MD14G1159600.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005997
Physical Location & Seq
Reverse (-)
25355851 .. 25359765
3915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1159600.v1.1.491

Sequence Viewer

Length: 2232 bp
ATGGGGTGTTTCAGATCATTTCTGCTGTTCTGTTTTGCATGGAGCTTCTTACTTCTAGGCACTCATCAACTCCAATCCTCACATACCCAAGTGCTTCTGCAGCTCAGAAAGCACTTAGAGTACCCAAAACAACTAGAAATTTGGAACAATCACAGCTTAGATTTCTGCTCGATTTCTTCATTTGGTCAAGTAAACATTACATGCTTGGACAATCTTGTAATTGAGCTCAGAATCAAGGGTGATAAGATTAAGCAATCCAAAGAAAGCGGCTACTTCAACGGCTTTCCAATCCAAAGCCAAACTTTATCAGATTCTTTTTCTTTGGATTCTTTTGTCACTACCCTTTCAAGGCTAAACAGTTTGAGAGATCTTAGCCTTGTGTCTTTGGGCATTTGGGGACAACTTCCAGATAAGATTCATAGGTTGTCTTCCCTTGAACATCTCGATTTGAGCTCGAACTTTCTCTTCGGTTCAATCCCACCTAGGATTTCCGCAATGGCAATGCTTCAAACTTTTAAAATCGATGAGAATTTCTTAAATGATACTATCCCTGATTGGTTTGATTCGCTTTCCAATCTTACAACTCTGAGCTTGAGGAACAACAAGTTGGAAGGTAAATTGCAAGATCTTAGTAGCCTAACCAGTTTACAAGTGCTAGATTTGAGCAGGAACAAATTGAACTCTGTGTTGCCTCCAATGCCGAAAGGGTTGGTCATGCTTTCCGTCAGTGAAAACTCCTTCTCCGGCAAGATTCCAGTGAAATATGGCAAGTTAAGTGGACTTCAACACATTGATATGTCACACAATGCACTTATAGGCACACCCCCTGCTGCAGTCTTCTCTTTGCCTAATATTAGTTACTTGAACTTGGGGTCAAATCTGCTGAGTGGCTCGCTTTCGGGCAATCTAATCTGCGGTAGCAAGCTTAATTTCATCGATATATCGAATAATAGTTTGACGGGTAAGTTGCCTTCATGCTTAAGAAATGAATCAGAAGAGAGAGTTGTGAAGTTTGGTGGGAATTGTTTGTCCATTGGTATGCAAAACCAGCATGAACAAGCACACTGCACGGAAGTGATCAGCCTGAAGAAGAAAGAGTCCGGAGGGAACAATGTAGGGATCTTGGTGGGAGTGATTCTAGGATTAGTTGTACTTAGTGTGCTTCTGGTTTTAGGGTTTATCATTTTCTGCAGAAGATATTGCCTTCGTGGGATATCGGAGCAGCATTTGCTGCATAAATCAGTGCAGGAAAATTCAGCAGTTGGGTTTTCGACCGAGCTTCTAACAAGTGCAAGATTTATTTCACAAGATGGAAAGGTAGGGATACAAGGCCTCCCTGTGTGCCGGACATTTTCTTTGGAAGAGTTGATGGAAGCTACGAAAAATTTCAACAGCTCTGCCTTTTTGGGTGAAGGTTCATATGGGAAGCTTTACAAAGGAAGACTATACAATGGGACTCAAGTTGCTATAAGGTGCCTGCCTTTGTCGAAAAAGTACACAATTCGAAATGTTAAACTTCGGTTGGATCTGCTTTCCAAGCTTCGGCACCAGCATTTGGTGTGTCTTTTAGGGCACTGCCTCGATGGTGGTGGACAAGATGATTACAGTCCGAACAAGGTCTATCTTGTATCTGAATATGTCCCCAATGGGACATTCCGCGCTCATCTCTCTGGGAATAGTCCTGGAAAGGTTCTCAACTGGTCGGAGAGATTGGCAATTCTAGTTAGTGTGGCCAAGGCCGTGCACTTCCTACATACCGGAATTATTCCTGGTTTCTTCTGCAACTGCCTAAAGACGAACAATATCTTGCTCGATGAGCATGGTATGGCAAAATTGAGTGACTATGGACTGTCCATTCTCGCAGAAGAAACCAATAAATCCACGGCAAAAGATGGCCTAACATCATGGCAAATGACAAGTTTAGAGGATGATGCTTATAGCTTCGGATACATACTACTCGAGGCTCTCGTTGGATCTTCCATGCCTTCTCGAAGAGAAGTAATCCTTCAAAATAATATGGCATCACTCAATAGCCAAGACGGGCGGAAGCAGATAGTTGAACCGGCTATTCTAGCCACATGCTCACAAGAGTCTCTGTCGATCGTGATCTCTATAATGAACAAATGCATTTGTCCAGAATCGAGCCGTCCATCTTTCGAGGACATCCTTTGGAACTTACAGTACGCAGTTCAAGTGCAGGAAGAAACAGCAGATGGAGACCAAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

