MD06G1121400.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014479
Physical Location & Seq
Reverse (-)
26181095 .. 26183024
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1121400.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGTCAGATGATCAAATGAGTCTATCAATAAATGGTCAATCTCAAGTTCCCCCAGGTTTCCGGTTCCATCCAACCGAAGAGGAGCTTCTTCACTACTATCTTAGGAAGAAAGTTGCCTTTGAGAGGATTGATCTTGATGTAATTCGAGAAGTTGATCTTAATAAGCTTGAGCCATGGGACATTCAAGAGAAGTGCAAAATAGGTTCCACTCCACAAAATGATTGGTACTTCTTCAGTCACAAGGACAAGAAATACCCAACCGGAACTCGAACCAATCGGGCAACCACTGCTGGGTTTTGGAAGGCCACCGGGCGGGACAAGATCATCTATAGCGGCTTCAAAAGAATTGGATTGAGGAAGACACTTGTGTTTTATAAGGGTCGAGCTCCTCATGGACAAAAGTCAGATTGGATCATGCATGAATATAGGCTTGATGAAAGCAACACTCATGAAACCACCGTTTCTAGCTCAATGGGGGAGTCGATGACCGAAGAAGGGTGGGTGGTCTGCCGGGTATTCAAGAAGAAGAACTATCAGAAAGCTTTAGAGAGCCCTAAAGCCTCCTTCTCCATGGACTCATCAAACAACCAAATGCATGGTTCAAGAAACGATGGTGTTCTTGATCAAATACTGATGTACATGGGAAGGACTACTTGCAAGCTGGAAAATCATGATCAATCCTTAACCATGAATAACATCTCAGAAAGATTTATGCATCTGCCAAGGCTTGAGAGCCCAACTCTTCCAAACCTTCCCACTTTCGATCAGGAACGTAGCTTCAAAGCTTGCTATTCGGCCATTGACGACATGTTCATAGAAACTGAGCCTTCTTCAACAAACCAACCAAGCAATGGTTGTCACAATAATGACCTAGTCGATGATCATGAGTACCCCAAAACAAGGCTAAATGACTGGGCTACCCTTGATAGGCTTGTGGCATCCCAACTAGGTCAACTCAATGGCCAAGATCAAGAGACACCAAAACACTTGTCTTGCTTTGGCGATCCAAACATGGCCTTTTGTTCTTCTCCTCATCCTAATGATCAAGACAATGACGTACAGCTATCATATCCATACCTACGTACAAGTAGATCATCCGATCATCAATCCGAAGTATACAACAACGAGAATGATCTGTGGAACTTCACCAAATCGTCGTCATCACCGTCATCATCGGACCCGCTTTGCCACTTGTCGGTGTAA

Protein Analysis

401

Amino Acids

46.02

Weight (kDa)

