FvH4_5g08580

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006574
Physical Location & Seq
Reverse (-)
4940285 .. 4944052
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08580.t3

Sequence Viewer

Length: 1236 bp
ATGGACTGGAACATGAAAGCATCATCTTCGTGGGATTTGAGTGAACTTGAGCAAGCAACCTTTTCCAACTTGGACACAGTGAATGAGTCTAGTAGCTTTGGAGATCAACATAGGGCCATCAAAGGAGACTTTTCTGTTGATTTGAAGCTTGGTCAGTTGGGTGATTCTGGTAATAATCAGTTGCTGGACATGTTCAAGCAACATGGAGGTCCTAAACCTTCATCGTCATCACCTTCCGGGGCTTCAAAGAGGTCAAGGGCAGCTTATAATGGATCCCACACAGTTTCTTGCCTCGTTGACGGGTGTAGATCAGACCTTAGTACTTGTAGGGATTACCATAGGCGCCATAAGGTCTGTGAACTACATTCTAAGACTCCAATGGTCACAATAAATGGCCAGAAGCAACGATTTTGCCAACAATGCAGCAGGTTCCATTCATTGGAGGAATTTGACGAAGGGAAGAGAAGCTGCAGGAAACGTCTTGATGGACACAACCGTAGGAGAAGGAAGCCTCAACCCGAACCCTTGTCGCGTTCAGGAAGCTTCTTATCCAACTACCAAGGTACTCAATTGTCGCCATTCTCTAGCTCACTTGTATATCCCTCCACCACTGTTGTGAACCCAACATGGGCTGCAGTTGTCAAAGCTGAAGCCGATACCGGGCATCATAACCAACATCTACAACATCATAACATGTTTCTCGGGTCCTCAACCAGAAGCAGCAGCAGCTACAATAAGGGAGGGGCAAACAAGCAGCAATTCACACTTTTCCAAAGTAGCAACAACAACCAAACTCATGGAGCTTCTGTATGCCAGCCCCTTCTGAATACCATTTCAATGTCAGATACTGCTTCTTCAGGGGCTGCTGCGTCTGCAAAGAGCAAACTGTGTCATGACAGGTTAACAACAACTCGAATCCTCCACGATTCGGATTGTGCTCTCTCTCTTCTGTCATCACCGCAGACGCAGCAGCAGACATCTGAGTTGGGTTTGAGACACATGATGCAGCAGCCTAATACGACATCTCTCATGCACCACCACCGCCTCGGCTTTCATAGGAACAACAACAGCAACAATATAGAGCCAATGGACTCAGTTCGGGTCTCCCATGGATCAAGTGACGATGCACATAATGTTCATCAGTCCTCAGGAATATTCCACATGGGCTCTGATGGGTCACAGCAGGGGAATCATCATCATCAAGGCTCTCAAACTCTTCCTTTTCATTGGCAGTAG

Protein Analysis

412

Amino Acids

45.5

Weight (kDa)