744

Amino Acids

82.11

Weight (kDa)

7.79

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 112 - 225 4e-10 Leucine-rich repeat region
LRR_8 PF13855 169 - 226 4e-09 Leucine rich repeat
LRR_4 PF12799 192 - 228 3.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 237 - 295 3e-06 Leucine rich repeat
Pkinase PF00069 465 - 723 1.3e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 468 - 725 1.5e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1473, 1545
AccB7I CCANNNNNTGG 1 cut(s) 1555
AccII CGCG 1 cut(s) 1659
AccIII TCCGGA 1 cut(s) 1100
AciI CCGC 5 cut(s) 267, 492, 915, 1657, 2044
AclWI GGATC 3 cut(s) 1127, 1533, 1981
AcoI YGGCCR 1 cut(s) 1731
AcsI RAATTY 4 cut(s) 138, 529, 1252, 1384
AcuI CTGAAG 1 cut(s) 1106
AfaI GTAC 4 cut(s) 122, 1152, 1496, 2183
AfiI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
AflII CTTAAG 1 cut(s) 979
AjnI CCWGG 2 cut(s) 1681, 1768
AjuI GAANNNNNNNTTGG 4 cut(s) 590, 622, 1637, 1669
AleI CACNNNNGTG 1 cut(s) 1073
Alw21I GWGCWC 3 cut(s) 228, 455, 1746
Alw26I GTCTC 2 cut(s) 2097, 2211
Alw44I GTGCAC 1 cut(s) 1742
AlwI GGATC 3 cut(s) 1127, 1533, 1981
Ama87I CYCGRG 1 cut(s) 1958
Aor13HI TCCGGA 1 cut(s) 1100
AoxI GGCC 4 cut(s) 1330, 1731, 1737, 1894
ApaLI GTGCAC 1 cut(s) 1742
ApeKI GCWGC 4 cut(s) 100, 830, 1222, 1231
ApoI RAATTY 4 cut(s) 138, 529, 1252, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 1339, 1371
Asp700I GAANNNNTTC 1 cut(s) 1385
AspA2I CCTAGG 1 cut(s) 482
AspLEI GCGC 1 cut(s) 1661
AsuHPI GGTGA 2 cut(s) 251, 1421
AsuII TTCGAA 1 cut(s) 1504
AvaI CYCGRG 1 cut(s) 1958
AvrII CCTAGG 1 cut(s) 482
BaeGI GKGCMC 2 cut(s) 1575, 1746
BalI TGGCCA 1 cut(s) 1733
BanI GGYRCC 2 cut(s) 1473, 1545
BanII GRGCYC 2 cut(s) 228, 455
BarI GAAGNNNNNNTAC 2 cut(s) 955, 987
BbsI GAAGAC 3 cut(s) 420, 829, 1447
Bbv12I GWGCWC 3 cut(s) 228, 455, 1746
BbvI GCAGC 4 cut(s) 112, 817, 1218, 1234
BccI CCATC 6 cut(s) 1304, 1363, 1577, 1886, 2158, 2207
BceAI ACGGC 4 cut(s) 295, 1724, 1899, 2130
BciT130I CCWGG 2 cut(s) 1683, 1770
