FvH4_5g02791

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005443
Physical Location & Seq
Reverse (-)
1679593 .. 1681430
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02791.t1

Sequence Viewer

Length: 648 bp
ATGGGAGGCTGCTGTTGTTGTTCTTCCAAGGCAACCGAGCTGAGGGCTGCGCCTACGTATTACTATGTTTATCCGAGGACTTCTGAAGAGCACGTGCCCTTGTCATCTCGCCAGGGTGCCTCTGCAATCTCCACAGGCCTCCTTGTAGATACGAACCTGGATACATCAATACCTGACACTTACAGACCACCTCCTGCCCCTATTCCATATGATGTGTCAGCAGGGCGTCCTCAAACTCCACCAGTGGCCCAAGATATTTCGGGCAACAAGAGTGATTCAACATTGCAGACAACAACTTCTGATTCTGTTCAACAAGCACCTGGTGACAATCCTCAAGAAACTTCAAACAAGTGTGAAGACCTGAAGGGTTCAGACGACAAAGCAGACACCAAGTTTGAGCTTGATCCAATTAAGAAGGCAGATGTTGAACTTTCAAAGCCAGTGGACTCTGTTGCTTTAGTTATTGAGGAGGAGGATGCTTGCCCCACCTGCTTGGAAGAGTATGATGAAGAGAATCCGAGAATCACCACGAAATGCGACCACCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAAAGAAGTGACACATGTCCTGTGTGTGATAAGGTTTACCTTGCTCAACATTTGCGTAGTTTTGTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

216

Amino Acids

23.59

Weight (kDa)

