Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0014479

Physical Location & Seq

Genomic Coordinates Forward (+)

6071794 .. 6073805

Gene Structure 2012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g10690.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGTCTGCAGAGGATCAAATGAATCTCTCTATAAATGGTCAGTCTCAGGTCCCTCCAGGCTTTCGCTTCCATCCTACGGAAGAAGAGCTTCTTCACTACTATCTGAGGAAGAAAGTTGCCTATGAGAGGATTGATCTTGACGTCATTCGAGAGGTTGATCTTAACAAGCTCGAGCCATGGGACATTCAAGAGAAGTGCAAGATAGGTTCCACTCCGCAGAACGATTGGTACTTCTTCAGCCACAAGGACAAGAAATACCCAACTGGTACTCGAACAAATCGTGCAACTGCTGCCGGATTCTGGAAGGCCACCGGCCGTGACAAGATCATCTACAGCGGCATTCGAAGAATCGGATTGCGAAAGACGCTTGTGTTTTACAAGGGACGAGCGCCTCACGGTCAAAAGTCGGATTGGATCATGCATGAATACAGACTTGATGACAGCAGCAGCAGTCTTGATACCACAGTTTCTAATTCAATTGGGGAGTCGATGACCGAAGACGGGTGGGTGGTTTGCAGGGTCTTCAAGAAGAAGAACTACCAGAAAGCAATGGAGAGCCCTAAAGCCTCCTTCTCAATGGACTCGCAAAACAACCAAATGCTTCCGGGTTCAAGAAACGACGGCGTTCTCGATCAGATTCTATTGTACATGGGAAGAAACACTTGCAAGATCGAAAACCATGACTCACTTCATCATGAAAGGTTCATGCATCTTCCACGGCTCGAGAGCCAAACTTCTCTTCCCTCACTTCCAGTCCAGTTCGATCACAACCGTAGCTTCAAAGCTTGTTATCATGATCAGACCATTGATGACATGCTTATAGAAACCGATCAGCAGCAACAACAACAACAACCTTCTCCAACAAACCAATCCAACGACCTGGTTCATGATCATCATGATCATGATGACCCCAAAACAAGAATAGTCAATGACTGGGCGACCCTCGATCGGCTCGTGGCATCTCAACTCAATGGCCATGAAGACGAGACCTCAAAGCACTTGTCCTGCTTCGGTGACCCTAACATGGCCTTTTACTCTTCTTCTCCTTCTAACGACGGGGACTTACAGTTGTCCTATCCATACCTACGTCAAGGAAGAATATCACATAATCAACCCGAAGTCTTCAACAGTGAGAACGATCTCTGGAGCTTCACAAAATCATCGCCGTCGCCGTCATCATCAGACCCATTATGCCACCTGTCGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009832 GO:0009834 GO:0009889 GO:0009891 GO:0009893 GO:0009900 GO:0009987 GO:0010047 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032504 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044085 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048609 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071554 GO:0071669 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

403

Amino Acids

46.32

Weight (kDa)

6.01

Isoelectric Point (pI)

