MD06G1145000.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005997
Physical Location & Seq
Reverse (-)
28785311 .. 28788301
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1145000.v1.1.491

Sequence Viewer

Length: 2235 bp
ATGGGGCTTTGTGAATCGTTTCTGCTATTCTGTTTTTCTTGGAGCTTCTTACTTCTAGGCACTCATCAACTCCAATCTTCTCAAACCCAAGTGCTTCTGCAGCTCAGGAAGCACTTAGAGTACCCAAAACAGCTAGAAATTTGGAACAGTCACAACACAGATTTCTGCTCCATTTCTTCACTTGGCCAAGTAAACATTACATGCTTGGACAATCTTCTAATTGTGCTCAGAATCAAGGGTGATAAGATTAAGCAATCCAAAAAAAGTCATTACTTCAATGGCTTTTCAATCCAAAGCCAAACTTTATCAGATTCCTTTTCTTTGGATTCTTTTGTCACTACCCTTTCAAGGCTAAACAGTTTGAGAGTTCTTAGCCTTGTGTCTTTGGGCATCTGGGGTCAACTTCCAGACAAAGTTCATAGGTTGTCTTCCCTTGAGTATCTCGATTTGAGCTCGAACTTCCTCTTCGGTTCAATCCCACCAAGGATTTCCGCAATGGCGATGCTTCAAACACTTAAAATCGATGGGAATTTCTTAAATGATACTATCCCTGATTGGTTCAATTCGCTATCCAATCTTACAACTCTGAGCTTGAGGAACAACAAGTTGGAAGGTGCATTGCAGGATTTTAGTAGGTTAAGCAGTTTACAAGTGCTAGATTTGAGTGGGAACAGACTGAGCGCTATGTTGCCTCCAATGCCAAAAGGGTTGGTCATGCTTTTTGTCAGCGAAAACTCCTTCTCCGGCAAGATTCCAGTGAAATATGGCAAGTTAAGTGGACTTCAACACATTGATATGTCACACAATGCACTTATAGGCACACCTCCTGCTGCACTCTTCTCTTTGCCTAATATTAGTTACTTGAACTTGGGGTCAAATCTGTTGAGTGGCTCGCTTCCAGGGAATCTAATCTGTGGAAGCAAACTTGATTTCATTGATATATCGAATAATAGTTTGACGGGTGATTTGCCTTCATGTTTAAGAAATGAATCAAAAGAGAGAGTTGTGAAGTTTGGTGGGAATTGTTTGTCCATTGGTGTGCAGCATCAGCAGCATGAACAATCACACTGCAAGGAAGTGATCAGCCTGAAGAAGAAAGAGTTCAGAGGGAACAATGTAGGGATCTTGGTGGGTGTGATTCTAGGATTAATTGTACTTACTGTGCTTTTGGTTTTAGGGTTTATCATTTTCTGCAGAAGATATTGCCCTCAGGGGATATCAAAGCGGCATTTGCTGCAGAAATCAGTGCAAGAGAATTCAGCAGTTGGGTTTTCGACCAAGCTTCTAACAAGTGCAAGGTTTATTTCACAAGCTGGAAAGGTAGGGACACAAGGCCTCCCTGCGTGCCGGATATTTTCTATGGAAGAGCTGATGGAAGCTACGAAAAACTTCGACAGCTCTGCCTTATTGGGTGAAGGTTCATATGGGAGGCTATACAAAGGAAGACTATACAATGGGACTGAAGTTGCCATAAGGTGCCTGCCCTTGTTGAAGAAGTACACAATTCGAAATGTTAAACTCCGGTTGGAACTGCTTTCCAAGCTTCGGCACCAGCATTTGGTATGCCTTTTAGGGCACTGTCTCGATGGTGGTGGACAAGATGATTACAGTCCGAACAAGGTCTATTTTGTGTCTGAATATGTGCCCAGTGGGACATTCCGTGATCATCTCTCTGCGAATTGTCCTGGAAAGGTTCTCAACTGGTCAGAGAGATTGGCAGTTCTAGTTAGCGTGGCCAAGGCCGTGCGCTTCCTACATACGGGAATCATTCCTGGTTTCTTCTGCAACCGCCTGAAGACGAACAATATCTTGCTCAATGAGCACGGTATGGCAAAATTGAGTGACTATGGACTGTCCATTCTCACAGAAGAGACCGATAAATCCACAGCAAAAGACAGCCTTACATCATGGCAAATGACAAGTTTAGACGATGATGCTTATAGCTTTGGATACATACTACTCGAGGCTCTCGTTGGATCTTCCATATCTTCTACAAGAGAAGCAATCCTGCAAAATAATATGGCATCTCTGAGTAGTGAGGACGGGCGGAAGCAGATAGTTGAACCGGCTATTCTAGCCAGTTGCTCACAAGAGTCTCTATCGATCGTGCTCTCCGTAATGTACAAATGCATTTGTCCAGAGTCGAGCCGTCCATCTTTCGAGGACATCCTTTGGAACTTACAGTATGCAGTTCAAGTCCAGGAAGAAACAGCAGATGGAGACCAAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

745

Amino Acids

82.34

Weight (kDa)

8.56

