pycom06g08340

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005443
Physical Location & Seq
Reverse (-)
13452852 .. 13454063
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08340.3

Sequence Viewer

Length: 639 bp
ATGGGAGGCTGCTGCTGCTGTTCTTCCAAAGGAACGGAGCTAAATGCTGCACCAACATACTATTACTTTCCAAGGACATTGGATGAGCATGTTCCTTTATCGTCTCGCCAAGGAGGTGCCTCTGCAGTCTCTACTGGACTCCTAGTTGATACAAATTTGGATACATCAATACCTGACACTTATAGACCTCCTCCTGCCCCTATTCCATATGATGTGGCTATAGAGGATCCTCATACTCCACCATCGGTGCAAGAGATTTGTGGCAGCAAGAGTGAATCAGCATTGCAGACAGCAACAAATTCTGATTCTGTTCAAGAAGCAGTTGGTGGAAATACCCGAGAAGTTTCACCCAAGTGTGAAGACCTGAAGGGTGCGGACTGCAAAGCCCAAACTGATTCAGAACTTGATCCGTTGAAGAAGTCAGAAGTTGAACTTGCGAAATCAGTGGAATCTATTACTTTAACAATGGAGGAAGAGGATCTTTGCCCCACCTGTTTGGAAGAGTATGATCCACAAAATCCAAAAATTACCACAAAATGCGATCATCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAACGAAGTGACACATGTCCTGTGGGTGATCAGGAAATGATATTTGAGCCTCCAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

213

Amino Acids

23.04

Weight (kDa)

