Rh7DG078100

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011561
Physical Location & Seq
Forward (+)
6212555 .. 6216812
4258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG078100.1

Sequence Viewer

Length: 1872 bp
ATGCCATCTTCACTAGGTTACCAGTCAACTGCAACAGACCAAGAATCCGTGATGTTTGTCCATACAGTTTCAGAGGCACGTCGTATGCTTCCTTCAGCAAGTCGCTGGAACTCTATAGAAATTGACTTCAATCTTGTTCCTCAATCTGGCATCGCCTATGATTCACTGCCTTCTAGTTACTCCAAGTCGTATGATTACGATATTGTTATTAGAGATAGAAAGATCTTCAAACGTTGTCTTTACATTTCTGCCTCAATAGTTAGTCTTATTGTAGCTCTAATTTTGCTGCTACGGTTTTTGCCTCACAAGCATCATCACCATGGTCCTTCAAAAAATCTGACACTTGCATTGAACCAAGCTCTAACCTTCTTCGATGCTCAAAAATCTGGGACTTACCCCAAAAATAGTCCAGTAAAATTTCGAGGGGATTCAGGATTGCAAGATGGGAATTTGACCAGTAACCACGCAAATCTTGTGGGCGGTTTCTATGATTCTGGAAACAACATGAAGTTCAGTTTCTCTACAGCTTATACCATGACCTTGTTAAGTTGGACTGTGATTGAATACCACCAAAAATATGCTGATATCAGTGAGCTTGACCATGTCAAGGATATAATCAAATGGGGCAGTGATTATTTGCTCAAAGTCTTCGTTCCTCCAAATGCGACTTCAGATACCACATTGTATTCTCAGATTGGAAGTGCAAACAGTGATGGCACGACTCCTAGTGATATAAACTGCTGGGAAAGACCTGAAGACATGGATTATCAGAGACCTGTTTCAATTTGTGATAGCACAGCTACCGATTTAGCAGGGGAAATTGTTGCAGCATTATCTGCTGCTTCATTAGTATTTAAAGACGACGACAGTGTTTACTCAGGAGAATTAGTGAAAGCTGCTGGGGAGCTATTTGAGAGTGCAACTAAAGACCCTAATAATCAAGGAACTTACACCAGGGTTGAAACTTGTGGAGGAGAATCTAGAGACTTTTATAACTCATCTGGTTATAAAGACGAATTAGTTTGGGGAGGAACTTGGTTATTTTTTGCTACCGGTGACATTTCATATCTGCAATATGCCACAGACAAGTTTCAGGCTGCAGTTGATGATGAAACAAGTTCTGATAAATGGATTTTCGATTGGAACAACAAGCTTACTGCTAACATGGTGTTGCTAACGCGTCTTCAATTCTTTCATGATCTTGGCTACCCGTATGAAGCTTCTTTAAAGGCATCATCCAACATGACTGGCTCGCTTATGTGTTCTTATGTTTCTCGTCGAGTCTTCAGCATGACACCAGGTGGGTTGGTCATCCAAAGGCCTGATTATGGGGCACCTCTCCAGTTTGCTGCAACAGCATCTTTCCTTAGTAAATTGTACGGCGACTACCTTGATCTTCTGCGTAGTTCTGGTGGCAGTGGCAGCACTTGTGAATTTACCGTAGAAATGCTGCGGAATTTCTCCTTGTCTCAGGTGAATTACATTCTAGGAGATAACCCAATGAATATGAGCTACATTGTGGGCTTCGGGGACAAGTTTCCGAGCCAAGTACACCATAGAAGTGCATCAATCCTTTGGGATGGTCAACATTACTCGTGTAAAGATGGATATAAATGGCGAGACTCCAAGGATCCAAATCCGAATGTGCTGTTGGGAGCCATGGTAGGAGGACCAGACCAGTTTGACAAATTTATAGATGAGAGGGACAAGCAAGAGTTCACTGAGCCTAGATTAACAAGCAATGCTGGTTTAGTTGCAGCTCTCATTGCACTTCATGATCCTCCTCATACTAAGAAGAGCAAGAAGAGAGGCAACTATGATCCCACTAAATTGGGCATAGACCAGATGGGTATTTTTGATAAGATTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

623

Amino Acids

69.45

Weight (kDa)

