RLG00000000453

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018036
Physical Location & Seq
Reverse (-)
1021201 .. 1023903
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000453

Sequence Viewer

Length: 1395 bp
ATGGTAAGAGCTGGTAAGCATGGTAACTGTATCGGCGGCCGGGGCCGGCACTCCGCGTTCTATCTAGTTATCAAAAAGCCTACTTTACAAAGGGAACGTCGTTTCCCGGTAACCGGCCACATCGGCCCGGTAACCTTCGTCACCGTCGCTATCGAGAGAGTACAAAATGCTGACGGTGACGAAGGTTACCTACTTTCGGTGGACGCGGAGCCAACAAGTTCAGTCGAACAGCGTAACGAGTCTAAGAGTCGCGAGCTTGTTGTAGTCGGTCCTAAAGGGAGAACCTTGCTGCTTACTTGCTATATCCCCGCGTCCTTGCGCGGCTCGTTCTTCGAGCCCCGCTTCTCCCTTCCCCCTCCCCTCGTTCTACCGTCCAAAACAGGCCGTATGGAGTATAGCCAAGTGGTAAGGCATCGGTTTTTGGTACCGGCATGCAAAGGTTCGAATCCTTTTACTCCAGACTATGAACACCCGATCGGATCTGTCAAGAACGAGCTGACGACTACAGGGGAAGCGGCTGACTGCAGTTGGTTCTTCCATCAATCTCCTGGCCCTTGCTTAACTCCTTGTTCCGTAAACCGGTCGCTGGTTGATCTGATCGGACCCCGGACACGCGAGAGCCCAGCCCGGGGGGAATGCATGGTTCCCTGGCGCTTCATGGGACGGACGTTCGCAGCGAGGACCGCTCGGCTAGGGCGCGCCGGAGGAGCTTCGAGTGAGGGCTGTCGAAGAGCAAAGAATCAAGTCACTCGAAGCTCTCCCCTTTGCCCCGTGCTGTCTCCCTCCGGTTGCCCCAGGCCGAAAAACGTTGCAGTCAATTGCCCTTCCCGTTCTCATAAAAAAATAGACTACCATACGCGCCCTTTGCCGCTAAGTTCTGCACGCATTGCGGACGATAAGGTCATGCCCGTCACGAATGCAAAGTGGCGAATAAGGAACCCGGTGTCAACCCGTCACCGAGAACGGCCTATGTTCCAAGAACCAATGCTACCAATACGGTCAGCACCAACCACTGAAGTTAGCCTTCGTGATTCAGATGTTGCTGCTGCTGACATGCAAACCATTGAAGATGGTCCTCGGGGCAAAGAGCTTGAACCAGATAACAATCCCCAAGCTCCTGTTCTTCAACAACCGGAGCCTCCGAACAAGGCAAACAAATGGCAGCACCCGGGCAAGAGCGCGACTACTAGGGCACAATCCAAACTTGGAAATATATTACCAACCCCACAATTTTTATTGTTTGGAATGCAAGGGGCGTCAGCAATGGCCTCACGCGTAGGCGTCTCGACGATCTTGCTCAGACCGGGCTTTAGTCTTTGGTTGTCATCCTATCCTCTGATTAGAAGGGCCCACTATGTATCAAAATGGAAGTGGAGATTGCGTATGGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

465

Amino Acids

51.38

Weight (kDa)