5.85

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 17 - 144 1.7e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014479)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32770
fragaria_vesca FvH4_5g10690
malus_domestica MD06G1121400.v1.1 MD14G1137900.v1.1
prunus_persica Prupe.5G131900_v2.0.a1
pyrus_communis pycom06g11260 pycom14g11310
rosa_chinensis RchiOBHm_Chr7g0187451
rosa_laevigata RLG00000004780
rosa_multiflora Rmu_sc0001230.1_g000011
rosa_roxburghii Rroxscaffold_3G00267410
rosa_rugosa Rorug06G0481700
rosa_samantha Rh7AG086200 Rh7BG087700 Rh7CG087100 Rh7DG088700
rosa_wichuraiana Rw7G007610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 851
AccI GTMKAC 1 cut(s) 1116
AciI CCGC 3 cut(s) 313, 333, 1181
AclWI GGATC 2 cut(s) 419, 998
AcoI YGGCCR 2 cut(s) 795, 961
AcuI CTGAAG 1 cut(s) 217
AfaI GTAC 5 cut(s) 227, 638, 890, 1059, 1084
AfiI CCNNNNNNNGG 8 cut(s) 123, 291, 312, 495, 851, 900, 927, 1195
AflIII ACRYGT 1 cut(s) 807
AgsI TTSAA 6 cut(s) 185, 340, 520, 603, 781, 834
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 9 cut(s) 85, 166, 386, 468, 542, 661, 777, 785, 1063
AluI AGCT 9 cut(s) 85, 166, 386, 468, 542, 661, 777, 785, 1063
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 2 cut(s) 419, 998
AoxI GGCC 4 cut(s) 303, 795, 961, 1014
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AspS9I GGNCC 1 cut(s) 1177
AsuC2I CCSGG 2 cut(s) 310, 512
AsuHPI GGTGA 2 cut(s) 1138, 1155
AvaII GGWCC 1 cut(s) 1177
BalI TGGCCA 1 cut(s) 963
BanII GRGCYC 3 cut(s) 388, 554, 737
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 388
BccI CCATC 2 cut(s) 75, 605
BciT130I CCWGG 1 cut(s) 54
BclI TGATCA 5 cut(s) 10, 622, 673, 880, 1042
BcnI CCSGG 2 cut(s) 310, 512
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 3 cut(s) 465, 872, 947
BfmI CTRYAG 1 cut(s) 328
BisI GCNGC 1 cut(s) 334
BlsI GCNGC 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 54, 310, 512
Bme18I GGWCC 1 cut(s) 1177
BmgT120I GGNCC 1 cut(s) 1177
BmiI GGNNCC 3 cut(s) 65, 205, 1179
BmrFI CCNGG 3 cut(s) 54, 310, 512
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 2 cut(s) 724, 947
BmuI ACTGGG 1 cut(s) 922
BoxI GACNNNNGTC 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 724, 756
BpuEI CTTGAG 3 cut(s) 27, 188, 749
BpuMI CCSGG 2 cut(s) 310, 512
BsaAI YACGTR 1 cut(s) 1082
BsaJI CCNNGG 4 cut(s) 52, 173, 570, 722
BsaWI WCCGGW 2 cut(s) 60, 260
Bsc4I CCNNNNNNNGG 8 cut(s) 123, 291, 312, 495, 851, 900, 927, 1195
Bse1I ACTGG 1 cut(s) 917
Bse3DI GCAATG 1 cut(s) 856
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 4 cut(s) 52, 173, 570, 722
BseGI GGATG 4 cut(s) 67, 938, 1033, 1094
BseLI CCNNNNNNNGG 8 cut(s) 123, 291, 312, 495, 851, 900, 927, 1195
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 2 cut(s) 714, 813
BseNI ACTGG 1 cut(s) 917
BseRI GAGGAG 3 cut(s) 95, 378, 1020
BseYI CCCAGC 1 cut(s) 290
BshFI GGCC 4 cut(s) 305, 797, 963, 1016
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 4 cut(s) 61, 261, 309, 511
BslFI GGGAC 2 cut(s) 191, 329
BslI CCNNNNNNNGG 8 cut(s) 123, 291, 312, 495, 851, 900, 927, 1195
BsmAI GTCTC 1 cut(s) 968
