FvH4_5g07800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018036
Physical Location & Seq
Forward (+)
4550342 .. 4553986
3645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07800.t1

Sequence Viewer

Length: 1392 bp
ATGGCTTCCCCAAATCTCCTTTTTCTTCTGGTTCTCTTCTCCCTTGCTACATCCGTTTCCTCCACCATAGCTTTGCCCGGAGTGGTTTACCCCACCGCTGCTTCGCCCTCTTCCACCTCTGTTTCCTCTATTGCCGCGGCTTCACCCGCCGTGGTTTCCCCCACCGTCACCATCCTCACCATACTCGGTCTGGCCAAAAGCCTCACCGACTCCCCCCACCTCTCCATGGCCCTTGGCCTCGGCCTCAGCCCACCGACGGCCACCATATATCTTCCGTCTGACACGGCTTTCCAGCACTCTGGACCTCCCTCGATACCTCTTACGCTTCGACGCCGCTACAATCTGACGATCACCTTGTCCTTTGTCGGCGACGGTTATACCTCCATCAACGCAGTCTGGGTCAACCCGACACCAGTTTTGGACTACGGGTCGGTTGTGATCTATCGGGTGGCGAAGCCGTTCCCTATCAATAGATCAGCTTCAGCGCATAAGGCTCCGGCTTCGGCGAAGCCACCTCAGCAGAAAATCCGACAAGTGGGTGGCGTTCTTCGTGCCGTCGATTGCTTCATCATGGCCGCTTTCTTCTTCAAGGCGCGAGTTGTTCCCCGACGGTTGAGCTGGGTTGATCTGGCCGGTATGCTAAACGAAAGCCATTTCCCAAATTCTGCAGATGGCAGTTGCATCACGTTGACCAAGGACGGTCAAGGAAGACCGGTTTTAATCGAAAACTTGGTGATAGTGGGACTTGAGCAAGGTTCTAAAAAATGGCTTAAGCGGCCGCCTAAGCGCCCGGAGAAAGGTTGTCATCCCGATTTTTGGCAAATCGGCGAGTGTAGGCGGCCAACCCTTTTTTTGCCGCCTGGGCGGCACCTAGGCGGCTGGTTTAATTCCGATACCGAAGAAGTACTTGAAGGATGTGGAGAAGAAGAATTGGAGGAGAAGGCTTGCAAGAATGCGAGGACCGCTCGGCTAGGGCGCGCCAGAGGAGCTTCGAATGACTTGATTCTTTGCTCTTCGACAGCCCTCACTCGAAACTCCGCCCTTTGCCCCGTGCTGTCTCCCTCCGGTTGCCCCAGGCCGATTTATCGTGGCCTTGTCTTCCTGAAGCAGCATCTGACAAAAGTTCTTATCGATTCAAATTTTGCCGATGAAGAAACCAAAGTTACCCTTCCTGCAGTGGATGAGGAAGAGCATATTGCACCTGGGACTCCTTCAGAAGAGGACGGTGAGACACTACCTGATGGTGAAACAGTACCTGTGAAAAGAAAAGGAGCAAGGAAGCAAAAGCAGAGTGGAAATGAGGAAGCTTCTAGAAAAAATGGAGCCAAGAAGTTGAAATCCAAGAAAAGCAGCAAAAGATTAAGACGGGAAAATCTTGATGAAGAAATATGA

Protein Analysis

464

Amino Acids

50.02

Weight (kDa)

9.31

Isoelectric Point (pI)