10.21

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018036)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07761 FvH4_5g07762 FvH4_5g07800
pyrus_communis pycom461g00020 pycom461g00340
rosa_laevigata RLG00000000445 RLG00000000453 RLG00000006086
rosa_rugosa RorugMtG0000100.1
rosa_samantha Rh4AG240000 Rh4BG243200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 899
Acc65I GGTACC 1 cut(s) 424
AccB1I GGYRCC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 686
AclI AACGTT 1 cut(s) 807
AclWI GGATC 1 cut(s) 487
AcoI YGGCCR 2 cut(s) 37, 115
AcuI CTGAAG 1 cut(s) 1035
AcyI GRCGYC 2 cut(s) 1256, 1281
AfaI GTAC 2 cut(s) 162, 426
AfiI CCNNNNNNNGG 6 cut(s) 113, 196, 579, 586, 628, 629
AflIII ACRYGT 1 cut(s) 1273
AgeI ACCGGT 1 cut(s) 579
AgsI TTSAA 3 cut(s) 1067, 1094, 1127
AjnI CCWGG 3 cut(s) 547, 647, 794
AjuI GAANNNNNNNTTGG 2 cut(s) 1104, 1136
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
AluBI AGCT 7 cut(s) 11, 256, 496, 710, 756, 1090, 1115
AluI AGCT 7 cut(s) 11, 256, 496, 710, 756, 1090, 1115
Alw26I GTCTC 2 cut(s) 783, 1288
AlwI GGATC 1 cut(s) 487
Ama87I CYCGRG 3 cut(s) 627, 1077, 1168
ApaI GGGCCC 1 cut(s) 1351
ApeKI GCWGC 5 cut(s) 289, 674, 1043, 1046, 1162
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AscI GGCGCGCC 1 cut(s) 697
AsiGI ACCGGT 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 424
AspLEI GCGC 6 cut(s) 321, 654, 699, 701, 861, 1181
AspS9I GGNCC 9 cut(s) 43, 125, 269, 551, 602, 681, 1073, 1347, 1348
AsuHPI GGTGA 3 cut(s) 133, 188, 947
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 3 cut(s) 627, 1077, 1168
AvaII GGWCC 4 cut(s) 269, 602, 681, 1073
BaeGI GKGCMC 2 cut(s) 1195, 1351
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 3 cut(s) 339, 623, 1351
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
BbvI GCAGC 5 cut(s) 276, 686, 1030, 1033, 1174
BccI CCATC 3 cut(s) 546, 1064, 1382
BceAI ACGGC 2 cut(s) 369, 980
BcgI CGANNNNNNTGC 2 cut(s) 1276, 1310
BciT130I CCWGG 3 cut(s) 549, 649, 796
BcoDI GTCTC 2 cut(s) 783, 1288
BfaI CTAG 3 cut(s) 65, 692, 1188
BfmI CTRYAG 2 cut(s) 504, 523
BfoI RGCGCY 1 cut(s) 655
BglI GCCNNNNNGGC 1 cut(s) 123
BisI GCNGC 9 cut(s) 37, 290, 322, 516, 675, 869, 1044, 1047, 1163
BlsI GCNGC 9 cut(s) 38, 291, 323, 517, 676, 870, 1045, 1048, 1164
Bme18I GGWCC 4 cut(s) 269, 602, 681, 1073
BmeT110I CYCGRG 3 cut(s) 627, 1077, 1168
BmgT120I GGNCC 9 cut(s) 43, 125, 269, 551, 602, 681, 1073, 1347, 1348
BmiI GGNNCC 8 cut(s) 44, 210, 426, 604, 645, 938, 1137, 1349
BmsI GCATC 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpmI CTGGAG 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 1104
BsaHI GRCGYC 2 cut(s) 1256, 1281
BsaJI CCNNGG 8 cut(s) 40, 605, 627, 628, 647, 794, 1076, 1168
BsaWI WCCGGW 3 cut(s) 579, 785, 1132
BsaXI ACNNNNNCTCC 1 cut(s) 1366
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 196, 579, 586, 628, 629
Bse118I RCCGGY 4 cut(s) 45, 113, 427, 579
Bse3DI GCAATG 2 cut(s) 885, 1269
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 3 cut(s) 549, 649, 796
BseDI CCNNGG 8 cut(s) 40, 605, 627, 628, 647, 794, 1076, 1168