58.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	18 - 145	7.5e-45	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014479)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32770
fragaria_vesca	FvH4_5g10690
malus_domestica	MD06G1121400.v1.1 MD14G1137900.v1.1
prunus_persica	Prupe.5G131900_v2.0.a1
pyrus_communis	pycom06g11260 pycom14g11310
rosa_chinensis	RchiOBHm_Chr7g0187451
rosa_laevigata	RLG00000004780
rosa_multiflora	Rmu_sc0001230.1_g000011
rosa_roxburghii	Rroxscaffold_3G00267410
rosa_rugosa	Rorug06G0481700
rosa_samantha	Rh7AG086200 Rh7BG087700 Rh7CG087100 Rh7DG088700
rosa_wichuraiana	Rw7G007610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	144
AciI	CCGC	2 cut(s)	215, 336
AclWI	GGATC	2 cut(s)	21, 422
AcoI	YGGCCR	2 cut(s)	313, 973
AcuI	CTGAAG	1 cut(s)	220
AcyI	GRCGYC	1 cut(s)	141
AfaI	GTAC	3 cut(s)	230, 268, 647
AfiI	CCNNNNNNNGG	7 cut(s)	76, 126, 300, 501, 948, 1024, 1201
AgsI	TTSAA	6 cut(s)	188, 477, 526, 612, 781, 1126
AjnI	CCWGG	2 cut(s)	55, 879
AjuI	GAANNNNNNNTTGG	2 cut(s)	723, 755
AluBI	AGCT	5 cut(s)	88, 169, 777, 785, 1149
AluI	AGCT	5 cut(s)	88, 169, 777, 785, 1149
Alw26I	GTCTC	2 cut(s)	48, 980
AlwI	GGATC	2 cut(s)	21, 422
Ama87I	CYCGRG	2 cut(s)	170, 722
AoxI	GGCC	4 cut(s)	306, 313, 973, 1026
ApeKI	GCWGC	4 cut(s)	290, 444, 447, 835
ArsI	GACNNNNNNTTYG	2 cut(s)	986, 1018
Asp700I	GAANNNNTTC	1 cut(s)	87
AspLEI	GCGC	1 cut(s)	391
AspS9I	GGNCC	1 cut(s)	49
AsuC2I	CCSGG	1 cut(s)	606
AsuHPI	GGTGA	1 cut(s)	1025
AsuII	TTCGAA	1 cut(s)	343
AvaI	CYCGRG	2 cut(s)	170, 722
AvaII	GGWCC	1 cut(s)	49
BaeI	ACNNNNGTAYC	2 cut(s)	450, 483
BalI	TGGCCA	1 cut(s)	975
BanII	GRGCYC	1 cut(s)	560
BarI	GAAGNNNNNNTAC	2 cut(s)	521, 553
BauI	CACGAG	1 cut(s)	953
BbsI	GAAGAC	4 cut(s)	504, 514, 987, 1114
BbvI	GCAGC	4 cut(s)	277, 456, 459, 847
BccI	CCATC	1 cut(s)	78
BceAI	ACGGC	5 cut(s)	300, 637, 734, 1150, 1156
BciT130I	CCWGG	2 cut(s)	57, 881
BclI	TGATCA	3 cut(s)	796, 889, 898
BcnI	CCSGG	1 cut(s)	606
BcoDI	GTCTC	2 cut(s)	48, 980
BfmI	CTRYAG	2 cut(s)	6, 331
BfoI	RGCGCY	1 cut(s)	392
BisI	GCNGC	5 cut(s)	291, 337, 445, 448, 836
BlsI	GCNGC	5 cut(s)	292, 338, 446, 449, 837
