FvH4_5g07890
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005997
Physical Location & Seq
Forward (+)
4584677 .. 4588899
4223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07890.t2

Sequence Viewer

Length: 2244 bp
ATGTTCTTGATGGGGTGTTGTAGATCATTTCTGCTACTTCTTTTTGCATTGAGTTCTTTATTTTTGGGTACTCACCAACTACCATCTTCTCAAACCCAAGTGCTTCTACAGCTAAGGAAGCATTTGGAGTATCCAAGCCAGTTGGAAATTTGGAAGGATCATACTGTAGATTTCTGCTCTATATCTTCATCTGCACAAGTAAACATCACATGCTTGGACAACCTTGTAGTTGAGCTCAGAATCAAGGGTGATAAGCCAGCTAAAGTTACTGGCTTTCATGGGTTTTCAATTCCAGGCCAGACTTTATCAGAGGCCTTCTTATTGGATTCTTTTGTTACTAATCTGGTAAGACTAAGCAGCTTGAAAGTCCTCAGTCTTGTCTCTTTGGGAATCTGGGGTCAACTTCCAGACAAAATTCACAGGTTGACTTCCCTTGAGTATCTAGATTTGAGTTCGAACTTTCTCTTTGGTTCAGTCCCACCTCGAATTTCTGCAATGGTGAAGCTTCAAACTCTTGCAATGGATGACAATTTCTTGAATGGCACTGTTCCCAGTTGGTTTGGTTCACTATCCAATCTTTCAACTCTAAGCTTTAGAAATAACCAGTTGCGAGGTCCATTACTAGATCTTAGTAGCTTAGGTAGCTTACAAGTACTTGATTTGAGTGTGAACAAGTTGAATGATGTATTACCCTCAATGCCAAAACGGTTGGTCATGCTTTCCCTCAGCAACAATGCCTTCTCTGGTGAGATTCCTCAGCAATATCGAAAATTAAGCGCGCTTCAACACCTTGACATGTCATTCAACACACTTACAGGCACACCTCTTGCTGCATTGTTCTCATTGCCATACATCAGCTACTTGAATTTGGCTTCGAATTCGTTAAGTGGTTCGCTTCCAAGCCATTTAAGCTGTGGCATTAAACTTGATTACATTGATATATCAAACAATAGCTTGACAGGTGACTTGCCTTCTTGTTTGGGAACTGAATCAGCACAGAGAGTTGTTAAGTTTGGTGGGAATTGCTTATCTGTTAGTATGCAAAATCAGCATCCACAGTCATATTGCAATGTAGTAGTCAGTCCAAAGGAGAAGCAATCTGGAGGAAAAGAAGTAGGAATCTTGGTGGGTATGATTGCTGGAATATTAGTGCTTTTAGTGCTTCTGGTTTTGAGCTGTATCCTTTTATGTAGAAGATATCTCCCCAGGGGAGTATCTGAGCAGCATTTGCTGCATAAATCAGAGCAAGATCACCCAGTAGCAGTAGGGTTCTCCACTGAGATGCTAACAAATGCAAGGTTCATTTCACAAGCAGCAAAGGCAGGCATGCTAGGTCTCCCAGGATGTCGAACGTTTTCATTAGAGGAGTTGGTGGATATTACCAGAAATTTTGACAACTCTGCCTTGTTGGGTGAAGGTTCATACGGGAAGCTTTACAAAGGTAGACTACAGAATGGGATCCAAGTTGTTATTAGATGCCTGCTTGTGTCAAAGAAATACACAATTCGAAATCTTAAACTGCGGTTGGATTTGCTTGCCAAGCTTCGTCACCCGCATTTAGTATGCCTTTTAGGACACTGCCTTGACGGTGGTGGAGATGATTACAGTGTGAACAAGGTGTACCTTGTCTCTGAGTATGTGTCAAATGGAACCTTTCGTGCTAAACTCTCTGGTAGGCCTGAAAAAGTTCTCAACTGGCCACGGAGATTGGGAGTCCTCATAGGTGTAGCCAAGGCTGTGCACTTCCTACATACTGGAATTATTCCAGGTTTCTTGAGCAACCGGCTGAAGATGAACAACATCTTGCTCAATGAGCATGATAATGCAAAATTAAGTGACTACGGACTGTCTATTATCTCAGAAGAAACTGATACGTCTATGGCAAATGAAGGCCACAAGTCATGGCAAATGAAAAGTTTAGAGGATGATGCTTATAGCTTCGGATATATATTGCTCGAGGCTATTGTTGGAGCTTCAGTCTCTGCTAGAAGAGAGGCATTCCTGCTAAATGATTTGGCATCTCTGAACACCCAGGAAGGCCGAAAGCAGCTAGTAGAACCAATAGTCCTGGCAACTTGTTCACAACAATCTTTATCAATTGTGATCTCCATAATGAGCAAATGCATTTCTCCAGAAGCAAGCCGTCCATCTTTTGAAGACATTCTTTGGAACTTACAATATGCAGTTCAAGTACAGGCAACAGCTGACGGCGACAATAGATATGATACTGCATCACATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

748

Amino Acids

82.15

Weight (kDa)

8.46