BseGI GGATG 2 cut(s) 1325, 1393
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 6 cut(s) 113, 196, 579, 586, 628, 629
BseMI GCAATG 2 cut(s) 885, 1269
BseMII CTCAG 1 cut(s) 1312
BsePI GCGCGC 1 cut(s) 697
BseRI GAGGAG 1 cut(s) 720
BseSI GKGCMC 2 cut(s) 1195, 1351
BseX3I CGGCCG 1 cut(s) 37
BseXI GCAGC 5 cut(s) 276, 686, 1030, 1033, 1174
BseYI CCCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 864
Bsh1285I CGRYCG 3 cut(s) 40, 477, 584
BshNI GGYRCC 1 cut(s) 424
BshTI ACCGGT 1 cut(s) 579
BsiEI CGRYCG 3 cut(s) 40, 477, 584
BsiHKCI CYCGRG 3 cut(s) 627, 1077, 1168
BslFI GGGAC 1 cut(s) 675
BslI CCNNNNNNNGG 6 cut(s) 113, 196, 579, 586, 628, 629
BsmAI GTCTC 2 cut(s) 783, 1288
BsmBI CGTCTC 1 cut(s) 1288
BsmFI GGGAC 1 cut(s) 675
BsmI GAATGC 3 cut(s) 641, 922, 1251
BsoBI CYCGRG 3 cut(s) 627, 1077, 1168
Bsp119I TTCGAA 1 cut(s) 443
Bsp120I GGGCCC 1 cut(s) 1347
Bsp1286I GDGCHC 4 cut(s) 339, 623, 1195, 1351
Bsp143I GATC 5 cut(s) 474, 479, 592, 597, 1290
Bsp68I TCGCGA 1 cut(s) 252
BspCNI CTCAG 1 cut(s) 1311
BspLI GGNNCC 8 cut(s) 44, 210, 426, 604, 645, 938, 1137, 1349
BspMAI CTGCAG 1 cut(s) 527
BspPI GGATC 1 cut(s) 487
BspQI GCTCTTC 1 cut(s) 724
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 885, 1269
BsrFI RCCGGY 4 cut(s) 45, 113, 427, 579
BssAI RCCGGY 4 cut(s) 45, 113, 427, 579
BssECI CCNNGG 8 cut(s) 40, 605, 627, 628, 647, 794, 1076, 1168
BssHII GCGCGC 1 cut(s) 697
BssMI GATC 5 cut(s) 474, 479, 592, 597, 1290
BssNI GRCGYC 2 cut(s) 1256, 1281
Bst2UI CCWGG 3 cut(s) 549, 649, 796
Bst4CI ACNGT 5 cut(s) 29, 145, 176, 372, 999
Bst6I CTCTTC 1 cut(s) 724
BstACI GRCGYC 2 cut(s) 1256, 1281
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 5 cut(s) 47, 254, 433, 699, 883
BstDEI CTNAG 3 cut(s) 243, 872, 1298
BstEII GGTNACC 3 cut(s) 109, 130, 185
BstF5I GGATG 2 cut(s) 1325, 1393
BstH2I RGCGCY 1 cut(s) 655
BstHHI GCGC 6 cut(s) 321, 654, 699, 701, 861, 1181
BstKTI GATC 5 cut(s) 477, 482, 595, 600, 1293
BstMAI GTCTC 2 cut(s) 783, 1288
BstMBI GATC 5 cut(s) 474, 479, 592, 597, 1290
BstMCI CGRYCG 3 cut(s) 40, 477, 584
BstMWI GCNNNNNNNGC 6 cut(s) 42, 123, 683, 707, 865, 887
BstNI CCWGG 3 cut(s) 549, 649, 796
BstNSI RCATGY 2 cut(s) 435, 1057
BstPI GGTNACC 3 cut(s) 109, 130, 185
BstSFI CTRYAG 2 cut(s) 504, 523
BstSLI GKGCMC 2 cut(s) 1195, 1351
BstV1I GCAGC 5 cut(s) 276, 686, 1030, 1033, 1174
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BstZI CGGCCG 1 cut(s) 37
BtsCI GGATG 2 cut(s) 1325, 1393
BtsIMutI CAGTG 1 cut(s) 1011
BtuMI TCGCGA 1 cut(s) 252
Cac8I GCNNGC 5 cut(s) 47, 254, 433, 699, 883
CfoI GCGC 6 cut(s) 321, 654, 699, 701, 861, 1181
Cfr10I RCCGGY 4 cut(s) 45, 113, 427, 579
Cfr13I GGNCC 9 cut(s) 43, 125, 269, 551, 602, 681, 1073, 1347, 1348
Cfr9I CCCGGG 2 cut(s) 627, 1168
CseI GACGC 4 cut(s) 212, 300, 1245, 1270
Csp6I GTAC 2 cut(s) 161, 425
CspAI ACCGGT 1 cut(s) 579
CviAII CATG 6 cut(s) 20, 432, 640, 658, 904, 1054
CviQI GTAC 2 cut(s) 161, 425
DdeI CTNAG 3 cut(s) 243, 872, 1298
DpnI GATC 5 cut(s) 476, 481, 594, 599, 1292
DpnII GATC 5 cut(s) 474, 479, 592, 597, 1290
DrdI GACNNNNNNGTC 1 cut(s) 899
DseDI GACNNNNNNGTC 1 cut(s) 899