Bme1390I	CCNGG	3 cut(s)	57, 606, 881
Bme18I	GGWCC	1 cut(s)	49
BmeT110I	CYCGRG	2 cut(s)	170, 722
BmgT120I	GGNCC	1 cut(s)	49
BmiI	GGNNCC	2 cut(s)	51, 208
BmrFI	CCNGG	3 cut(s)	57, 606, 881
BmrI	ACTGGG	1 cut(s)	943
BmsI	GCATC	2 cut(s)	718, 968
BmuI	ACTGGG	1 cut(s)	943
BpiI	GAAGAC	4 cut(s)	504, 514, 987, 1114
BpmI	CTGGAG	2 cut(s)	39, 1165
Bpu14I	TTCGAA	1 cut(s)	343
BpuMI	CCSGG	1 cut(s)	606
BsaHI	GRCGYC	1 cut(s)	141
BsaI	GGTCTC	1 cut(s)	980
BsaJI	CCNNGG	2 cut(s)	176, 716
Bsc4I	CCNNNNNNNGG	7 cut(s)	76, 126, 300, 501, 948, 1024, 1201
Bse118I	RCCGGY	1 cut(s)	311
Bse1I	ACTGG	4 cut(s)	268, 752, 757, 938
Bse3DI	GCAATG	1 cut(s)	555
BseBI	CCWGG	2 cut(s)	57, 881
BseDI	CCNNGG	2 cut(s)	176, 716
BseGI	GGATG	1 cut(s)	70
BseLI	CCNNNNNNNGG	7 cut(s)	76, 126, 300, 501, 948, 1024, 1201
BseMI	GCAATG	1 cut(s)	555
BseMII	CTCAG	2 cut(s)	59, 95
BseNI	ACTGG	4 cut(s)	268, 752, 757, 938
BseX3I	CGGCCG	1 cut(s)	313
BseXI	GCAGC	4 cut(s)	277, 456, 459, 847
Bsh1285I	CGRYCG	2 cut(s)	316, 949
BshFI	GGCC	4 cut(s)	308, 315, 975, 1028
BsiEI	CGRYCG	2 cut(s)	316, 949
BsiHKCI	CYCGRG	2 cut(s)	170, 722
BsiSI	CCGG	3 cut(s)	294, 312, 605
BslFI	GGGAC	4 cut(s)	35, 194, 396, 1073
BslI	CCNNNNNNNGG	7 cut(s)	76, 126, 300, 501, 948, 1024, 1201
BsmAI	GTCTC	2 cut(s)	48, 980
BsmFI	GGGAC	4 cut(s)	35, 194, 396, 1073
BsmI	GAATGC	1 cut(s)	339
BsnI	GGCC	4 cut(s)	308, 315, 975, 1028
Bso31I	GGTCTC	1 cut(s)	980
BsoBI	CYCGRG	2 cut(s)	170, 722
Bsp119I	TTCGAA	1 cut(s)	343
Bsp1286I	GDGCHC	1 cut(s)	560
Bsp1407I	TGTACA	1 cut(s)	645
Bsp19I	CCATGG	1 cut(s)	176
BspACI	CCGC	2 cut(s)	215, 336
BspANI	GGCC	4 cut(s)	308, 315, 975, 1028
BspCNI	CTCAG	2 cut(s)	58, 96
BspHI	TCATGA	5 cut(s)	694, 793, 886, 895, 901
BspLI	GGNNCC	2 cut(s)	51, 208
BspMAI	CTGCAG	1 cut(s)	10
BspPI	GGATC	2 cut(s)	21, 422
BspQI	GCTCTTC	1 cut(s)	78
BspT104I	TTCGAA	1 cut(s)	343
BspTNI	GGTCTC	1 cut(s)	980
BsrDI	GCAATG	1 cut(s)	555
BsrFI	RCCGGY	1 cut(s)	311
BsrGI	TGTACA	1 cut(s)	645
BsrI	ACTGG	4 cut(s)	268, 752, 757, 938
BssAI	RCCGGY	1 cut(s)	311
BssECI	CCNNGG	2 cut(s)	176, 716
BssNI	GRCGYC	1 cut(s)	141
BssSI	CACGAG	1 cut(s)	953
BssT1I	CCWWGG	1 cut(s)	176
