RLG00000004158

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015621
Physical Location & Seq
Reverse (-)
56732799 .. 56733245
447 bp
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UTR
Exon/CDS
Intron
RLM00000004158

Sequence Viewer

Length: 348 bp
ATGTCTCACCTAACCAAGATCGTAGTCTTCTTGGTGATCGTACTCGTCTCCGAGTCCACCATGGGTGCACCCGAGTGTGCGCCAATCCTCAGGACATTAGATCCCTGCCTTCCTTACCTGGTGCAGAACCCGGGCCGCGAGGACAGCCCATCCAAGGCTTGCTGCGACGGTCTCACAGCACTCAGCGCGTTGCCGAATGTCAAGGATGAATGTGAGTGCATCAAGGCCACAGTGCTGTTGACGCCTTTAGATTTGCCTCGATTTGCTGCTCTCCCCAGTGTTTGTGGCACCACCTTGAAGATTCCGACCATCTCCGGCGACATGGACTGTTCTACGATCAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

116

Amino Acids

12.2

Weight (kDa)

4.93

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 15 - 100 1.7e-07 Probable lipid transfer
Tryp_alpha_amyl PF00234 26 - 110 2.9e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015621)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g04531
malus_domestica MD06G1106400.v1.1 MD06G1106500.v1.1
prunus_persica Prupe.5G118600_v2.0.a1 Prupe.5G119200_v2.0.a1 Prupe.5G119400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0194911
rosa_laevigata RLG00000004158
rosa_multiflora Rmu_ssc0000157.1_g000006
rosa_roxburghii Rroxscaffold_3G00260900
rosa_rugosa Rorug07G0018700
rosa_samantha Rh7AG146700 Rh7BG147400 Rh7CG150700 Rh7DG147900
rosa_wichuraiana Rw7G012520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 2 cut(s) 138, 188
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 1 cut(s) 298
AjnI CCWGG 1 cut(s) 117
AleI CACNNNNGTG 1 cut(s) 73
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 3 cut(s) 9, 52, 176
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 1 cut(s) 95
Ama87I CYCGRG 2 cut(s) 71, 130
AoxI GGCC 2 cut(s) 133, 225
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 162, 266
AspLEI GCGC 2 cut(s) 82, 188
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 2 cut(s) 131, 132
AsuHPI GGTGA 1 cut(s) 46
AvaI CYCGRG 2 cut(s) 71, 130
AxyI CCTNAGG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 149, 253
BccI CCATC 2 cut(s) 157, 317
BciT130I CCWGG 1 cut(s) 119
BcnI CCSGG 2 cut(s) 131, 132
BcoDI GTCTC 3 cut(s) 9, 52, 176
BfaI CTAG 1 cut(s) 346
BglII AGATCT 1 cut(s) 342
BisI GCNGC 3 cut(s) 136, 163, 267
BlsI GCNGC 3 cut(s) 137, 164, 268
Bme1390I CCNGG 3 cut(s) 119, 131, 132
BmeT110I CYCGRG 2 cut(s) 71, 130
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 3 cut(s) 119, 131, 132
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 19
BpuMI CCSGG 2 cut(s) 131, 132
BsaBI GATNNNNATC 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 60, 130, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 276
Bse21I CCTNAGG 1 cut(s) 89
Bse8I GATNNNNATC 1 cut(s) 341
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 3 cut(s) 60, 130, 153
BseGI GGATG 2 cut(s) 149, 211
BseJI GATNNNNATC 1 cut(s) 341
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 103, 196
BseNI ACTGG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 2 cut(s) 149, 253
BsgI GTGCAG 1 cut(s) 143
Bsh1236I CGCG 2 cut(s) 138, 188
BshFI GGCC 2 cut(s) 135, 227
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 71, 130
BsiSI CCGG 2 cut(s) 131, 315
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 3 cut(s) 9, 52, 176
BsmBI CGTCTC 1 cut(s) 52
BsnI GGCC 2 cut(s) 135, 227
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 2 cut(s) 71, 130
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 5 cut(s) 18, 36, 100, 336, 342
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 1 cut(s) 136
BspANI GGCC 2 cut(s) 135, 227
BspCNI CTCAG 2 cut(s) 102, 195
BspFNI CGCG 2 cut(s) 138, 188
BspLI GGNNCC 1 cut(s) 289
BspPI GGATC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 1 cut(s) 276
BssECI CCNNGG 3 cut(s) 60, 130, 153
BssMI GATC 5 cut(s) 18, 36, 100, 336, 342
BssNI GRCGYC 1 cut(s) 242
BssT1I CCWWGG 2 cut(s) 60, 153
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 170, 232, 329
BstACI GRCGYC 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 89, 182
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 2 cut(s) 149, 211
BstFNI CGCG 2 cut(s) 138, 188
BstHHI GCGC 2 cut(s) 82, 188
BstKTI GATC 5 cut(s) 21, 39, 103, 339, 345
