MD14G1108200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005443
Physical Location & Seq
Reverse (-)
16872604 .. 16875401
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1108200.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGGAGGCTGCTGCTGCTGTTCATCCAAAGGAACGGAGCTGAATGCTGCACCAACATACTATTACTGTCCAAGGACCTCGGATGAGCACATTCCCTTATCGTCTCACCAAGGAGGTGCCTCTGCACTCTCTACTGGGCTCCTAGTTGATACAAATTTGGATACATCAATACCTGACACTTATAGACCACCTCCTGCCCCTATTCCATATGATGCGGCTTTAGAGCATCCTCATACTCCACCACCGGTGCAAGAGATTTGTGGCAGCAAGAGTGAAGCAGCATTGCAGATAACAACAAATTCTGATTCTGTTCAAGAAGCAGTTGGTGGAAATACCCAAGAAGCTTCACCCAAGTGTGCAGACCTGAAGGATGTGGACTGCAAAGCCCAAACGGATTTGGAACTTGATCCAGTGAATAAGTCAGAAATTGAACTTGCCAAATCAGTGGAATCTATTACTTTAGCAATAGAGGAAGAGGATGTTTGTCCCACTTGTTTGGAAGAGTATGATCCACAAAATCCAAAAATTACTACAAAATGCGATCATCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAACGAAGTGACACATGTCCTGTGTGTGATCAGGAAATGATATTTGAGCCTCCAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

213

Amino Acids

22.98

Weight (kDa)