5.55

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 116 - 588 2.7e-125 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 993, 1008
AccB1I GGYRCC 1 cut(s) 1333
AccII CGCG 1 cut(s) 1180
AciI CCGC 2 cut(s) 480, 1453
AclI AACGTT 1 cut(s) 232
AclWI GGATC 4 cut(s) 1625, 1638, 1772, 1814
AcsI RAATTY 5 cut(s) 416, 448, 1433, 1456, 1688
AcuI CTGAAG 4 cut(s) 78, 654, 774, 1270
AdeI CACNNNGTG 1 cut(s) 1301
AfaI GTAC 2 cut(s) 1379, 1551
AfiI CCNNNNNNNGG 3 cut(s) 146, 607, 1328
AflIII ACRYGT 1 cut(s) 1178
AgeI ACCGGT 1 cut(s) 1052
AgsI TTSAA 8 cut(s) 130, 229, 330, 352, 563, 783, 962, 1187
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 2 cut(s) 953, 1297
AjuI GAANNNNNNNTTGG 4 cut(s) 652, 684, 1634, 1666
Alw26I GTCTC 4 cut(s) 766, 978, 1473, 1614
AlwI GGATC 4 cut(s) 1625, 1638, 1772, 1814
AoxI GGCC 1 cut(s) 1319
ApeKI GCWGC 9 cut(s) 286, 827, 839, 896, 1097, 1349, 1422, 1450, 1757
ApoI RAATTY 5 cut(s) 416, 448, 1433, 1456, 1688
AsiGI ACCGGT 1 cut(s) 1052
AspS9I GGNCC 2 cut(s) 323, 1670
AsuHPI GGTGA 3 cut(s) 308, 1067, 1486
AvaII GGWCC 2 cut(s) 323, 1670
BaeGI GKGCMC 1 cut(s) 1336
BamHI GGATCC 1 cut(s) 1630
BanI GGYRCC 1 cut(s) 1333
BarI GAAGNNNNNNTAC 2 cut(s) 76, 108
BauI CACGAG 1 cut(s) 1594
BbsI GAAGAC 4 cut(s) 640, 762, 1175, 1276
BbvI GCAGC 9 cut(s) 273, 826, 839, 883, 1084, 1336, 1434, 1437, 1769
BccI CCATC 6 cut(s) 13, 437, 707, 1574, 1598, 1840
BceAI ACGGC 1 cut(s) 1396
BciT130I CCWGG 2 cut(s) 955, 1299
BcoDI GTCTC 4 cut(s) 766, 978, 1473, 1614
BfaI CTAG 6 cut(s) 14, 174, 726, 981, 1487, 1728
BfmI CTRYAG 3 cut(s) 114, 522, 1098
BglII AGATCT 1 cut(s) 222
BisI GCNGC 9 cut(s) 287, 828, 840, 897, 1098, 1350, 1423, 1451, 1758
BlsI GCNGC 9 cut(s) 288, 829, 841, 898, 1099, 1351, 1424, 1452, 1759
Bme1390I CCNGG 2 cut(s) 955, 1299
Bme18I GGWCC 2 cut(s) 323, 1670
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 323, 1670
BmiI GGNNCC 3 cut(s) 1335, 1632, 1657
BmrFI CCNGG 2 cut(s) 955, 1299
BmsI GCATC 6 cut(s) 159, 319, 364, 1241, 1367, 1574
BpiI GAAGAC 4 cut(s) 640, 762, 1175, 1276
BpmI CTGGAG 1 cut(s) 1325
BsaBI GATNNNNATC 1 cut(s) 1635
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 4 cut(s) 319, 954, 1626, 1659
BsaWI WCCGGW 1 cut(s) 1052
BsaXI ACNNNNNCTCC 4 cut(s) 873, 903, 1647, 1677
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 607, 1328
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 6 cut(s) 22, 410, 456, 1252, 1342, 1678
Bse3DI GCAATG 2 cut(s) 1747, 1764
Bse8I GATNNNNATC 1 cut(s) 1635
BseBI CCWGG 2 cut(s) 955, 1299
BseDI CCNNGG 4 cut(s) 319, 954, 1626, 1659
BseGI GGATG 3 cut(s) 1235, 1311, 1585
BseJI GATNNNNATC 1 cut(s) 1635
BseLI CCNNNNNNNGG 3 cut(s) 146, 607, 1328
BseMI GCAATG 2 cut(s) 1747, 1764
BseMII CTCAG 4 cut(s) 704, 891, 1484, 1713
BseNI ACTGG 6 cut(s) 22, 410, 456, 1252, 1342, 1678
