Rorug06G0505200
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005997
Physical Location & Seq
Reverse (-)
62068429 .. 62068875
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0505200.1

Sequence Viewer

Length: 447 bp
ATGATGCACATGACGTTCTACTGGAGCAAGCAGGTGACGCTCCTGTTCGACTCGTGGAGCACCACCACGTGGCTCGGCTACGCCCTCACGCTCCTCGCCTGCCTCATCGTCCCGGCGTTCTACCAGTACCTCGAGGGCCTGCGCGTGCGCCTCAGGCTTGCGTCTTCGGCGGGGAAGGCGGCGGCGGCGGGGTCCGATCCGATCGAGACTCCGCTCCTGCGAGCCAAGCTCGCCGGAGGAGGGAAGTTCTCTGTGGTTAAGTTGGCCGGAGCCGTGATCTTCGCCGTGAATTCTGCGATCGGGTACATGCTGATGTTGGCGGTGATGTCGTACAACGGCGGCGTTTTCGTGGCGATCGTTTTGGGGTTGGCGATTGGGTATTTGTTTTTCAGGAGTGGAGATGAGGAAGTTTCGGCTGCGGTTGAAAATCCCTGTGCTTGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

148

Amino Acids

15.79

Weight (kDa)

8.47

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctr PF04145 4 - 130 1.3e-22 Ctr copper transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 22
AbsI CCTCGAGG 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 22
AccB7I CCANNNNNTGG 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 214
AccII CGCG 1 cut(s) 144
AciI CCGC 9 cut(s) 170, 179, 182, 185, 188, 212, 320, 339, 419
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 289
AcvI CACGTG 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 3 cut(s) 128, 305, 332
AfiI CCNNNNNNNGG 2 cut(s) 69, 240
AgsI TTSAA 1 cut(s) 425
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 136, 264
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 2 cut(s) 144, 150
AspS9I GGNCC 2 cut(s) 136, 192
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 46, 334
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 192
AxyI CCTNAGG 1 cut(s) 152
BauI CACGAG 1 cut(s) 52
BbrPI CACGTG 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 403
BceAI ACGGC 3 cut(s) 257, 269, 352
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 5 cut(s) 180, 183, 186, 340, 417
BlsI GCNGC 5 cut(s) 181, 184, 187, 341, 418
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 136, 192
BmiI GGNNCC 2 cut(s) 193, 271
BmrFI CCNGG 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 156
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 69
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 240
Bse1I ACTGG 2 cut(s) 26, 124
Bse21I CCTNAGG 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 69, 240
BseMII CTCAG 1 cut(s) 166
BseNI ACTGG 2 cut(s) 26, 124
BseRI GAGGAG 2 cut(s) 83, 252
BseXI GCAGC 1 cut(s) 403
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 3 cut(s) 204, 300, 357
BshFI GGCC 2 cut(s) 138, 266
BsiEI CGRYCG 3 cut(s) 204, 300, 357
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 113, 234, 267
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 69, 240
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 138, 266
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 5 cut(s) 196, 201, 276, 297, 354
BspACI CCGC 9 cut(s) 170, 179, 182, 185, 188, 212, 320, 339, 419
BspANI GGCC 2 cut(s) 138, 266
BspCNI CTCAG 1 cut(s) 165
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 2 cut(s) 193, 271
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 214
BsrI ACTGG 2 cut(s) 26, 124
BssMI GATC 5 cut(s) 196, 201, 276, 297, 354
BssSI CACGAG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 52
BstBAI YACGTR 1 cut(s) 69
BstC8I GCNNGC 7 cut(s) 29, 100, 140, 146, 159, 222, 231
BstDEI CTNAG 2 cut(s) 152, 444
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 2 cut(s) 144, 150
BstKTI GATC 5 cut(s) 199, 204, 279, 300, 357
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 5 cut(s) 196, 201, 276, 297, 354
BstMCI CGRYCG 3 cut(s) 204, 300, 357
BstMWI GCNNNNNNNGC 7 cut(s) 37, 154, 167, 176, 185, 226, 230
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 403
BstV2I GAAGAC 1 cut(s) 156
