Rroxscaffold_3G00257520

GTPase activator activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009686
Physical Location & Seq
Forward (+)
51879306 .. 51880354
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257520.1

Sequence Viewer

Length: 405 bp
ATGGCGCCTCGAGGGGTCCTTGACGTTTCAGTTATTGGTGGAAGAAATCTAAAAAATCCGGATAAAGTGGGTAAAGTAATGGTCATCGTCAACTATAAAGATCAGGAGCATAGGACCCAACCCGTAGTATGTCATGGGACAGATCCAGAGTGGAATGAGCAACTTCTTTTCACCATAGCTGATTCAGTTGAAGATGTCAGGCTTCGGGTCCATGACAGGGATTACGTTACTGATGAAGAAGTTGGACATTCCTCTATTCCATTAAACACCCTACTTGAGTCGGGGTACGAAGCGCAAATCGAGGCCCATCCATATGATGTATCCAGGGGAGGTGACACCTATGGAGAAATTATCGTTGCCCTCCATTTTCATCCAGAGCCTGGCCATGATAAAGATCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 C2 EF-hand_8

Protein Analysis

134

Amino Acids

15.04

Weight (kDa)

4.83

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 94 4.6e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 380
AccIII TCCGGA 1 cut(s) 58
AclWI GGATC 1 cut(s) 137
AcoI YGGCCR 1 cut(s) 382
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 217, 380
AgsI TTSAA 1 cut(s) 191
AjnI CCWGG 2 cut(s) 323, 379
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 9
Aor13HI TCCGGA 1 cut(s) 58
AoxI GGCC 2 cut(s) 303, 382
AspLEI GCGC 2 cut(s) 7, 295
AspS9I GGNCC 4 cut(s) 16, 114, 208, 304
AsuHPI GGTGA 2 cut(s) 163, 344
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 3 cut(s) 16, 114, 208
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 1 cut(s) 4
BccI CCATC 1 cut(s) 315
BciT130I CCWGG 2 cut(s) 325, 381
BciVI GTATCC 1 cut(s) 331
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 325, 381
Bme18I GGWCC 3 cut(s) 16, 114, 208
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 4 cut(s) 16, 114, 208, 304
BmiI GGNNCC 4 cut(s) 6, 17, 116, 209
BmrFI CCNGG 2 cut(s) 325, 381
BpuEI CTTGAG 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 380
Bse8I GATNNNNATC 1 cut(s) 393
BseAI TCCGGA 1 cut(s) 58
BseBI CCWGG 2 cut(s) 325, 381
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 2 cut(s) 307, 370
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 217, 380
BshFI GGCC 2 cut(s) 305, 384
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 217, 380
BsmFI GGGAC 1 cut(s) 151
BsnI GGCC 2 cut(s) 305, 384
BsoBI CYCGRG 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 58
Bsp143I GATC 3 cut(s) 100, 142, 394
BspANI GGCC 2 cut(s) 305, 384
BspEI TCCGGA 1 cut(s) 58
BspLI GGNNCC 4 cut(s) 6, 17, 116, 209
BspPI GGATC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 3 cut(s) 100, 142, 394
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 325, 381
BstACI GRCGYC 1 cut(s) 5
BstF5I GGATG 2 cut(s) 307, 370
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 295
BstKTI GATC 3 cut(s) 103, 145, 397
BstMBI GATC 3 cut(s) 100, 142, 394
BstNI CCWGG 2 cut(s) 325, 381
BstSCI CCNGG 2 cut(s) 323, 379
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 305, 384
BtsCI GGATG 2 cut(s) 307, 370
CaiI CAGNNNCTG 1 cut(s) 380
CfoI GCGC 2 cut(s) 7, 295
Cfr13I GGNCC 4 cut(s) 16, 114, 208, 304
Csp6I GTAC 1 cut(s) 286
CviAII CATG 3 cut(s) 134, 212, 386
CviJI RGCY 5 cut(s) 179, 202, 305, 379, 384
CviKI_1 RGCY 5 cut(s) 179, 202, 305, 379, 384
CviQI GTAC 1 cut(s) 286
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 102, 144, 396
