
Sfr274I CTCGAG 2 cut(s) 400, 2768
SinI GGWCC 6 cut(s) 156, 224, 952, 1084, 2201, 2356
SlaI CTCGAG 2 cut(s) 400, 2768
SmiMI CAYNNNNRTG 1 cut(s) 2704
SmlI CTYRAG 7 cut(s) 400, 824, 1157, 1410, 1663, 2660, 2768
SmoI CTYRAG 7 cut(s) 400, 824, 1157, 1410, 1663, 2660, 2768
SpeI ACTAGT 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 9 cut(s) 454, 532, 841, 1148, 1883, 2360, 2865, 2909, 3053
SspI AATATT 4 cut(s) 195, 695, 1893, 2571
SspMI CTAG 2 cut(s) 677, 2748
StuI AGGCCT 1 cut(s) 398
StyD4I CCNGG 7 cut(s) 356, 438, 954, 1191, 1729, 2086, 2203
StyI CCWWGG 5 cut(s) 1528, 2117, 2320, 2388, 2994
TaaI ACNGT 9 cut(s) 384, 1486, 1556, 2266, 2397, 2579, 2616, 2899, 2917
TaiI ACGT 5 cut(s) 29, 178, 774, 796, 1348
TaqI TCGA 8 cut(s) 401, 1163, 1461, 1633, 1639, 2185, 2769, 2790
TaqII GACCGA 1 cut(s) 241
TatI WGTACW 4 cut(s) 84, 433, 870, 2511
TfiI GAWTC 7 cut(s) 146, 368, 859, 877, 973, 1706, 3083
Tru1I TTAA 9 cut(s) 98, 459, 587, 671, 698, 803, 1650, 2417, 2670
Tru9I TTAA 9 cut(s) 98, 459, 587, 671, 698, 803, 1650, 2417, 2670
TscAI CASTG 6 cut(s) 787, 1032, 1561, 1699, 1815, 2635
TseFI GTSAC 2 cut(s) 1121, 1822
TseI GCWGC 5 cut(s) 257, 572, 1382, 1397, 1793
Tsp45I GTSAC 2 cut(s) 1121, 1822
TspDTI ATGAA 9 cut(s) 429, 495, 672, 753, 986, 1087, 2117, 2664, 2753
TspGWI ACGGA 2 cut(s) 2467, 3032
TspRI CASTG 6 cut(s) 787, 1032, 1561, 1699, 1815, 2635
Van91I CCANNNNNTGG 2 cut(s) 2123, 2980
VneI GTGCAC 1 cut(s) 1360
VpaK11BI GGWCC 6 cut(s) 156, 224, 952, 1084, 2201, 2356
XapI RAATTY 6 cut(s) 179, 1523, 1725, 1986, 2274, 2620
XcmI CCANNNNNNNNNTGG 1 cut(s) 1133
XhoI CTCGAG 2 cut(s) 400, 2768
XmiI GTMKAC 1 cut(s) 2449
XmnI GAANNNNTTC 2 cut(s) 383, 1591
XspI CTAG 2 cut(s) 677, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.