9.0

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 83 - 110 2.8e-08 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 439
AccII CGCG 1 cut(s) 532
AciI CCGC 2 cut(s) 959, 1042
AclWI GGATC 3 cut(s) 267, 280, 1120
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 2 cut(s) 669, 840
AcyI GRCGYC 1 cut(s) 343
AfaI GTAC 2 cut(s) 322, 565
AfiI CCNNNNNNNGG 4 cut(s) 439, 628, 660, 928
AflIII ACRYGT 2 cut(s) 189, 693
AgsI TTSAA 4 cut(s) 145, 196, 246, 837
AleI CACNNNNGTG 1 cut(s) 614
AluBI AGCT 9 cut(s) 96, 148, 263, 468, 543, 588, 647, 729, 803
AluI AGCT 9 cut(s) 96, 148, 263, 468, 543, 588, 647, 729, 803
Alw21I GWGCWC 1 cut(s) 940
Alw26I GTCTC 3 cut(s) 120, 988, 1108
AlwI GGATC 3 cut(s) 267, 280, 1120
AlwNI CAGNNNCTG 3 cut(s) 184, 848, 863
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 2 cut(s) 114, 394
ApoI RAATTY 1 cut(s) 446
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 3 cut(s) 114, 209, 705
AsuC2I CCSGG 2 cut(s) 238, 661
AsuHPI GGTGA 3 cut(s) 173, 222, 948
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 2 cut(s) 209, 705
AxyI CCTNAGG 1 cut(s) 1147
BalI TGGCCA 1 cut(s) 396
BamHI GGATCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 1169
Bbv12I GWGCWC 1 cut(s) 940
BccI CCATC 3 cut(s) 125, 479, 1166
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BcnI CCSGG 2 cut(s) 238, 661
BcoDI GTCTC 3 cut(s) 120, 988, 1108
BfaI CTAG 2 cut(s) 90, 585
BfmI CTRYAG 2 cut(s) 469, 633
BfoI RGCGCY 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 417
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 238, 661
Bme18I GGWCC 2 cut(s) 209, 705
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 114, 209, 705
BmiI GGNNCC 4 cut(s) 274, 344, 431, 706
BmrFI CCNGG 2 cut(s) 238, 661
BmsI GCATC 4 cut(s) 29, 673, 993, 1114
BpuEI CTTGAG 1 cut(s) 68
BpuMI CCSGG 2 cut(s) 238, 661
BsaHI GRCGYC 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 1108
BsaJI CCNNGG 4 cut(s) 237, 559, 1045, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 792, 822
Bsc4I CCNNNNNNNGG 4 cut(s) 439, 628, 660, 928
Bse1I ACTGG 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 1147
BseDI CCNNGG 4 cut(s) 237, 559, 1045, 1108
BseLI CCNNNNNNNGG 4 cut(s) 439, 628, 660, 928
BseMII CTCAG 3 cut(s) 972, 1107, 1161
BseNI ACTGG 1 cut(s) 11
Bsh1236I CGCG 1 cut(s) 532
BshFI GGCC 2 cut(s) 116, 396
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 940
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 2 cut(s) 237, 660
BslI CCNNNNNNNGG 4 cut(s) 439, 628, 660, 928
BsmAI GTCTC 3 cut(s) 120, 988, 1108
BsnI GGCC 2 cut(s) 116, 396
Bso31I GGTCTC 1 cut(s) 1108
BsoBI CYCGRG 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 940, 1169
Bsp143I GATC 4 cut(s) 103, 272, 308, 1112
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 2 cut(s) 959, 1042
BspANI GGCC 2 cut(s) 116, 396
BspCNI CTCAG 3 cut(s) 973, 1106, 1160
BspFNI CGCG 1 cut(s) 532
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 4 cut(s) 274, 344, 431, 706
BspMAI CTGCAG 2 cut(s) 473, 637
BspMI ACCTGC 1 cut(s) 417
BspPI GGATC 3 cut(s) 267, 280, 1120
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 1108