BseRI GAGGAG 2 cut(s) 987, 1773
BseSI GKGCMC 1 cut(s) 1336
BseXI GCAGC 9 cut(s) 273, 826, 839, 883, 1084, 1336, 1434, 1437, 1769
BseYI CCCAGC 2 cut(s) 741, 899
Bsh1236I CGCG 1 cut(s) 1180
BshFI GGCC 1 cut(s) 1321
BshNI GGYRCC 1 cut(s) 1333
BshTI ACCGGT 1 cut(s) 1052
BsiSI CCGG 1 cut(s) 1053
BslFI GGGAC 3 cut(s) 403, 1544, 1718
BslI CCNNNNNNNGG 3 cut(s) 146, 607, 1328
BsmAI GTCTC 4 cut(s) 766, 978, 1473, 1614
BsmFI GGGAC 3 cut(s) 403, 1544, 1718
BsnI GGCC 1 cut(s) 1321
Bso31I GGTCTC 1 cut(s) 766
Bsp1286I GDGCHC 1 cut(s) 1336
Bsp143I GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
Bsp19I CCATGG 2 cut(s) 319, 1659
BspACI CCGC 2 cut(s) 480, 1453
BspANI GGCC 1 cut(s) 1321
BspCNI CTCAG 4 cut(s) 703, 890, 1483, 1714
BspFNI CGCG 1 cut(s) 1180
BspHI TCATGA 2 cut(s) 1195, 1774
BspLI GGNNCC 3 cut(s) 1335, 1632, 1657
BspMAI CTGCAG 1 cut(s) 1102
BspPI GGATC 4 cut(s) 1625, 1638, 1772, 1814
BspQI GCTCTTC 1 cut(s) 1790
BspT107I GGYRCC 1 cut(s) 1333
BspTNI GGTCTC 1 cut(s) 766
BsrDI GCAATG 2 cut(s) 1747, 1764
BsrFI RCCGGY 1 cut(s) 1052
BsrI ACTGG 6 cut(s) 22, 410, 456, 1252, 1342, 1678
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 4 cut(s) 319, 954, 1626, 1659
BssMI GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
BssSI CACGAG 1 cut(s) 1594
BssT1I CCWWGG 3 cut(s) 319, 1626, 1659
Bst2BI CACGAG 1 cut(s) 1594
Bst2UI CCWGG 2 cut(s) 955, 1299
Bst4CI ACNGT 6 cut(s) 67, 294, 556, 710, 869, 1441
Bst6I CTCTTC 2 cut(s) 1790, 1799
BstAPI GCANNNNNTGC 1 cut(s) 836
BstC8I GCNNGC 1 cut(s) 1253
BstDEI CTNAG 6 cut(s) 690, 877, 1367, 1470, 1722, 1791
BstDSI CCRYGG 2 cut(s) 319, 1659
BstEII GGTNACC 1 cut(s) 17
BstF5I GGATG 3 cut(s) 1235, 1311, 1585
BstFNI CGCG 1 cut(s) 1180
BstKTI GATC 6 cut(s) 225, 1201, 1396, 1633, 1780, 1822
BstMAI GTCTC 4 cut(s) 766, 978, 1473, 1614
BstMBI GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
BstMWI GCNNNNNNNGC 5 cut(s) 307, 836, 1355, 1422, 1766
BstNI CCWGG 2 cut(s) 955, 1299
BstPI GGTNACC 1 cut(s) 17
BstSCI CCNGG 2 cut(s) 953, 1297
BstSFI CTRYAG 3 cut(s) 114, 522, 1098
BstSLI GKGCMC 1 cut(s) 1336
BstUI CGCG 1 cut(s) 1180
BstV1I GCAGC 9 cut(s) 273, 826, 839, 883, 1084, 1336, 1434, 1437, 1769
BstV2I GAAGAC 4 cut(s) 640, 762, 1175, 1276
BstX2I RGATCY 2 cut(s) 222, 1630
BstXI CCANNNNNNTGG 1 cut(s) 1831
BstYI RGATCY 2 cut(s) 222, 1630
BsuRI GGCC 1 cut(s) 1321
BtgI CCRYGG 2 cut(s) 319, 1659
BtgZI GCGATG 1 cut(s) 136
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 3 cut(s) 1235, 1311, 1585
BtsI GCAGTG 3 cut(s) 164, 634, 1423
BtsIMutI CAGTG 7 cut(s) 164, 595, 634, 715, 874, 1423, 1719
Cac8I GCNNGC 1 cut(s) 1253
CciI TCATGA 2 cut(s) 1195, 1774
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 2 cut(s) 323, 1670
CseI GACGC 1 cut(s) 1169
CsiI ACCWGGT 1 cut(s) 1297
Csp6I GTAC 2 cut(s) 1378, 1550
