FvH4_5g11730

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011561
Physical Location & Seq
Reverse (-)
6652846 .. 6658265
5420 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11730.t3

Sequence Viewer

Length: 1566 bp
ATGCTCTTGATGTCATCGAAAGTATTGGTTCTTAGCCAGCATGTTCTGTTTTATCAAGAATGTTACCCGTTAAGCAATGCTGGGACTTACCCCCAAAATAGTCCAGTAAAATTTCGAGGGGATTCAGGATTGCAAGATGGGAATTCAACCGGTAACCACGTGAATCTTGTGGGCGGTTTCTATGATTCCGGCAACAACATAAAGTTCAGTTTCTCTACAGCTTATACCATGACCTTGTTGAGTTGGACTGTAATTGAATACCACCAAAAGTATGCTGATATTGGTGAGCTTGACCATGTTAAGGACATAATCAAATGGGGCAGTGATTATTTGCTCAAAGTCTTTGTCCCTCCAAATGCAACTTCAGATACCACATTGTATTCTCAGATTGGAAATGCAAACAGTGATGGCACGACTCCTAGTGATATAAACTGCTGGGAAAGACCTGAAGACATGGATTATGTGAGACCTGTTTCAATTTGTGATAGCACAGCTTCCGATTTAGCAGGGGAGATTGTTGCAGCATTATCTGCTGCTTCATTAGTATTTAAAAGCGATGACAGTGTATACTCGGGAGAATTAGTGAAAGCAGCTGGGGAGCTATTTGAAAGTGCAACTAAGGACCCTAATAATCAAGGAACTTACACCAGGGATGAAAGTTGTGGAGGACAATCTAGAGACTTTTATAACTCATCTGGTTATAATGATGAACTAGTTTGGGGAGGAACTTGGTTATTTTTTGCTACTGGTGACATTTCATATCTGCAATATGCCACGGACCTGTTTCAGGCTGCAGTTGATAGTGAAACAAGTTCTGATAAATGGATTTTCGATTGGAACAACAAGCTTACTGCTAACATGGTCTTGCTAACGCGTCTTCAATTCTTTCATGATCTTGGCTACCCCTATGAAGGTTCTTTAATGACATCGGCCAACATGACTGGCTCCCTTATGTGTTCTTATGTTTCTAGTCGAATCTTCAACATGACACCAGGGGGATTGGTCATCCAAAGGCCTAATTATGGGGCACCTCTCCAGTTTGCTGCAACAGCGTCTTTCCTTAGTAAATTGTACAGCGACTACCTTGATCTCCTTCTTAGTTCTGGTGGGAGTTGCAGCACTGGTGAATTTACCGTAGACATGCTGCGCAATTTCTCCTTGTCTCAGGTGAATTACATTCTAGGAGATAATCCAATGAATATGAGCTACATGGTGGGGTTTGGAGACAAGTTTCCGAGCCAAGTACACCATAGAAGTGCATCAATCCCTTGGGATGGTCAGCATTACTTGTGTAAAGATGGATATAAATGGCGAGACTCTAAGGATCCAAATCCGAATGTGCTGTTGGGAGCCATGGTAGGAGGACCAGACGAGTTTGACAAATTTATAGATGAGAGGGATAGACAAGAGTTCACTGAGCCTAGAATAACAAGCAATGCTGGTTTGGTTGCAGCTCTCATTGCACTTCATGATCCTCCTCATACTAGGAAGAACAAGAAGAAAGGCCATTATGATCCCACTAAATTGGGCATAGACCAGATGGGGATTTTTGATAAGATTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

522

Amino Acids

57.81

Weight (kDa)