4.35

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 1 cut(s) 374
AclWI GGATC 5 cut(s) 221, 234, 401, 486, 503
AcsI RAATTY 2 cut(s) 154, 298
AcuI CTGAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 4 cut(s) 314, 415, 431, 572
AleI CACNNNNGTG 1 cut(s) 352
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 108, 133
AlwI GGATC 5 cut(s) 221, 234, 401, 486, 503
Ama87I CYCGRG 1 cut(s) 336
ApeKI GCWGC 5 cut(s) 9, 12, 15, 47, 264
ApoI RAATTY 2 cut(s) 154, 298
AsuHPI GGTGA 3 cut(s) 339, 545, 617
AvaI CYCGRG 1 cut(s) 336
BamHI GGATCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 2, 34, 276
BccI CCATC 2 cut(s) 250, 571
BciVI GTATCC 1 cut(s) 154
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 108, 133
BfaI CTAG 2 cut(s) 143, 637
BfmI CTRYAG 2 cut(s) 123, 219
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 5 cut(s) 10, 13, 16, 48, 265
BlsI GCNGC 5 cut(s) 11, 14, 17, 49, 266
BmeT110I CYCGRG 1 cut(s) 336
BmiI GGNNCC 2 cut(s) 118, 228
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 71, 109
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 71, 109
BseGI GGATG 2 cut(s) 88, 582
BseMI GCAATG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 3 cut(s) 2, 34, 276
BsgI GTGCAG 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 336
BsmAI GTCTC 2 cut(s) 108, 133
BsmBI CGTCTC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 336
Bsp143I GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BspACI CCGC 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 118, 228
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 5 cut(s) 221, 234, 401, 486, 503
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 281
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 71, 109
BssMI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BssT1I CCWWGG 2 cut(s) 71, 109
Bst6I CTCTTC 2 cut(s) 468, 495
BstC8I GCNNGC 1 cut(s) 562
BstF5I GGATG 2 cut(s) 88, 582
BstKTI GATC 6 cut(s) 229, 409, 481, 511, 544, 610
BstMAI GTCTC 2 cut(s) 108, 133
BstMBI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNSI RCATGY 2 cut(s) 92, 597
BstPAI GACNNNNGTC 1 cut(s) 594
BstSFI CTRYAG 2 cut(s) 123, 219
BstV1I GCAGC 3 cut(s) 2, 34, 276
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 226, 478
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 2 cut(s) 226, 478
BsuI GTATCC 1 cut(s) 154
BtsCI GGATG 2 cut(s) 88, 582
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 562
CviAII CATG 2 cut(s) 89, 594
CviJI RGCY 5 cut(s) 9, 40, 218, 386, 628
CviKI_1 RGCY 5 cut(s) 9, 40, 218, 386, 628
DpnI GATC 6 cut(s) 228, 408, 480, 510, 543, 609
DpnII GATC 6 cut(s) 226, 406, 478, 508, 541, 607
Eam1104I CTCTTC 2 cut(s) 468, 495
EarI CTCTTC 2 cut(s) 468, 495
Eco130I CCWWGG 2 cut(s) 71, 109
Eco57I CTGAAG 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 71, 109
ErhI CCWWGG 2 cut(s) 71, 109
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 2 cut(s) 92, 597
FaiI YATR 9 cut(s) 58, 90, 183, 208, 210, 221, 234, 507, 595
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 465, 497
FatI CATG 2 cut(s) 88, 593
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 5 cut(s) 10, 13, 16, 48, 265
FokI GGATG 2 cut(s) 95, 589
Fsp4HI GCNGC 5 cut(s) 10, 13, 16, 48, 265
FspBI CTAG 2 cut(s) 143, 637
GluI GCNGC 5 cut(s) 10, 13, 16, 48, 265
Hin1II CATG 2 cut(s) 92, 597
HinfI GANTC 5 cut(s) 138, 275, 305, 395, 449
HphI GGTGA 3 cut(s) 339, 545, 617
Hpy188I TCNGA 3 cut(s) 304, 400, 424
Hpy188III TCNNGA 3 cut(s) 314, 569, 611
HpyAV CCTTC 1 cut(s) 361
HpyCH4V TGCA 6 cut(s) 50, 125, 250, 286, 381, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
Hsp92II CATG 2 cut(s) 92, 597
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 6 cut(s) 226, 406, 478, 508, 541, 607
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 7 cut(s) 120, 186, 207, 377, 505, 596, 612
Lsp1109I GCAGC 3 cut(s) 2, 34, 276
MaeI CTAG 2 cut(s) 143, 637
MaeIII GTNAC 1 cut(s) 587
MalI GATC 6 cut(s) 228, 408, 480, 510, 543, 609
MboI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
MboII GAAGA 5 cut(s) 15, 371, 427, 485, 512
MflI RGATCY 2 cut(s) 226, 478
MluCI AATT 3 cut(s) 154, 298, 525
MlyI GAGTC 1 cut(s) 132
MnlI CCTC 9 cut(s) 107, 130, 198, 201, 217, 240, 463, 469, 639
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 564
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeI CATATG 1 cut(s) 208
NdeII GATC 6 cut(s) 226, 406, 478, 508, 541, 607
NlaIII CATG 2 cut(s) 92, 597
NlaIV GGNNCC 2 cut(s) 118, 228
NmuCI GTSAC 1 cut(s) 587
NspI RCATGY 2 cut(s) 92, 597
OliI CACNNNNGTG 1 cut(s) 352
PciI ACATGT 1 cut(s) 593
PctI GAATGC 1 cut(s) 564
PfeI GAWTC 4 cut(s) 275, 305, 395, 449
PkrI GCNGC 5 cut(s) 11, 14, 17, 49, 266
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PscI ACATGT 1 cut(s) 593
PshAI GACNNNNGTC 1 cut(s) 594
PspN4I GGNNCC 2 cut(s) 118, 228
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 2 cut(s) 226, 478
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 5 cut(s) 10, 13, 16, 48, 265
Sau3AI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
SchI GAGTC 1 cut(s) 132
SetI ASST 7 cut(s) 42, 118, 175, 190, 366, 494, 558
SfcI CTRYAG 2 cut(s) 123, 219
SmiMI CAYNNNNRTG 1 cut(s) 352
Sse9I AATT 3 cut(s) 154, 298, 525
SsiI CCGC 1 cut(s) 374
SspMI CTAG 2 cut(s) 143, 637
StyI CCWWGG 2 cut(s) 71, 109
TasI AATT 3 cut(s) 154, 298, 525
TfiI GAWTC 4 cut(s) 275, 305, 395, 449
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 5 cut(s) 9, 12, 15, 47, 264
Tsp45I GTSAC 1 cut(s) 587
TspGWI ACGGA 2 cut(s) 50, 399
TspRI CASTG 1 cut(s) 450
XapI RAATTY 2 cut(s) 154, 298
XceI RCATGY 2 cut(s) 92, 597
XspI CTAG 2 cut(s) 143, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.