MD06G1087300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005443
Physical Location & Seq
Reverse (-)
21393382 .. 21396196
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1087300.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGGAGGCTGCTGCTGCTGTTCTTCCAAAGGAACGGAGCTAAATGCTGCACCAACATACTATTACTTTCCAAGGACATCAGATGAGCAAGTTCCTTTATCGTCTCGCCAAGGAGGTGCCTCTGCAGTCTCTACTGGGCTCCTAGTTGATACAAATTTGGATACATCAATACCTGACACTTATAGACCACCTCCTGCCCCTATTCCATATGATGTGGCTATAGAAGATCCTCATACTCCACCATCGGTGCAAGAGATTTGTGGCAGCAAGAGTGAATCAGCATTGCAGACAGCAACAAATTCTGATTCGGTTCAAGAAGCAGTTGGTCGAAATACCCGAGAAGCTTCACCCAAGTGTGAAGACCTGAAGGATGCGGACTGCAAAGCCCAAACTGATTCAGAACTTGATCCGTTGAAGAAGTCCGAAGTTGAACTTGAGAAATCAGTGGAATCTTTTACTTTAACAATAGAGGAAGAGGATCTTTGTCCCACCTGTTTGGAAGAGTATGATCCACAAAATCCAAAAATTACCACAAAATGCGATCATCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAACGAAGTGACACATGTCCTGTGTGTGATCAGGAAATGATATTTGAGTCTCCAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

213

Amino Acids

23.25

Weight (kDa)