4.86

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 116 - 588 8.8e-127 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 687, 702
Acc16I TGCGCA 1 cut(s) 1148
AccB1I GGYRCC 1 cut(s) 1027
AccI GTMKAC 2 cut(s) 567, 1137
AccII CGCG 1 cut(s) 874
AciI CCGC 1 cut(s) 174
AclWI GGATC 4 cut(s) 1319, 1332, 1466, 1508
AcoI YGGCCR 1 cut(s) 930
AcsI RAATTY 4 cut(s) 110, 142, 1127, 1382
AcuI CTGAAG 2 cut(s) 348, 468
AcvI CACGTG 1 cut(s) 160
AfaI GTAC 2 cut(s) 1073, 1245
AfiI CCNNNNNNNGG 5 cut(s) 301, 787, 911, 1022, 1274
AflIII ACRYGT 1 cut(s) 872
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 6 cut(s) 147, 257, 477, 608, 881, 982
AhlI ACTAGT 1 cut(s) 712
AjnI CCWGG 2 cut(s) 647, 991
AjuI GAANNNNNNNTTGG 4 cut(s) 346, 378, 1328, 1360
AluBI AGCT 8 cut(s) 221, 289, 494, 593, 601, 847, 1206, 1454
AluI AGCT 8 cut(s) 221, 289, 494, 593, 601, 847, 1206, 1454
Alw26I GTCTC 5 cut(s) 460, 672, 1167, 1218, 1308
AlwI GGATC 4 cut(s) 1319, 1332, 1466, 1508
Ama87I CYCGRG 1 cut(s) 571
AoxI GGCC 3 cut(s) 930, 1013, 1504
ApeKI GCWGC 8 cut(s) 521, 533, 590, 791, 1043, 1116, 1144, 1451
ApoI RAATTY 4 cut(s) 110, 142, 1127, 1382
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 3 cut(s) 622, 778, 1364
AsuHPI GGTGA 4 cut(s) 296, 761, 1136, 1180
AvaI CYCGRG 1 cut(s) 571
AvaII GGWCC 3 cut(s) 622, 778, 1364
BaeGI GKGCMC 1 cut(s) 1030
BamHI GGATCC 1 cut(s) 1324
BanI GGYRCC 1 cut(s) 1027
BbrPI CACGTG 1 cut(s) 160
BbsI GAAGAC 2 cut(s) 456, 869
BbvI GCAGC 8 cut(s) 520, 533, 602, 778, 1030, 1128, 1131, 1463
BccI CCATC 5 cut(s) 131, 401, 1268, 1292, 1534
BciT130I CCWGG 2 cut(s) 649, 993
BcoDI GTCTC 5 cut(s) 460, 672, 1167, 1218, 1308
BcuI ACTAGT 1 cut(s) 712
BfaI CTAG 7 cut(s) 420, 675, 713, 969, 1181, 1422, 1485
BfmI CTRYAG 2 cut(s) 216, 792
BisI GCNGC 8 cut(s) 522, 534, 591, 792, 1044, 1117, 1145, 1452
BlsI GCNGC 8 cut(s) 523, 535, 592, 793, 1045, 1118, 1146, 1453
Bme1390I CCNGG 2 cut(s) 649, 993
Bme18I GGWCC 3 cut(s) 622, 778, 1364
BmeT110I CYCGRG 1 cut(s) 571
BmgT120I GGNCC 3 cut(s) 622, 778, 1364
BmiI GGNNCC 5 cut(s) 624, 946, 1029, 1326, 1351
BmrFI CCNGG 2 cut(s) 649, 993
BmsI GCATC 1 cut(s) 1268
BpiI GAAGAC 2 cut(s) 456, 869
BpmI CTGGAG 1 cut(s) 1019
BsaAI YACGTR 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 1329
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 5 cut(s) 648, 774, 992, 1268, 1353
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 4 cut(s) 567, 597, 1341, 1371
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 787, 911, 1022, 1274
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 5 cut(s) 104, 751, 946, 1036, 1126
Bse3DI GCAATG 3 cut(s) 82, 1441, 1458
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 2 cut(s) 649, 993
BseDI CCNNGG 5 cut(s) 648, 774, 992, 1268, 1353
BseGI GGATG 3 cut(s) 658, 1005, 1279
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 5 cut(s) 301, 787, 911, 1022, 1274
BseMI GCAATG 3 cut(s) 82, 1441, 1458