BciVI GTATCC 2 cut(s) 1317, 1940
BclI TGATCA 1 cut(s) 1077
BcoDI GTCTC 2 cut(s) 2097, 2211
BfaI CTAG 7 cut(s) 56, 134, 483, 656, 1139, 1721, 2072
BfmI CTRYAG 3 cut(s) 98, 831, 1189
BfrI CTTAAG 1 cut(s) 979
BfuI GTATCC 2 cut(s) 1317, 1940
BglII AGATCT 2 cut(s) 367, 625
BisI GCNGC 5 cut(s) 101, 268, 831, 1223, 1232
BlnI CCTAGG 1 cut(s) 482
BlsI GCNGC 5 cut(s) 102, 269, 832, 1224, 1233
Bme1390I CCNGG 2 cut(s) 1683, 1770
BmeT110I CYCGRG 1 cut(s) 1958
BmiI GGNNCC 2 cut(s) 1475, 1547
BmrFI CCNGG 2 cut(s) 1683, 1770
BmsI GCATC 2 cut(s) 1921, 2030
BpiI GAAGAC 3 cut(s) 420, 829, 1447
Bpu14I TTCGAA 1 cut(s) 1504
BpuEI CTTGAG 2 cut(s) 613, 1443
Bsa29I ATCGAT 2 cut(s) 522, 936
BsaBI GATNNNNATC 1 cut(s) 2105
BsaI GGTCTC 1 cut(s) 2211
BsaJI CCNNGG 3 cut(s) 482, 1734, 1881
BsaWI WCCGGW 2 cut(s) 1100, 1757
BsaXI ACNNNNNCTCC 4 cut(s) 725, 755, 1317, 1347
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
Bse118I RCCGGY 1 cut(s) 2062
Bse1I ACTGG 3 cut(s) 642, 755, 1703
Bse3DI GCAATG 2 cut(s) 501, 507
Bse8I GATNNNNATC 1 cut(s) 2105
BseAI TCCGGA 1 cut(s) 1100
BseBI CCWGG 2 cut(s) 1683, 1770
BseCI ATCGAT 2 cut(s) 522, 936
BseDI CCNNGG 3 cut(s) 482, 1734, 1881
BseGI GGATG 2 cut(s) 1933, 2163
BseJI GATNNNNATC 1 cut(s) 2105
BseLI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
BseMI GCAATG 2 cut(s) 501, 507
BseMII CTCAG 4 cut(s) 118, 241, 578, 875
BseNI ACTGG 3 cut(s) 642, 755, 1703
BseSI GKGCMC 2 cut(s) 1575, 1746
BseXI GCAGC 4 cut(s) 112, 817, 1218, 1234
BsgI GTGCAG 3 cut(s) 1051, 1265, 2216
Bsh1236I CGCG 1 cut(s) 1659
Bsh1285I CGRYCG 2 cut(s) 1275, 2103
BshFI GGCC 4 cut(s) 1332, 1733, 1739, 1896
BshNI GGYRCC 2 cut(s) 1473, 1545
BshVI ATCGAT 2 cut(s) 522, 936
BsiEI CGRYCG 2 cut(s) 1275, 2103
BsiHKAI GWGCWC 3 cut(s) 228, 455, 1746
BsiHKCI CYCGRG 1 cut(s) 1958
BsiSI CCGG 5 cut(s) 744, 1101, 1345, 1758, 2063
BslFI GGGAC 4 cut(s) 411, 1468, 1625, 1663
BslI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
BsmAI GTCTC 2 cut(s) 2097, 2211
BsmFI GGGAC 4 cut(s) 411, 1468, 1625, 1663
BsnI GGCC 4 cut(s) 1332, 1733, 1739, 1896
Bso31I GGTCTC 1 cut(s) 2211
BsoBI CYCGRG 1 cut(s) 1958
Bsp119I TTCGAA 1 cut(s) 1504
Bsp1286I GDGCHC 4 cut(s) 228, 455, 1575, 1746
Bsp13I TCCGGA 1 cut(s) 1100
Bsp143I GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