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 112 - 227 2.9e-10 Leucine-rich repeat region
LRR_8 PF13855 168 - 226 6.3e-06 Leucine rich repeat
LRR_8 PF13855 236 - 295 3e-06 Leucine rich repeat
Pkinase PF00069 466 - 721 2.3e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 468 - 724 5e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1444
AccII CGCG 1 cut(s) 779
AciI CCGC 2 cut(s) 1522, 1553
AclI AACGTT 1 cut(s) 1352
AclWI GGATC 3 cut(s) 165, 1453, 1466
AcoI YGGCCR 1 cut(s) 1697
AcsI RAATTY 6 cut(s) 147, 414, 486, 865, 877, 1387
AcuI CTGAAG 2 cut(s) 1808, 1959
AfaI GTAC 4 cut(s) 70, 654, 1622, 2193
AflIII ACRYGT 1 cut(s) 795
AjnI CCWGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
AloI GAACNNNNNNTCC 2 cut(s) 2049, 2081
Alw21I GWGCWC 2 cut(s) 237, 1743
Alw26I GTCTC 4 cut(s) 385, 1340, 1633, 1984
Alw44I GTGCAC 1 cut(s) 1739
AlwI GGATC 3 cut(s) 165, 1453, 1466
AlwNI CAGNNNCTG 1 cut(s) 1982
Ama87I CYCGRG 1 cut(s) 1955
AoxI GGCC 6 cut(s) 295, 312, 1676, 1697, 1891, 2038
ApaLI GTGCAC 1 cut(s) 1739
ApeKI GCWGC 6 cut(s) 357, 830, 1222, 1231, 1313, 2047
ApoI RAATTY 6 cut(s) 147, 414, 486, 865, 877, 1387
Asp700I GAANNNNTTC 3 cut(s) 1354, 1686, 2160
AspLEI GCGC 2 cut(s) 779, 781
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 8 cut(s) 65, 260, 511, 758, 974, 1244, 1424, 1541
AsuII TTCGAA 3 cut(s) 455, 875, 1507
AvaI CYCGRG 1 cut(s) 1955
AvaII GGWCC 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 1743
BalI TGGCCA 1 cut(s) 1699
BamHI GGATCC 1 cut(s) 1458
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 2163
Bbv12I GWGCWC 2 cut(s) 237, 1743
BbvCI CCTCAGC 2 cut(s) 725, 756
BbvI GCAGC 6 cut(s) 369, 817, 1218, 1234, 1325, 2059
BccI CCATC 3 cut(s) 4, 91, 2155
BceAI ACGGC 2 cut(s) 2127, 2224
BciT130I CCWGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
BciVI GTATCC 2 cut(s) 141, 1190
BcoDI GTCTC 4 cut(s) 385, 1340, 1633, 1984
BfaI CTAG 5 cut(s) 443, 623, 1331, 1986, 2051
BfmI CTRYAG 3 cut(s) 107, 165, 1448
BfuI GTATCC 2 cut(s) 141, 1190
BglII AGATCT 1 cut(s) 625
BisI GCNGC 6 cut(s) 358, 831, 1223, 1232, 1314, 2048
BlsI GCNGC 6 cut(s) 359, 832, 1224, 1233, 1315, 2049
BmcAI AGTACT 1 cut(s) 654
Bme1390I CCNGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 1 cut(s) 1955
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 2 cut(s) 1460, 1651
BmrFI CCNGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
BmrI ACTGGG 2 cut(s) 546, 1250
BmsI GCATC 6 cut(s) 1060, 1272, 1466, 1918, 2027, 2240
BmuI ACTGGG 2 cut(s) 546, 1250
BpiI GAAGAC 1 cut(s) 2163
BpmI CTGGAG 2 cut(s) 1122, 2115
Bpu10I CCTNAGC 4 cut(s) 113, 637, 725, 756