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 112 - 222 6.4e-10 Leucine-rich repeat region
LRR_8 PF13855 169 - 226 1.1e-11 Leucine rich repeat
LRR_4 PF12799 192 - 227 4.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 260 - 319 5.2e-06 Leucine rich repeat
Pkinase PF00069 467 - 724 8.9e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 468 - 726 4e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1476, 1548
AccB7I CCANNNNNTGG 1 cut(s) 1558
AciI CCGC 4 cut(s) 492, 1225, 1789, 2047
AclWI GGATC 2 cut(s) 1130, 1984
AcoI YGGCCR 2 cut(s) 184, 1734
AcsI RAATTY 3 cut(s) 138, 529, 1255
AcuI CTGAAG 3 cut(s) 1109, 1482, 1814
AfaI GTAC 4 cut(s) 122, 1155, 1499, 2123
AfeI AGCGCT 1 cut(s) 682
AfiI CCNNNNNNNGG 4 cut(s) 348, 1545, 1558, 1759
AjnI CCWGG 4 cut(s) 898, 1684, 1771, 2199
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
Alw21I GWGCWC 4 cut(s) 228, 455, 1824, 2112
Alw26I GTCTC 4 cut(s) 1586, 1865, 2100, 2214
AlwI GGATC 2 cut(s) 1130, 1984
Ama87I CYCGRG 1 cut(s) 1961
Aor51HI AGCGCT 1 cut(s) 682
AoxI GGCC 4 cut(s) 184, 1333, 1734, 1740
ApeKI GCWGC 5 cut(s) 100, 830, 1042, 1051, 1234
ApoI RAATTY 3 cut(s) 138, 529, 1255
ArsI GACNNNNNNTTYG 2 cut(s) 949, 981
AseI ATTAAT 1 cut(s) 1148
Asp700I GAANNNNTTC 3 cut(s) 18, 1100, 1388
AspLEI GCGC 2 cut(s) 683, 1749
AsuHPI GGTGA 3 cut(s) 251, 974, 1424
AsuII TTCGAA 1 cut(s) 1507
AvaI CYCGRG 1 cut(s) 1961
AxyI CCTNAGG 1 cut(s) 1209
BaeGI GKGCMC 2 cut(s) 1578, 1647
BalI TGGCCA 2 cut(s) 186, 1736
BanI GGYRCC 2 cut(s) 1476, 1548
BanII GRGCYC 1 cut(s) 455
BbsI GAAGAC 3 cut(s) 420, 1450, 1802
Bbv12I GWGCWC 4 cut(s) 228, 455, 1824, 2112
BbvI GCAGC 5 cut(s) 112, 817, 1054, 1063, 1221
BccI CCATC 5 cut(s) 518, 1366, 1580, 2161, 2210
BceAI ACGGC 2 cut(s) 1727, 2133
BcgI CGANNNNNNTGC 2 cut(s) 1382, 1416
BciT130I CCWGG 4 cut(s) 900, 1686, 1773, 2201
BciVI GTATCC 1 cut(s) 1943
BclI TGATCA 2 cut(s) 1080, 1663
BcoDI GTCTC 4 cut(s) 1586, 1865, 2100, 2214
BfaI CTAG 6 cut(s) 56, 134, 656, 1142, 1724, 2075
BfmI CTRYAG 3 cut(s) 98, 1192, 1235
BfoI RGCGCY 1 cut(s) 684
BfuI GTATCC 1 cut(s) 1943
BisI GCNGC 6 cut(s) 101, 831, 1043, 1052, 1226, 1235
BlsI GCNGC 6 cut(s) 102, 832, 1044, 1053, 1227, 1236
Bme1390I CCNGG 4 cut(s) 900, 1686, 1773, 2201
BmeT110I CYCGRG 1 cut(s) 1961
BmiI GGNNCC 2 cut(s) 1478, 1550
BmrFI CCNGG 4 cut(s) 900, 1686, 1773, 2201
BmrI ACTGGG 1 cut(s) 1641
BmsI GCATC 5 cut(s) 399, 492, 1054, 1924, 2033
BmuI ACTGGG 1 cut(s) 1641
BpiI GAAGAC 3 cut(s) 420, 1450, 1802
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 1507
BpuEI CTTGAG 2 cut(s) 455, 613
Bsa29I ATCGAT 2 cut(s) 522, 2102
