FvH4_5g04531

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015621
Physical Location & Seq
Reverse (-)
2671769 .. 2672617
849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04531.t1

Sequence Viewer

Length: 348 bp
ATGTCTCACGTAACCAAGATCGTAGCCTTCTTGGTGATCGTACTCGTCTCCGAGTCCGCCATGGGTGCACCCGACTGTGTGTCAATCCTGAGGAGAATAGATCCTTGCCTTCCTTACCTGGTGCAGAACCCAGGCCGCGAGGACAGTCCATCCAAGGCTTGCTGCGACGGTGTCACAACACTGAGCATTATGCCTAACGTCAAGGATGAATGTGAGTGCATCAAGGCCTCAGTGATGTTGACTCCTGTTGATTTGGCTCGATTTGCTGCTCTCCCTAGTGTTTGCGGCACCGACTTGAAGATTCCGACCATCTCCGGCGACATGGATTGTTCACAGTTAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

116

Amino Acids

12.27

Weight (kDa)

4.72

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 13 - 100 1.3e-07 Probable lipid transfer
Tryp_alpha_amyl PF00234 26 - 110 3.1e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015621)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g04531
malus_domestica MD06G1106400.v1.1 MD06G1106500.v1.1
prunus_persica Prupe.5G118600_v2.0.a1 Prupe.5G119200_v2.0.a1 Prupe.5G119400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0194911
rosa_laevigata RLG00000004158
rosa_multiflora Rmu_ssc0000157.1_g000006
rosa_roxburghii Rroxscaffold_3G00260900
rosa_rugosa Rorug07G0018700
rosa_samantha Rh7AG146700 Rh7BG147400 Rh7CG150700 Rh7DG147900
rosa_wichuraiana Rw7G012520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 57, 136, 285
AclWI GGATC 1 cut(s) 95
AfaI GTAC 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 1 cut(s) 298
AhdI GACNNNNNGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 117, 130
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 9, 52
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 1 cut(s) 95
AoxI GGCC 2 cut(s) 133, 225
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 162, 266
AsuHPI GGTGA 1 cut(s) 46
AxyI CCTNAGG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 149, 253
BccI CCATC 2 cut(s) 157, 317
BciT130I CCWGG 2 cut(s) 119, 132
BcoDI GTCTC 2 cut(s) 9, 52
BfaI CTAG 1 cut(s) 276
BisI GCNGC 4 cut(s) 136, 163, 267, 286
BlsI GCNGC 4 cut(s) 137, 164, 268, 287
Bme1390I CCNGG 2 cut(s) 119, 132
BmeRI GACNNNNNGTC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 2 cut(s) 119, 132
BmsI GCATC 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 10
BsaJI CCNNGG 3 cut(s) 60, 130, 153
Bse21I CCTNAGG 1 cut(s) 89
BseBI CCWGG 2 cut(s) 119, 132
BseDI CCNNGG 3 cut(s) 60, 130, 153
BseGI GGATG 2 cut(s) 149, 211
BseMII CTCAG 3 cut(s) 80, 173, 243
BseRI GAGGAG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 2 cut(s) 149, 253
BsgI GTGCAG 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 135, 227
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 315
BsmAI GTCTC 2 cut(s) 9, 52
BsmBI CGTCTC 1 cut(s) 52
BsnI GGCC 2 cut(s) 135, 227
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 3 cut(s) 18, 36, 100
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 3 cut(s) 57, 136, 285
BspANI GGCC 2 cut(s) 135, 227
BspCNI CTCAG 3 cut(s) 81, 174, 242
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 289
BspPI GGATC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 287
BssECI CCNNGG 3 cut(s) 60, 130, 153
BssMI GATC 3 cut(s) 18, 36, 100
BssT1I CCWWGG 2 cut(s) 60, 153
Bst2UI CCWGG 2 cut(s) 119, 132
Bst4CI ACNGT 4 cut(s) 77, 146, 170, 336
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 89, 182, 229
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 2 cut(s) 149, 211
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 3 cut(s) 21, 39, 103
BstMAI GTCTC 2 cut(s) 9, 52
BstMBI GATC 3 cut(s) 18, 36, 100
BstMWI GCNNNNNNNGC 2 cut(s) 65, 263
BstNI CCWGG 2 cut(s) 119, 132
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 117, 130
BstSLI GKGCMC 1 cut(s) 70
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 149, 253
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu36I CCTNAGG 1 cut(s) 89
BsuRI GGCC 2 cut(s) 135, 227