BseMII CTCAG 3 cut(s) 398, 1178, 1407
BseNI ACTGG 5 cut(s) 104, 751, 946, 1036, 1126
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 8 cut(s) 520, 533, 602, 778, 1030, 1128, 1131, 1463
BseYI CCCAGC 3 cut(s) 80, 435, 593
Bsh1236I CGCG 1 cut(s) 874
BshFI GGCC 3 cut(s) 932, 1015, 1506
BshNI GGYRCC 1 cut(s) 1027
BshTI ACCGGT 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 150, 189
BslFI GGGAC 2 cut(s) 97, 332
BslI CCNNNNNNNGG 5 cut(s) 301, 787, 911, 1022, 1274
BsmAI GTCTC 5 cut(s) 460, 672, 1167, 1218, 1308
BsmFI GGGAC 2 cut(s) 97, 332
BsnI GGCC 3 cut(s) 932, 1015, 1506
Bso31I GGTCTC 1 cut(s) 460
BsoBI CYCGRG 1 cut(s) 571
Bsp1286I GDGCHC 1 cut(s) 1030
Bsp1407I TGTACA 1 cut(s) 1071
Bsp143I GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
Bsp19I CCATGG 1 cut(s) 1353
BspACI CCGC 1 cut(s) 174
BspANI GGCC 3 cut(s) 932, 1015, 1506
BspCNI CTCAG 3 cut(s) 397, 1177, 1408
BspFNI CGCG 1 cut(s) 874
BspHI TCATGA 2 cut(s) 889, 1468
BspLI GGNNCC 5 cut(s) 624, 946, 1029, 1326, 1351
BspMAI CTGCAG 1 cut(s) 796
BspPI GGATC 4 cut(s) 1319, 1332, 1466, 1508
BspT107I GGYRCC 1 cut(s) 1027
BspTNI GGTCTC 1 cut(s) 460
BsrDI GCAATG 3 cut(s) 82, 1441, 1458
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 1071
BsrI ACTGG 5 cut(s) 104, 751, 946, 1036, 1126
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 648, 774, 992, 1268, 1353
BssMI GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
BssNAI GTATAC 1 cut(s) 568
BssT1I CCWWGG 2 cut(s) 1268, 1353
Bst1107I GTATAC 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 649, 993
Bst4CI ACNGT 4 cut(s) 250, 404, 563, 1135
BstAPI GCANNNNNTGC 1 cut(s) 530
BstAUI TGTACA 1 cut(s) 1071
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 8 cut(s) 32, 384, 618, 1061, 1097, 1164, 1320, 1416
BstDSI CCRYGG 2 cut(s) 774, 1353
BstEII GGTNACC 1 cut(s) 152
BstENI CCTNNNNNAGG 1 cut(s) 785
BstF5I GGATG 3 cut(s) 658, 1005, 1279
BstFNI CGCG 1 cut(s) 874
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 5 cut(s) 895, 1090, 1327, 1474, 1516
BstMAI GTCTC 5 cut(s) 460, 672, 1167, 1218, 1308
BstMBI GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
BstMWI GCNNNNNNNGC 3 cut(s) 530, 1049, 1460
BstNI CCWGG 2 cut(s) 649, 993
BstNSI RCATGY 2 cut(s) 44, 1144
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 647, 991
BstSFI CTRYAG 2 cut(s) 216, 792
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 8 cut(s) 520, 533, 602, 778, 1030, 1128, 1131, 1463
BstV2I GAAGAC 2 cut(s) 456, 869
BstX2I RGATCY 1 cut(s) 1324
BstXI CCANNNNNNTGG 1 cut(s) 1525
BstYI RGATCY 1 cut(s) 1324
BstZ17I GTATAC 1 cut(s) 568
BsuRI GGCC 3 cut(s) 932, 1015, 1506
BtgI CCRYGG 2 cut(s) 774, 1353
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 3 cut(s) 658, 1005, 1279
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 5 cut(s) 328, 409, 568, 1119, 1413
Cac8I GCNNGC 1 cut(s) 38
CciI TCATGA 2 cut(s) 889, 1468
CfoI GCGC 1 cut(s) 1149