BspACI CCGC 5 cut(s) 267, 492, 915, 1657, 2044
BspANI GGCC 4 cut(s) 1332, 1733, 1739, 1896
BspCNI CTCAG 4 cut(s) 117, 240, 579, 876
BspDI ATCGAT 2 cut(s) 522, 936
BspEI TCCGGA 1 cut(s) 1100
BspFNI CGCG 1 cut(s) 1659
BspLI GGNNCC 2 cut(s) 1475, 1547
BspMAI CTGCAG 3 cut(s) 102, 835, 1193
BspPI GGATC 3 cut(s) 1127, 1533, 1981
BspT104I TTCGAA 1 cut(s) 1504
BspT107I GGYRCC 2 cut(s) 1473, 1545
BspTI CTTAAG 1 cut(s) 979
BspTNI GGTCTC 1 cut(s) 2211
BsrDI GCAATG 2 cut(s) 501, 507
BsrFI RCCGGY 1 cut(s) 2062
BsrI ACTGG 3 cut(s) 642, 755, 1703
BssAI RCCGGY 1 cut(s) 2062
BssECI CCNNGG 3 cut(s) 482, 1734, 1881
BssMI GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
BssT1I CCWWGG 2 cut(s) 482, 1734
Bst2UI CCWGG 2 cut(s) 1683, 1770
Bst4CI ACNGT 4 cut(s) 359, 1607, 1851, 2181
Bst6I CTCTTC 4 cut(s) 470, 990, 1356, 1987
BstAFI CTTAAG 1 cut(s) 979
BstAPI GCANNNNNTGC 2 cut(s) 1228, 1231
BstBI TTCGAA 1 cut(s) 1504
BstC8I GCNNGC 3 cut(s) 893, 923, 1478
BstDEI CTNAG 9 cut(s) 104, 115, 157, 227, 371, 587, 629, 884, 1154
BstDSI CCRYGG 1 cut(s) 1881
BstF5I GGATG 2 cut(s) 1933, 2163
BstFNI CGCG 1 cut(s) 1659
BstHHI GCGC 1 cut(s) 1661
BstKTI GATC 9 cut(s) 17, 370, 628, 1080, 1122, 1528, 1976, 2103, 2109
BstMAI GTCTC 2 cut(s) 2097, 2211
BstMBI GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
BstMCI CGRYCG 2 cut(s) 1275, 2103
BstMWI GCNNNNNNNGC 9 cut(s) 100, 109, 697, 1048, 1228, 1231, 1537, 1816, 2072
BstNI CCWGG 2 cut(s) 1683, 1770
BstNSI RCATGY 2 cut(s) 204, 2082
BstSCI CCNGG 2 cut(s) 1681, 1768
BstSFI CTRYAG 3 cut(s) 98, 831, 1189
BstSLI GKGCMC 2 cut(s) 1575, 1746
BstUI CGCG 1 cut(s) 1659
BstV1I GCAGC 4 cut(s) 112, 817, 1218, 1234
BstV2I GAAGAC 3 cut(s) 420, 829, 1447
BstX2I RGATCY 5 cut(s) 367, 625, 1119, 1525, 1973
BstYI RGATCY 5 cut(s) 367, 625, 1119, 1525, 1973
Bsu15I ATCGAT 2 cut(s) 522, 936
BsuI GTATCC 2 cut(s) 1317, 1940
BsuRI GGCC 4 cut(s) 1332, 1733, 1739, 1896
BsuTUI ATCGAT 2 cut(s) 522, 936
BtgI CCRYGG 1 cut(s) 1881
BtsCI GGATG 2 cut(s) 1933, 2163
BtsI GCAGTG 2 cut(s) 1063, 1573
BtsIMutI CAGTG 5 cut(s) 733, 762, 1063, 1248, 1573
Cac8I GCNNGC 3 cut(s) 893, 923, 1478
CfoI GCGC 1 cut(s) 1661
Cfr10I RCCGGY 1 cut(s) 2062
ClaI ATCGAT 2 cut(s) 522, 936
Csp6I GTAC 4 cut(s) 121, 1151, 1495, 2182
