FvH4_5g03580

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013503
Physical Location & Seq
Reverse (-)
2096559 .. 2100032
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03580.t1

Sequence Viewer

Length: 1050 bp
ATGTTGAGCTCCACTTGCTCTCACGCCCTTCTCCACCGCCCTTTCCTCCCCTCCTCCATCCCCAGAGCTAAGGCTCCGACCCGACCCGGGGGTTTGTACCCGATTTCCCGGAGTTTAAGGATCTCCGAATCTCATTCCAGGCCCTTCAGCACTAGATGGAGGAAAATTTCATGCTTTAGGCAAGAGGAGTTTTCTTCAGAGACCCAGAAAGCTGAGTATATTGGACACTTGGTGCCGGAGGAAGTAGTGAAGCCGGAGTTTGATGGTGTGAAAGAGGTCAAAAGGGACTGGGGATTGACTCTTAAAGAGGCTGCAGATGCAGTGTTTCGGGCAATAGGCAGTCGGTGGACTGTACCATGGACAGCAGAAACCATATTGCAGGTTATGCTACTCTGGGTTGTTGCTTTCTGGTTCATAGGTTCTTGGATGGTTCCGTTTGCAGCTCACCTGGCAGGTTTCAACAGGGAATCATTAACATTTAGAGGCCAGGCCTTATTCAGCCTAGTGACTGATGTAACTGAAGGTCTTGCTGGCATTATGATCCTTCACCGCTGCTTATCTCGTTTCCGTCCACTTCCTCCAGATTGGTTTAAATTTAGCTTGAAAGGAACATGGCAGATAGATGTTGTGCTAGGATGCCTCATGTTTCCCCTCGTCAATCGGCTATCACTATTCAACCTCAATCTATTACCACTCTTGCCATCCACCCCTGTCACGATATCAAGTGTTGAGCAATCAATTTTGGCACGTGATCCAGTGGCAATGGCACTGTATGCTATAGTAGTTTCAGTGTGTGCGCCCGTGTGGGAGGAGATAGTCTTTAGGGGTTTTCTACTCCCTTCATTGACCAAGTACATGCCCGTGTGGTCTGCAATTTTGGTGAGCGCCGTTGTCTTTGCTTTAGCACACTTCAATGTACAGAGGATGCTACCGCTTGTTTTCCTTGGGGTGGTGATGGGTGTTATATTTGCACGGTCAAGGAATCTATTACCATCGATGCTGTTGCACAGCCTTTGGAATGGCTTTGTATTTTTAGACTTAATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

350

Amino Acids

39.43

Weight (kDa)