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 4 cut(s) 237, 559, 1045, 1108
BssMI GATC 4 cut(s) 103, 272, 308, 1112
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 559, 1108
Bst4CI ACNGT 5 cut(s) 79, 283, 497, 613, 888
Bst6I CTCTTC 3 cut(s) 455, 951, 1221
BstACI GRCGYC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 54, 815
BstDEI CTNAG 5 cut(s) 317, 369, 981, 1093, 1147
BstDSI CCRYGG 1 cut(s) 1108
BstFNI CGCG 1 cut(s) 532
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 4 cut(s) 106, 275, 311, 1115
BstMAI GTCTC 3 cut(s) 120, 988, 1108
BstMBI GATC 4 cut(s) 103, 272, 308, 1112
BstMWI GCNNNNNNNGC 4 cut(s) 420, 726, 872, 967
BstNSI RCATGY 2 cut(s) 193, 697
BstSCI CCNGG 2 cut(s) 236, 659
BstSFI CTRYAG 2 cut(s) 469, 633
BstUI CGCG 1 cut(s) 532
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 1147
BsuRI GGCC 2 cut(s) 116, 396
BtgI CCRYGG 1 cut(s) 1108
BtsIMutI CAGTG 2 cut(s) 84, 609
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 54, 815
CaiI CAGNNNCTG 3 cut(s) 184, 848, 863
CciI TCATGA 1 cut(s) 892
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 3 cut(s) 114, 209, 705
CseI GACGC 2 cut(s) 858, 973
Csp6I GTAC 2 cut(s) 321, 564
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviQI GTAC 2 cut(s) 321, 564
DdeI CTNAG 5 cut(s) 317, 369, 981, 1093, 1147
DinI GGCGCC 1 cut(s) 344
DpnI GATC 4 cut(s) 105, 274, 310, 1114
DpnII GATC 4 cut(s) 103, 272, 308, 1112
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 3 cut(s) 455, 951, 1221
EarI CTCTTC 3 cut(s) 455, 951, 1221
Eco130I CCWWGG 2 cut(s) 559, 1108
Eco24I GRGCYC 1 cut(s) 1169
Eco31I GGTCTC 1 cut(s) 1108
Eco47I GGWCC 2 cut(s) 209, 705
Eco57I CTGAAG 2 cut(s) 669, 840
Eco81I CCTNAGG 1 cut(s) 1147
Eco88I CYCGRG 1 cut(s) 701
EcoO109I RGGNCCY 2 cut(s) 209, 705
EcoT14I CCWWGG 2 cut(s) 559, 1108
EcoT38I GRGCYC 1 cut(s) 1169
EgeI GGCGCC 1 cut(s) 344
EheI GGCGCC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 559, 1108
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FriOI GRGCYC 1 cut(s) 1169
FspBI CTAG 2 cut(s) 90, 585
GlaI GCGC 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 2 cut(s) 116, 396
HapII CCGG 2 cut(s) 237, 660
HgaI GACGC 2 cut(s) 858, 973
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 343
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HincII GTYRAC 2 cut(s) 298, 903
HindII GTYRAC 2 cut(s) 298, 903
HindIII AAGCTT 2 cut(s) 146, 541
HinfI GANTC 7 cut(s) 86, 164, 373, 915, 926, 1091, 1189
HpaI GTTAAC 1 cut(s) 903
HpaII CCGG 2 cut(s) 237, 660
HphI GGTGA 3 cut(s) 173, 222, 948
Hpy166II GTNNAC 5 cut(s) 44, 298, 359, 619, 903
Hpy188I TCNGA 6 cut(s) 313, 825, 844, 931, 982, 1171
Hpy188III TCNNGA 4 cut(s) 482, 537, 893, 1149
Hpy8I GTNNAC 5 cut(s) 44, 298, 359, 619, 903
HpyAV CCTTC 5 cut(s) 228, 243, 449, 498, 830
HpyCH4III ACNGT 5 cut(s) 79, 283, 497, 613, 888
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 7 cut(s) 423, 471, 635, 875, 1006, 1033, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 420, 726, 872, 967
HpyF3I CTNAG 5 cut(s) 317, 369, 981, 1093, 1147
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 1 cut(s) 343
HspAI GCGC 1 cut(s) 343
KasI GGCGCC 1 cut(s) 342
KspAI GTTAAC 1 cut(s) 903