EaeI YGGCCR 2 cut(s) 37, 115
EagI CGGCCG 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 724
EclXI CGGCCG 1 cut(s) 37
Eco24I GRGCYC 3 cut(s) 339, 623, 1351
Eco47I GGWCC 4 cut(s) 269, 602, 681, 1073
Eco52I CGGCCG 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 1035
Eco88I CYCGRG 3 cut(s) 627, 1077, 1168
Eco91I GGTNACC 3 cut(s) 109, 130, 185
EcoO109I RGGNCCY 1 cut(s) 1347
EcoO65I GGTNACC 3 cut(s) 109, 130, 185
EcoRII CCWGG 3 cut(s) 547, 647, 794
EcoT22I ATGCAT 1 cut(s) 641
EcoT38I GRGCYC 3 cut(s) 339, 623, 1351
Esp3I CGTCTC 1 cut(s) 1288
FaeI CATG 6 cut(s) 23, 435, 643, 661, 907, 1057
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FaqI GGGAC 1 cut(s) 675
FatI CATG 6 cut(s) 19, 431, 639, 657, 903, 1053
FauI CCCGC 2 cut(s) 316, 347
Fnu4HI GCNGC 9 cut(s) 37, 290, 322, 516, 675, 869, 1044, 1047, 1163
FokI GGATG 1 cut(s) 1312
FriOI GRGCYC 3 cut(s) 339, 623, 1351
Fsp4HI GCNGC 9 cut(s) 37, 290, 322, 516, 675, 869, 1044, 1047, 1163
FspBI CTAG 3 cut(s) 65, 692, 1188
GlaI GCGC 6 cut(s) 320, 653, 698, 700, 860, 1180
GluI GCNGC 9 cut(s) 37, 290, 322, 516, 675, 869, 1044, 1047, 1163
GsaI CCCAGC 1 cut(s) 626
GsuI CTGGAG 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 655
HgaI GACGC 4 cut(s) 212, 300, 1245, 1270
HhaI GCGC 6 cut(s) 321, 654, 699, 701, 861, 1181
Hin1I GRCGYC 2 cut(s) 1256, 1281
Hin1II CATG 6 cut(s) 23, 435, 643, 661, 907, 1057
Hin6I GCGC 6 cut(s) 319, 652, 697, 699, 859, 1179
HinP1I GCGC 6 cut(s) 319, 652, 697, 699, 859, 1179
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HinfI GANTC 5 cut(s) 239, 247, 445, 739, 1031
HphI GGTGA 3 cut(s) 133, 188, 947
Hpy166II GTNNAC 3 cut(s) 202, 577, 948
Hpy188I TCNGA 7 cut(s) 479, 597, 602, 1036, 1143, 1301, 1338
Hpy188III TCNNGA 7 cut(s) 154, 251, 458, 487, 913, 1028, 1285
Hpy8I GTNNAC 3 cut(s) 202, 577, 948
Hpy99I CGWCG 3 cut(s) 102, 149, 1291
HpyAV CCTTC 6 cut(s) 145, 176, 359, 834, 1034, 1338
HpyCH4III ACNGT 5 cut(s) 29, 145, 176, 372, 999
HpyCH4IV ACGT 3 cut(s) 97, 668, 807
HpyCH4V TGCA 8 cut(s) 435, 525, 639, 812, 881, 920, 1057, 1249
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 123, 683, 707, 865, 887
HpyF3I CTNAG 3 cut(s) 243, 872, 1298
HpySE526I ACGT 3 cut(s) 97, 668, 807
Hsp92I GRCGYC 2 cut(s) 1256, 1281
Hsp92II CATG 6 cut(s) 23, 435, 643, 661, 907, 1057
HspAI GCGC 6 cut(s) 319, 652, 697, 699, 859, 1179
KpnI GGTACC 1 cut(s) 428
KroI GCCGGC 1 cut(s) 45
KroNI GCCGGC 1 cut(s) 47
Kzo9I GATC 5 cut(s) 474, 479, 592, 597, 1290
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 4 cut(s) 208, 707, 1120, 1135
Lsp1109I GCAGC 5 cut(s) 276, 686, 1030, 1033, 1174
LweI GCATC 1 cut(s) 421
MaeI CTAG 3 cut(s) 65, 692, 1188
MaeII ACGT 3 cut(s) 97, 668, 807
MalI GATC 5 cut(s) 476, 481, 594, 599, 1292
MbiI CCGCTC 1 cut(s) 686
MboI GATC 5 cut(s) 474, 479, 592, 597, 1290
MboII GAAGA 5 cut(s) 322, 526, 741, 1079, 1115
MfeI CAATTG 1 cut(s) 817
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 4 cut(s) 339, 623, 1195, 1351
MluCI AATT 2 cut(s) 817, 1229
MluI ACGCGT 1 cut(s) 1273
MlyI GAGTC 2 cut(s) 248, 256
Mph1103I ATGCAT 1 cut(s) 641
MroNI GCCGGC 1 cut(s) 45