Bsu36I CCTNAGG 1 cut(s) 152
BsuRI GGCC 2 cut(s) 138, 266
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 7 cut(s) 29, 100, 140, 146, 159, 222, 231
CfoI GCGC 2 cut(s) 144, 150
Cfr13I GGNCC 2 cut(s) 136, 192
CseI GACGC 2 cut(s) 46, 150
Csp6I GTAC 3 cut(s) 127, 304, 331
CviAII CATG 2 cut(s) 10, 307
CviJI RGCY 9 cut(s) 73, 78, 138, 157, 224, 229, 266, 272, 416
CviKI_1 RGCY 9 cut(s) 73, 78, 138, 157, 224, 229, 266, 272, 416
CviQI GTAC 3 cut(s) 127, 304, 331
DdeI CTNAG 2 cut(s) 152, 444
DpnI GATC 5 cut(s) 198, 203, 278, 299, 356
DpnII GATC 5 cut(s) 196, 201, 276, 297, 354
DraIII CACNNNGTG 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 192
Eco72I CACGTG 1 cut(s) 69
Eco81I CCTNAGG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 289
FaeI CATG 2 cut(s) 13, 310
FaiI YATR 2 cut(s) 11, 308
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 9, 306
FauI CCCGC 2 cut(s) 163, 181
Fnu4HI GCNGC 5 cut(s) 180, 183, 186, 340, 417
Fsp4HI GCNGC 5 cut(s) 180, 183, 186, 340, 417
GlaI GCGC 2 cut(s) 143, 149
GluI GCNGC 5 cut(s) 180, 183, 186, 340, 417
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 2 cut(s) 138, 266
HapII CCGG 3 cut(s) 113, 234, 267
HgaI GACGC 2 cut(s) 46, 150
HhaI GCGC 2 cut(s) 144, 150
Hin1II CATG 2 cut(s) 13, 310
Hin6I GCGC 2 cut(s) 142, 148
HinP1I GCGC 2 cut(s) 142, 148
HinfI GANTC 2 cut(s) 50, 208
HpaII CCGG 3 cut(s) 113, 234, 267
HphI GGTGA 2 cut(s) 46, 334
Hpy188I TCNGA 2 cut(s) 196, 201
Hpy188III TCNNGA 2 cut(s) 205, 391
HpyAV CCTTC 1 cut(s) 169
HpyCH4IV ACGT 2 cut(s) 14, 68
HpyCH4V TGCA 1 cut(s) 7
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 154, 167, 176, 185, 226, 230
HpyF3I CTNAG 2 cut(s) 152, 444
HpySE526I ACGT 2 cut(s) 14, 68
Hsp92II CATG 2 cut(s) 13, 310
HspAI GCGC 2 cut(s) 142, 148
Kzo9I GATC 5 cut(s) 196, 201, 276, 297, 354
LmnI GCTCC 6 cut(s) 24, 45, 57, 96, 219, 269
Lsp1109I GCAGC 1 cut(s) 403
MaeII ACGT 2 cut(s) 14, 68
MaeIII GTNAC 1 cut(s) 34
MalI GATC 5 cut(s) 198, 203, 278, 299, 356
MbiI CCGCTC 1 cut(s) 214
MboI GATC 5 cut(s) 196, 201, 276, 297, 354
MboII GAAGA 2 cut(s) 156, 271
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 1 cut(s) 289
MlyI GAGTC 2 cut(s) 44, 202
MnlI CCTC 9 cut(s) 95, 104, 113, 127, 140, 161, 230, 233, 397
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 3 cut(s) 113, 234, 267
MspR9I CCNGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 7 cut(s) 37, 154, 167, 176, 185, 226, 230
NciI CCSGG 1 cut(s) 113
NdeII GATC 5 cut(s) 196, 201, 276, 297, 354
NlaIII CATG 2 cut(s) 13, 310
NlaIV GGNNCC 2 cut(s) 193, 271
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 131
PaqCI CACCTGC 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PflMI CCANNNNNTGG 1 cut(s) 69
PkrI GCNGC 5 cut(s) 181, 184, 187, 341, 418
Ple19I CGATCG 3 cut(s) 204, 300, 357
PleI GAGTC 2 cut(s) 44, 202
PmaCI CACGTG 1 cut(s) 69
PmlI CACGTG 1 cut(s) 69
PpsI GAGTC 2 cut(s) 44, 202
Ppu21I YACGTR 1 cut(s) 69
PspCI CACGTG 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 193, 271
PspPI GGNCC 2 cut(s) 136, 192
PspXI VCTCGAGB 1 cut(s) 131
PvuI CGATCG 3 cut(s) 204, 300, 357
RsaI GTAC 3 cut(s) 128, 305, 332
RsaNI GTAC 3 cut(s) 127, 304, 331
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 5 cut(s) 180, 183, 186, 340, 417
Sau3AI GATC 5 cut(s) 196, 201, 276, 297, 354
Sau96I GGNCC 2 cut(s) 136, 192
SchI GAGTC 2 cut(s) 44, 202
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 62
SetI ASST 5 cut(s) 17, 36, 71, 132, 231
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 1 cut(s) 289
SsiI CCGC 9 cut(s) 170, 179, 182, 185, 188, 212, 320, 339, 419
StyD4I CCNGG 1 cut(s) 111
TaiI ACGT 2 cut(s) 17, 71
TaqI TCGA 3 cut(s) 48, 132, 204
TasI AATT 1 cut(s) 289
TauI GCSGC 4 cut(s) 182, 185, 188, 342
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.