CspAI ACCGGT 1 cut(s) 1052
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviQI GTAC 2 cut(s) 1378, 1550
DdeI CTNAG 6 cut(s) 690, 877, 1367, 1470, 1722, 1791
DpnI GATC 6 cut(s) 224, 1200, 1395, 1632, 1779, 1821
DpnII GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
DraI TTTAAA 2 cut(s) 856, 1227
DraIII CACNNNGTG 1 cut(s) 1301
Eam1104I CTCTTC 2 cut(s) 1790, 1799
EarI CTCTTC 2 cut(s) 1790, 1799
Eco130I CCWWGG 3 cut(s) 319, 1626, 1659
Eco147I AGGCCT 1 cut(s) 1321
Eco31I GGTCTC 1 cut(s) 766
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 323, 1670
Eco57I CTGAAG 4 cut(s) 78, 654, 774, 1270
Eco91I GGTNACC 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 953, 1297
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 3 cut(s) 319, 1626, 1659
ErhI CCWWGG 3 cut(s) 319, 1626, 1659
FaqI GGGAC 3 cut(s) 403, 1544, 1718
Fnu4HI GCNGC 9 cut(s) 287, 828, 840, 897, 1098, 1350, 1423, 1451, 1758
FokI GGATG 3 cut(s) 1222, 1298, 1592
Fsp4HI GCNGC 9 cut(s) 287, 828, 840, 897, 1098, 1350, 1423, 1451, 1758
FspBI CTAG 6 cut(s) 14, 174, 726, 981, 1487, 1728
GluI GCNGC 9 cut(s) 287, 828, 840, 897, 1098, 1350, 1423, 1451, 1758
GsaI CCCAGC 2 cut(s) 745, 903
GsuI CTGGAG 1 cut(s) 1325
HaeIII GGCC 1 cut(s) 1321
HapII CCGG 1 cut(s) 1053
HgaI GACGC 1 cut(s) 1169
HincII GTYRAC 2 cut(s) 27, 1586
HindII GTYRAC 2 cut(s) 27, 1586
HindIII AAGCTT 2 cut(s) 1151, 1218
HinfI GANTC 9 cut(s) 44, 161, 428, 491, 721, 977, 1281, 1622, 1864
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 3 cut(s) 308, 1067, 1486
Hpy166II GTNNAC 5 cut(s) 27, 874, 1552, 1586, 1719
Hpy188I TCNGA 8 cut(s) 73, 339, 673, 693, 771, 1123, 1542, 1641
Hpy188III TCNNGA 6 cut(s) 432, 495, 879, 981, 1196, 1775
Hpy8I GTNNAC 5 cut(s) 27, 874, 1552, 1586, 1719
Hpy99I CGWCG 3 cut(s) 84, 866, 1281
HpyAV CCTTC 4 cut(s) 102, 180, 336, 376
HpyCH4III ACNGT 6 cut(s) 67, 294, 556, 710, 869, 1441
HpyCH4IV ACGT 2 cut(s) 79, 232
HpyF10VI GCNNNNNNNGC 5 cut(s) 307, 836, 1355, 1422, 1766
HpyF3I CTNAG 6 cut(s) 690, 877, 1367, 1470, 1722, 1791
HpySE526I ACGT 2 cut(s) 79, 232
Kzo9I GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
LguI GCTCTTC 1 cut(s) 1790
LmnI GCTCC 2 cut(s) 904, 1655
Lsp1109I GCAGC 9 cut(s) 273, 826, 839, 883, 1084, 1336, 1434, 1437, 1769
LweI GCATC 6 cut(s) 159, 319, 364, 1241, 1367, 1574
MabI ACCWGGT 1 cut(s) 1297
MaeI CTAG 6 cut(s) 14, 174, 726, 981, 1487, 1728
MaeII ACGT 2 cut(s) 79, 232
MaeIII GTNAC 4 cut(s) 17, 176, 458, 1055
MalI GATC 6 cut(s) 224, 1200, 1395, 1632, 1779, 1821
MboI GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
MboII GAAGA 9 cut(s) 217, 361, 640, 767, 1175, 1276, 1388, 1807, 1816
MflI RGATCY 2 cut(s) 222, 1630
MhlI GDGCHC 1 cut(s) 1336
MluI ACGCGT 1 cut(s) 1178
MlyI GAGTC 3 cut(s) 715, 1290, 1616
MmeI TCCRAC 2 cut(s) 530, 1263