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 3 cut(s) 622, 778, 1364
CseI GACGC 2 cut(s) 863, 1041
Csp6I GTAC 2 cut(s) 1072, 1244
CspAI ACCGGT 1 cut(s) 149
CviQI GTAC 2 cut(s) 1072, 1244
DdeI CTNAG 8 cut(s) 32, 384, 618, 1061, 1097, 1164, 1320, 1416
DpnI GATC 5 cut(s) 894, 1089, 1326, 1473, 1515
DpnII GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
DraI TTTAAA 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 930
Eco130I CCWWGG 2 cut(s) 1268, 1353
Eco147I AGGCCT 1 cut(s) 1015
Eco31I GGTCTC 1 cut(s) 460
Eco47I GGWCC 3 cut(s) 622, 778, 1364
Eco57I CTGAAG 2 cut(s) 348, 468
Eco72I CACGTG 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 571
Eco91I GGTNACC 1 cut(s) 152
EcoNI CCTNNNNNAGG 1 cut(s) 785
EcoO109I RGGNCCY 1 cut(s) 622
EcoO65I GGTNACC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 647, 991
EcoT14I CCWWGG 2 cut(s) 1268, 1353
ErhI CCWWGG 2 cut(s) 1268, 1353
FaqI GGGAC 2 cut(s) 97, 332
FblI GTMKAC 2 cut(s) 567, 1137
Fnu4HI GCNGC 8 cut(s) 522, 534, 591, 792, 1044, 1117, 1145, 1452
FokI GGATG 3 cut(s) 665, 992, 1286
Fsp4HI GCNGC 8 cut(s) 522, 534, 591, 792, 1044, 1117, 1145, 1452
FspBI CTAG 7 cut(s) 420, 675, 713, 969, 1181, 1422, 1485
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 1 cut(s) 1148
GluI GCNGC 8 cut(s) 522, 534, 591, 792, 1044, 1117, 1145, 1452
GsaI CCCAGC 3 cut(s) 84, 439, 597
GsuI CTGGAG 1 cut(s) 1019
HaeIII GGCC 3 cut(s) 932, 1015, 1506
HapII CCGG 2 cut(s) 150, 189
HgaI GACGC 2 cut(s) 863, 1041
HhaI GCGC 1 cut(s) 1149
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 7 cut(s) 122, 163, 185, 415, 975, 1316, 1558
HpaII CCGG 2 cut(s) 150, 189
HphI GGTGA 4 cut(s) 296, 761, 1136, 1180
Hpy166II GTNNAC 4 cut(s) 568, 1138, 1246, 1413
Hpy188I TCNGA 6 cut(s) 367, 387, 499, 817, 1236, 1335
Hpy188III TCNNGA 7 cut(s) 7, 56, 126, 573, 675, 890, 1469
Hpy8I GTNNAC 4 cut(s) 568, 1138, 1246, 1413
HpyAV CCTTC 2 cut(s) 905, 1103
HpyCH4III ACNGT 4 cut(s) 250, 404, 563, 1135
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 3 cut(s) 530, 1049, 1460
HpyF3I CTNAG 8 cut(s) 32, 384, 618, 1061, 1097, 1164, 1320, 1416
HpySE526I ACGT 1 cut(s) 159
HspAI GCGC 1 cut(s) 1147
Kzo9I GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
LmnI GCTCC 3 cut(s) 598, 950, 1349
Lsp1109I GCAGC 8 cut(s) 520, 533, 602, 778, 1030, 1128, 1131, 1463
LweI GCATC 1 cut(s) 1268
MaeI CTAG 7 cut(s) 420, 675, 713, 969, 1181, 1422, 1485
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 62, 152, 749
MalI GATC 5 cut(s) 894, 1089, 1326, 1473, 1515
MboI GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
MboII GAAGA 5 cut(s) 461, 869, 970, 1501, 1510
MflI RGATCY 1 cut(s) 1324
MhlI GDGCHC 1 cut(s) 1030
MluI ACGCGT 1 cut(s) 872
MlyI GAGTC 2 cut(s) 409, 1310
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 9 cut(s) 110, 360, 659, 716, 1041, 1355, 1389, 1485, 1488
MseI TTAA 4 cut(s) 71, 300, 549, 920
MslI CAYNNNNRTG 1 cut(s) 1254