Bst2BI	CACGAG	1 cut(s)	953
Bst2UI	CCWGG	2 cut(s)	57, 881
Bst4CI	ACNGT	5 cut(s)	398, 466, 773, 1068, 1130
Bst6I	CTCTTC	3 cut(s)	78, 744, 1042
BstACI	GRCGYC	1 cut(s)	141
BstAPI	GCANNNNNTGC	1 cut(s)	290
BstAUI	TGTACA	1 cut(s)	645
BstBI	TTCGAA	1 cut(s)	343
BstDEI	CTNAG	2 cut(s)	45, 104
BstDSI	CCRYGG	2 cut(s)	176, 716
BstEII	GGTNACC	1 cut(s)	1013
BstENI	CCTNNNNNAGG	1 cut(s)	124
BstF5I	GGATG	1 cut(s)	70
BstH2I	RGCGCY	1 cut(s)	392
BstHHI	GCGC	1 cut(s)	391
BstMAI	GTCTC	2 cut(s)	48, 980
BstMCI	CGRYCG	2 cut(s)	316, 949
BstMWI	GCNNNNNNNGC	2 cut(s)	290, 364
BstNI	CCWGG	2 cut(s)	57, 881
BstNSI	RCATGY	1 cut(s)	817
BstPI	GGTNACC	1 cut(s)	1013
BstSCI	CCNGG	3 cut(s)	55, 604, 879
BstSFI	CTRYAG	2 cut(s)	6, 331
BstV1I	GCAGC	4 cut(s)	277, 456, 459, 847
BstV2I	GAAGAC	4 cut(s)	504, 514, 987, 1114
BstXI	CCANNNNNNTGG	1 cut(s)	880
BstZI	CGGCCG	1 cut(s)	313
BsuRI	GGCC	4 cut(s)	308, 315, 975, 1028
BtgI	CCRYGG	2 cut(s)	176, 716
BtgZI	GCGATG	1 cut(s)	1146
BtsCI	GGATG	1 cut(s)	70
BtsIMutI	CAGTG	1 cut(s)	1135
CciI	TCATGA	5 cut(s)	694, 793, 886, 895, 901
CfoI	GCGC	1 cut(s)	391
Cfr10I	RCCGGY	1 cut(s)	311
Cfr13I	GGNCC	1 cut(s)	49
CseI	GACGC	1 cut(s)	373
CsiI	ACCWGGT	1 cut(s)	879
Csp6I	GTAC	3 cut(s)	229, 267, 646
CviQI	GTAC	3 cut(s)	229, 267, 646
DdeI	CTNAG	2 cut(s)	45, 104
EaeI	YGGCCR	2 cut(s)	313, 973
EagI	CGGCCG	1 cut(s)	313
Eam1104I	CTCTTC	3 cut(s)	78, 744, 1042
EarI	CTCTTC	3 cut(s)	78, 744, 1042
EclXI	CGGCCG	1 cut(s)	313
Eco130I	CCWWGG	1 cut(s)	176
Eco24I	GRGCYC	1 cut(s)	560
Eco31I	GGTCTC	1 cut(s)	980
Eco47I	GGWCC	1 cut(s)	49
Eco52I	CGGCCG	1 cut(s)	313
Eco57I	CTGAAG	1 cut(s)	220
Eco88I	CYCGRG	2 cut(s)	170, 722
Eco91I	GGTNACC	1 cut(s)	1013
EcoNI	CCTNNNNNAGG	1 cut(s)	124
EcoO109I	RGGNCCY	1 cut(s)	49
EcoO65I	GGTNACC	1 cut(s)	1013
EcoRII	CCWGG	2 cut(s)	55, 879
EcoT14I	CCWWGG	1 cut(s)	176
EcoT22I	ATGCAT	2 cut(s)	423, 711
EcoT38I	GRGCYC	1 cut(s)	560
ErhI	CCWWGG	1 cut(s)	176
FalI	AAGNNNNNCTT	6 cut(s)	72, 104, 75, 107, 646, 678
FaqI	GGGAC	4 cut(s)	35, 194, 396, 1073
FbaI	TGATCA	3 cut(s)	796, 889, 898
Fnu4HI	GCNGC	5 cut(s)	291, 337, 445, 448, 836
FokI	GGATG	1 cut(s)	57
FriOI	GRGCYC	1 cut(s)	560