4.3

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 159 - 202 9.1e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 161 - 202 7.8e-12 Ring finger domain
zf-ANAPC11 PF12861 161 - 209 7.8e-08 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4 PF00097 162 - 202 6.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 162 - 202 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 162 - 204 4.2e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 1 cut(s) 374
AclWI GGATC 4 cut(s) 221, 401, 486, 503
AcsI RAATTY 2 cut(s) 154, 298
AcuI CTGAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 4 cut(s) 314, 415, 431, 572
AleI CACNNNNGTG 1 cut(s) 352
AluBI AGCT 2 cut(s) 40, 344
AluI AGCT 2 cut(s) 40, 344
Alw26I GTCTC 3 cut(s) 108, 133, 633
AlwI GGATC 4 cut(s) 221, 401, 486, 503
Ama87I CYCGRG 1 cut(s) 336
ApeKI GCWGC 5 cut(s) 9, 12, 15, 47, 264
ApoI RAATTY 2 cut(s) 154, 298
AsuHPI GGTGA 2 cut(s) 339, 545
AvaI CYCGRG 1 cut(s) 336
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 2, 34, 276
BccI CCATC 2 cut(s) 250, 571
BciVI GTATCC 1 cut(s) 154
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 3 cut(s) 108, 133, 633
BfaI CTAG 2 cut(s) 143, 637
BfmI CTRYAG 2 cut(s) 123, 219
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 5 cut(s) 10, 13, 16, 48, 265
BlsI GCNGC 5 cut(s) 11, 14, 17, 49, 266
BmeT110I CYCGRG 1 cut(s) 336
BmiI GGNNCC 2 cut(s) 118, 140
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 361
BmuI ACTGGG 1 cut(s) 144
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 71, 109
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 71, 109
BseGI GGATG 2 cut(s) 376, 582
BseMI GCAATG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 2, 34, 276
BsgI GTGCAG 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 471
BsmAI GTCTC 3 cut(s) 108, 133, 633
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BspACI CCGC 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 118, 140
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 4 cut(s) 221, 401, 486, 503
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 281
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 71, 109
BssMI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BssT1I CCWWGG 2 cut(s) 71, 109
Bst6I CTCTTC 2 cut(s) 468, 495
BstC8I GCNNGC 1 cut(s) 562
BstF5I GGATG 2 cut(s) 376, 582
BstKTI GATC 6 cut(s) 229, 409, 481, 511, 544, 610
BstMAI GTCTC 3 cut(s) 108, 133, 633
BstMBI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 597
BstPAI GACNNNNGTC 1 cut(s) 594
BstSFI CTRYAG 2 cut(s) 123, 219
BstV1I GCAGC 3 cut(s) 2, 34, 276
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 226, 478
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 2 cut(s) 226, 478
BsuI GTATCC 1 cut(s) 154
BtsCI GGATG 2 cut(s) 376, 582
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 562
CviAII CATG 1 cut(s) 594
CviJI RGCY 6 cut(s) 9, 40, 139, 218, 344, 386
CviKI_1 RGCY 6 cut(s) 9, 40, 139, 218, 344, 386
DpnI GATC 6 cut(s) 228, 408, 480, 510, 543, 609
DpnII GATC 6 cut(s) 226, 406, 478, 508, 541, 607
Eam1104I CTCTTC 2 cut(s) 468, 495
EarI CTCTTC 2 cut(s) 468, 495
Eco130I CCWWGG 2 cut(s) 71, 109
Eco24I GRGCYC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 71, 109
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 71, 109
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 1 cut(s) 597
FaiI YATR 8 cut(s) 58, 183, 208, 210, 221, 234, 507, 595
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 465, 497
FaqI GGGAC 1 cut(s) 471
FatI CATG 1 cut(s) 593
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 5 cut(s) 10, 13, 16, 48, 265
FokI GGATG 2 cut(s) 383, 589
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 5 cut(s) 10, 13, 16, 48, 265
FspBI CTAG 2 cut(s) 143, 637
GluI GCNGC 5 cut(s) 10, 13, 16, 48, 265
Hin1II CATG 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 5 cut(s) 275, 305, 395, 449, 626
HphI GGTGA 2 cut(s) 339, 545
Hpy188I TCNGA 4 cut(s) 82, 304, 400, 424
Hpy188III TCNNGA 3 cut(s) 314, 569, 611
HpyAV CCTTC 1 cut(s) 361
HpyCH4V TGCA 6 cut(s) 50, 125, 250, 286, 381, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
Hsp92II CATG 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 6 cut(s) 226, 406, 478, 508, 541, 607
LmnI GCTCC 2 cut(s) 37, 144
LpnPI CCDG 7 cut(s) 120, 186, 207, 377, 505, 596, 612
Lsp1109I GCAGC 3 cut(s) 2, 34, 276
LweI GCATC 1 cut(s) 361
MaeI CTAG 2 cut(s) 143, 637
MaeIII GTNAC 1 cut(s) 587
MalI GATC 6 cut(s) 228, 408, 480, 510, 543, 609
MboI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
MboII GAAGA 6 cut(s) 15, 236, 371, 427, 485, 512
MflI RGATCY 2 cut(s) 226, 478
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 3 cut(s) 154, 298, 525
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 6 cut(s) 107, 130, 201, 240, 463, 469
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 564
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeI CATATG 1 cut(s) 208
NdeII GATC 6 cut(s) 226, 406, 478, 508, 541, 607
NlaIII CATG 1 cut(s) 597
NlaIV GGNNCC 2 cut(s) 118, 140
NmuCI GTSAC 1 cut(s) 587
NspI RCATGY 1 cut(s) 597
OliI CACNNNNGTG 1 cut(s) 352
PciI ACATGT 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PctI GAATGC 1 cut(s) 564
PfeI GAWTC 4 cut(s) 275, 305, 395, 449
PkrI GCNGC 5 cut(s) 11, 14, 17, 49, 266
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
PscI ACATGT 1 cut(s) 593
PshAI GACNNNNGTC 1 cut(s) 594
PspN4I GGNNCC 2 cut(s) 118, 140
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 2 cut(s) 226, 478
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 5 cut(s) 10, 13, 16, 48, 265
Sau3AI GATC 6 cut(s) 226, 406, 478, 508, 541, 607
SchI GAGTC 1 cut(s) 635
SduI GDGCHC 1 cut(s) 141
SetI ASST 8 cut(s) 42, 118, 175, 193, 346, 366, 494, 558
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 2 cut(s) 123, 219
SmiMI CAYNNNNRTG 1 cut(s) 352
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 3 cut(s) 154, 298, 525
SsiI CCGC 1 cut(s) 374
SspMI CTAG 2 cut(s) 143, 637
StyI CCWWGG 2 cut(s) 71, 109
TaqI TCGA 1 cut(s) 328
TasI AATT 3 cut(s) 154, 298, 525
TfiI GAWTC 4 cut(s) 275, 305, 395, 449
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 5 cut(s) 9, 12, 15, 47, 264
Tsp45I GTSAC 1 cut(s) 587
TspGWI ACGGA 2 cut(s) 50, 399
TspRI CASTG 1 cut(s) 450
XapI RAATTY 2 cut(s) 154, 298
XceI RCATGY 1 cut(s) 597
XspI CTAG 2 cut(s) 143, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.