MseI TTAA 1 cut(s) 560
MunI CAATTG 1 cut(s) 817
Mva1269I GAATGC 3 cut(s) 641, 922, 1251
MvaI CCWGG 3 cut(s) 549, 649, 796
MwoI GCNNNNNNNGC 6 cut(s) 42, 123, 683, 707, 865, 887
NaeI GCCGGC 1 cut(s) 47
NdeII GATC 5 cut(s) 474, 479, 592, 597, 1290
NgoMIV GCCGGC 1 cut(s) 45
NlaIII CATG 6 cut(s) 23, 435, 643, 661, 907, 1057
NlaIV GGNNCC 8 cut(s) 44, 210, 426, 604, 645, 938, 1137, 1349
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 5 cut(s) 139, 176, 745, 910, 953
NruI TCGCGA 1 cut(s) 252
NsiI ATGCAT 1 cut(s) 641
NspI RCATGY 2 cut(s) 435, 1057
NspV TTCGAA 1 cut(s) 443
PaeI GCATGC 1 cut(s) 435
PalAI GGCGCGCC 1 cut(s) 697
PauI GCGCGC 1 cut(s) 697
PciSI GCTCTTC 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 674
PctI GAATGC 3 cut(s) 641, 922, 1251
PdiI GCCGGC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 445, 739, 1031
PinAI ACCGGT 1 cut(s) 579
PkrI GCNGC 9 cut(s) 38, 291, 323, 517, 676, 870, 1045, 1048, 1164
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 2 cut(s) 247, 255
PpsI GAGTC 2 cut(s) 247, 255
Psp1406I AACGTT 1 cut(s) 807
Psp6I CCWGG 3 cut(s) 547, 647, 794
PspEI GGTNACC 3 cut(s) 109, 130, 185
PspFI CCCAGC 1 cut(s) 622
PspGI CCWGG 3 cut(s) 547, 647, 794
PspN4I GGNNCC 8 cut(s) 44, 210, 426, 604, 645, 938, 1137, 1349
PspOMI GGGCCC 1 cut(s) 1347
PspPI GGNCC 9 cut(s) 43, 125, 269, 551, 602, 681, 1073, 1347, 1348
PsrI GAACNNNNNNTAC 2 cut(s) 972, 1004
PstI CTGCAG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 479
PteI GCGCGC 1 cut(s) 697
PvuI CGATCG 1 cut(s) 477
RruI TCGCGA 1 cut(s) 252
RsaI GTAC 2 cut(s) 162, 426
RsaNI GTAC 2 cut(s) 161, 425
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 9 cut(s) 37, 290, 322, 516, 675, 869, 1044, 1047, 1163
Sau3AI GATC 5 cut(s) 474, 479, 592, 597, 1290
Sau96I GGNCC 9 cut(s) 43, 125, 269, 551, 602, 681, 1073, 1347, 1348
SchI GAGTC 2 cut(s) 248, 256
SduI GDGCHC 4 cut(s) 339, 623, 1195, 1351
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 2 cut(s) 504, 523
SfiI GGCCNNNNNGGCC 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 443
SgsI GGCGCGCC 1 cut(s) 697
SinI GGWCC 4 cut(s) 269, 602, 681, 1073
SmaI CCCGGG 2 cut(s) 629, 1170
SphI GCATGC 1 cut(s) 435
Sse9I AATT 2 cut(s) 817, 1229
SspMI CTAG 3 cut(s) 65, 692, 1188
TaaI ACNGT 5 cut(s) 29, 145, 176, 372, 999
TaiI ACGT 3 cut(s) 100, 671, 810
TaqI TCGA 8 cut(s) 153, 225, 333, 443, 713, 727, 751, 1286
TaqII GACCGA 1 cut(s) 257
TasI AATT 2 cut(s) 817, 1229
TatI WGTACW 1 cut(s) 160
TauI GCSGC 4 cut(s) 39, 324, 518, 871
TfiI GAWTC 3 cut(s) 445, 739, 1031
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 5 cut(s) 139, 176, 745, 910, 953
TseI GCWGC 5 cut(s) 289, 674, 1043, 1046, 1162
Tsp45I GTSAC 5 cut(s) 139, 176, 745, 910, 953
TspDTI ATGAA 2 cut(s) 480, 646
TspGWI ACGGA 2 cut(s) 562, 679
TspMI CCCGGG 2 cut(s) 627, 1168
TspRI CASTG 1 cut(s) 1018
VpaK11BI GGWCC 4 cut(s) 269, 602, 681, 1073
XceI RCATGY 2 cut(s) 435, 1057
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmaI CCCGGG 2 cut(s) 627, 1168
XspI CTAG 3 cut(s) 65, 692, 1188
Zsp2I ATGCAT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.