BsaI GGTCTC 2 cut(s) 1865, 2214
BsaJI CCNNGG 3 cut(s) 482, 899, 1737
BsaWI WCCGGW 1 cut(s) 1521
BsaXI ACNNNNNCTCC 4 cut(s) 725, 755, 1320, 1350
Bsc4I CCNNNNNNNGG 4 cut(s) 348, 1545, 1558, 1759
Bse118I RCCGGY 1 cut(s) 2065
Bse1I ACTGG 4 cut(s) 755, 1647, 1706, 2079
Bse21I CCTNAGG 1 cut(s) 1209
Bse3DI GCAATG 2 cut(s) 501, 617
BseBI CCWGG 4 cut(s) 900, 1686, 1773, 2201
BseCI ATCGAT 2 cut(s) 522, 2102
BseDI CCNNGG 3 cut(s) 482, 899, 1737
BseGI GGATG 1 cut(s) 2166
BseLI CCNNNNNNNGG 4 cut(s) 348, 1545, 1558, 1759
BseMI GCAATG 2 cut(s) 501, 617
BseMII CTCAG 6 cut(s) 118, 241, 578, 668, 1223, 2021
BseNI ACTGG 4 cut(s) 755, 1647, 1706, 2079
BseSI GKGCMC 2 cut(s) 1578, 1647
BseXI GCAGC 5 cut(s) 112, 817, 1054, 1063, 1221
BsgI GTGCAG 2 cut(s) 816, 1061
Bsh1285I CGRYCG 1 cut(s) 2106
BshFI GGCC 4 cut(s) 186, 1335, 1736, 1742
BshNI GGYRCC 2 cut(s) 1476, 1548
BshVI ATCGAT 2 cut(s) 522, 2102
BsiEI CGRYCG 1 cut(s) 2106
BsiHKAI GWGCWC 4 cut(s) 228, 455, 1824, 2112
BsiHKCI CYCGRG 1 cut(s) 1961
BsiSI CCGG 4 cut(s) 744, 1348, 1522, 2066
BslFI GGGAC 3 cut(s) 1339, 1471, 1666
BslI CCNNNNNNNGG 4 cut(s) 348, 1545, 1558, 1759
BsmAI GTCTC 4 cut(s) 1586, 1865, 2100, 2214
BsmFI GGGAC 3 cut(s) 1339, 1471, 1666
BsnI GGCC 4 cut(s) 186, 1335, 1736, 1742
Bso31I GGTCTC 2 cut(s) 1865, 2214
BsoBI CYCGRG 1 cut(s) 1961
Bsp119I TTCGAA 1 cut(s) 1507
Bsp1286I GDGCHC 6 cut(s) 228, 455, 1578, 1647, 1824, 2112
Bsp1407I TGTACA 1 cut(s) 2121
Bsp143I GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
BspACI CCGC 4 cut(s) 492, 1225, 1789, 2047
BspANI GGCC 4 cut(s) 186, 1335, 1736, 1742
BspCNI CTCAG 6 cut(s) 117, 240, 579, 669, 1222, 2022
BspDI ATCGAT 2 cut(s) 522, 2102
BspLI GGNNCC 2 cut(s) 1478, 1550
BspMAI CTGCAG 3 cut(s) 102, 1196, 1239
BspPI GGATC 2 cut(s) 1130, 1984
BspQI GCTCTTC 1 cut(s) 1359
BspT104I TTCGAA 1 cut(s) 1507
BspT107I GGYRCC 2 cut(s) 1476, 1548
BspTNI GGTCTC 2 cut(s) 1865, 2214
BsrDI GCAATG 2 cut(s) 501, 617
BsrFI RCCGGY 1 cut(s) 2065
BsrGI TGTACA 1 cut(s) 2121
BsrI ACTGG 4 cut(s) 755, 1647, 1706, 2079
BssAI RCCGGY 1 cut(s) 2065
BssECI CCNNGG 3 cut(s) 482, 899, 1737
BssMI GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
BssT1I CCWWGG 2 cut(s) 482, 1737
Bst2UI CCWGG 4 cut(s) 900, 1686, 1773, 2201
Bst4CI ACNGT 8 cut(s) 149, 359, 1162, 1580, 1610, 1826, 1854, 2184
Bst6I CTCTTC 4 cut(s) 470, 842, 1359, 1863
BstAPI GCANNNNNTGC 1 cut(s) 1234
BstAUI TGTACA 1 cut(s) 2121
BstBI TTCGAA 1 cut(s) 1507
BstC8I GCNNGC 3 cut(s) 893, 1345, 1481
BstDEI CTNAG 8 cut(s) 104, 115, 227, 371, 587, 677, 1209, 2030
BstF5I GGATG 1 cut(s) 2166
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 2 cut(s) 683, 1749