4.54

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 158 - 201 2.1e-11 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 159 - 205 4.4e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 160 - 201 1.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 161 - 201 3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 161 - 201 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 161 - 203 5.4e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 161 - 199 6.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 116
AclWI GGATC 1 cut(s) 398
AcuI CTGAAG 2 cut(s) 105, 383
AcvI CACGTG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 226
AdeI CACNNNGTG 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 590
AgsI TTSAA 6 cut(s) 279, 311, 345, 428, 435, 569
AjnI CCWGG 3 cut(s) 111, 156, 319
AluBI AGCT 2 cut(s) 40, 400
AluI AGCT 2 cut(s) 40, 400
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 1 cut(s) 398
AoxI GGCC 2 cut(s) 136, 246
ApeKI GCWGC 2 cut(s) 9, 47
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 335, 517, 542
BaeGI GKGCMC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 116
BbrPI CACGTG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 34
BccI CCATC 1 cut(s) 568
BciT130I CCWGG 3 cut(s) 113, 158, 321
BciVI GTATCC 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 2 cut(s) 10, 48
BlsI GCNGC 2 cut(s) 11, 49
Bme1390I CCNGG 3 cut(s) 113, 158, 321
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 3 cut(s) 113, 158, 321
BmsI GCATC 1 cut(s) 466
BoxI GACNNNNGTC 1 cut(s) 591
BpiI GAAGAC 1 cut(s) 363
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 318
BsaAI YACGTR 2 cut(s) 57, 94
BsaHI GRCGYC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 27, 74, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 242, 440
Bse3DI GCAATG 1 cut(s) 281
BseBI CCWGG 3 cut(s) 113, 158, 321
BseDI CCNNGG 3 cut(s) 27, 74, 112
BseGI GGATG 2 cut(s) 481, 579
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 2 cut(s) 242, 440
BseRI GAGGAG 2 cut(s) 482, 485
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 1 cut(s) 34
BshFI GGCC 2 cut(s) 138, 248
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 42
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 2 cut(s) 138, 248
Bsp1286I GDGCHC 2 cut(s) 93, 99
Bsp143I GATC 1 cut(s) 403
BspANI GGCC 2 cut(s) 138, 248
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 281
BsrI ACTGG 2 cut(s) 242, 440
BssECI CCNNGG 3 cut(s) 27, 74, 112
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 3 cut(s) 113, 158, 321
Bst6I CTCTTC 3 cut(s) 81, 492, 504
BstACI GRCGYC 1 cut(s) 226
BstBAI YACGTR 2 cut(s) 57, 94
BstC8I GCNNGC 2 cut(s) 481, 559
BstDEI CTNAG 1 cut(s) 41
BstF5I GGATG 2 cut(s) 481, 579
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 1 cut(s) 406
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstNI CCWGG 3 cut(s) 113, 158, 321
BstNSI RCATGY 1 cut(s) 594
BstPAI GACNNNNGTC 1 cut(s) 591
BstSCI CCNGG 3 cut(s) 111, 156, 319
BstSLI GKGCMC 1 cut(s) 99
BstSNI TACGTA 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 363
BstXI CCANNNNNNTGG 1 cut(s) 493
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 2 cut(s) 138, 248
BtsCI GGATG 2 cut(s) 481, 579
BtsIMutI CAGTG 2 cut(s) 249, 447
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 481, 559
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 215
CsiI ACCWGGT 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 1 cut(s) 591
CviJI RGCY 7 cut(s) 9, 40, 47, 138, 248, 400, 439
CviKI_1 RGCY 7 cut(s) 9, 40, 47, 138, 248, 400, 439
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DraIII CACNNNGTG 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 81, 492, 504
EarI CTCTTC 3 cut(s) 81, 492, 504
Eco105I TACGTA 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 105, 383
Eco72I CACGTG 1 cut(s) 94
EcoRII CCWGG 3 cut(s) 111, 156, 319
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 1 cut(s) 594
FaiI YATR 5 cut(s) 66, 208, 210, 504, 592
FatI CATG 1 cut(s) 590
FauNDI CATATG 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 10, 48
FokI GGATG 2 cut(s) 488, 586
Fsp4HI GCNGC 2 cut(s) 10, 48
GlaI GCGC 1 cut(s) 51
GluI GCNGC 2 cut(s) 10, 48
HaeIII GGCC 2 cut(s) 138, 248
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 1 cut(s) 594
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 5 cut(s) 275, 302, 446, 514, 522
HphI GGTGA 3 cut(s) 335, 517, 542
Hpy166II GTNNAC 2 cut(s) 445, 613
Hpy188I TCNGA 5 cut(s) 75, 85, 301, 373, 519
Hpy188III TCNNGA 2 cut(s) 335, 566
Hpy8I GTNNAC 2 cut(s) 445, 613
HpyAV CCTTC 2 cut(s) 358, 409
HpyCH4IV ACGT 2 cut(s) 56, 93
HpyCH4V TGCA 3 cut(s) 125, 286, 561
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 2 cut(s) 56, 93
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 1 cut(s) 594
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 1 cut(s) 403
LguI GCTCTTC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 1 cut(s) 466
MabI ACCWGGT 1 cut(s) 319
MaeII ACGT 2 cut(s) 56, 93
MaeIII GTNAC 2 cut(s) 323, 584
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 5 cut(s) 15, 98, 368, 509, 521
MhlI GDGCHC 2 cut(s) 93, 99
MluCI AATT 1 cut(s) 408
MlyI GAGTC 1 cut(s) 440
MseI TTAA 1 cut(s) 411
MspR9I CCNGG 3 cut(s) 113, 158, 321
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 3 cut(s) 113, 158, 321
MwoI GCNNNNNNNGC 1 cut(s) 489
NdeI CATATG 1 cut(s) 208
NdeII GATC 1 cut(s) 403
NlaIII CATG 1 cut(s) 594
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 2 cut(s) 323, 584
NspI RCATGY 1 cut(s) 594
PaqCI CACCTGC 1 cut(s) 497
PceI AGGCCT 1 cut(s) 138
PciI ACATGT 1 cut(s) 590
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 1 cut(s) 561
PfeI GAWTC 4 cut(s) 275, 302, 514, 522
PkrI GCNGC 2 cut(s) 11, 49
PleI GAGTC 1 cut(s) 440
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
PpsI GAGTC 1 cut(s) 440
Ppu21I YACGTR 2 cut(s) 57, 94
PscI ACATGT 1 cut(s) 590
PshAI GACNNNNGTC 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 111, 156, 319
PspCI CACGTG 1 cut(s) 94
PspGI CCWGG 3 cut(s) 111, 156, 319
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 247
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 10, 48
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 113, 158, 321
SduI GDGCHC 2 cut(s) 93, 99
SexAI ACCWGGT 1 cut(s) 319
SfaNI GCATC 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
SnaBI TACGTA 1 cut(s) 57
Sse9I AATT 1 cut(s) 408
SseBI AGGCCT 1 cut(s) 138
StuI AGGCCT 1 cut(s) 138
StyD4I CCNGG 3 cut(s) 111, 156, 319
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 2 cut(s) 59, 96
TasI AATT 1 cut(s) 408
TfiI GAWTC 4 cut(s) 275, 302, 514, 522
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 249, 447
TseFI GTSAC 2 cut(s) 323, 584
TseI GCWGC 2 cut(s) 9, 47
Tsp45I GTSAC 2 cut(s) 323, 584
TspDTI ATGAA 1 cut(s) 522
TspRI CASTG 2 cut(s) 249, 447
XceI RCATGY 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.