DpnII GATC 3 cut(s) 100, 142, 394
EaeI YGGCCR 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 16, 114, 208
Eco88I CYCGRG 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 16, 114
EcoRII CCWGG 2 cut(s) 323, 379
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 3 cut(s) 137, 215, 389
FaqI GGGAC 1 cut(s) 151
FatI CATG 3 cut(s) 133, 211, 385
FauNDI CATATG 1 cut(s) 313
FokI GGATG 2 cut(s) 294, 357
GlaI GCGC 2 cut(s) 6, 294
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 305, 384
HapII CCGG 1 cut(s) 59
HhaI GCGC 2 cut(s) 7, 295
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 137, 215, 389
Hin6I GCGC 2 cut(s) 5, 293
HinP1I GCGC 2 cut(s) 5, 293
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 2 cut(s) 182, 278
HpaII CCGG 1 cut(s) 59
HphI GGTGA 2 cut(s) 163, 344
Hpy166II GTNNAC 1 cut(s) 91
Hpy188III TCNNGA 5 cut(s) 59, 104, 146, 374, 398
Hpy8I GTNNAC 1 cut(s) 91
HpyCH4IV ACGT 2 cut(s) 24, 225
HpySE526I ACGT 2 cut(s) 24, 225
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 137, 215, 389
HspAI GCGC 2 cut(s) 5, 293
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 58
Kzo9I GATC 3 cut(s) 100, 142, 394
LmnI GCTCC 1 cut(s) 106
MaeII ACGT 2 cut(s) 24, 225
MaeIII GTNAC 2 cut(s) 226, 332
MalI GATC 3 cut(s) 102, 144, 396
MboI GATC 3 cut(s) 100, 142, 394
MboII GAAGA 3 cut(s) 54, 203, 248
MflI RGATCY 1 cut(s) 142
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 384
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 6 cut(s) 5, 18, 262, 295, 323, 371
Mox20I TGGCCA 1 cut(s) 384
MroI TCCGGA 1 cut(s) 58
MscI TGGCCA 1 cut(s) 384
MseI TTAA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 384
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 325, 381
MvaI CCWGG 2 cut(s) 325, 381
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 313
NdeII GATC 3 cut(s) 100, 142, 394
NlaIII CATG 3 cut(s) 137, 215, 389
NlaIV GGNNCC 4 cut(s) 6, 17, 116, 209
NmuCI GTSAC 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 9
PfeI GAWTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 380
PleI GAGTC 1 cut(s) 286
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 2 cut(s) 16, 114
Psp5II RGGWCCY 2 cut(s) 16, 114
Psp6I CCWGG 2 cut(s) 323, 379
PspGI CCWGG 2 cut(s) 323, 379
PspN4I GGNNCC 4 cut(s) 6, 17, 116, 209
PspPI GGNCC 4 cut(s) 16, 114, 208, 304
PspPPI RGGWCCY 2 cut(s) 16, 114
PspXI VCTCGAGB 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 312
SaqAI TTAA 1 cut(s) 263
Sau3AI GATC 3 cut(s) 100, 142, 394
Sau96I GGNCC 4 cut(s) 16, 114, 208, 304
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 325, 381
SetI ASST 5 cut(s) 27, 181, 228, 334, 341
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 3 cut(s) 16, 114, 208
SlaI CTCGAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 9, 275
SmoI CTYRAG 2 cut(s) 9, 275
Sse9I AATT 1 cut(s) 348
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 2 cut(s) 323, 379
TaiI ACGT 2 cut(s) 27, 228
TaqI TCGA 2 cut(s) 10, 300
TasI AATT 1 cut(s) 348
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TseFI GTSAC 1 cut(s) 332
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 2 cut(s) 249, 359
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 16, 114, 208
XhoI CTCGAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.