BstKTI GATC 5 cut(s) 1083, 1125, 1666, 1979, 2106
BstMAI GTCTC 4 cut(s) 1586, 1865, 2100, 2214
BstMBI GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
BstMCI CGRYCG 1 cut(s) 2106
BstNI CCWGG 4 cut(s) 900, 1686, 1773, 2201
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 4 cut(s) 898, 1684, 1771, 2199
BstSFI CTRYAG 3 cut(s) 98, 1192, 1235
BstSLI GKGCMC 2 cut(s) 1578, 1647
BstV1I GCAGC 5 cut(s) 112, 817, 1054, 1063, 1221
BstV2I GAAGAC 3 cut(s) 420, 1450, 1802
BstX2I RGATCY 2 cut(s) 1122, 1976
BstYI RGATCY 2 cut(s) 1122, 1976
Bsu15I ATCGAT 2 cut(s) 522, 2102
Bsu36I CCTNAGG 1 cut(s) 1209
BsuI GTATCC 1 cut(s) 1943
BsuRI GGCC 4 cut(s) 186, 1335, 1736, 1742
BsuTUI ATCGAT 2 cut(s) 522, 2102
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 1 cut(s) 2166
BtsI GCAGTG 1 cut(s) 1066
BtsIMutI CAGTG 5 cut(s) 762, 1066, 1251, 1576, 1654
Cac8I GCNNGC 3 cut(s) 893, 1345, 1481
CfoI GCGC 2 cut(s) 683, 1749
Cfr10I RCCGGY 1 cut(s) 2065
ClaI ATCGAT 2 cut(s) 522, 2102
Csp6I GTAC 4 cut(s) 121, 1154, 1498, 2122
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 5 cut(s) 201, 715, 975, 1055, 1908
CviQI GTAC 4 cut(s) 121, 1154, 1498, 2122
DdeI CTNAG 8 cut(s) 104, 115, 227, 371, 587, 677, 1209, 2030
DpnI GATC 5 cut(s) 1082, 1124, 1665, 1978, 2105
DpnII GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
EaeI YGGCCR 2 cut(s) 184, 1734
Eam1104I CTCTTC 4 cut(s) 470, 842, 1359, 1863
EarI CTCTTC 4 cut(s) 470, 842, 1359, 1863
EciI GGCGGA 1 cut(s) 2062
Ecl136II GAGCTC 1 cut(s) 453
Eco130I CCWWGG 2 cut(s) 482, 1737
Eco147I AGGCCT 1 cut(s) 1335
Eco24I GRGCYC 1 cut(s) 455
Eco31I GGTCTC 2 cut(s) 1865, 2214
Eco32I GATATC 1 cut(s) 1218
Eco47III AGCGCT 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 453
Eco57I CTGAAG 3 cut(s) 1109, 1482, 1814
Eco81I CCTNAGG 1 cut(s) 1209
Eco88I CYCGRG 1 cut(s) 1961
EcoICRI GAGCTC 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 1255
EcoRII CCWGG 4 cut(s) 898, 1684, 1771, 2199
EcoRV GATATC 1 cut(s) 1218
EcoT14I CCWWGG 2 cut(s) 482, 1737
EcoT22I ATGCAT 1 cut(s) 2132
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 2 cut(s) 482, 1737
FaeI CATG 5 cut(s) 204, 718, 978, 1058, 1911
FalI AAGNNNNNCTT 4 cut(s) 286, 318, 1884, 1916
FaqI GGGAC 3 cut(s) 1339, 1471, 1666
FatI CATG 5 cut(s) 200, 714, 974, 1054, 1907
FauNDI CATATG 1 cut(s) 1423
FbaI TGATCA 2 cut(s) 1080, 1663
Fnu4HI GCNGC 6 cut(s) 101, 831, 1043, 1052, 1226, 1235
FokI GGATG 1 cut(s) 2153
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 6 cut(s) 101, 831, 1043, 1052, 1226, 1235
FspBI CTAG 6 cut(s) 56, 134, 656, 1142, 1724, 2075
