RchiOBHm_Chr7g0186031

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011561
Physical Location & Seq
Forward (+)
6043755 .. 6046180
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16607

Sequence Viewer

Length: 1821 bp
ATGCCATCTTCACTAGGTTACCAGTCAACTGCAACAGACCAAGAATCCGTGATGTTTGTCCATACAGTTTCAGAGGCACGTCGTATGCTTCCTTCAGCAAGTCGCTGGAACTCTATAGAAATTGACTTCAATCTTGTTCCTCAATCTGGCATCGCCTATGATTCACTGCCTTCTAGTTACTCCAAGTCGTATGATTACGATATTGTTATTAGAGATAGAAAGATCTTCAAACGTTGTCTTTACATTTCTGCCTCAATAGTTAGTCTTATTGTAGCTCTAATTTTGCTGCTACGGTTTTTGCCTCACAAGCATCATCACCATGGTCCTTCAAAAAATCTGACACTTGCATTGAACCAAGCTCTAACCTTCTTCGATGCTCAAAAATGTAATCTACCATCACCATTATACACTGGGACTTACCCCAAAAATAGTCCAGTAAAATTTCGAGGGGATTCAGGATTGCAAGATGGGAATTTGACCAGTAACCACGCAAATCTTGTGGGCGGTTTCTATGATTCTGGAAACAACATGAAGTTCAGTTTCTCTACAGCTTATACCATGACCTTGTTAAGTTGGACTGTGATTGAATACCACCAAAAATATGCTGATATCAGTGAGCTTGACCATGTCAAGGATATAATCAAATGGGGCAGTGATTATTTGCTCAAAGTCTTCATTGGAAGTGCAAACAGTGATGGCACGACTCCTAGTGATATAAACTGCTGGGAAAGACCTGAAGACATGGATTATCAGAGACCTGTTTCAATTTGTGATAGCACAGCTACCGATTTAGCAGGGGAAATTGTTGCAGCATTATCTGCTGCTTCATTAGTATTTAAAGACGACGACAGTGTTTACTCAGGAGAATTAGTGAAAGCTGCTGGGGAGCTATTTGAGAGTGCAACTAAAGACCCTAATAATCAAGGAACTTACACCAGGGTTGAAACTTGTGGAGGAGAATCTAGAGACTTTTATAACTCATCTGGTTATAAAGACGAATTAGTTTGGGGAGGAACTTGGTTATTTTTTGCTACCGGTGACATTTCATATCTGCAATATGCCACAGACAAGTTTCAGGCTGCAGTTGATGATGAAACAAGTTCTGATAAATGGATTTTCGATTGGAACAACAAGCTTACTGCTAACATGGTGTTGCTAACGCGTCTTCAATTCTTTCATGATCTTGGCTACCCGTATGAAGCTTCTTTAAAGGCATCATCCAACATGACTGGCTCGCTTATGTGTTCTTATGTTTCTCGTCGAGTCTTCAGCATGACACCAGGTGGGTTGGTCATCCAAAGGCCTGATTATGGGGCACCTCTCCAGTTTGCTGCAACAGCATCTTTCCTTAGTAAATTGTACGGCGACTACCTTGATCTTCTGCGTAGTTCTGGTGGCAGTGGCAGCACTTGTGAATTTACCGTGAATTACATTCTAGGAGATAACCCAATGAATATGAGCTACATTGTGGGCTTCGGGGACAAGTTTCCGAGCCAAGTACACCATAGAAGTGCATCAATCCTTTGGGATGGTCAACATTACTCGTGTAAAGATGGATATAAATGGCGAGACTCCAAGGATCCAAATCCGAATGTGCTGTTGGGAGCCATGGTAGGAGGACCAGACCAGTTTGACAAATTTATAGATGAGAGGGACAAGCAAGAGTTCACTGAGCCTAGATTAACAAGCAATGCTGGTTTAGTTGCAGCTCTCATTGCACTTCATGATCCTCCTCATACTAAGAAGAGCAAGAAGAGAGGCAACTATGATCCCACTAAATTGGGCATAGACCAGATGGGTATTTTTGATAAGATTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

606

Amino Acids

67.57

Weight (kDa)