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 9 cut(s) 39, 201, 715, 975, 1052, 1820, 1905, 1981, 2079
CviQI GTAC 4 cut(s) 121, 1151, 1495, 2182
DdeI CTNAG 9 cut(s) 104, 115, 157, 227, 371, 587, 629, 884, 1154
DpnI GATC 9 cut(s) 16, 369, 627, 1079, 1121, 1527, 1975, 2102, 2108
DpnII GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
DraI TTTAAA 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 1731
Eam1104I CTCTTC 4 cut(s) 470, 990, 1356, 1987
EarI CTCTTC 4 cut(s) 470, 990, 1356, 1987
EciI GGCGGA 1 cut(s) 2059
Ecl136II GAGCTC 2 cut(s) 226, 453
Eco130I CCWWGG 2 cut(s) 482, 1734
Eco147I AGGCCT 1 cut(s) 1332
Eco24I GRGCYC 2 cut(s) 228, 455
Eco31I GGTCTC 1 cut(s) 2211
Eco32I GATATC 1 cut(s) 1215
Eco53kI GAGCTC 2 cut(s) 226, 453
Eco57I CTGAAG 1 cut(s) 1106
Eco88I CYCGRG 1 cut(s) 1958
EcoICRI GAGCTC 2 cut(s) 226, 453
EcoRII CCWGG 2 cut(s) 1681, 1768
EcoRV GATATC 1 cut(s) 1215
EcoT14I CCWWGG 2 cut(s) 482, 1734
EcoT22I ATGCAT 1 cut(s) 2129
EcoT38I GRGCYC 2 cut(s) 228, 455
ErhI CCWWGG 2 cut(s) 482, 1734
FaeI CATG 9 cut(s) 42, 204, 718, 978, 1055, 1823, 1908, 1984, 2082
FalI AAGNNNNNCTT 4 cut(s) 286, 318, 1989, 2021
FaqI GGGAC 4 cut(s) 411, 1468, 1625, 1663
FatI CATG 9 cut(s) 38, 200, 714, 974, 1051, 1819, 1904, 1980, 2078
FauNDI CATATG 1 cut(s) 1420
FbaI TGATCA 1 cut(s) 1077
Fnu4HI GCNGC 5 cut(s) 101, 268, 831, 1223, 1232
FokI GGATG 2 cut(s) 1940, 2150
FriOI GRGCYC 2 cut(s) 228, 455
Fsp4HI GCNGC 5 cut(s) 101, 268, 831, 1223, 1232
FspBI CTAG 7 cut(s) 56, 134, 483, 656, 1139, 1721, 2072
GlaI GCGC 1 cut(s) 1660
GluI GCNGC 5 cut(s) 101, 268, 831, 1223, 1232
HaeIII GGCC 4 cut(s) 1332, 1733, 1739, 1896
HapII CCGG 5 cut(s) 744, 1101, 1345, 1758, 2063
HhaI GCGC 1 cut(s) 1661
Hin1II CATG 9 cut(s) 42, 204, 718, 978, 1055, 1823, 1908, 1984, 2082
Hin6I GCGC 1 cut(s) 1659
HinP1I GCGC 1 cut(s) 1659
HindIII AAGCTT 3 cut(s) 923, 1427, 1538
HpaII CCGG 5 cut(s) 744, 1101, 1345, 1758, 2063
HphI GGTGA 2 cut(s) 251, 1421
Hpy166II GTNNAC 6 cut(s) 193, 647, 779, 1497, 1592, 1744
Hpy188III TCNNGA 6 cut(s) 407, 443, 1101, 1989, 2104, 2135
Hpy8I GTNNAC 6 cut(s) 193, 647, 779, 1497, 1592, 1744
HpyAV CCTTC 7 cut(s) 605, 748, 981, 1214, 1406, 1995, 2015
HpyCH4III ACNGT 4 cut(s) 359, 1607, 1851, 2181
HpyF10VI GCNNNNNNNGC 9 cut(s) 100, 109, 697, 1048, 1228, 1231, 1537, 1816, 2072