GlaI GCGC 2 cut(s) 682, 1748
GluI GCNGC 6 cut(s) 101, 831, 1043, 1052, 1226, 1235
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 4 cut(s) 186, 1335, 1736, 1742
HapII CCGG 4 cut(s) 744, 1348, 1522, 2066
HhaI GCGC 2 cut(s) 683, 1749
Hin1II CATG 5 cut(s) 204, 718, 978, 1058, 1911
Hin6I GCGC 2 cut(s) 681, 1747
HinP1I GCGC 2 cut(s) 681, 1747
HincII GTYRAC 1 cut(s) 401
HindII GTYRAC 1 cut(s) 401
HindIII AAGCTT 2 cut(s) 1280, 1541
HpaII CCGG 4 cut(s) 744, 1348, 1522, 2066
HphI GGTGA 3 cut(s) 251, 974, 1424
Hpy166II GTNNAC 6 cut(s) 193, 401, 647, 779, 1500, 1595
Hpy188I TCNGA 8 cut(s) 230, 310, 588, 1106, 1614, 1636, 1708, 2031
Hpy188III TCNNGA 5 cut(s) 106, 407, 443, 1583, 2138
Hpy8I GTNNAC 6 cut(s) 193, 401, 647, 779, 1500, 1595
HpyAV CCTTC 4 cut(s) 605, 748, 981, 1409
HpyCH4III ACNGT 8 cut(s) 149, 359, 1162, 1580, 1610, 1826, 1854, 2184
HpyF3I CTNAG 8 cut(s) 104, 115, 227, 371, 587, 677, 1209, 2030
Hsp92II CATG 5 cut(s) 204, 718, 978, 1058, 1911
HspAI GCGC 2 cut(s) 681, 1747
Ksp22I TGATCA 2 cut(s) 1080, 1663
Kzo9I GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
LguI GCTCTTC 1 cut(s) 1359
LmnI GCTCC 2 cut(s) 42, 173
Lsp1109I GCAGC 5 cut(s) 112, 817, 1054, 1063, 1221
LweI GCATC 5 cut(s) 399, 492, 1054, 1924, 2033
MaeI CTAG 6 cut(s) 56, 134, 656, 1142, 1724, 2075
MaeIII GTNAC 5 cut(s) 149, 334, 798, 857, 1841
MalI GATC 5 cut(s) 1082, 1124, 1665, 1978, 2105
MboI GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
MflI RGATCY 2 cut(s) 1122, 1976
MhlI GDGCHC 6 cut(s) 228, 455, 1578, 1647, 1824, 2112
MlsI TGGCCA 2 cut(s) 186, 1736
MluNI TGGCCA 2 cut(s) 186, 1736
MlyI GAGTC 2 cut(s) 2102, 2150
MmeI TCCRAC 3 cut(s) 588, 1506, 1954
Mox20I TGGCCA 2 cut(s) 186, 1736
Mph1103I ATGCAT 1 cut(s) 2132
MroXI GAANNNNTTC 3 cut(s) 18, 1100, 1388
MscI TGGCCA 2 cut(s) 186, 1736
MseI TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1148, 1515
MslI CAYNNNNRTG 2 cut(s) 794, 1037
Msp20I TGGCCA 2 cut(s) 186, 1736
MspI CCGG 4 cut(s) 744, 1348, 1522, 2066
MspR9I CCNGG 4 cut(s) 900, 1686, 1773, 2201
MvaI CCWGG 4 cut(s) 900, 1686, 1773, 2201
NdeI CATATG 1 cut(s) 1423
NdeII GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
NlaIII CATG 5 cut(s) 204, 718, 978, 1058, 1911
NlaIV GGNNCC 2 cut(s) 1478, 1550
NmuCI GTSAC 4 cut(s) 149, 334, 798, 1841
NsiI ATGCAT 1 cut(s) 2132
NspI RCATGY 1 cut(s) 204
NspV TTCGAA 1 cut(s) 1507
PaeR7I CTCGAG 1 cut(s) 1961
PceI AGGCCT 1 cut(s) 1335
PciSI GCTCTTC 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 2 cut(s) 1968, 2112
PdmI GAANNNNTTC 3 cut(s) 18, 1100, 1388
PfeI GAWTC 9 cut(s) 14, 231, 311, 326, 751, 904, 989, 1138, 1764
