Rroxscaffold_7G00198200

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005443
Physical Location & Seq
Reverse (-)
43258960 .. 43259412
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198200.1

Sequence Viewer

Length: 453 bp
ATGTGGATGCATCAATGCCTCGACGATTATAGACCACCTCCTGCCCCTTTTTCTTTTGATGTGTCGGCAGGGCATCCACGGACTCTACCAAGTGGCCCAAGAAATTTCGGCATCAAGAATGATGCAACATTGCAGACAAACAACTTCGATTCTGTTCAAGAAGCAGCTGGTGACAATCCTCGTGAAACTTCATCCAAGTGTGAAGACCCGAAGAACAAAGTGTACACCAAGTTAGAGCTCGATCCAATTAAGAAGGCGAAGTTGAACTTTCGAAACCAAAGGAAATCTGTTGCTTTAGTCATCGAGGAGAAGGATGTTGGCCCCGACTGCATGGAAGAGTATGATGAAGAAAATCTAAGAAGTGCCACTAAATACGACCACCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAAAGAAGTGACTCATGTCCGTGTGCGTTCAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

150

Amino Acids

17.25

Weight (kDa)

5.73

Isoelectric Point (pI)

55.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 108 - 145 2.7e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 3 cut(s) 158, 265, 407
AluBI AGCT 2 cut(s) 167, 238
AluI AGCT 2 cut(s) 167, 238
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 1 cut(s) 236
AoxI GGCC 2 cut(s) 94, 319
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 95, 320
AsuHPI GGTGA 2 cut(s) 182, 380
AsuII TTCGAA 1 cut(s) 271
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 176
BccI CCATC 1 cut(s) 406
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 95, 320
BmiI GGNNCC 1 cut(s) 322
BmsI GCATC 4 cut(s) 19, 82, 112, 120
BoxI GACNNNNGTC 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 210
Bpu14I TTCGAA 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 5 cut(s) 12, 73, 191, 319, 417
BseMI GCAATG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 176
BshFI GGCC 2 cut(s) 96, 321
BsiHKAI GWGCWC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 2 cut(s) 96, 321
Bsp119I TTCGAA 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 1 cut(s) 241
BspANI GGCC 2 cut(s) 96, 321
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 1 cut(s) 236
BspT104I TTCGAA 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 1 cut(s) 241
BssSI CACGAG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 180
Bst6I CTCTTC 1 cut(s) 330
BstAUI TGTACA 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 1 cut(s) 356
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 5 cut(s) 12, 73, 191, 319, 417
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstPAI GACNNNNGTC 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 96, 321
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 5 cut(s) 12, 73, 191, 319, 417
Cac8I GCNNGC 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 95, 320
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 331, 429
CviJI RGCY 4 cut(s) 96, 167, 238, 321
CviKI_1 RGCY 4 cut(s) 96, 167, 238, 321
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 356
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 2 cut(s) 334, 432
FaiI YATR 4 cut(s) 30, 332, 342, 430
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 2 cut(s) 330, 428
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 5 cut(s) 19, 60, 178, 326, 424
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 96, 321
Hin1II CATG 2 cut(s) 334, 432
HinfI GANTC 3 cut(s) 82, 149, 425
HphI GGTGA 2 cut(s) 182, 380
Hpy166II GTNNAC 2 cut(s) 223, 225
Hpy188III TCNNGA 5 cut(s) 115, 158, 182, 404, 445
Hpy8I GTNNAC 2 cut(s) 223, 225
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 2 cut(s) 247, 304
HpyCH4V TGCA 5 cut(s) 10, 125, 133, 330, 399
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 1 cut(s) 356
Hsp92II CATG 2 cut(s) 334, 432
Kzo9I GATC 1 cut(s) 241
LpnPI CCDG 4 cut(s) 54, 54, 153, 430
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 4 cut(s) 19, 82, 112, 120
MaeIII GTNAC 2 cut(s) 170, 422
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 4 cut(s) 215, 223, 347, 359
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 103, 246
MlyI GAGTC 2 cut(s) 76, 419
MnlI CCTC 4 cut(s) 29, 48, 189, 298
Mph1103I ATGCAT 1 cut(s) 12
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 2 cut(s) 196, 433
MspA1I CMGCKG 1 cut(s) 167
Mva1269I GAATGC 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeII GATC 1 cut(s) 241
NlaIII CATG 2 cut(s) 334, 432
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 170, 422
NsiI ATGCAT 1 cut(s) 12
NspV TTCGAA 1 cut(s) 271
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 2 cut(s) 76, 419
PpsI GAGTC 2 cut(s) 76, 419
PshAI GACNNNNGTC 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 2 cut(s) 95, 320
PsrI GAACNNNNNNTAC 2 cut(s) 206, 238
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 196, 433
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 2 cut(s) 95, 320
SchI GAGTC 2 cut(s) 76, 419
SduI GDGCHC 1 cut(s) 240
SetI ASST 4 cut(s) 40, 169, 240, 393
SfaNI GCATC 4 cut(s) 19, 82, 112, 120
SfuI TTCGAA 1 cut(s) 271
SmiMI CAYNNNNRTG 2 cut(s) 196, 433
Sse9I AATT 2 cut(s) 103, 246
SstI GAGCTC 1 cut(s) 240
TaqI TCGA 5 cut(s) 21, 147, 240, 271, 303
TasI AATT 2 cut(s) 103, 246
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TseFI GTSAC 2 cut(s) 170, 422
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 2 cut(s) 170, 422
TspDTI ATGAA 2 cut(s) 180, 360
TspGWI ACGGA 2 cut(s) 94, 423
XapI RAATTY 1 cut(s) 103
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.