BsmFI GGGAC 2 cut(s) 191, 329
BsnI GGCC 4 cut(s) 305, 797, 963, 1016
Bsp1286I GDGCHC 3 cut(s) 388, 554, 737
Bsp1407I TGTACA 1 cut(s) 636
Bsp19I CCATGG 2 cut(s) 173, 570
BspACI CCGC 3 cut(s) 313, 333, 1181
BspANI GGCC 4 cut(s) 305, 797, 963, 1016
BspCNI CTCAG 2 cut(s) 713, 814
BspHI TCATGA 3 cut(s) 448, 670, 883
BspLI GGNNCC 3 cut(s) 65, 205, 1179
BspPI GGATC 2 cut(s) 419, 998
BsrDI GCAATG 1 cut(s) 856
BsrGI TGTACA 1 cut(s) 636
BsrI ACTGG 1 cut(s) 917
BssECI CCNNGG 4 cut(s) 52, 173, 570, 722
BssNAI GTATAC 1 cut(s) 1117
BssT1I CCWWGG 3 cut(s) 173, 570, 722
Bst1107I GTATAC 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 460, 1167
Bst6I CTCTTC 2 cut(s) 72, 747
BstAPI GCANNNNNTGC 1 cut(s) 287
BstAUI TGTACA 1 cut(s) 636
BstBAI YACGTR 1 cut(s) 1082
BstC8I GCNNGC 2 cut(s) 659, 787
BstDEI CTNAG 3 cut(s) 101, 700, 822
BstDSI CCRYGG 2 cut(s) 173, 570
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 4 cut(s) 67, 938, 1033, 1094
BstMAI GTCTC 1 cut(s) 968
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 811
BstPAI GACNNNNGTC 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 52, 308, 510
BstSFI CTRYAG 1 cut(s) 328
BstSNI TACGTA 1 cut(s) 1082
BstV2I GAAGAC 1 cut(s) 365
BstXI CCANNNNNNTGG 1 cut(s) 596
BstZ17I GTATAC 1 cut(s) 1117
BsuRI GGCC 4 cut(s) 305, 797, 963, 1016
BtgI CCRYGG 2 cut(s) 173, 570
BtsCI GGATG 4 cut(s) 67, 938, 1033, 1094
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 659, 787
CciI TCATGA 3 cut(s) 448, 670, 883
Cfr13I GGNCC 1 cut(s) 1177
Csp6I GTAC 5 cut(s) 226, 637, 889, 1058, 1083
CviQI GTAC 5 cut(s) 226, 637, 889, 1058, 1083
DdeI CTNAG 3 cut(s) 101, 700, 822
EaeI YGGCCR 2 cut(s) 795, 961
Eam1104I CTCTTC 2 cut(s) 72, 747
EarI CTCTTC 2 cut(s) 72, 747
Ecl136II GAGCTC 1 cut(s) 386
Eco105I TACGTA 1 cut(s) 1082
Eco130I CCWWGG 3 cut(s) 173, 570, 722
Eco24I GRGCYC 3 cut(s) 388, 554, 737
Eco47I GGWCC 1 cut(s) 1177
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 386
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 3 cut(s) 173, 570, 722
EcoT22I ATGCAT 3 cut(s) 420, 597, 717
EcoT38I GRGCYC 3 cut(s) 388, 554, 737
ErhI CCWWGG 3 cut(s) 173, 570, 722
FalI AAGNNNNNCTT 6 cut(s) 69, 101, 141, 173, 637, 669
FaqI GGGAC 2 cut(s) 191, 329
FauI CCCGC 2 cut(s) 306, 1188
FbaI TGATCA 5 cut(s) 10, 622, 673, 880, 1042
FblI GTMKAC 1 cut(s) 1116
Fnu4HI GCNGC 1 cut(s) 334
FokI GGATG 4 cut(s) 54, 925, 1020, 1081
FriOI GRGCYC 3 cut(s) 388, 554, 737
Fsp4HI GCNGC 1 cut(s) 334
FspBI CTAG 3 cut(s) 465, 872, 947
GluI GCNGC 1 cut(s) 334
GsaI CCCAGC 1 cut(s) 294
HaeIII GGCC 4 cut(s) 305, 797, 963, 1016
HapII CCGG 4 cut(s) 61, 261, 309, 511
HincII GTYRAC 1 cut(s) 953
HindII GTYRAC 1 cut(s) 953
HindIII AAGCTT 3 cut(s) 164, 540, 783
HinfI GANTC 3 cut(s) 19, 479, 575
HpaII CCGG 4 cut(s) 61, 261, 309, 511
HphI GGTGA 2 cut(s) 1138, 1155
Hpy166II GTNNAC 2 cut(s) 953, 1117
Hpy188I TCNGA 7 cut(s) 7, 406, 537, 703, 1099, 1111, 1177
Hpy8I GTNNAC 2 cut(s) 953, 1117
Hpy99I CGWCG 1 cut(s) 1159
HpyAV CCTTC 6 cut(s) 295, 488, 574, 639, 761, 837
HpyCH4III ACNGT 2 cut(s) 460, 1167
HpyCH4IV ACGT 3 cut(s) 772, 1056, 1081
HpyCH4V TGCA 5 cut(s) 195, 418, 595, 657, 715