9.67

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 255 - 341 1.5e-23 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60750
fragaria_vesca FvH4_5g03580
malus_domestica MD06G1095700.v1.1 MD14G1115700.v1.1
prunus_persica Prupe.5G110300_v2.0.a1 Prupe.5G110300_v2.0.a1
pyrus_communis pycom06g09200
rosa_chinensis RchiOBHm_Chr7g0196131
rosa_laevigata RLG00000004061
rosa_multiflora Rmu_sc0003017.1_g000007 Rmu_sc0003061.1_g000001
rosa_roxburghii Rroxscaffold_3G00259750
rosa_rugosa Rorug07G0030800
rosa_samantha Rh7AG156800 Rh7BG158500 Rh7CG162300 Rh7DG157600
rosa_wichuraiana Rw7G013390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 370, 443
AccB1I GGYRCC 1 cut(s) 232
AciI CCGC 3 cut(s) 37, 550, 932
AclWI GGATC 3 cut(s) 128, 535, 746
AcsI RAATTY 2 cut(s) 165, 593
AcuI CTGAAG 3 cut(s) 130, 180, 540
AcvI CACGTG 1 cut(s) 749
AdeI CACNNNGTG 1 cut(s) 232
AfaI GTAC 4 cut(s) 98, 354, 854, 918
AfiI CCNNNNNNNGG 3 cut(s) 87, 88, 949
AgsI TTSAA 4 cut(s) 460, 604, 676, 913
AjnI CCWGG 3 cut(s) 137, 447, 486
AluBI AGCT 5 cut(s) 9, 68, 212, 443, 600
AluI AGCT 5 cut(s) 9, 68, 212, 443, 600
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 3 cut(s) 128, 535, 746
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 3 cut(s) 140, 484, 489
ApeKI GCWGC 3 cut(s) 311, 440, 552
ApoI RAATTY 2 cut(s) 165, 593
AspLEI GCGC 2 cut(s) 799, 887
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 3 cut(s) 87, 88, 109
AsuHPI GGTGA 4 cut(s) 437, 539, 892, 964
AvaI CYCGRG 1 cut(s) 86
BanI GGYRCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 11
BbrPI CACGTG 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 298, 452, 539
BccI CCATC 7 cut(s) 65, 150, 257, 421, 709, 949, 1000
BceAI ACGGC 1 cut(s) 872
BcgI CGANNNNNNTGC 2 cut(s) 985, 1019
BciT130I CCWGG 3 cut(s) 139, 449, 488
BcnI CCSGG 3 cut(s) 87, 88, 109
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 3 cut(s) 153, 503, 632
BfmI CTRYAG 2 cut(s) 312, 777
BfoI RGCGCY 1 cut(s) 888
BfuAI ACCTGC 2 cut(s) 370, 443
BisI GCNGC 3 cut(s) 312, 441, 553
BlsI GCNGC 3 cut(s) 313, 442, 554
Bme1390I CCNGG 6 cut(s) 87, 88, 109, 139, 449, 488
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 3 cut(s) 75, 234, 432
BmrFI CCNGG 6 cut(s) 87, 88, 109, 139, 449, 488
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 4 cut(s) 307, 626, 915, 987
BmuI ACTGGG 1 cut(s) 298
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 69
BpuMI CCSGG 3 cut(s) 87, 88, 109
Bsa29I ATCGAT 1 cut(s) 995
BsaAI YACGTR 1 cut(s) 749
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 86, 87, 356, 943
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 88, 949
Bse1I ACTGG 2 cut(s) 293, 755
Bse3DI GCAATG 1 cut(s) 768
BseBI CCWGG 3 cut(s) 139, 449, 488
BseCI ATCGAT 1 cut(s) 995
BseDI CCNNGG 4 cut(s) 86, 87, 356, 943
BseGI GGATG 5 cut(s) 57, 432, 641, 701, 930
BseLI CCNNNNNNNGG 3 cut(s) 87, 88, 949
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 293, 755
BseRI GAGGAG 3 cut(s) 43, 200, 824
BseXI GCAGC 3 cut(s) 298, 452, 539
BshFI GGCC 3 cut(s) 142, 486, 491
BshNI GGYRCC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 995
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 4 cut(s) 87, 109, 236, 254
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 87, 88, 949
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 3 cut(s) 142, 486, 491
