Rroxscaffold_3G00258650

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005443
Physical Location & Seq
Reverse (-)
53103516 .. 53106377
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258650.1

Sequence Viewer

Length: 633 bp
ATGGGAGGGTGCTGTTGTTGTTCTTCCAAAGCAACCGAGCTGAGGGCCGCACCTACATACTACTATGTTTATCCGAGGACATCTGAAGAGCACGTGCCCCTGTCATCTCACCAAGGTGCCTCTGCACTCTCCACAGGCCTCCTTGTTGATACGAACTTGGATACATCAATACCTGACACTTATAGACCACCTCCTGCCCCTATTCCATATGATGTGTCAGCAGGGCGTCCTCAGACTCCACCAGTGGCCCAAGAAATTTCTGGCAACAAAAGTGATGCAACATTGCAGACAACAACAACTTCTGATTCTGTTCAAGAAGCAGCTGGTGACAATCCTCAAGAAACTTCATCCAAGTGTGAAGACCTGAAGTACAAAGTGGACACCAAGTTAGAGCTTGATCCAATTAAGAAGGCAGAAGTTGAACTTTCGAAGCCAGTGGAATCTGTTGCTTTAGTCACTGAGGAGGAGGATGTTTGCCCCACCTGCTTGGAAGAGTATGATGACGAAAATCCAAAAATTACCACGAAATGCGACCACCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAAAGAAGTGACACATGTCCCGTGTGTGATCAGGAAATGATATTTGATCCTCCTATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

210

Amino Acids

23.03

Weight (kDa)