4.32

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 159 - 202 3.9e-11 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 160 - 208 6.6e-08 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 161 - 202 8.8e-12 Ring finger domain
zf-C3HC4_2 PF13923 162 - 202 6.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 162 - 202 6.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 162 - 200 1.4e-06 RING-type zinc-finger
zf-RING_5 PF14634 162 - 204 4.2e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 1 cut(s) 215
AclWI GGATC 2 cut(s) 401, 503
AcsI RAATTY 2 cut(s) 154, 298
AcuI CTGAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 593
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 3 cut(s) 314, 431, 572
AleI CACNNNNGTG 1 cut(s) 352
AluBI AGCT 2 cut(s) 40, 344
AluI AGCT 2 cut(s) 40, 344
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 2 cut(s) 401, 503
ApeKI GCWGC 6 cut(s) 9, 12, 15, 47, 264, 278
ApoI RAATTY 2 cut(s) 154, 298
AsiGI ACCGGT 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 75
AsuHPI GGTGA 3 cut(s) 98, 339, 545
AvaII GGWCC 1 cut(s) 75
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 4 cut(s) 2, 34, 276, 290
BccI CCATC 1 cut(s) 571
BciVI GTATCC 1 cut(s) 154
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 2 cut(s) 143, 637
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 7 cut(s) 10, 13, 16, 48, 216, 265, 279
BlsI GCNGC 7 cut(s) 11, 14, 17, 49, 217, 266, 280
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 118, 140
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 2 cut(s) 202, 235
BmuI ACTGGG 1 cut(s) 144
BoxI GACNNNNGTC 1 cut(s) 594
BsaJI CCNNGG 3 cut(s) 71, 78, 109
BsaWI WCCGGW 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 139, 410
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 3 cut(s) 71, 78, 109
BseGI GGATG 6 cut(s) 23, 88, 226, 376, 484, 582
BseMI GCAATG 1 cut(s) 281
BseNI ACTGG 2 cut(s) 139, 410
BseXI GCAGC 4 cut(s) 2, 34, 276, 290
BsgI GTGCAG 3 cut(s) 33, 108, 378
BshNI GGYRCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 471
BsmAI GTCTC 1 cut(s) 108
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 49, 564
Bsp1286I GDGCHC 2 cut(s) 90, 141
Bsp143I GATC 4 cut(s) 406, 508, 541, 607
BspACI CCGC 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 118, 140
BspPI GGATC 2 cut(s) 401, 503
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 2 cut(s) 139, 410
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 71, 78, 109
BssMI GATC 4 cut(s) 406, 508, 541, 607
BssT1I CCWWGG 2 cut(s) 71, 109
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 2 cut(s) 468, 495
BstC8I GCNNGC 1 cut(s) 562
BstF5I GGATG 6 cut(s) 23, 88, 226, 376, 484, 582
BstKTI GATC 4 cut(s) 409, 511, 544, 610
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 4 cut(s) 406, 508, 541, 607
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 597
BstPAI GACNNNNGTC 1 cut(s) 594
BstV1I GCAGC 4 cut(s) 2, 34, 276, 290
BstXI CCANNNNNNTGG 2 cut(s) 445, 496
BsuI GTATCC 1 cut(s) 154
BtsCI GGATG 6 cut(s) 23, 88, 226, 376, 484, 582
BtsIMutI CAGTG 2 cut(s) 417, 450
Cac8I GCNNGC 1 cut(s) 562
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 75
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 1 cut(s) 594
CviJI RGCY 7 cut(s) 9, 40, 139, 218, 344, 386, 628
CviKI_1 RGCY 7 cut(s) 9, 40, 139, 218, 344, 386, 628
DpnI GATC 4 cut(s) 408, 510, 543, 609
DpnII GATC 4 cut(s) 406, 508, 541, 607
Eam1104I CTCTTC 2 cut(s) 468, 495
EarI CTCTTC 2 cut(s) 468, 495
Eco130I CCWWGG 2 cut(s) 71, 109
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 75
EcoT14I CCWWGG 2 cut(s) 71, 109
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 71, 109
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 1 cut(s) 597
FaiI YATR 7 cut(s) 58, 183, 208, 210, 234, 507, 595
FaqI GGGAC 1 cut(s) 471
FatI CATG 1 cut(s) 593
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 7 cut(s) 10, 13, 16, 48, 216, 265, 279
FokI GGATG 6 cut(s) 10, 95, 213, 383, 491, 589
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 7 cut(s) 10, 13, 16, 48, 216, 265, 279
FspBI CTAG 2 cut(s) 143, 637
GluI GCNGC 7 cut(s) 10, 13, 16, 48, 216, 265, 279
HapII CCGG 1 cut(s) 245
Hin1II CATG 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 2 cut(s) 305, 449
HpaII CCGG 1 cut(s) 245
HphI GGTGA 3 cut(s) 98, 339, 545
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 82, 304, 424
Hpy188III TCNNGA 3 cut(s) 314, 569, 611
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 7 cut(s) 50, 125, 250, 286, 359, 381, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
Hsp92II CATG 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 4 cut(s) 406, 508, 541, 607
LmnI GCTCC 2 cut(s) 37, 144
LpnPI CCDG 8 cut(s) 120, 186, 207, 258, 377, 423, 596, 612
Lsp1109I GCAGC 4 cut(s) 2, 34, 276, 290
LweI GCATC 2 cut(s) 202, 235
MaeI CTAG 2 cut(s) 143, 637
MaeIII GTNAC 1 cut(s) 587
MalI GATC 4 cut(s) 408, 510, 543, 609
MboI GATC 4 cut(s) 406, 508, 541, 607
MboII GAAGA 2 cut(s) 485, 512
MhlI GDGCHC 2 cut(s) 90, 141
MluCI AATT 4 cut(s) 154, 298, 426, 525
MnlI CCTC 8 cut(s) 88, 107, 130, 201, 240, 463, 469, 639
MslI CAYNNNNRTG 1 cut(s) 352
MspI CCGG 1 cut(s) 245
Mva1269I GAATGC 2 cut(s) 49, 564
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeI CATATG 1 cut(s) 208
NdeII GATC 4 cut(s) 406, 508, 541, 607
NlaIII CATG 1 cut(s) 597
NlaIV GGNNCC 2 cut(s) 118, 140
NmuCI GTSAC 1 cut(s) 587
NspI RCATGY 1 cut(s) 597
OliI CACNNNNGTG 1 cut(s) 352
PciI ACATGT 1 cut(s) 593
PctI GAATGC 2 cut(s) 49, 564
PfeI GAWTC 2 cut(s) 305, 449
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 7 cut(s) 11, 14, 17, 49, 217, 266, 280
PpuMI RGGWCCY 1 cut(s) 75
PscI ACATGT 1 cut(s) 593
PshAI GACNNNNGTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 118, 140
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
RseI CAYNNNNRTG 1 cut(s) 352
SatI GCNGC 7 cut(s) 10, 13, 16, 48, 216, 265, 279
Sau3AI GATC 4 cut(s) 406, 508, 541, 607
Sau96I GGNCC 1 cut(s) 75
SduI GDGCHC 2 cut(s) 90, 141
SetI ASST 8 cut(s) 42, 80, 118, 175, 193, 346, 366, 558
SfaNI GCATC 2 cut(s) 202, 235
SgrAI CRCCGGYG 1 cut(s) 244
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 352
Sse9I AATT 4 cut(s) 154, 298, 426, 525
SsiI CCGC 1 cut(s) 215
SspMI CTAG 2 cut(s) 143, 637
StyI CCWWGG 2 cut(s) 71, 109
TaaI ACNGT 1 cut(s) 68
TasI AATT 4 cut(s) 154, 298, 426, 525
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 2 cut(s) 305, 449
TscAI CASTG 2 cut(s) 417, 450
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 6 cut(s) 9, 12, 15, 47, 264, 278
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 2 cut(s) 50, 407
TspRI CASTG 2 cut(s) 417, 450
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 2 cut(s) 154, 298
XceI RCATGY 1 cut(s) 597
XspI CTAG 2 cut(s) 143, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.