57.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018036)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07761 FvH4_5g07762 FvH4_5g07800
pyrus_communis pycom461g00020 pycom461g00340
rosa_laevigata RLG00000000445 RLG00000000453 RLG00000006086
rosa_rugosa RorugMtG0000100.1
rosa_samantha Rh4AG240000 Rh4BG243200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 867
AccBSI CCGCTC 1 cut(s) 965
AccII CGCG 3 cut(s) 137, 595, 978
AcoI YGGCCR 6 cut(s) 192, 258, 573, 630, 776, 839
AcsI RAATTY 2 cut(s) 661, 1138
AcuI CTGAAG 3 cut(s) 465, 1124, 1197
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 2 cut(s) 906, 1254
AfiI CCNNNNNNNGG 5 cut(s) 226, 256, 535, 797, 816
AflII CTTAAG 1 cut(s) 770
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 589, 911, 1137, 1336
AhdI GACNNNNNGTC 1 cut(s) 427
AjnI CCWGG 3 cut(s) 859, 1073, 1201
AluBI AGCT 5 cut(s) 71, 479, 618, 989, 1307
AluI AGCT 5 cut(s) 71, 479, 618, 989, 1307
Alw26I GTCTC 2 cut(s) 1062, 1223
AlwNI CAGNNNCTG 2 cut(s) 1114, 1256
ApeKI GCWGC 3 cut(s) 98, 1108, 1350
ApoI RAATTY 2 cut(s) 661, 1138
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
AscI GGCGCGCC 1 cut(s) 976
AsiGI ACCGGT 1 cut(s) 712
Asp700I GAANNNNTTC 1 cut(s) 458
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 5 cut(s) 487, 595, 789, 978, 980
AspS9I GGNCC 3 cut(s) 229, 302, 960
AsuC2I CCSGG 2 cut(s) 78, 791
AsuHPI GGTGA 8 cut(s) 135, 160, 169, 196, 343, 745, 1238, 1256
AsuII TTCGAA 1 cut(s) 992
AvaII GGWCC 2 cut(s) 302, 960
AvrII CCTAGG 1 cut(s) 871
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 867
BbsI GAAGAC 2 cut(s) 715, 1090
BbvCI CCTCAGC 2 cut(s) 245, 516
BbvI GCAGC 3 cut(s) 85, 1120, 1362
BccI CCATC 4 cut(s) 179, 392, 665, 1235
BceAI ACGGC 5 cut(s) 134, 273, 300, 442, 539
BciT130I CCWGG 3 cut(s) 861, 1075, 1203
BcnI CCSGG 2 cut(s) 78, 791
BcoDI GTCTC 2 cut(s) 1062, 1223
BfaI CTAG 3 cut(s) 872, 971, 1311
BfmI CTRYAG 2 cut(s) 666, 1173
BfoI RGCGCY 1 cut(s) 790
BfrI CTTAAG 1 cut(s) 770
BglI GCCNNNNNGGC 2 cut(s) 862, 865
BlnI CCTAGG 1 cut(s) 871
BmcAI AGTACT 1 cut(s) 906
Bme1390I CCNGG 5 cut(s) 78, 791, 861, 1075, 1203
Bme18I GGWCC 2 cut(s) 302, 960
BmeRI GACNNNNNGTC 1 cut(s) 427
BmgT120I GGNCC 3 cut(s) 229, 302, 960
BmiI GGNNCC 3 cut(s) 495, 869, 1324
BmrFI CCNGG 5 cut(s) 78, 791, 861, 1075, 1203
BmsI GCATC 2 cut(s) 690, 1120
BpiI GAAGAC 2 cut(s) 715, 1090
BplI GAGNNNNNCTC 2 cut(s) 949, 981
Bpu10I CCTNAGC 3 cut(s) 245, 516, 783
Bpu14I TTCGAA 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 78, 791
Bsa29I ATCGAT 1 cut(s) 1131
BsaHI GRCGYC 1 cut(s) 331
BsaWI WCCGGW 2 cut(s) 712, 1064
Bsc4I CCNNNNNNNGG 5 cut(s) 226, 256, 535, 797, 816
Bse118I RCCGGY 2 cut(s) 632, 712
Bse1I ACTGG 1 cut(s) 413
BseBI CCWGG 3 cut(s) 861, 1075, 1203
BseCI ATCGAT 1 cut(s) 1131
BseGI GGATG 5 cut(s) 50, 171, 805, 920, 1186
BseLI CCNNNNNNNGG 5 cut(s) 226, 256, 535, 797, 816
BseMII CTCAG 2 cut(s) 259, 530