Bpu14I TTCGAA 3 cut(s) 455, 875, 1507
BpuEI CTTGAG 2 cut(s) 455, 1795
BsaI GGTCTC 1 cut(s) 1340
BsaJI CCNNGG 6 cut(s) 1205, 1206, 1339, 1700, 1731, 2031
BsaXI ACNNNNNCTCC 2 cut(s) 1962, 1992
Bse118I RCCGGY 1 cut(s) 1782
Bse1I ACTGG 7 cut(s) 139, 274, 552, 604, 1256, 1700, 1759
Bse3DI GCAATG 4 cut(s) 501, 525, 842, 1075
BseBI CCWGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
BseDI CCNNGG 6 cut(s) 1205, 1206, 1339, 1700, 1731, 2031
BseGI GGATG 4 cut(s) 529, 1051, 1349, 1930
BseMI GCAATG 4 cut(s) 501, 525, 842, 1075
BseMII CTCAG 8 cut(s) 250, 385, 739, 770, 1209, 1269, 1623, 1872
BseNI ACTGG 7 cut(s) 139, 274, 552, 604, 1256, 1700, 1759
BsePI GCGCGC 1 cut(s) 777
BseRI GAGGAG 1 cut(s) 1379
BseSI GKGCMC 1 cut(s) 1743
BseXI GCAGC 6 cut(s) 369, 817, 1218, 1234, 1325, 2059
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 6 cut(s) 297, 314, 1678, 1699, 1893, 2040
BsiHKAI GWGCWC 2 cut(s) 237, 1743
BsiHKCI CYCGRG 1 cut(s) 1955
BsiSI CCGG 1 cut(s) 1783
BslFI GGGAC 1 cut(s) 461
BsmAI GTCTC 4 cut(s) 385, 1340, 1633, 1984
BsmFI GGGAC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 1997
BsnI GGCC 6 cut(s) 297, 314, 1678, 1699, 1893, 2040
Bso31I GGTCTC 1 cut(s) 1340
BsoBI CYCGRG 1 cut(s) 1955
Bsp119I TTCGAA 3 cut(s) 455, 875, 1507
Bsp1286I GDGCHC 2 cut(s) 237, 1743
Bsp143I GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
BspACI CCGC 2 cut(s) 1522, 1553
BspANI GGCC 6 cut(s) 297, 314, 1678, 1699, 1893, 2040
BspCNI CTCAG 8 cut(s) 249, 384, 738, 769, 1210, 1270, 1624, 1871
BspFNI CGCG 1 cut(s) 779
BspLI GGNNCC 2 cut(s) 1460, 1651
BspPI GGATC 3 cut(s) 165, 1453, 1466
BspT104I TTCGAA 3 cut(s) 455, 875, 1507
BspTNI GGTCTC 1 cut(s) 1340
BsrDI GCAATG 4 cut(s) 501, 525, 842, 1075
BsrFI RCCGGY 1 cut(s) 1782
BsrI ACTGG 7 cut(s) 139, 274, 552, 604, 1256, 1700, 1759
BssAI RCCGGY 1 cut(s) 1782
BssECI CCNNGG 6 cut(s) 1205, 1206, 1339, 1700, 1731, 2031
BssHII GCGCGC 1 cut(s) 777
BssMI GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
BssT1I CCWWGG 1 cut(s) 1731
Bst2UI CCWGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
Bst4CI ACNGT 7 cut(s) 166, 547, 708, 1059, 1589, 1607, 1848
Bst6I CTCTTC 1 cut(s) 1984
BstAPI GCANNNNNTGC 2 cut(s) 1228, 1231
BstBI TTCGAA 3 cut(s) 455, 875, 1507
BstC8I GCNNGC 7 cut(s) 258, 779, 1324, 1328, 1481, 1536, 2140
BstDSI CCRYGG 1 cut(s) 1700
BstF5I GGATG 4 cut(s) 529, 1051, 1349, 1930
BstFNI CGCG 1 cut(s) 779
BstHHI GCGC 2 cut(s) 779, 781
BstKTI GATC 6 cut(s) 26, 160, 628, 1252, 1461, 2106
BstMAI GTCTC 4 cut(s) 385, 1340, 1633, 1984
BstMBI GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