4.38

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 156 - 199 1.4e-11 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 157 - 205 1.6e-08 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 158 - 199 3.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 159 - 199 1.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 159 - 199 2.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 159 - 197 7.7e-07 RING-type zinc-finger
zf-RING_5 PF14634 159 - 201 3.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 1 cut(s) 48
AclWI GGATC 2 cut(s) 392, 611
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 2 cut(s) 105, 386
AcvI CACGTG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 584
AgsI TTSAA 3 cut(s) 314, 422, 563
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 3 cut(s) 40, 323, 394
AluI AGCT 3 cut(s) 40, 323, 394
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 2 cut(s) 392, 611
AoxI GGCC 3 cut(s) 45, 136, 246
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 45, 247
AsuHPI GGTGA 3 cut(s) 101, 338, 536
AsuII TTCGAA 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 116
BbrPI CACGTG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 332
BccI CCATC 1 cut(s) 562
BciVI GTATCC 1 cut(s) 154
BclI TGATCA 1 cut(s) 598
BfuAI ACCTGC 1 cut(s) 491
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 2 cut(s) 48, 321
BlsI GCNGC 2 cut(s) 49, 322
BmgT120I GGNCC 2 cut(s) 45, 247
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 1 cut(s) 265
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 366
Bpu10I CCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 74, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 242, 434
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 74, 112
BseGI GGATG 3 cut(s) 347, 475, 573
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 3 cut(s) 32, 245, 450
BseNI ACTGG 2 cut(s) 242, 434
BseRI GAGGAG 2 cut(s) 476, 479
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 3 cut(s) 47, 138, 248
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 93
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 3 cut(s) 47, 138, 248
Bsp119I TTCGAA 1 cut(s) 428
Bsp1286I GDGCHC 2 cut(s) 93, 99
Bsp143I GATC 3 cut(s) 397, 598, 616
BspACI CCGC 1 cut(s) 48
BspANI GGCC 3 cut(s) 47, 138, 248
BspCNI CTCAG 3 cut(s) 33, 244, 451
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 2 cut(s) 392, 611
BspQI GCTCTTC 1 cut(s) 81
BspT104I TTCGAA 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 281
BsrI ACTGG 2 cut(s) 242, 434
BssECI CCNNGG 2 cut(s) 74, 112
BssMI GATC 3 cut(s) 397, 598, 616
BssNI GRCGYC 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 81, 486
BstACI GRCGYC 1 cut(s) 226
BstBAI YACGTR 1 cut(s) 94
BstBI TTCGAA 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 3 cut(s) 41, 231, 459
BstF5I GGATG 3 cut(s) 347, 475, 573
BstKTI GATC 3 cut(s) 400, 601, 619
BstMBI GATC 3 cut(s) 397, 598, 616
BstMWI GCNNNNNNNGC 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 588
BstPAI GACNNNNGTC 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 366
BstXI CCANNNNNNTGG 1 cut(s) 487
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 3 cut(s) 47, 138, 248
BtsCI GGATG 3 cut(s) 347, 475, 573
BtsIMutI CAGTG 3 cut(s) 249, 441, 456
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 553
Cfr13I GGNCC 2 cut(s) 45, 247
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 370
CviAII CATG 1 cut(s) 585
CviJI RGCY 7 cut(s) 40, 47, 138, 248, 323, 394, 433
CviKI_1 RGCY 7 cut(s) 40, 47, 138, 248, 323, 394, 433
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 41, 231, 459
DpnI GATC 3 cut(s) 399, 600, 618
DpnII GATC 3 cut(s) 397, 598, 616
Eam1104I CTCTTC 2 cut(s) 81, 486
EarI CTCTTC 2 cut(s) 81, 486
Eco130I CCWWGG 1 cut(s) 112
Eco147I AGGCCT 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 105, 386
Eco72I CACGTG 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 588
FaiI YATR 7 cut(s) 58, 66, 183, 208, 210, 498, 586
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 1 cut(s) 573
FatI CATG 1 cut(s) 584
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 48, 321
FokI GGATG 3 cut(s) 334, 482, 580
Fsp4HI GCNGC 2 cut(s) 48, 321
GluI GCNGC 2 cut(s) 48, 321
HaeIII GGCC 3 cut(s) 47, 138, 248
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 1 cut(s) 588
HinfI GANTC 3 cut(s) 235, 305, 440
HphI GGTGA 3 cut(s) 101, 338, 536
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 75, 85, 234, 304
Hpy188III TCNNGA 4 cut(s) 314, 338, 560, 602
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 403
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 4 cut(s) 125, 278, 286, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 483
HpyF3I CTNAG 3 cut(s) 41, 231, 459
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 3 cut(s) 397, 598, 616
LguI GCTCTTC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 1 cut(s) 265
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 3 cut(s) 326, 454, 578
MalI GATC 3 cut(s) 399, 600, 618
MboI GATC 3 cut(s) 397, 598, 616
MboII GAAGA 4 cut(s) 15, 98, 371, 503
MhlI GDGCHC 2 cut(s) 93, 99
MluCI AATT 3 cut(s) 255, 402, 516
MlyI GAGTC 1 cut(s) 229
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 2 cut(s) 114, 352
MspA1I CMGCKG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 555
MwoI GCNNNNNNNGC 1 cut(s) 483
NdeI CATATG 1 cut(s) 208
NdeII GATC 3 cut(s) 397, 598, 616
NlaIII CATG 1 cut(s) 588
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 3 cut(s) 326, 454, 578
NspI RCATGY 1 cut(s) 588
NspV TTCGAA 1 cut(s) 428
OliI CACNNNNGTG 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 491
PceI AGGCCT 1 cut(s) 138
PciI ACATGT 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 305, 440
PkrI GCNGC 2 cut(s) 49, 322
PleI GAGTC 1 cut(s) 229
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
PpsI GAGTC 1 cut(s) 229
Ppu21I YACGTR 1 cut(s) 94
PscI ACATGT 1 cut(s) 584
PshAI GACNNNNGTC 1 cut(s) 585
PspCI CACGTG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 45, 247
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 2 cut(s) 114, 352
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 2 cut(s) 48, 321
Sau3AI GATC 3 cut(s) 397, 598, 616
Sau96I GGNCC 2 cut(s) 45, 247
SchI GAGTC 1 cut(s) 229
SduI GDGCHC 2 cut(s) 93, 99
SfaNI GCATC 1 cut(s) 265
SfuI TTCGAA 1 cut(s) 428
SmiMI CAYNNNNRTG 2 cut(s) 114, 352
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
Sse9I AATT 3 cut(s) 255, 402, 516
SseBI AGGCCT 1 cut(s) 138
SsiI CCGC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 138
StyI CCWWGG 1 cut(s) 112
TaiI ACGT 1 cut(s) 96
TaqI TCGA 1 cut(s) 428
TasI AATT 3 cut(s) 255, 402, 516
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 305, 440
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 3 cut(s) 249, 441, 463
TseFI GTSAC 3 cut(s) 326, 454, 578
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 3 cut(s) 326, 454, 578
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 3 cut(s) 249, 441, 463
XapI RAATTY 1 cut(s) 255
XceI RCATGY 1 cut(s) 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.