BseNI ACTGG 1 cut(s) 413
BsePI GCGCGC 1 cut(s) 976
BseRI GAGGAG 2 cut(s) 950, 999
BseX3I CGGCCG 1 cut(s) 776
BseXI GCAGC 3 cut(s) 85, 1120, 1362
BseYI CCCAGC 1 cut(s) 618
Bsh1236I CGCG 3 cut(s) 137, 595, 978
Bsh1285I CGRYCG 1 cut(s) 779
BshNI GGYRCC 1 cut(s) 867
BshTI ACCGGT 1 cut(s) 712
BshVI ATCGAT 1 cut(s) 1131
BsiEI CGRYCG 1 cut(s) 779
BsiSI CCGG 6 cut(s) 78, 497, 633, 713, 791, 1065
BslFI GGGAC 2 cut(s) 756, 1219
BslI CCNNNNNNNGG 5 cut(s) 226, 256, 535, 797, 816
BsmAI GTCTC 2 cut(s) 1062, 1223
BsmFI GGGAC 2 cut(s) 756, 1219
BsmI GAATGC 1 cut(s) 958
Bsp119I TTCGAA 1 cut(s) 992
Bsp143I GATC 4 cut(s) 348, 438, 473, 625
Bsp19I CCATGG 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 258, 529
BspDI ATCGAT 1 cut(s) 1131
BspFNI CGCG 3 cut(s) 137, 595, 978
BspLI GGNNCC 3 cut(s) 495, 869, 1324
BspMAI CTGCAG 2 cut(s) 670, 1177
BspQI GCTCTTC 2 cut(s) 1018, 1182
BspT104I TTCGAA 1 cut(s) 992
BspT107I GGYRCC 1 cut(s) 867
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 965
BsrFI RCCGGY 2 cut(s) 632, 712
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 2 cut(s) 632, 712
BssHII GCGCGC 1 cut(s) 976
BssMI GATC 4 cut(s) 348, 438, 473, 625
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 4 cut(s) 225, 232, 693, 871
Bst2UI CCWGG 3 cut(s) 861, 1075, 1203
Bst4CI ACNGT 6 cut(s) 166, 374, 612, 701, 1226, 1252
Bst6I CTCTTC 5 cut(s) 41, 115, 1018, 1182, 1212
BstACI GRCGYC 1 cut(s) 331
BstAFI CTTAAG 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 992
BstC8I GCNNGC 2 cut(s) 946, 978
BstDEI CTNAG 3 cut(s) 245, 516, 783
BstDSI CCRYGG 3 cut(s) 135, 150, 225
BstF5I GGATG 5 cut(s) 50, 171, 805, 920, 1186
BstFNI CGCG 3 cut(s) 137, 595, 978
BstH2I RGCGCY 1 cut(s) 790
BstHHI GCGC 5 cut(s) 487, 595, 789, 978, 980
BstKTI GATC 4 cut(s) 351, 441, 476, 628
BstMAI GTCTC 2 cut(s) 1062, 1223
BstMBI GATC 4 cut(s) 348, 438, 473, 625
BstMCI CGRYCG 1 cut(s) 779
BstMWI GCNNNNNNNGC 9 cut(s) 146, 491, 517, 775, 784, 862, 865, 962, 986
BstNI CCWGG 3 cut(s) 861, 1075, 1203
BstSCI CCNGG 5 cut(s) 76, 789, 859, 1073, 1201
BstSFI CTRYAG 2 cut(s) 666, 1173
BstUI CGCG 3 cut(s) 137, 595, 978
BstV1I GCAGC 3 cut(s) 85, 1120, 1362
BstV2I GAAGAC 2 cut(s) 715, 1090
BstXI CCANNNNNNTGG 1 cut(s) 299
BstZI CGGCCG 1 cut(s) 776
Bsu15I ATCGAT 1 cut(s) 1131
BsuTUI ATCGAT 1 cut(s) 1131
BtgI CCRYGG 3 cut(s) 135, 150, 225
BtsCI GGATG 5 cut(s) 50, 171, 805, 920, 1186
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 1 cut(s) 1182
Cac8I GCNNGC 2 cut(s) 946, 978
CaiI CAGNNNCTG 2 cut(s) 1114, 1256
CciNI GCGGCCGC 1 cut(s) 776
CfoI GCGC 5 cut(s) 487, 595, 789, 978, 980
Cfr10I RCCGGY 2 cut(s) 632, 712
Cfr13I GGNCC 3 cut(s) 229, 302, 960
Cfr42I CCGCGG 1 cut(s) 138
ClaI ATCGAT 1 cut(s) 1131
CseI GACGC 1 cut(s) 339
Csp6I GTAC 2 cut(s) 905, 1253
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 2 cut(s) 226, 571
CviQI GTAC 2 cut(s) 905, 1253