Fsp4HI	GCNGC	5 cut(s)	291, 337, 445, 448, 836
GlaI	GCGC	1 cut(s)	390
GluI	GCNGC	5 cut(s)	291, 337, 445, 448, 836
GsuI	CTGGAG	2 cut(s)	39, 1165
HaeII	RGCGCY	1 cut(s)	392
HaeIII	GGCC	4 cut(s)	308, 315, 975, 1028
HapII	CCGG	3 cut(s)	294, 312, 605
HgaI	GACGC	1 cut(s)	373
HhaI	GCGC	1 cut(s)	391
Hin1I	GRCGYC	1 cut(s)	141
Hin6I	GCGC	1 cut(s)	389
HinP1I	GCGC	1 cut(s)	389
HindIII	AAGCTT	1 cut(s)	783
HinfI	GANTC	7 cut(s)	22, 297, 348, 485, 581, 637, 683
HpaII	CCGG	3 cut(s)	294, 312, 605
HphI	GGTGA	1 cut(s)	1025
Hpy188I	TCNGA	6 cut(s)	105, 353, 409, 636, 801, 1183
Hpy99I	CGWCG	3 cut(s)	623, 1058, 1171
HpyAV	CCTTC	4 cut(s)	298, 580, 864, 1056
HpyCH4III	ACNGT	5 cut(s)	398, 466, 773, 1068, 1130
HpyCH4IV	ACGT	2 cut(s)	141, 1087
HpyCH4V	TGCA	7 cut(s)	8, 198, 284, 421, 516, 666, 709
HpyF10VI	GCNNNNNNNGC	2 cut(s)	290, 364
HpyF3I	CTNAG	2 cut(s)	45, 104
HpySE526I	ACGT	2 cut(s)	141, 1087
Hsp92I	GRCGYC	1 cut(s)	141
HspAI	GCGC	1 cut(s)	389
Ksp22I	TGATCA	3 cut(s)	796, 889, 898
LguI	GCTCTTC	1 cut(s)	78
LmnI	GCTCC	1 cut(s)	1146
Lsp1109I	GCAGC	4 cut(s)	277, 456, 459, 847
LweI	GCATC	2 cut(s)	718, 968
MabI	ACCWGGT	1 cut(s)	879
MaeII	ACGT	2 cut(s)	141, 1087
MaeIII	GTNAC	2 cut(s)	317, 1013
MfeI	CAATTG	1 cut(s)	477
MhlI	GDGCHC	1 cut(s)	560
MlsI	TGGCCA	1 cut(s)	975
MluCI	AATT	2 cut(s)	472, 477
MluNI	TGGCCA	1 cut(s)	975
MlyI	GAGTC	3 cut(s)	494, 575, 677
MmeI	TCCRAC	3 cut(s)	387, 884, 897
Mox20I	TGGCCA	1 cut(s)	975
Mph1103I	ATGCAT	2 cut(s)	423, 711
MroXI	GAANNNNTTC	1 cut(s)	87
MscI	TGGCCA	1 cut(s)	975
MseI	TTAA	1 cut(s)	162
MslI	CAYNNNNRTG	1 cut(s)	900
Msp20I	TGGCCA	1 cut(s)	975
MspA1I	CMGCKG	1 cut(s)	336
MspI	CCGG	3 cut(s)	294, 312, 605
MspR9I	CCNGG	3 cut(s)	57, 606, 881
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	1 cut(s)	339
MvaI	CCWGG	2 cut(s)	57, 881
MwoI	GCNNNNNNNGC	2 cut(s)	290, 364
NciI	CCSGG	1 cut(s)	606
NcoI	CCATGG	1 cut(s)	176
NlaIV	GGNNCC	2 cut(s)	51, 208
NmuCI	GTSAC	2 cut(s)	317, 1013
NsiI	ATGCAT	2 cut(s)	423, 711
NspI	RCATGY	1 cut(s)	817
NspV	TTCGAA	1 cut(s)	343
PaeR7I	CTCGAG	2 cut(s)	170, 722
PagI	TCATGA	5 cut(s)	694, 793, 886, 895, 901