5.62

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 116 - 571 5.4e-115 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 975, 990
AccB1I GGYRCC 1 cut(s) 1315
AccII CGCG 1 cut(s) 1162
AciI CCGC 1 cut(s) 504
AclI AACGTT 1 cut(s) 232
AclWI GGATC 4 cut(s) 1574, 1587, 1721, 1763
AcsI RAATTY 4 cut(s) 440, 472, 1415, 1637
AcuI CTGAAG 3 cut(s) 78, 756, 1252
AdeI CACNNNGTG 1 cut(s) 1283
AfaI GTAC 2 cut(s) 1361, 1500
AfiI CCNNNNNNNGG 3 cut(s) 146, 631, 1310
AflIII ACRYGT 1 cut(s) 1160
AgeI ACCGGT 1 cut(s) 1034
AgsI TTSAA 8 cut(s) 130, 229, 330, 352, 587, 765, 944, 1169
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 2 cut(s) 935, 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 1583, 1615
Alw26I GTCTC 3 cut(s) 748, 960, 1563
AlwI GGATC 4 cut(s) 1574, 1587, 1721, 1763
AoxI GGCC 1 cut(s) 1301
ApeKI GCWGC 8 cut(s) 286, 809, 821, 878, 1079, 1331, 1404, 1706
ApoI RAATTY 4 cut(s) 440, 472, 1415, 1637
AsiGI ACCGGT 1 cut(s) 1034
AspS9I GGNCC 2 cut(s) 323, 1619
AsuHPI GGTGA 3 cut(s) 308, 390, 1049
AvaII GGWCC 2 cut(s) 323, 1619
BaeGI GKGCMC 1 cut(s) 1318
BamHI GGATCC 1 cut(s) 1579
BanI GGYRCC 1 cut(s) 1315
BarI GAAGNNNNNNTAC 2 cut(s) 76, 108
BauI CACGAG 1 cut(s) 1543
BbsI GAAGAC 4 cut(s) 664, 744, 1157, 1258
BbvI GCAGC 8 cut(s) 273, 808, 821, 865, 1066, 1318, 1416, 1718
BccI CCATC 7 cut(s) 13, 403, 461, 689, 1523, 1547, 1789
BceAI ACGGC 1 cut(s) 1378
BciT130I CCWGG 2 cut(s) 937, 1281
BcoDI GTCTC 3 cut(s) 748, 960, 1563
BfaI CTAG 6 cut(s) 14, 174, 708, 963, 1436, 1677
BfmI CTRYAG 3 cut(s) 114, 546, 1080
BglII AGATCT 1 cut(s) 222
BisI GCNGC 8 cut(s) 287, 810, 822, 879, 1080, 1332, 1405, 1707
BlsI GCNGC 8 cut(s) 288, 811, 823, 880, 1081, 1333, 1406, 1708
Bme1390I CCNGG 2 cut(s) 937, 1281
Bme18I GGWCC 2 cut(s) 323, 1619
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 323, 1619
BmiI GGNNCC 3 cut(s) 1317, 1581, 1606
BmrFI CCNGG 2 cut(s) 937, 1281
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 6 cut(s) 159, 319, 364, 1223, 1349, 1523
BmuI ACTGGG 1 cut(s) 420
BpiI GAAGAC 4 cut(s) 664, 744, 1157, 1258
BpmI CTGGAG 1 cut(s) 1307
BsaBI GATNNNNATC 1 cut(s) 1584
BsaI GGTCTC 1 cut(s) 748
BsaJI CCNNGG 4 cut(s) 319, 936, 1575, 1608
BsaWI WCCGGW 1 cut(s) 1034
BsaXI ACNNNNNCTCC 4 cut(s) 855, 885, 1596, 1626
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 631, 1310
Bse118I RCCGGY 1 cut(s) 1034
Bse1I ACTGG 7 cut(s) 22, 415, 434, 480, 1234, 1324, 1627
Bse3DI GCAATG 2 cut(s) 1696, 1713
Bse8I GATNNNNATC 1 cut(s) 1584
BseBI CCWGG 2 cut(s) 937, 1281
BseDI CCNNGG 4 cut(s) 319, 936, 1575, 1608
BseGI GGATG 3 cut(s) 1217, 1293, 1534
BseJI GATNNNNATC 1 cut(s) 1584
BseLI CCNNNNNNNGG 3 cut(s) 146, 631, 1310
BseMI GCAATG 2 cut(s) 1696, 1713
BseMII CTCAG 2 cut(s) 873, 1662