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 11
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 3 cut(s) 120, 540, 751
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 3 cut(s) 37, 550, 932
BspANI GGCC 3 cut(s) 142, 486, 491
BspCNI CTCAG 1 cut(s) 205
BspDI ATCGAT 1 cut(s) 995
BspLI GGNNCC 3 cut(s) 75, 234, 432
BspMAI CTGCAG 1 cut(s) 316
BspMI ACCTGC 2 cut(s) 370, 443
BspPI GGATC 3 cut(s) 128, 535, 746
BspT107I GGYRCC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 768
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 2 cut(s) 293, 755
BssECI CCNNGG 4 cut(s) 86, 87, 356, 943
BssMI GATC 3 cut(s) 120, 540, 751
BssT1I CCWWGG 2 cut(s) 356, 943
Bst2UI CCWGG 3 cut(s) 139, 449, 488
Bst4CI ACNGT 3 cut(s) 352, 771, 975
BstAPI GCANNNNNTGC 2 cut(s) 385, 773
BstAUI TGTACA 1 cut(s) 916
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 2 cut(s) 69, 213
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 5 cut(s) 57, 432, 641, 701, 930
BstH2I RGCGCY 1 cut(s) 888
BstHHI GCGC 2 cut(s) 799, 887
BstKTI GATC 3 cut(s) 123, 543, 754
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 120, 540, 751
BstMWI GCNNNNNNNGC 5 cut(s) 15, 317, 385, 449, 773
BstNI CCWGG 3 cut(s) 139, 449, 488
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 6 cut(s) 85, 86, 107, 137, 447, 486
BstSFI CTRYAG 2 cut(s) 312, 777
BstV1I GCAGC 3 cut(s) 298, 452, 539
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 995
BsuRI GGCC 3 cut(s) 142, 486, 491
BsuTUI ATCGAT 1 cut(s) 995
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 5 cut(s) 57, 432, 641, 701, 930
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 4 cut(s) 327, 762, 767, 795
BveI ACCTGC 2 cut(s) 370, 443
Cac8I GCNNGC 1 cut(s) 532
CfoI GCGC 2 cut(s) 799, 887
Cfr13I GGNCC 1 cut(s) 141
Cfr9I CCCGGG 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 995
Csp6I GTAC 4 cut(s) 97, 353, 853, 917
CviAII CATG 5 cut(s) 171, 357, 612, 643, 856
CviQI GTAC 4 cut(s) 97, 353, 853, 917
DdeI CTNAG 2 cut(s) 69, 213
DpnI GATC 3 cut(s) 122, 542, 753
DpnII GATC 3 cut(s) 120, 540, 751
DraI TTTAAA 1 cut(s) 592
DraIII CACNNNGTG 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 356, 943
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 720
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 3 cut(s) 130, 180, 540
Eco72I CACGTG 1 cut(s) 749
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 3 cut(s) 137, 447, 486
EcoRV GATATC 1 cut(s) 720
EcoT14I CCWWGG 2 cut(s) 356, 943
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 356, 943
FaeI CATG 5 cut(s) 174, 360, 615, 646, 859
FaqI GGGAC 1 cut(s) 299
FatI CATG 5 cut(s) 170, 356, 611, 642, 855
Fnu4HI GCNGC 3 cut(s) 312, 441, 553
FokI GGATG 5 cut(s) 44, 439, 648, 688, 937
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 3 cut(s) 312, 441, 553
FspBI CTAG 3 cut(s) 153, 503, 632
GlaI GCGC 2 cut(s) 798, 886
GluI GCNGC 3 cut(s) 312, 441, 553
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 888
HaeIII GGCC 3 cut(s) 142, 486, 491
HapII CCGG 4 cut(s) 87, 109, 236, 254
HhaI GCGC 2 cut(s) 799, 887
Hin1II CATG 5 cut(s) 174, 360, 615, 646, 859
Hin6I GCGC 2 cut(s) 797, 885
HinP1I GCGC 2 cut(s) 797, 885
HinfI GANTC 4 cut(s) 128, 298, 467, 982
HpaII CCGG 4 cut(s) 87, 109, 236, 254
HphI GGTGA 4 cut(s) 437, 539, 892, 964
Hpy166II GTNNAC 2 cut(s) 348, 572
Hpy188I TCNGA 3 cut(s) 78, 127, 199