BstNI CCWGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
BstNSI RCATGY 3 cut(s) 213, 799, 1330
BstSCI CCNGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
BstSFI CTRYAG 3 cut(s) 107, 165, 1448
BstSLI GKGCMC 1 cut(s) 1743
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 6 cut(s) 369, 817, 1218, 1234, 1325, 2059
BstV2I GAAGAC 1 cut(s) 2163
BstX2I RGATCY 2 cut(s) 625, 1458
BstYI RGATCY 2 cut(s) 625, 1458
BsuI GTATCC 2 cut(s) 141, 1190
BsuRI GGCC 6 cut(s) 297, 314, 1678, 1699, 1893, 2040
BtgI CCRYGG 1 cut(s) 1700
BtsCI GGATG 4 cut(s) 529, 1051, 1349, 1930
BtsI GCAGTG 1 cut(s) 1576
BtsIMutI CAGTG 4 cut(s) 543, 1275, 1576, 1612
Cac8I GCNNGC 7 cut(s) 258, 779, 1324, 1328, 1481, 1536, 2140
CaiI CAGNNNCTG 1 cut(s) 1982
CfoI GCGC 2 cut(s) 779, 781
Cfr10I RCCGGY 1 cut(s) 1782
Cfr13I GGNCC 1 cut(s) 614
Csp6I GTAC 4 cut(s) 69, 653, 1621, 2192
CspCI CAANNNNNGTGG 2 cut(s) 1689, 1724
CviAII CATG 7 cut(s) 210, 278, 715, 796, 1327, 1817, 1902
CviQI GTAC 4 cut(s) 69, 653, 1621, 2192
DpnI GATC 6 cut(s) 25, 159, 627, 1251, 1460, 2105
DpnII GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
EaeI YGGCCR 1 cut(s) 1697
Eam1104I CTCTTC 1 cut(s) 1984
EarI CTCTTC 1 cut(s) 1984
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 1731
Eco147I AGGCCT 2 cut(s) 314, 1678
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 1340
Eco32I GATATC 1 cut(s) 1199
Eco47I GGWCC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 1808, 1959
Eco88I CYCGRG 1 cut(s) 1955
EcoICRI GAGCTC 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 877
EcoRII CCWGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
EcoRV GATATC 1 cut(s) 1199
EcoT14I CCWWGG 1 cut(s) 1731
EcoT22I ATGCAT 1 cut(s) 2126
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 1731
FaeI CATG 7 cut(s) 213, 281, 718, 799, 1330, 1820, 1905
FalI AAGNNNNNCTT 2 cut(s) 2148, 2180
FaqI GGGAC 1 cut(s) 461
FatI CATG 7 cut(s) 209, 277, 714, 795, 1326, 1816, 1901
FauI CCCGC 1 cut(s) 1560
FblI GTMKAC 1 cut(s) 1444
Fnu4HI GCNGC 6 cut(s) 358, 831, 1223, 1232, 1314, 2048
FokI GGATG 4 cut(s) 536, 1038, 1356, 1937
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 6 cut(s) 358, 831, 1223, 1232, 1314, 2048
FspBI CTAG 5 cut(s) 443, 623, 1331, 1986, 2051
GlaI GCGC 2 cut(s) 778, 780
GluI GCNGC 6 cut(s) 358, 831, 1223, 1232, 1314, 2048
GsuI CTGGAG 2 cut(s) 1122, 2115
HaeIII GGCC 6 cut(s) 297, 314, 1678, 1699, 1893, 2040
HapII CCGG 1 cut(s) 1783
HhaI GCGC 2 cut(s) 779, 781
Hin1II CATG 7 cut(s) 213, 281, 718, 799, 1330, 1820, 1905
Hin6I GCGC 2 cut(s) 777, 779
HinP1I GCGC 2 cut(s) 777, 779
HincII GTYRAC 2 cut(s) 401, 426
HindII GTYRAC 2 cut(s) 401, 426