BseNI ACTGG 7 cut(s) 22, 415, 434, 480, 1234, 1324, 1627
BseRI GAGGAG 2 cut(s) 969, 1722
BseSI GKGCMC 1 cut(s) 1318
BseXI GCAGC 8 cut(s) 273, 808, 821, 865, 1066, 1318, 1416, 1718
BseYI CCCAGC 2 cut(s) 723, 881
Bsh1236I CGCG 1 cut(s) 1162
BshFI GGCC 1 cut(s) 1303
BshNI GGYRCC 1 cut(s) 1315
BshTI ACCGGT 1 cut(s) 1034
BsiSI CCGG 1 cut(s) 1035
BslFI GGGAC 3 cut(s) 427, 1493, 1667
BslI CCNNNNNNNGG 3 cut(s) 146, 631, 1310
BsmAI GTCTC 3 cut(s) 748, 960, 1563
BsmFI GGGAC 3 cut(s) 427, 1493, 1667
BsnI GGCC 1 cut(s) 1303
Bso31I GGTCTC 1 cut(s) 748
Bsp1286I GDGCHC 1 cut(s) 1318
Bsp143I GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
Bsp19I CCATGG 2 cut(s) 319, 1608
BspACI CCGC 1 cut(s) 504
BspANI GGCC 1 cut(s) 1303
BspCNI CTCAG 2 cut(s) 872, 1663
BspFNI CGCG 1 cut(s) 1162
BspHI TCATGA 2 cut(s) 1177, 1723
BspLI GGNNCC 3 cut(s) 1317, 1581, 1606
BspMAI CTGCAG 1 cut(s) 1084
BspPI GGATC 4 cut(s) 1574, 1587, 1721, 1763
BspQI GCTCTTC 1 cut(s) 1739
BspT107I GGYRCC 1 cut(s) 1315
BspTNI GGTCTC 1 cut(s) 748
BsrDI GCAATG 2 cut(s) 1696, 1713
BsrFI RCCGGY 1 cut(s) 1034
BsrI ACTGG 7 cut(s) 22, 415, 434, 480, 1234, 1324, 1627
BssAI RCCGGY 1 cut(s) 1034
BssECI CCNNGG 4 cut(s) 319, 936, 1575, 1608
BssMI GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
BssSI CACGAG 1 cut(s) 1543
BssT1I CCWWGG 3 cut(s) 319, 1575, 1608
Bst2BI CACGAG 1 cut(s) 1543
Bst2UI CCWGG 2 cut(s) 937, 1281
Bst4CI ACNGT 6 cut(s) 67, 294, 580, 692, 851, 1423
Bst6I CTCTTC 2 cut(s) 1739, 1748
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 1 cut(s) 1235
BstDEI CTNAG 4 cut(s) 859, 1349, 1671, 1740
BstDSI CCRYGG 2 cut(s) 319, 1608
BstEII GGTNACC 1 cut(s) 17
BstF5I GGATG 3 cut(s) 1217, 1293, 1534
BstFNI CGCG 1 cut(s) 1162
BstKTI GATC 6 cut(s) 225, 1183, 1378, 1582, 1729, 1771
BstMAI GTCTC 3 cut(s) 748, 960, 1563
BstMBI GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
BstMWI GCNNNNNNNGC 5 cut(s) 307, 818, 1337, 1404, 1715
BstNI CCWGG 2 cut(s) 937, 1281
BstPI GGTNACC 1 cut(s) 17
BstSCI CCNGG 2 cut(s) 935, 1279
BstSFI CTRYAG 3 cut(s) 114, 546, 1080
BstSLI GKGCMC 1 cut(s) 1318
BstUI CGCG 1 cut(s) 1162
BstV1I GCAGC 8 cut(s) 273, 808, 821, 865, 1066, 1318, 1416, 1718
BstV2I GAAGAC 4 cut(s) 664, 744, 1157, 1258
BstX2I RGATCY 2 cut(s) 222, 1579
BstXI CCANNNNNNTGG 1 cut(s) 1780
BstYI RGATCY 2 cut(s) 222, 1579
BsuRI GGCC 1 cut(s) 1303
BtgI CCRYGG 2 cut(s) 319, 1608
BtgZI GCGATG 1 cut(s) 136
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 3 cut(s) 1217, 1293, 1534
BtsI GCAGTG 3 cut(s) 164, 658, 1405
BtsIMutI CAGTG 8 cut(s) 164, 408, 619, 658, 697, 856, 1405, 1668
Cac8I GCNNGC 1 cut(s) 1235
CciI TCATGA 2 cut(s) 1177, 1723
Cfr10I RCCGGY 1 cut(s) 1034
Cfr13I GGNCC 2 cut(s) 323, 1619
CseI GACGC 1 cut(s) 1151
CsiI ACCWGGT 1 cut(s) 1279