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 149, 211
BtsIMutI CAGTG 2 cut(s) 179, 237
Cac8I GCNNGC 1 cut(s) 160
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 1 cut(s) 41
CviAII CATG 3 cut(s) 61, 322, 343
CviJI RGCY 5 cut(s) 26, 135, 158, 227, 257
CviKI_1 RGCY 5 cut(s) 26, 135, 158, 227, 257
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 3 cut(s) 89, 182, 229
DpnI GATC 3 cut(s) 20, 38, 102
DpnII GATC 3 cut(s) 18, 36, 100
DriI GACNNNNNGTC 1 cut(s) 79
Eam1105I GACNNNNNGTC 1 cut(s) 79
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 60, 153
Eco147I AGGCCT 1 cut(s) 227
Eco81I CCTNAGG 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 117, 130
EcoT14I CCWWGG 2 cut(s) 60, 153
ErhI CCWWGG 2 cut(s) 60, 153
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 3 cut(s) 64, 325, 346
FaiI YATR 4 cut(s) 62, 191, 323, 344
FatI CATG 3 cut(s) 60, 321, 342
Fnu4HI GCNGC 4 cut(s) 136, 163, 267, 286
FokI GGATG 2 cut(s) 136, 218
Fsp4HI GCNGC 4 cut(s) 136, 163, 267, 286
FspBI CTAG 1 cut(s) 276
GluI GCNGC 4 cut(s) 136, 163, 267, 286
HaeIII GGCC 2 cut(s) 135, 227
HapII CCGG 1 cut(s) 315
Hin1II CATG 3 cut(s) 64, 325, 346
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 3 cut(s) 53, 241, 301
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 3 cut(s) 68, 240, 332
Hpy188I TCNGA 2 cut(s) 52, 306
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 3 cut(s) 68, 240, 332
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 37, 119
HpyCH4III ACNGT 4 cut(s) 77, 146, 170, 336
HpyCH4IV ACGT 2 cut(s) 9, 198
HpyCH4V TGCA 3 cut(s) 68, 124, 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 263
HpyF3I CTNAG 3 cut(s) 89, 182, 229
HpySE526I ACGT 2 cut(s) 9, 198
Hsp92II CATG 3 cut(s) 64, 325, 346
Kzo9I GATC 3 cut(s) 18, 36, 100
LpnPI CCDG 7 cut(s) 101, 104, 117, 131, 144, 258, 328
Lsp1109I GCAGC 2 cut(s) 149, 253
LweI GCATC 1 cut(s) 228
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 1 cut(s) 276
MaeII ACGT 2 cut(s) 9, 198
MaeIII GTNAC 2 cut(s) 10, 172
MalI GATC 3 cut(s) 20, 38, 102
MboI GATC 3 cut(s) 18, 36, 100
MboII GAAGA 1 cut(s) 310
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 70
MlyI GAGTC 2 cut(s) 62, 235
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 3 cut(s) 84, 133, 238
MseI TTAA 1 cut(s) 338
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 2 cut(s) 119, 132
MvaI CCWGG 2 cut(s) 119, 132
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 65, 263
NcoI CCATGG 1 cut(s) 60
NdeII GATC 3 cut(s) 18, 36, 100
NlaIII CATG 3 cut(s) 64, 325, 346
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 346
PceI AGGCCT 1 cut(s) 227
PciI ACATGT 1 cut(s) 342
PfeI GAWTC 1 cut(s) 301
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 4 cut(s) 137, 164, 268, 287
PleI GAGTC 2 cut(s) 61, 235
PpsI GAGTC 2 cut(s) 61, 235
Ppu21I YACGTR 1 cut(s) 10
PscI ACATGT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 117, 130
PspGI CCWGG 2 cut(s) 117, 130
PspN4I GGNNCC 1 cut(s) 289
PsuI RGATCY 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 4 cut(s) 136, 163, 267, 286
Sau3AI GATC 3 cut(s) 18, 36, 100
SchI GAGTC 2 cut(s) 62, 235
ScrFI CCNGG 2 cut(s) 119, 132
SduI GDGCHC 1 cut(s) 70
SetI ASST 3 cut(s) 12, 120, 201
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 1 cut(s) 228
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 3 cut(s) 57, 136, 285
SspMI CTAG 1 cut(s) 276
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 117, 130
StyI CCWWGG 2 cut(s) 60, 153
TaaI ACNGT 4 cut(s) 77, 146, 170, 336
TaiI ACGT 2 cut(s) 12, 201
TaqI TCGA 1 cut(s) 259
TauI GCSGC 2 cut(s) 138, 288
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 2 cut(s) 186, 237
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 2 cut(s) 162, 266
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 222
TspRI CASTG 2 cut(s) 186, 237
Tth111I GACNNNGTC 1 cut(s) 170
VneI GTGCAC 1 cut(s) 66
XceI RCATGY 1 cut(s) 346
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.