PflMI CCANNNNNTGG 1 cut(s) 1558
PfoI TCCNGGA 2 cut(s) 1684, 2199
PkrI GCNGC 6 cut(s) 102, 832, 1044, 1053, 1227, 1236
Ple19I CGATCG 1 cut(s) 2106
PleI GAGTC 2 cut(s) 2101, 2149
PpsI GAGTC 2 cut(s) 2101, 2149
PshBI ATTAAT 1 cut(s) 1148
Psp124BI GAGCTC 1 cut(s) 455
Psp6I CCWGG 4 cut(s) 898, 1684, 1771, 2199
PspGI CCWGG 4 cut(s) 898, 1684, 1771, 2199
PspN4I GGNNCC 2 cut(s) 1478, 1550
PspXI VCTCGAGB 1 cut(s) 1961
PstI CTGCAG 3 cut(s) 102, 1196, 1239
PsuI RGATCY 2 cut(s) 1122, 1976
PvuI CGATCG 1 cut(s) 2106
RsaI GTAC 4 cut(s) 122, 1155, 1499, 2123
RsaNI GTAC 4 cut(s) 121, 1154, 1498, 2122
RseI CAYNNNNRTG 2 cut(s) 794, 1037
SacI GAGCTC 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 1359
SaqAI TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1148, 1515
SatI GCNGC 6 cut(s) 101, 831, 1043, 1052, 1226, 1235
Sau3AI GATC 5 cut(s) 1080, 1122, 1663, 1976, 2103
SchI GAGTC 2 cut(s) 2102, 2150
ScrFI CCNGG 4 cut(s) 900, 1686, 1773, 2201
SduI GDGCHC 6 cut(s) 228, 455, 1578, 1647, 1824, 2112
SfaNI GCATC 5 cut(s) 399, 492, 1054, 1924, 2033
SfcI CTRYAG 3 cut(s) 98, 1192, 1235
Sfr274I CTCGAG 1 cut(s) 1961
SfuI TTCGAA 1 cut(s) 1507
SlaI CTCGAG 1 cut(s) 1961
SmiMI CAYNNNNRTG 2 cut(s) 794, 1037
SmlI CTYRAG 3 cut(s) 434, 592, 1961
SmoI CTYRAG 3 cut(s) 434, 592, 1961
SseBI AGGCCT 1 cut(s) 1335
SsiI CCGC 4 cut(s) 492, 1225, 1789, 2047
SspI AATATT 1 cut(s) 853
SspMI CTAG 6 cut(s) 56, 134, 656, 1142, 1724, 2075
SstI GAGCTC 1 cut(s) 455
StuI AGGCCT 1 cut(s) 1335
StyD4I CCNGG 4 cut(s) 898, 1684, 1771, 2199
StyI CCWWGG 2 cut(s) 482, 1737
TaaI ACNGT 8 cut(s) 149, 359, 1162, 1580, 1610, 1826, 1854, 2184
TaqII GACCGA 1 cut(s) 1889
TatI WGTACW 3 cut(s) 1153, 1497, 2121
TauI GCSGC 1 cut(s) 1228
TfiI GAWTC 9 cut(s) 14, 231, 311, 326, 751, 904, 989, 1138, 1764
Tru1I TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1148, 1515
Tru9I TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1148, 1515
TscAI CASTG 5 cut(s) 762, 1073, 1251, 1583, 1654
TseFI GTSAC 4 cut(s) 149, 334, 798, 1841
TseI GCWGC 5 cut(s) 100, 830, 1042, 1051, 1234
Tsp45I GTSAC 4 cut(s) 149, 334, 798, 1841
TspDTI ATGAA 6 cut(s) 407, 922, 963, 1002, 1071, 1410
TspGWI ACGGA 2 cut(s) 1649, 2104
TspRI CASTG 5 cut(s) 762, 1073, 1251, 1583, 1654
Van91I CCANNNNNTGG 1 cut(s) 1558
VspI ATTAAT 1 cut(s) 1148
XapI RAATTY 3 cut(s) 138, 529, 1255
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 1961
XmnI GAANNNNTTC 3 cut(s) 18, 1100, 1388
XspI CTAG 6 cut(s) 56, 134, 656, 1142, 1724, 2075
Zsp2I ATGCAT 1 cut(s) 2132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.