Hpy188III TCNNGA 2 cut(s) 581, 715
Hpy8I GTNNAC 2 cut(s) 348, 572
HpyAV CCTTC 5 cut(s) 38, 154, 515, 554, 849
HpyCH4III ACNGT 3 cut(s) 352, 771, 975
HpyCH4IV ACGT 1 cut(s) 748
HpyCH4V TGCA 7 cut(s) 314, 320, 379, 440, 872, 971, 1006
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 317, 385, 449, 773
HpyF3I CTNAG 2 cut(s) 69, 213
HpySE526I ACGT 1 cut(s) 748
Hsp92II CATG 5 cut(s) 174, 360, 615, 646, 859
HspAI GCGC 2 cut(s) 797, 885
Kzo9I GATC 3 cut(s) 120, 540, 751
LmnI GCTCC 2 cut(s) 14, 79
Lsp1109I GCAGC 3 cut(s) 298, 452, 539
LweI GCATC 4 cut(s) 307, 626, 915, 987
MaeI CTAG 3 cut(s) 153, 503, 632
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 3 cut(s) 505, 514, 712
MalI GATC 3 cut(s) 122, 542, 753
MboI GATC 3 cut(s) 120, 540, 751
MboII GAAGA 1 cut(s) 186
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 4 cut(s) 165, 593, 738, 873
MlyI GAGTC 1 cut(s) 292
MmeI TCCRAC 1 cut(s) 101
MseI TTAA 5 cut(s) 116, 303, 473, 591, 1040
MslI CAYNNNNRTG 2 cut(s) 860, 912
MspA1I CMGCKG 1 cut(s) 552
MspI CCGG 4 cut(s) 87, 109, 236, 254
MspR9I CCNGG 6 cut(s) 87, 88, 109, 139, 449, 488
MvaI CCWGG 3 cut(s) 139, 449, 488
MwoI GCNNNNNNNGC 5 cut(s) 15, 317, 385, 449, 773
NciI CCSGG 3 cut(s) 87, 88, 109
NcoI CCATGG 1 cut(s) 356
NdeII GATC 3 cut(s) 120, 540, 751
NlaIII CATG 5 cut(s) 174, 360, 615, 646, 859
NlaIV GGNNCC 3 cut(s) 75, 234, 432
NmuCI GTSAC 2 cut(s) 505, 712
NspI RCATGY 1 cut(s) 859
PceI AGGCCT 1 cut(s) 491
PfeI GAWTC 3 cut(s) 128, 467, 982
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 3 cut(s) 313, 442, 554
PleI GAGTC 1 cut(s) 292
PmaCI CACGTG 1 cut(s) 749
PmlI CACGTG 1 cut(s) 749
PpsI GAGTC 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 3 cut(s) 137, 447, 486
PspCI CACGTG 1 cut(s) 749
PspGI CCWGG 3 cut(s) 137, 447, 486
PspN4I GGNNCC 3 cut(s) 75, 234, 432
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 4 cut(s) 98, 354, 854, 918
RsaNI GTAC 4 cut(s) 97, 353, 853, 917
RseI CAYNNNNRTG 2 cut(s) 860, 912
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 5 cut(s) 116, 303, 473, 591, 1040
SatI GCNGC 3 cut(s) 312, 441, 553
Sau3AI GATC 3 cut(s) 120, 540, 751
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 6 cut(s) 87, 88, 109, 139, 449, 488
SduI GDGCHC 1 cut(s) 11
SfaNI GCATC 4 cut(s) 307, 626, 915, 987
SfcI CTRYAG 2 cut(s) 312, 777
SmaI CCCGGG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 860, 912
Sse9I AATT 4 cut(s) 165, 593, 738, 873
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 3 cut(s) 37, 550, 932
SspMI CTAG 3 cut(s) 153, 503, 632
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 6 cut(s) 85, 86, 107, 137, 447, 486
StyI CCWWGG 2 cut(s) 356, 943
TaaI ACNGT 3 cut(s) 352, 771, 975
TaiI ACGT 1 cut(s) 751
TaqI TCGA 1 cut(s) 995
TasI AATT 4 cut(s) 165, 593, 738, 873
TatI WGTACW 2 cut(s) 852, 916
TfiI GAWTC 3 cut(s) 128, 467, 982
Tru1I TTAA 5 cut(s) 116, 303, 473, 591, 1040
Tru9I TTAA 5 cut(s) 116, 303, 473, 591, 1040
TscAI CASTG 4 cut(s) 327, 762, 774, 795
TseFI GTSAC 2 cut(s) 505, 712
TseI GCWGC 3 cut(s) 311, 440, 552
Tsp45I GTSAC 2 cut(s) 505, 712
TspDTI ATGAA 3 cut(s) 159, 403, 831
TspGWI ACGGA 2 cut(s) 423, 557
TspMI CCCGGG 1 cut(s) 86
TspRI CASTG 4 cut(s) 327, 762, 774, 795
XapI RAATTY 2 cut(s) 165, 593
XceI RCATGY 1 cut(s) 859
XmaI CCCGGG 1 cut(s) 86
XspI CTAG 3 cut(s) 153, 503, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.