Csp6I GTAC 2 cut(s) 1360, 1499
CspAI ACCGGT 1 cut(s) 1034
CspCI CAANNNNNGTGG 2 cut(s) 480, 515
CviQI GTAC 2 cut(s) 1360, 1499
DdeI CTNAG 4 cut(s) 859, 1349, 1671, 1740
DpnI GATC 6 cut(s) 224, 1182, 1377, 1581, 1728, 1770
DpnII GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
DraI TTTAAA 2 cut(s) 838, 1209
DraIII CACNNNGTG 1 cut(s) 1283
Eam1104I CTCTTC 2 cut(s) 1739, 1748
EarI CTCTTC 2 cut(s) 1739, 1748
Eco130I CCWWGG 3 cut(s) 319, 1575, 1608
Eco147I AGGCCT 1 cut(s) 1303
Eco31I GGTCTC 1 cut(s) 748
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 2 cut(s) 323, 1619
Eco57I CTGAAG 3 cut(s) 78, 756, 1252
Eco91I GGTNACC 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 935, 1279
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 3 cut(s) 319, 1575, 1608
ErhI CCWWGG 3 cut(s) 319, 1575, 1608
FaqI GGGAC 3 cut(s) 427, 1493, 1667
Fnu4HI GCNGC 8 cut(s) 287, 810, 822, 879, 1080, 1332, 1405, 1707
FokI GGATG 3 cut(s) 1204, 1280, 1541
Fsp4HI GCNGC 8 cut(s) 287, 810, 822, 879, 1080, 1332, 1405, 1707
FspBI CTAG 6 cut(s) 14, 174, 708, 963, 1436, 1677
GluI GCNGC 8 cut(s) 287, 810, 822, 879, 1080, 1332, 1405, 1707
GsaI CCCAGC 2 cut(s) 727, 885
GsuI CTGGAG 1 cut(s) 1307
HaeIII GGCC 1 cut(s) 1303
HapII CCGG 1 cut(s) 1035
HgaI GACGC 1 cut(s) 1151
HincII GTYRAC 2 cut(s) 27, 1535
HindII GTYRAC 2 cut(s) 27, 1535
HindIII AAGCTT 2 cut(s) 1133, 1200
HinfI GANTC 9 cut(s) 44, 161, 452, 515, 703, 959, 1263, 1571, 1813
HpaII CCGG 1 cut(s) 1035
HphI GGTGA 3 cut(s) 308, 390, 1049
Hpy166II GTNNAC 5 cut(s) 27, 856, 1501, 1535, 1668
Hpy188I TCNGA 6 cut(s) 73, 339, 753, 1105, 1491, 1590
Hpy188III TCNNGA 6 cut(s) 456, 519, 861, 963, 1178, 1724
Hpy8I GTNNAC 5 cut(s) 27, 856, 1501, 1535, 1668
Hpy99I CGWCG 3 cut(s) 84, 848, 1263
HpyAV CCTTC 4 cut(s) 102, 180, 336, 376
HpyCH4III ACNGT 6 cut(s) 67, 294, 580, 692, 851, 1423
HpyCH4IV ACGT 2 cut(s) 79, 232
HpyF10VI GCNNNNNNNGC 5 cut(s) 307, 818, 1337, 1404, 1715
HpyF3I CTNAG 4 cut(s) 859, 1349, 1671, 1740
HpySE526I ACGT 2 cut(s) 79, 232
Kzo9I GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
LguI GCTCTTC 1 cut(s) 1739
LmnI GCTCC 2 cut(s) 886, 1604
Lsp1109I GCAGC 8 cut(s) 273, 808, 821, 865, 1066, 1318, 1416, 1718
LweI GCATC 6 cut(s) 159, 319, 364, 1223, 1349, 1523
MabI ACCWGGT 1 cut(s) 1279
MaeI CTAG 6 cut(s) 14, 174, 708, 963, 1436, 1677
MaeII ACGT 2 cut(s) 79, 232
MaeIII GTNAC 4 cut(s) 17, 176, 482, 1037
MalI GATC 6 cut(s) 224, 1182, 1377, 1581, 1728, 1770
MboI GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
MboII GAAGA 9 cut(s) 217, 361, 664, 749, 1157, 1258, 1370, 1756, 1765
MflI RGATCY 2 cut(s) 222, 1579
MhlI GDGCHC 1 cut(s) 1318
MluI ACGCGT 1 cut(s) 1160
MlyI GAGTC 3 cut(s) 697, 1272, 1565
MmeI TCCRAC 2 cut(s) 554, 1245