HpyF3I CTNAG 9 cut(s) 104, 115, 157, 227, 371, 587, 629, 884, 1154
Hsp92II CATG 9 cut(s) 42, 204, 718, 978, 1055, 1823, 1908, 1984, 2082
HspAI GCGC 1 cut(s) 1659
Kpn2I TCCGGA 1 cut(s) 1100
Ksp22I TGATCA 1 cut(s) 1077
Kzo9I GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
LmnI GCTCC 2 cut(s) 42, 1219
Lsp1109I GCAGC 4 cut(s) 112, 817, 1218, 1234
LweI GCATC 2 cut(s) 1921, 2030
MaeI CTAG 7 cut(s) 56, 134, 483, 656, 1139, 1721, 2072
MaeIII GTNAC 4 cut(s) 334, 798, 857, 1838
MalI GATC 9 cut(s) 16, 369, 627, 1079, 1121, 1527, 1975, 2102, 2108
MboI GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
MflI RGATCY 5 cut(s) 367, 625, 1119, 1525, 1973
MhlI GDGCHC 4 cut(s) 228, 455, 1575, 1746
MlsI TGGCCA 1 cut(s) 1733
MluNI TGGCCA 1 cut(s) 1733
MlyI GAGTC 3 cut(s) 1106, 1450, 2099
MmeI TCCRAC 4 cut(s) 588, 1503, 1683, 1951
MnlI CCTC 9 cut(s) 88, 588, 702, 1097, 1343, 1589, 1918, 1954, 2152
Mox20I TGGCCA 1 cut(s) 1733
Mph1103I ATGCAT 1 cut(s) 2129
MroI TCCGGA 1 cut(s) 1100
MroXI GAANNNNTTC 1 cut(s) 1385
MscI TGGCCA 1 cut(s) 1733
MseI TTAA 7 cut(s) 249, 516, 536, 773, 927, 980, 1512
MslI CAYNNNNRTG 3 cut(s) 794, 1037, 1073
Msp20I TGGCCA 1 cut(s) 1733
MspCI CTTAAG 1 cut(s) 979
MspI CCGG 5 cut(s) 744, 1101, 1345, 1758, 2063
MspR9I CCNGG 2 cut(s) 1683, 1770
MvaI CCWGG 2 cut(s) 1683, 1770
MvnI CGCG 1 cut(s) 1659
MwoI GCNNNNNNNGC 9 cut(s) 100, 109, 697, 1048, 1228, 1231, 1537, 1816, 2072
NdeI CATATG 1 cut(s) 1420
NdeII GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
NlaIII CATG 9 cut(s) 42, 204, 718, 978, 1055, 1823, 1908, 1984, 2082
NlaIV GGNNCC 2 cut(s) 1475, 1547
NmuCI GTSAC 3 cut(s) 334, 798, 1838
NsiI ATGCAT 1 cut(s) 2129
NspI RCATGY 2 cut(s) 204, 2082
NspV TTCGAA 1 cut(s) 1504
OliI CACNNNNGTG 1 cut(s) 1073
PaeR7I CTCGAG 1 cut(s) 1958
PceI AGGCCT 1 cut(s) 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 1965
PdmI GAANNNNTTC 1 cut(s) 1385
PfeI GAWTC 9 cut(s) 231, 311, 326, 415, 563, 751, 989, 1135, 2138
PflMI CCANNNNNTGG 1 cut(s) 1555
PfoI TCCNGGA 1 cut(s) 1681
PkrI GCNGC 5 cut(s) 102, 269, 832, 1224, 1233
Ple19I CGATCG 1 cut(s) 2103
PleI GAGTC 3 cut(s) 1105, 1450, 2098
PpsI GAGTC 3 cut(s) 1105, 1450, 2098
Psp124BI GAGCTC 2 cut(s) 228, 455
Psp6I CCWGG 2 cut(s) 1681, 1768