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 3 cut(s) 101, 700, 822
HpySE526I ACGT 3 cut(s) 772, 1056, 1081
Ksp22I TGATCA 5 cut(s) 10, 622, 673, 880, 1042
LmnI GCTCC 2 cut(s) 82, 391
LweI GCATC 2 cut(s) 724, 947
MaeI CTAG 3 cut(s) 465, 872, 947
MaeII ACGT 3 cut(s) 772, 1056, 1081
MaeIII GTNAC 2 cut(s) 236, 857
MhlI GDGCHC 3 cut(s) 388, 554, 737
MlsI TGGCCA 1 cut(s) 963
MluCI AATT 2 cut(s) 141, 345
MluNI TGGCCA 1 cut(s) 963
MlyI GAGTC 3 cut(s) 28, 488, 569
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 6 cut(s) 73, 117, 348, 399, 571, 1041
Mox20I TGGCCA 1 cut(s) 963
Mph1103I ATGCAT 3 cut(s) 420, 597, 717
MscI TGGCCA 1 cut(s) 963
MseI TTAA 2 cut(s) 159, 683
MslI CAYNNNNRTG 2 cut(s) 864, 1038
Msp20I TGGCCA 1 cut(s) 963
MspI CCGG 4 cut(s) 61, 261, 309, 511
MspR9I CCNGG 3 cut(s) 54, 310, 512
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 2 cut(s) 310, 512
NcoI CCATGG 2 cut(s) 173, 570
NlaIV GGNNCC 3 cut(s) 65, 205, 1179
NmuCI GTSAC 2 cut(s) 236, 857
NsiI ATGCAT 3 cut(s) 420, 597, 717
NspI RCATGY 1 cut(s) 811
PagI TCATGA 3 cut(s) 448, 670, 883
PciI ACATGT 1 cut(s) 807
PcsI WCGNNNNNNNCGW 1 cut(s) 1163
PflFI GACNNNGTC 1 cut(s) 872
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 1 cut(s) 335
PleI GAGTC 3 cut(s) 27, 487, 569
PpsI GAGTC 3 cut(s) 27, 487, 569
Ppu21I YACGTR 1 cut(s) 1082
PscI ACATGT 1 cut(s) 807
PshAI GACNNNNGTC 1 cut(s) 400
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 65, 205, 1179
PspPI GGNCC 1 cut(s) 1177
PsyI GACNNNGTC 1 cut(s) 872
RsaI GTAC 5 cut(s) 227, 638, 890, 1059, 1084
RsaNI GTAC 5 cut(s) 226, 637, 889, 1058, 1083
RseI CAYNNNNRTG 2 cut(s) 864, 1038
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 2 cut(s) 159, 683
SatI GCNGC 1 cut(s) 334
Sau96I GGNCC 1 cut(s) 1177
SchI GAGTC 3 cut(s) 28, 488, 569
ScrFI CCNGG 3 cut(s) 54, 310, 512
SduI GDGCHC 3 cut(s) 388, 554, 737
SfaNI GCATC 2 cut(s) 724, 947
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 1177
SmiMI CAYNNNNRTG 2 cut(s) 864, 1038
SmlI CTYRAG 3 cut(s) 42, 167, 728
SmoI CTYRAG 3 cut(s) 42, 167, 728
SnaBI TACGTA 1 cut(s) 1082
Sse9I AATT 2 cut(s) 141, 345
SsiI CCGC 3 cut(s) 313, 333, 1181
SspMI CTAG 3 cut(s) 465, 872, 947
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 3 cut(s) 52, 308, 510
StyI CCWWGG 3 cut(s) 173, 570, 722
TaaI ACNGT 2 cut(s) 460, 1167
TaiI ACGT 3 cut(s) 775, 1059, 1084
TaqI TCGA 6 cut(s) 145, 268, 382, 482, 762, 876
TaqII GACCGA 1 cut(s) 503
TasI AATT 2 cut(s) 141, 345
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 336
Tru1I TTAA 2 cut(s) 159, 683
Tru9I TTAA 2 cut(s) 159, 683
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 2 cut(s) 236, 857
Tsp45I GTSAC 2 cut(s) 236, 857
TspDTI ATGAA 5 cut(s) 435, 450, 465, 704, 802
TspRI CASTG 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 872
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 1177
XagI CCTNNNNNAGG 1 cut(s) 121
XceI RCATGY 1 cut(s) 811
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 848
XmiI GTMKAC 1 cut(s) 1116
XspI CTAG 3 cut(s) 465, 872, 947
Zsp2I ATGCAT 3 cut(s) 420, 597, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.