Kzo9I GATC 4 cut(s) 103, 272, 308, 1112
LmnI GCTCC 1 cut(s) 800
LweI GCATC 4 cut(s) 29, 673, 993, 1114
MaeI CTAG 2 cut(s) 90, 585
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 3 cut(s) 382, 1118, 1176
MalI GATC 4 cut(s) 105, 274, 310, 1114
MboI GATC 4 cut(s) 103, 272, 308, 1112
MboII GAAGA 5 cut(s) 18, 472, 846, 938, 1208
MfeI CAATTG 1 cut(s) 569
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 2 cut(s) 940, 1169
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 3 cut(s) 446, 569, 758
MluNI TGGCCA 1 cut(s) 396
Mly113I GGCGCC 1 cut(s) 343
MlyI GAGTC 3 cut(s) 95, 367, 1085
MmeI TCCRAC 2 cut(s) 90, 576
Mox20I TGGCCA 1 cut(s) 396
MscI TGGCCA 1 cut(s) 396
MseI TTAA 1 cut(s) 902
MslI CAYNNNNRTG 3 cut(s) 28, 614, 836
Msp20I TGGCCA 1 cut(s) 396
MspI CCGG 2 cut(s) 237, 660
MspR9I CCNGG 2 cut(s) 238, 661
MunI CAATTG 1 cut(s) 569
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 4 cut(s) 420, 726, 872, 967
NarI GGCGCC 1 cut(s) 343
NciI CCSGG 2 cut(s) 238, 661
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 4 cut(s) 103, 272, 308, 1112
NlaIV GGNNCC 4 cut(s) 274, 344, 431, 706
NmeAIII GCCGAG 1 cut(s) 1026
NmuCI GTSAC 3 cut(s) 382, 1118, 1176
NspI RCATGY 2 cut(s) 193, 697
OliI CACNNNNGTG 1 cut(s) 614
PagI TCATGA 1 cut(s) 892
PciI ACATGT 2 cut(s) 189, 693
PfeI GAWTC 4 cut(s) 164, 915, 926, 1189
PflMI CCANNNNNTGG 1 cut(s) 439
PleI GAGTC 3 cut(s) 94, 367, 1085
PluTI GGCGCC 1 cut(s) 346
PpsI GAGTC 3 cut(s) 94, 367, 1085
PpuMI RGGWCCY 2 cut(s) 209, 705
PscI ACATGT 2 cut(s) 189, 693
PsiI TTATAA 1 cut(s) 267
Psp5II RGGWCCY 2 cut(s) 209, 705
PspN4I GGNNCC 4 cut(s) 274, 344, 431, 706
PspPI GGNCC 3 cut(s) 114, 209, 705
PspPPI RGGWCCY 2 cut(s) 209, 705
PstI CTGCAG 2 cut(s) 473, 637
PstNI CAGNNNCTG 3 cut(s) 184, 848, 863
PsuI RGATCY 1 cut(s) 272
RsaI GTAC 2 cut(s) 322, 565
RsaNI GTAC 2 cut(s) 321, 564
RseI CAYNNNNRTG 3 cut(s) 28, 614, 836
SaqAI TTAA 1 cut(s) 902
Sau3AI GATC 4 cut(s) 103, 272, 308, 1112
Sau96I GGNCC 3 cut(s) 114, 209, 705
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 3 cut(s) 95, 367, 1085
ScrFI CCNGG 2 cut(s) 238, 661
SduI GDGCHC 2 cut(s) 940, 1169
SfaNI GCATC 4 cut(s) 29, 673, 993, 1114
SfcI CTRYAG 2 cut(s) 469, 633
SfoI GGCGCC 1 cut(s) 344
SinI GGWCC 2 cut(s) 209, 705
SmiMI CAYNNNNRTG 3 cut(s) 28, 614, 836
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 3 cut(s) 446, 569, 758
SsiI CCGC 2 cut(s) 959, 1042
SspDI GGCGCC 1 cut(s) 342
SspI AATATT 1 cut(s) 1155
SspMI CTAG 2 cut(s) 90, 585
StyD4I CCNGG 2 cut(s) 236, 659
StyI CCWWGG 2 cut(s) 559, 1108
TaaI ACNGT 5 cut(s) 79, 283, 497, 613, 888
TaiI ACGT 1 cut(s) 481
TaqI TCGA 1 cut(s) 913
TasI AATT 3 cut(s) 446, 569, 758
TatI WGTACW 1 cut(s) 320
TfiI GAWTC 4 cut(s) 164, 915, 926, 1189
Tru1I TTAA 1 cut(s) 902
Tru9I TTAA 1 cut(s) 902
TscAI CASTG 2 cut(s) 84, 616
TseFI GTSAC 3 cut(s) 382, 1118, 1176
Tsp45I GTSAC 3 cut(s) 382, 1118, 1176
TspDTI ATGAA 6 cut(s) 29, 210, 426, 1043, 1127, 1214
TspRI CASTG 2 cut(s) 84, 616
Van91I CCANNNNNTGG 1 cut(s) 439
VpaK11BI GGWCC 2 cut(s) 209, 705
XapI RAATTY 1 cut(s) 446
XceI RCATGY 2 cut(s) 193, 697
XspI CTAG 2 cut(s) 90, 585
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.