MseI TTAA 4 cut(s) 569, 837, 1208, 1682
MslI CAYNNNNRTG 2 cut(s) 318, 1509
MspI CCGG 1 cut(s) 1035
MspR9I CCNGG 2 cut(s) 937, 1281
MvaI CCWGG 2 cut(s) 937, 1281
MvnI CGCG 1 cut(s) 1162
MwoI GCNNNNNNNGC 5 cut(s) 307, 818, 1337, 1404, 1715
NcoI CCATGG 2 cut(s) 319, 1608
NdeII GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
NlaIV GGNNCC 3 cut(s) 1317, 1581, 1606
NmuCI GTSAC 1 cut(s) 1037
PagI TCATGA 2 cut(s) 1177, 1723
PceI AGGCCT 1 cut(s) 1303
PciSI GCTCTTC 1 cut(s) 1739
PfeI GAWTC 6 cut(s) 44, 161, 452, 515, 959, 1813
PflFI GACNNNGTC 1 cut(s) 626
PinAI ACCGGT 1 cut(s) 1034
PkrI GCNGC 8 cut(s) 288, 811, 823, 880, 1081, 1333, 1406, 1708
PleI GAGTC 3 cut(s) 697, 1271, 1565
PpsI GAGTC 3 cut(s) 697, 1271, 1565
PsiI TTATAA 2 cut(s) 975, 990
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 935, 1279
PspEI GGTNACC 1 cut(s) 17
PspFI CCCAGC 2 cut(s) 723, 881
PspGI CCWGG 2 cut(s) 935, 1279
PspN4I GGNNCC 3 cut(s) 1317, 1581, 1606
PspPI GGNCC 2 cut(s) 323, 1619
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 2 cut(s) 222, 1579
PsyI GACNNNGTC 1 cut(s) 626
RsaI GTAC 2 cut(s) 1361, 1500
RsaNI GTAC 2 cut(s) 1360, 1499
RseI CAYNNNNRTG 2 cut(s) 318, 1509
SapI GCTCTTC 1 cut(s) 1739
SaqAI TTAA 4 cut(s) 569, 837, 1208, 1682
SatI GCNGC 8 cut(s) 287, 810, 822, 879, 1080, 1332, 1405, 1707
Sau3AI GATC 6 cut(s) 222, 1180, 1375, 1579, 1726, 1768
Sau96I GGNCC 2 cut(s) 323, 1619
SchI GAGTC 3 cut(s) 697, 1272, 1565
ScrFI CCNGG 2 cut(s) 937, 1281
SduI GDGCHC 1 cut(s) 1318
SexAI ACCWGGT 1 cut(s) 1279
SfaNI GCATC 6 cut(s) 159, 319, 364, 1223, 1349, 1523
SfcI CTRYAG 3 cut(s) 114, 546, 1080
SinI GGWCC 2 cut(s) 323, 1619
SmiMI CAYNNNNRTG 2 cut(s) 318, 1509
SseBI AGGCCT 1 cut(s) 1303
SsiI CCGC 1 cut(s) 504
SspMI CTAG 6 cut(s) 14, 174, 708, 963, 1436, 1677
StuI AGGCCT 1 cut(s) 1303
StyD4I CCNGG 2 cut(s) 935, 1279
StyI CCWWGG 3 cut(s) 319, 1575, 1608
TaaI ACNGT 6 cut(s) 67, 294, 580, 692, 851, 1423
TaiI ACGT 2 cut(s) 82, 235
TaqI TCGA 4 cut(s) 372, 445, 1119, 1261
TatI WGTACW 1 cut(s) 1498
TfiI GAWTC 6 cut(s) 44, 161, 452, 515, 959, 1813
Tru1I TTAA 4 cut(s) 569, 837, 1208, 1682
Tru9I TTAA 4 cut(s) 569, 837, 1208, 1682
TscAI CASTG 8 cut(s) 171, 415, 619, 658, 697, 856, 1405, 1675
TseFI GTSAC 1 cut(s) 1037
TseI GCWGC 8 cut(s) 286, 809, 821, 878, 1079, 1331, 1404, 1706
Tsp45I GTSAC 1 cut(s) 1037
TspDTI ATGAA 9 cut(s) 545, 664, 816, 1035, 1107, 1166, 1212, 1466, 1712
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 8 cut(s) 171, 415, 619, 658, 697, 856, 1405, 1675
Tth111I GACNNNGTC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 323, 1619
XapI RAATTY 4 cut(s) 440, 472, 1415, 1637
XbaI TCTAGA 1 cut(s) 962
XspI CTAG 6 cut(s) 14, 174, 708, 963, 1436, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.