DdeI CTNAG 3 cut(s) 245, 516, 783
DpnI GATC 4 cut(s) 350, 440, 475, 627
DpnII GATC 4 cut(s) 348, 438, 473, 625
DriI GACNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 6 cut(s) 192, 258, 573, 630, 776, 839
EagI CGGCCG 1 cut(s) 776
Eam1104I CTCTTC 5 cut(s) 41, 115, 1018, 1182, 1212
Eam1105I GACNNNNNGTC 1 cut(s) 427
EarI CTCTTC 5 cut(s) 41, 115, 1018, 1182, 1212
EciI GGCGGA 1 cut(s) 1027
EclXI CGGCCG 1 cut(s) 776
Eco130I CCWWGG 4 cut(s) 225, 232, 693, 871
Eco47I GGWCC 2 cut(s) 302, 960
Eco52I CGGCCG 1 cut(s) 776
Eco57I CTGAAG 3 cut(s) 465, 1124, 1197
EcoRII CCWGG 3 cut(s) 859, 1073, 1201
EcoT14I CCWWGG 4 cut(s) 225, 232, 693, 871
ErhI CCWWGG 4 cut(s) 225, 232, 693, 871
FaeI CATG 2 cut(s) 229, 574
FalI AAGNNNNNCTT 4 cut(s) 891, 923, 1152, 1184
FaqI GGGAC 2 cut(s) 756, 1219
FatI CATG 2 cut(s) 225, 570
FauI CCCGC 1 cut(s) 154
FokI GGATG 5 cut(s) 37, 158, 792, 927, 1193
FspBI CTAG 3 cut(s) 872, 971, 1311
GlaI GCGC 5 cut(s) 486, 594, 788, 977, 979
GsaI CCCAGC 1 cut(s) 622
HaeII RGCGCY 1 cut(s) 790
HapII CCGG 6 cut(s) 78, 497, 633, 713, 791, 1065
HgaI GACGC 1 cut(s) 339
HhaI GCGC 5 cut(s) 487, 595, 789, 978, 980
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 2 cut(s) 229, 574
Hin6I GCGC 5 cut(s) 485, 593, 787, 976, 978
HinP1I GCGC 5 cut(s) 485, 593, 787, 976, 978
HincII GTYRAC 2 cut(s) 403, 690
HindII GTYRAC 2 cut(s) 403, 690
HindIII AAGCTT 1 cut(s) 1305
HinfI GANTC 4 cut(s) 209, 1003, 1133, 1207
HpaII CCGG 6 cut(s) 78, 497, 633, 713, 791, 1065
HphI GGTGA 8 cut(s) 135, 160, 169, 196, 343, 745, 1238, 1256
Hpy166II GTNNAC 3 cut(s) 88, 403, 690
Hpy188I TCNGA 6 cut(s) 280, 345, 530, 892, 1116, 1216
Hpy188III TCNNGA 5 cut(s) 300, 809, 1102, 1311, 1376
Hpy8I GTNNAC 3 cut(s) 88, 403, 690
Hpy99I CGWCG 5 cut(s) 259, 333, 374, 560, 612
HpyAV CCTTC 4 cut(s) 905, 934, 1178, 1221
HpyCH4III ACNGT 6 cut(s) 166, 374, 612, 701, 1226, 1252
HpyCH4IV ACGT 1 cut(s) 686
HpyCH4V TGCA 5 cut(s) 668, 681, 948, 1175, 1199
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 491, 517, 775, 784, 862, 865, 962, 986
HpyF3I CTNAG 3 cut(s) 245, 516, 783
HpySE526I ACGT 1 cut(s) 686
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 2 cut(s) 229, 574
HspAI GCGC 5 cut(s) 485, 593, 787, 976, 978
KspI CCGCGG 1 cut(s) 138
Kzo9I GATC 4 cut(s) 348, 438, 473, 625
LguI GCTCTTC 2 cut(s) 1018, 1182
LmnI GCTCC 4 cut(s) 499, 986, 1271, 1322
Lsp1109I GCAGC 3 cut(s) 85, 1120, 1362
LweI GCATC 2 cut(s) 690, 1120
MaeI CTAG 3 cut(s) 872, 971, 1311
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 2 cut(s) 166, 1162
MalI GATC 4 cut(s) 350, 440, 475, 627
MbiI CCGCTC 1 cut(s) 965
MboI GATC 4 cut(s) 348, 438, 473, 625
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 4 cut(s) 661, 886, 929, 1138
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 2 cut(s) 203, 1201
MmeI TCCRAC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 458
MscI TGGCCA 1 cut(s) 194