PciSI	GCTCTTC	1 cut(s)	78
PcsI	WCGNNNNNNNCGW	4 cut(s)	277, 627, 951, 1169
PctI	GAATGC	1 cut(s)	339
PdmI	GAANNNNTTC	1 cut(s)	87
PfeI	GAWTC	4 cut(s)	22, 297, 348, 637
PkrI	GCNGC	5 cut(s)	292, 338, 446, 449, 837
Ple19I	CGATCG	1 cut(s)	949
PleI	GAGTC	3 cut(s)	493, 575, 677
PpsI	GAGTC	3 cut(s)	493, 575, 677
PpuMI	RGGWCCY	1 cut(s)	49
Psp5II	RGGWCCY	1 cut(s)	49
Psp6I	CCWGG	2 cut(s)	55, 879
PspEI	GGTNACC	1 cut(s)	1013
PspGI	CCWGG	2 cut(s)	55, 879
PspN4I	GGNNCC	2 cut(s)	51, 208
PspPI	GGNCC	1 cut(s)	49
PspPPI	RGGWCCY	1 cut(s)	49
PspXI	VCTCGAGB	1 cut(s)	170
PsrI	GAACNNNNNNTAC	2 cut(s)	212, 244
PstI	CTGCAG	1 cut(s)	10
PvuI	CGATCG	1 cut(s)	949
RsaI	GTAC	3 cut(s)	230, 268, 647
RsaNI	GTAC	3 cut(s)	229, 267, 646
RseI	CAYNNNNRTG	1 cut(s)	900
SapI	GCTCTTC	1 cut(s)	78
SaqAI	TTAA	1 cut(s)	162
SatI	GCNGC	5 cut(s)	291, 337, 445, 448, 836
Sau96I	GGNCC	1 cut(s)	49
SchI	GAGTC	3 cut(s)	494, 575, 677
ScrFI	CCNGG	3 cut(s)	57, 606, 881
SduI	GDGCHC	1 cut(s)	560
SexAI	ACCWGGT	1 cut(s)	879
SfaNI	GCATC	2 cut(s)	718, 968
SfcI	CTRYAG	2 cut(s)	6, 331
Sfr274I	CTCGAG	2 cut(s)	170, 722
SfuI	TTCGAA	1 cut(s)	343
SinI	GGWCC	1 cut(s)	49
SlaI	CTCGAG	2 cut(s)	170, 722
SmiMI	CAYNNNNRTG	1 cut(s)	900
SmlI	CTYRAG	2 cut(s)	170, 722
SmoI	CTYRAG	2 cut(s)	170, 722
Sse9I	AATT	2 cut(s)	472, 477
SsiI	CCGC	2 cut(s)	215, 336
StyD4I	CCNGG	3 cut(s)	55, 604, 879
StyI	CCWWGG	1 cut(s)	176
TaaI	ACNGT	5 cut(s)	398, 466, 773, 1068, 1130
TaiI	ACGT	2 cut(s)	144, 1090
TaqII	GACCGA	1 cut(s)	509
TasI	AATT	2 cut(s)	472, 477
TatI	WGTACW	1 cut(s)	645
TauI	GCSGC	1 cut(s)	339
TfiI	GAWTC	4 cut(s)	22, 297, 348, 637
Tru1I	TTAA	1 cut(s)	162
Tru9I	TTAA	1 cut(s)	162
TscAI	CASTG	1 cut(s)	1135
TseFI	GTSAC	2 cut(s)	317, 1013
TseI	GCWGC	4 cut(s)	290, 444, 447, 835
Tsp45I	GTSAC	2 cut(s)	317, 1013
TspDTI	ATGAA	7 cut(s)	35, 438, 680, 694, 711, 875, 993
TspGWI	ACGGA	1 cut(s)	92
TspRI	CASTG	1 cut(s)	1135
VpaK11BI	GGWCC	1 cut(s)	49
XagI	CCTNNNNNAGG	1 cut(s)	124
XceI	RCATGY	1 cut(s)	817
XhoI	CTCGAG	2 cut(s)	170, 722
XmnI	GAANNNNTTC	1 cut(s)	87
ZraI	GACGTC	1 cut(s)	142
Zsp2I	ATGCAT	2 cut(s)	423, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.