HindIII AAGCTT 4 cut(s) 503, 589, 1430, 1541
HinfI GANTC 7 cut(s) 240, 326, 390, 751, 989, 1119, 1713
HpaII CCGG 1 cut(s) 1783
HphI GGTGA 8 cut(s) 65, 260, 511, 758, 974, 1244, 1424, 1541
Hpy188I TCNGA 8 cut(s) 239, 310, 1219, 1243, 1633, 1861, 1943, 2025
Hpy188III TCNNGA 7 cut(s) 7, 407, 443, 535, 1101, 1774, 2132
HpyAV CCTTC 7 cut(s) 148, 325, 748, 981, 1409, 1883, 2030
HpyCH4III ACNGT 7 cut(s) 166, 547, 708, 1059, 1589, 1607, 1848
HpyCH4IV ACGT 2 cut(s) 1352, 1874
HpySE526I ACGT 2 cut(s) 1352, 1874
Hsp92II CATG 7 cut(s) 213, 281, 718, 799, 1330, 1820, 1905
HspAI GCGC 2 cut(s) 777, 779
Kzo9I GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
LmnI GCTCC 1 cut(s) 1970
Lsp1109I GCAGC 6 cut(s) 369, 817, 1218, 1234, 1325, 2059
LweI GCATC 6 cut(s) 1060, 1272, 1466, 1918, 2027, 2240
MaeI CTAG 5 cut(s) 443, 623, 1331, 1986, 2051
MaeII ACGT 2 cut(s) 1352, 1874
MaeIII GTNAC 5 cut(s) 265, 334, 962, 1547, 1835
MalI GATC 6 cut(s) 25, 159, 627, 1251, 1460, 2105
MboI GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
MboII GAAGA 7 cut(s) 78, 177, 1206, 1801, 1874, 2001, 2168
MfeI CAATTG 1 cut(s) 2097
MflI RGATCY 2 cut(s) 625, 1458
MhlI GDGCHC 2 cut(s) 237, 1743
MlsI TGGCCA 1 cut(s) 1699
MluNI TGGCCA 1 cut(s) 1699
MlyI GAGTC 1 cut(s) 1722
MmeI TCCRAC 3 cut(s) 123, 1506, 1948
Mox20I TGGCCA 1 cut(s) 1699
Mph1103I ATGCAT 1 cut(s) 2126
MroXI GAANNNNTTC 3 cut(s) 1354, 1686, 2160
MscI TGGCCA 1 cut(s) 1699
MseI TTAA 7 cut(s) 773, 884, 908, 921, 1008, 1515, 1832
MslI CAYNNNNRTG 2 cut(s) 1280, 1821
Msp20I TGGCCA 1 cut(s) 1699
MspA1I CMGCKG 1 cut(s) 2204
MspI CCGG 1 cut(s) 1783
MspR9I CCNGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
MunI CAATTG 1 cut(s) 2097
Mva1269I GAATGC 1 cut(s) 1997
MvaI CCWGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
MvnI CGCG 1 cut(s) 779
NdeII GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
NlaIII CATG 7 cut(s) 213, 281, 718, 799, 1330, 1820, 1905
NlaIV GGNNCC 2 cut(s) 1460, 1651
NmuCI GTSAC 3 cut(s) 962, 1547, 1835
NsiI ATGCAT 1 cut(s) 2126
NspI RCATGY 3 cut(s) 213, 799, 1330
NspV TTCGAA 3 cut(s) 455, 875, 1507
PaeI GCATGC 1 cut(s) 1330
PaeR7I CTCGAG 1 cut(s) 1955
PasI CCCWGGG 1 cut(s) 1206
PauI GCGCGC 1 cut(s) 777
PceI AGGCCT 2 cut(s) 314, 1678
PciI ACATGT 1 cut(s) 795
PctI GAATGC 1 cut(s) 1997
PdmI GAANNNNTTC 3 cut(s) 1354, 1686, 2160
PfeI GAWTC 6 cut(s) 240, 326, 390, 751, 989, 1119
PkrI GCNGC 6 cut(s) 359, 832, 1224, 1233, 1315, 2049
PleI GAGTC 1 cut(s) 1721
PpsI GAGTC 1 cut(s) 1721
PscI ACATGT 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 1352
Psp6I CCWGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