MseI TTAA 4 cut(s) 719, 771, 885, 1361
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 98, 137
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 6 cut(s) 78, 497, 633, 713, 791, 1065
MspR9I CCNGG 5 cut(s) 78, 791, 861, 1075, 1203
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 3 cut(s) 861, 1075, 1203
MvnI CGCG 3 cut(s) 137, 595, 978
MwoI GCNNNNNNNGC 9 cut(s) 146, 491, 517, 775, 784, 862, 865, 962, 986
NciI CCSGG 2 cut(s) 78, 791
NcoI CCATGG 1 cut(s) 225
NdeII GATC 4 cut(s) 348, 438, 473, 625
NlaIII CATG 2 cut(s) 229, 574
NlaIV GGNNCC 3 cut(s) 495, 869, 1324
NmeAIII GCCGAG 2 cut(s) 219, 946
NmuCI GTSAC 1 cut(s) 166
NotI GCGGCCGC 1 cut(s) 776
NspV TTCGAA 1 cut(s) 992
PalAI GGCGCGCC 1 cut(s) 976
PauI GCGCGC 1 cut(s) 976
PciSI GCTCTTC 2 cut(s) 1018, 1182
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 2 cut(s) 1003, 1133
PinAI ACCGGT 1 cut(s) 712
PleI GAGTC 2 cut(s) 203, 1201
PpsI GAGTC 2 cut(s) 203, 1201
Psp6I CCWGG 3 cut(s) 859, 1073, 1201
PspFI CCCAGC 1 cut(s) 618
PspGI CCWGG 3 cut(s) 859, 1073, 1201
PspN4I GGNNCC 3 cut(s) 495, 869, 1324
PspPI GGNCC 3 cut(s) 229, 302, 960
PstI CTGCAG 2 cut(s) 670, 1177
PstNI CAGNNNCTG 2 cut(s) 1114, 1256
PteI GCGCGC 1 cut(s) 976
RsaI GTAC 2 cut(s) 906, 1254
RsaNI GTAC 2 cut(s) 905, 1253
SacII CCGCGG 1 cut(s) 138
SapI GCTCTTC 2 cut(s) 1018, 1182
SaqAI TTAA 4 cut(s) 719, 771, 885, 1361
Sau3AI GATC 4 cut(s) 348, 438, 473, 625
Sau96I GGNCC 3 cut(s) 229, 302, 960
ScaI AGTACT 1 cut(s) 906
SchI GAGTC 2 cut(s) 203, 1201
ScrFI CCNGG 5 cut(s) 78, 791, 861, 1075, 1203
SfaNI GCATC 2 cut(s) 690, 1120
SfcI CTRYAG 2 cut(s) 666, 1173
Sfr303I CCGCGG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 992
SgrBI CCGCGG 1 cut(s) 138
SgsI GGCGCGCC 1 cut(s) 976
SinI GGWCC 2 cut(s) 302, 960
SmlI CTYRAG 2 cut(s) 746, 770
SmoI CTYRAG 2 cut(s) 746, 770
Sse9I AATT 4 cut(s) 661, 886, 929, 1138
SspMI CTAG 3 cut(s) 872, 971, 1311
StyD4I CCNGG 5 cut(s) 76, 789, 859, 1073, 1201
StyI CCWWGG 4 cut(s) 225, 232, 693, 871
TaaI ACNGT 6 cut(s) 166, 374, 612, 701, 1226, 1252
TaiI ACGT 1 cut(s) 689
TaqI TCGA 8 cut(s) 311, 328, 558, 723, 992, 1016, 1030, 1131
TaqII GACCGA 1 cut(s) 176
TasI AATT 4 cut(s) 661, 886, 929, 1138
TatI WGTACW 1 cut(s) 904
TfiI GAWTC 2 cut(s) 1003, 1133
Tru1I TTAA 4 cut(s) 719, 771, 885, 1361
Tru9I TTAA 4 cut(s) 719, 771, 885, 1361
TscAI CASTG 1 cut(s) 1182
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 3 cut(s) 98, 1108, 1350
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 556, 1164
TspGWI ACGGA 2 cut(s) 43, 264
TspRI CASTG 1 cut(s) 1182
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 302, 960
XapI RAATTY 2 cut(s) 661, 1138
XbaI TCTAGA 1 cut(s) 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XmaJI CCTAGG 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 3 cut(s) 872, 971, 1311
ZrmI AGTACT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.