PspGI CCWGG 2 cut(s) 1681, 1768
PspN4I GGNNCC 2 cut(s) 1475, 1547
PspXI VCTCGAGB 1 cut(s) 1958
PsrI GAACNNNNNNTAC 2 cut(s) 2165, 2197
PstI CTGCAG 3 cut(s) 102, 835, 1193
PsuI RGATCY 5 cut(s) 367, 625, 1119, 1525, 1973
PvuI CGATCG 1 cut(s) 2103
RsaI GTAC 4 cut(s) 122, 1152, 1496, 2183
RsaNI GTAC 4 cut(s) 121, 1151, 1495, 2182
RseI CAYNNNNRTG 3 cut(s) 794, 1037, 1073
SacI GAGCTC 2 cut(s) 228, 455
SaqAI TTAA 7 cut(s) 249, 516, 536, 773, 927, 980, 1512
SatI GCNGC 5 cut(s) 101, 268, 831, 1223, 1232
Sau3AI GATC 9 cut(s) 14, 367, 625, 1077, 1119, 1525, 1973, 2100, 2106
SchI GAGTC 3 cut(s) 1106, 1450, 2099
ScrFI CCNGG 2 cut(s) 1683, 1770
SduI GDGCHC 4 cut(s) 228, 455, 1575, 1746
SfaNI GCATC 2 cut(s) 1921, 2030
SfcI CTRYAG 3 cut(s) 98, 831, 1189
Sfr274I CTCGAG 1 cut(s) 1958
SfuI TTCGAA 1 cut(s) 1504
SlaI CTCGAG 1 cut(s) 1958
SmiMI CAYNNNNRTG 3 cut(s) 794, 1037, 1073
SmlI CTYRAG 4 cut(s) 592, 979, 1458, 1958
SmoI CTYRAG 4 cut(s) 592, 979, 1458, 1958
SseBI AGGCCT 1 cut(s) 1332
SsiI CCGC 5 cut(s) 267, 492, 915, 1657, 2044
SspI AATATT 1 cut(s) 853
SspMI CTAG 7 cut(s) 56, 134, 483, 656, 1139, 1721, 2072
SstI GAGCTC 2 cut(s) 228, 455
StuI AGGCCT 1 cut(s) 1332
StyD4I CCNGG 2 cut(s) 1681, 1768
StyI CCWWGG 2 cut(s) 482, 1734
TaaI ACNGT 4 cut(s) 359, 1607, 1851, 2181
TaqII GACCGA 1 cut(s) 1289
TatI WGTACW 2 cut(s) 1150, 1494
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 9 cut(s) 231, 311, 326, 415, 563, 751, 989, 1135, 2138
Tru1I TTAA 7 cut(s) 249, 516, 536, 773, 927, 980, 1512
Tru9I TTAA 7 cut(s) 249, 516, 536, 773, 927, 980, 1512
TscAI CASTG 5 cut(s) 733, 762, 1070, 1248, 1580
TseFI GTSAC 3 cut(s) 334, 798, 1838
TseI GCWGC 4 cut(s) 100, 830, 1222, 1231
Tsp45I GTSAC 3 cut(s) 334, 798, 1838
TspDTI ATGAA 8 cut(s) 168, 407, 922, 963, 1002, 1068, 1407, 2132
TspGWI ACGGA 2 cut(s) 712, 1085
TspRI CASTG 5 cut(s) 733, 762, 1070, 1248, 1580
Van91I CCANNNNNTGG 1 cut(s) 1555
Vha464I CTTAAG 1 cut(s) 979
VneI GTGCAC 1 cut(s) 1742
XapI RAATTY 4 cut(s) 138, 529, 1252, 1384
XceI RCATGY 2 cut(s) 204, 2082
XhoI CTCGAG 1 cut(s) 1958
XmaJI CCTAGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 1385
XspI CTAG 7 cut(s) 56, 134, 483, 656, 1139, 1721, 2072
Zsp2I ATGCAT 1 cut(s) 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.