PspGI CCWGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
PspN4I GGNNCC 2 cut(s) 1460, 1651
PspPI GGNCC 1 cut(s) 614
PspXI VCTCGAGB 1 cut(s) 1955
PsrI GAACNNNNNNTAC 2 cut(s) 1604, 1636
PstNI CAGNNNCTG 1 cut(s) 1982
PsuI RGATCY 2 cut(s) 625, 1458
PteI GCGCGC 1 cut(s) 777
PvuII CAGCTG 1 cut(s) 2204
RsaI GTAC 4 cut(s) 70, 654, 1622, 2193
RsaNI GTAC 4 cut(s) 69, 653, 1621, 2192
RseI CAYNNNNRTG 2 cut(s) 1280, 1821
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 7 cut(s) 773, 884, 908, 921, 1008, 1515, 1832
SatI GCNGC 6 cut(s) 358, 831, 1223, 1232, 1314, 2048
Sau3AI GATC 6 cut(s) 23, 157, 625, 1249, 1458, 2103
Sau96I GGNCC 1 cut(s) 614
ScaI AGTACT 1 cut(s) 654
SchI GAGTC 1 cut(s) 1722
ScrFI CCNGG 6 cut(s) 294, 1207, 1341, 1767, 2033, 2069
SduI GDGCHC 2 cut(s) 237, 1743
SfaNI GCATC 6 cut(s) 1060, 1272, 1466, 1918, 2027, 2240
SfcI CTRYAG 3 cut(s) 107, 165, 1448
Sfr274I CTCGAG 1 cut(s) 1955
SfuI TTCGAA 3 cut(s) 455, 875, 1507
SinI GGWCC 1 cut(s) 614
SlaI CTCGAG 1 cut(s) 1955
SmiMI CAYNNNNRTG 2 cut(s) 1280, 1821
SmlI CTYRAG 3 cut(s) 434, 1774, 1955
SmoI CTYRAG 3 cut(s) 434, 1774, 1955
SphI GCATGC 1 cut(s) 1330
SseBI AGGCCT 2 cut(s) 314, 1678
SsiI CCGC 2 cut(s) 1522, 1553
SspI AATATT 1 cut(s) 1146
SspMI CTAG 5 cut(s) 443, 623, 1331, 1986, 2051
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 2 cut(s) 314, 1678
StyD4I CCNGG 6 cut(s) 292, 1205, 1339, 1765, 2031, 2067
StyI CCWWGG 1 cut(s) 1731
TaaI ACNGT 7 cut(s) 166, 547, 708, 1059, 1589, 1607, 1848
TaiI ACGT 2 cut(s) 1355, 1877
TaqI TCGA 7 cut(s) 455, 484, 766, 875, 1348, 1507, 1956
TatI WGTACW 2 cut(s) 652, 2191
TfiI GAWTC 6 cut(s) 240, 326, 390, 751, 989, 1119
Tru1I TTAA 7 cut(s) 773, 884, 908, 921, 1008, 1515, 1832
Tru9I TTAA 7 cut(s) 773, 884, 908, 921, 1008, 1515, 1832
TscAI CASTG 4 cut(s) 550, 1282, 1583, 1612
TseFI GTSAC 3 cut(s) 962, 1547, 1835
TseI GCWGC 6 cut(s) 357, 830, 1222, 1231, 1313, 2047
Tsp45I GTSAC 3 cut(s) 962, 1547, 1835
TspDTI ATGAA 8 cut(s) 177, 266, 1291, 1347, 1410, 1808, 1902, 1925
TspGWI ACGGA 2 cut(s) 1717, 1857
TspRI CASTG 4 cut(s) 550, 1282, 1583, 1612
VneI GTGCAC 1 cut(s) 1739
VpaK11BI GGWCC 1 cut(s) 614
XapI RAATTY 6 cut(s) 147, 414, 486, 865, 877, 1387
XbaI TCTAGA 1 cut(s) 442
XceI RCATGY 3 cut(s) 213, 799, 1330
XcmI CCANNNNNNNNNTGG 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 1955
XmiI GTMKAC 1 cut(s) 1444
XmnI GAANNNNTTC 3 cut(s) 1354, 1686, 2160
XspI CTAG 5 cut(s) 443, 623, 1331, 1986, 2051
ZrmI AGTACT 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 2126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.