BstMAI GTCTC 3 cut(s) 9, 52, 176
BstMBI GATC 5 cut(s) 18, 36, 100, 336, 342
BstMWI GCNNNNNNNGC 3 cut(s) 144, 185, 241
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 3 cut(s) 117, 129, 130
BstSLI GKGCMC 1 cut(s) 70
BstUI CGCG 2 cut(s) 138, 188
BstV1I GCAGC 2 cut(s) 149, 253
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 2 cut(s) 100, 342
BstYI RGATCY 2 cut(s) 100, 342
Bsu36I CCTNAGG 1 cut(s) 89
BsuRI GGCC 2 cut(s) 135, 227
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 149, 211
BtsIMutI CAGTG 2 cut(s) 237, 283
Cac8I GCNNGC 1 cut(s) 160
CfoI GCGC 2 cut(s) 82, 188
Cfr13I GGNCC 1 cut(s) 133
Cfr9I CCCGGG 1 cut(s) 130
CseI GACGC 1 cut(s) 250
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 1 cut(s) 41
CviAII CATG 2 cut(s) 61, 322
CviJI RGCY 4 cut(s) 135, 147, 158, 227
CviKI_1 RGCY 4 cut(s) 135, 147, 158, 227
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 2 cut(s) 89, 182
DpnI GATC 5 cut(s) 20, 38, 102, 338, 344
DpnII GATC 5 cut(s) 18, 36, 100, 336, 342
Eco130I CCWWGG 2 cut(s) 60, 153
Eco31I GGTCTC 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 89
Eco88I CYCGRG 2 cut(s) 71, 130
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 60, 153
ErhI CCWWGG 2 cut(s) 60, 153
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 2 cut(s) 64, 325
FaiI YATR 2 cut(s) 62, 323
FatI CATG 2 cut(s) 60, 321
Fnu4HI GCNGC 3 cut(s) 136, 163, 267
FokI GGATG 2 cut(s) 136, 218
Fsp4HI GCNGC 3 cut(s) 136, 163, 267
FspBI CTAG 1 cut(s) 346
GlaI GCGC 2 cut(s) 81, 187
GluI GCNGC 3 cut(s) 136, 163, 267
HaeIII GGCC 2 cut(s) 135, 227
HapII CCGG 2 cut(s) 131, 315
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 82, 188
Hin1I GRCGYC 1 cut(s) 242
Hin1II CATG 2 cut(s) 64, 325
Hin6I GCGC 2 cut(s) 80, 186
HinP1I GCGC 2 cut(s) 80, 186
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 2 cut(s) 53, 301
HpaII CCGG 2 cut(s) 131, 315
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 3 cut(s) 57, 68, 240
Hpy188I TCNGA 2 cut(s) 52, 306
Hpy188III TCNNGA 2 cut(s) 91, 340
Hpy8I GTNNAC 3 cut(s) 57, 68, 240
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 3 cut(s) 170, 232, 329
HpyCH4V TGCA 3 cut(s) 68, 124, 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 185, 241
HpyF3I CTNAG 2 cut(s) 89, 182
Hsp92I GRCGYC 1 cut(s) 242
Hsp92II CATG 2 cut(s) 64, 325
HspAI GCGC 2 cut(s) 80, 186
Kzo9I GATC 5 cut(s) 18, 36, 100, 336, 342
LpnPI CCDG 7 cut(s) 76, 104, 118, 131, 144, 289, 328
Lsp1109I GCAGC 2 cut(s) 149, 253
LweI GCATC 1 cut(s) 228
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 1 cut(s) 346
MalI GATC 5 cut(s) 20, 38, 102, 338, 344
MboI GATC 5 cut(s) 18, 36, 100, 336, 342
MboII GAAGA 2 cut(s) 19, 310
MflI RGATCY 2 cut(s) 100, 342
MhlI GDGCHC 1 cut(s) 70
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 3 cut(s) 98, 133, 267
MslI CAYNNNNRTG 1 cut(s) 73
MspI CCGG 2 cut(s) 131, 315
MspR9I CCNGG 3 cut(s) 119, 131, 132
MvaI CCWGG 1 cut(s) 119
MvnI CGCG 2 cut(s) 138, 188
MwoI GCNNNNNNNGC 3 cut(s) 144, 185, 241
NciI CCSGG 2 cut(s) 131, 132
NcoI CCATGG 1 cut(s) 60
NdeII GATC 5 cut(s) 18, 36, 100, 336, 342
NlaIII CATG 2 cut(s) 64, 325
NlaIV GGNNCC 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 73
PfeI GAWTC 1 cut(s) 301
PkrI GCNGC 3 cut(s) 137, 164, 268
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 2 cut(s) 100, 342
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 73
SatI GCNGC 3 cut(s) 136, 163, 267
Sau3AI GATC 5 cut(s) 18, 36, 100, 336, 342
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 3 cut(s) 119, 131, 132
SduI GDGCHC 1 cut(s) 70
SetI ASST 3 cut(s) 12, 120, 296
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 1 cut(s) 228
SmaI CCCGGG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 73
SsiI CCGC 1 cut(s) 136
SspMI CTAG 1 cut(s) 346
StyD4I CCNGG 3 cut(s) 117, 129, 130
StyI CCWWGG 2 cut(s) 60, 153
TaaI ACNGT 3 cut(s) 170, 232, 329
TaqI TCGA 1 cut(s) 259
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 1 cut(s) 301
TscAI CASTG 2 cut(s) 237, 283
TseI GCWGC 2 cut(s) 162, 266
TspDTI ATGAA 1 cut(s) 222
TspMI CCCGGG 1 cut(s) 130
TspRI CASTG 2 cut(s) 237, 283
VneI GTGCAC 1 cut(s) 66
XmaI CCCGGG 1 cut(s) 130
XspI CTAG 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.