MseI TTAA 4 cut(s) 545, 855, 1226, 1733
MslI CAYNNNNRTG 2 cut(s) 318, 1560
MspI CCGG 1 cut(s) 1053
MspR9I CCNGG 2 cut(s) 955, 1299
MvaI CCWGG 2 cut(s) 955, 1299
MvnI CGCG 1 cut(s) 1180
MwoI GCNNNNNNNGC 5 cut(s) 307, 836, 1355, 1422, 1766
NcoI CCATGG 2 cut(s) 319, 1659
NdeII GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
NlaIV GGNNCC 3 cut(s) 1335, 1632, 1657
NmuCI GTSAC 1 cut(s) 1055
PagI TCATGA 2 cut(s) 1195, 1774
PceI AGGCCT 1 cut(s) 1321
PciSI GCTCTTC 1 cut(s) 1790
PfeI GAWTC 6 cut(s) 44, 161, 428, 491, 977, 1864
PflFI GACNNNGTC 1 cut(s) 602
PinAI ACCGGT 1 cut(s) 1052
PkrI GCNGC 9 cut(s) 288, 829, 841, 898, 1099, 1351, 1424, 1452, 1759
PleI GAGTC 3 cut(s) 715, 1289, 1616
PpsI GAGTC 3 cut(s) 715, 1289, 1616
PsiI TTATAA 2 cut(s) 993, 1008
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 953, 1297
PspEI GGTNACC 1 cut(s) 17
PspFI CCCAGC 2 cut(s) 741, 899
PspGI CCWGG 2 cut(s) 953, 1297
PspN4I GGNNCC 3 cut(s) 1335, 1632, 1657
PspPI GGNCC 2 cut(s) 323, 1670
PstI CTGCAG 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 222, 1630
PsyI GACNNNGTC 1 cut(s) 602
RsaI GTAC 2 cut(s) 1379, 1551
RsaNI GTAC 2 cut(s) 1378, 1550
RseI CAYNNNNRTG 2 cut(s) 318, 1560
SapI GCTCTTC 1 cut(s) 1790
SaqAI TTAA 4 cut(s) 545, 855, 1226, 1733
SatI GCNGC 9 cut(s) 287, 828, 840, 897, 1098, 1350, 1423, 1451, 1758
Sau3AI GATC 6 cut(s) 222, 1198, 1393, 1630, 1777, 1819
Sau96I GGNCC 2 cut(s) 323, 1670
SchI GAGTC 3 cut(s) 715, 1290, 1616
ScrFI CCNGG 2 cut(s) 955, 1299
SduI GDGCHC 1 cut(s) 1336
SexAI ACCWGGT 1 cut(s) 1297
SfaNI GCATC 6 cut(s) 159, 319, 364, 1241, 1367, 1574
SfcI CTRYAG 3 cut(s) 114, 522, 1098
SinI GGWCC 2 cut(s) 323, 1670
SmiMI CAYNNNNRTG 2 cut(s) 318, 1560
SseBI AGGCCT 1 cut(s) 1321
SsiI CCGC 2 cut(s) 480, 1453
SspMI CTAG 6 cut(s) 14, 174, 726, 981, 1487, 1728
StuI AGGCCT 1 cut(s) 1321
StyD4I CCNGG 2 cut(s) 953, 1297
StyI CCWWGG 3 cut(s) 319, 1626, 1659
TaaI ACNGT 6 cut(s) 67, 294, 556, 710, 869, 1441
TaiI ACGT 2 cut(s) 82, 235
TaqI TCGA 4 cut(s) 372, 421, 1137, 1279
TatI WGTACW 1 cut(s) 1549
TfiI GAWTC 6 cut(s) 44, 161, 428, 491, 977, 1864
Tru1I TTAA 4 cut(s) 545, 855, 1226, 1733
Tru9I TTAA 4 cut(s) 545, 855, 1226, 1733
TscAI CASTG 7 cut(s) 171, 595, 634, 715, 874, 1423, 1726
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 9 cut(s) 286, 827, 839, 896, 1097, 1349, 1422, 1450, 1757
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 8 cut(s) 521, 834, 1053, 1125, 1184, 1230, 1517, 1763
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 7 cut(s) 171, 595, 634, 715, 874, 1423, 1726
Tth111I GACNNNGTC 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 323, 1670
XapI RAATTY 5 cut(s) 416, 448, 1433, 1456, 1688
XbaI TCTAGA 1 cut(s) 980
XspI CTAG 6 cut(s) 14, 174, 726, 981, 1487, 1728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.