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 2 cut(s) 150, 189
MspR9I CCNGG 2 cut(s) 649, 993
MvaI CCWGG 2 cut(s) 649, 993
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 3 cut(s) 530, 1049, 1460
NcoI CCATGG 1 cut(s) 1353
NdeII GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
NlaIV GGNNCC 5 cut(s) 624, 946, 1029, 1326, 1351
NmuCI GTSAC 1 cut(s) 749
NsbI TGCGCA 1 cut(s) 1148
NspI RCATGY 2 cut(s) 44, 1144
PagI TCATGA 2 cut(s) 889, 1468
PceI AGGCCT 1 cut(s) 1015
PfeI GAWTC 5 cut(s) 122, 163, 185, 975, 1558
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 8 cut(s) 523, 535, 592, 793, 1045, 1118, 1146, 1453
PleI GAGTC 2 cut(s) 409, 1310
PmaCI CACGTG 1 cut(s) 160
PmlI CACGTG 1 cut(s) 160
PpsI GAGTC 2 cut(s) 409, 1310
Ppu21I YACGTR 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 622
PsiI TTATAA 2 cut(s) 687, 702
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 647, 991
PspCI CACGTG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 152
PspFI CCCAGC 3 cut(s) 80, 435, 593
PspGI CCWGG 2 cut(s) 647, 991
PspN4I GGNNCC 5 cut(s) 624, 946, 1029, 1326, 1351
PspPI GGNCC 3 cut(s) 622, 778, 1364
PspPPI RGGWCCY 1 cut(s) 622
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 1 cut(s) 1324
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 2 cut(s) 1073, 1245
RsaNI GTAC 2 cut(s) 1072, 1244
RseI CAYNNNNRTG 1 cut(s) 1254
SaqAI TTAA 4 cut(s) 71, 300, 549, 920
SatI GCNGC 8 cut(s) 522, 534, 591, 792, 1044, 1117, 1145, 1452
Sau3AI GATC 5 cut(s) 892, 1087, 1324, 1471, 1513
Sau96I GGNCC 3 cut(s) 622, 778, 1364
SchI GAGTC 2 cut(s) 409, 1310
ScrFI CCNGG 2 cut(s) 649, 993
SduI GDGCHC 1 cut(s) 1030
SfaNI GCATC 1 cut(s) 1268
SfcI CTRYAG 2 cut(s) 216, 792
SinI GGWCC 3 cut(s) 622, 778, 1364
SmiMI CAYNNNNRTG 1 cut(s) 1254
SpeI ACTAGT 1 cut(s) 712
SseBI AGGCCT 1 cut(s) 1015
SsiI CCGC 1 cut(s) 174
SspMI CTAG 7 cut(s) 420, 675, 713, 969, 1181, 1422, 1485
StuI AGGCCT 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 647, 991
StyI CCWWGG 2 cut(s) 1268, 1353
TaaI ACNGT 4 cut(s) 250, 404, 563, 1135
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 17, 115, 831, 973
TatI WGTACW 2 cut(s) 1071, 1243
TfiI GAWTC 5 cut(s) 122, 163, 185, 975, 1558
Tru1I TTAA 4 cut(s) 71, 300, 549, 920
Tru9I TTAA 4 cut(s) 71, 300, 549, 920
TscAI CASTG 5 cut(s) 328, 409, 568, 1126, 1420
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 8 cut(s) 521, 533, 590, 791, 1043, 1116, 1144, 1451
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 8 cut(s) 528, 669, 723, 747, 878, 924, 1211, 1457
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 5 cut(s) 328, 409, 568, 1126, 1420
VpaK11BI GGWCC 3 cut(s) 622, 778, 1364
XagI CCTNNNNNAGG 1 cut(s) 785
XapI RAATTY 4 cut(s) 110, 142, 1127, 1382
XbaI TCTAGA 1 cut(s) 674
XceI RCATGY 2 cut(s) 44, 1144
XmiI GTMKAC 2 cut(s) 567, 1137
XspI CTAG 7 cut(s) 420, 675, 713, 969, 1181, 1422, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.