MD14G1229100.v1.1

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011561
Physical Location & Seq
Forward (+)
31045240 .. 31047826
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1229100.v1.1.491

Sequence Viewer

Length: 1911 bp
ATGACATCTTCTCTAGGTTCCCAGACACAAGCATCGGCAGCAGACCTAGAACCTGTGAGATTTGTCCATACAGTTTCCGAGGCCCGCCGTTTGCTTCCCTCAGCGAGTCGTTGGAACTCCATAGAAATTGACTATAACCTAGTACCTCAGTCTTCCATAGCCAATGATTCAATCCCTTCTCCGTACTCCAAGTCTTACGAATTCAATCTCGTTATCACCGATAAAAACTACCTCAAACGTTGTCTTTATATATCTGCATCCACTATTTTTGTTGTCCTAGCTCTAATTTTACTGGTAGCACTTTTGCCTAATGCTCTTCAAAAGCATCATCGCCACCCTTCTAAGAATCTTACGCGTGCATTGAACCAAGCTCTGACGTTCTTTGACGCGCAAATATCTGGGGTTTACCCTAATAATAGTCCGGTAAAATTTCGCGCCAGTTCAGGATTGCATGATGGGAATTTGAGCAGTAAACCGGTGAATCTTCGGGGTGGTTTCTATGATTCTGGCAACAACATAAAGTTCAGCTTCCCTACAGCTTATACCGTAACCCTGTTGAGTTGGACTGTGATCGAATACCACGAAAAGTATGCTGATATTGGTGAGCTTGATCATGTCAAGGACATAATAAAATGGGGCAGTGATTATTTGCTCAAACTTTTTGTTCCTCCAAACACAACTTCAGAGTTCATATTGTATTCTCAGGCAAGCCTTCTGAAAAATTTAAGTCCCGTTGGAAGTGGAAACATTGATGCCAAGGTTCCTAATGACATAAACTGCTGGCAAAGGCCTGAAGACATGCATTACGACAGACCTGTTTCGATCTGCGACAGCACTGCTTCTGATTTAGCAGGGGAAATTATTGCAGCATTGTCCGCCGCTTCATTAGTGTTTAAAGAAGACAATGCATACTCAGGAGAACTAGTTACTGCAGCTGAGAAGCTATACGAGAGTGCAGTTACTAAACTAGAGCCTGCTAGGCAAGGAGCATACACCAAGGTTGATGCTTGTGGAGTAGAATCTAGGAATTTCTACAACTCTTCTGGTTTCCAAGATGAACTGGTCTGGGGAGGAACATGGTTATTTTTCGCTACCGGCGATAATTCATATCTAGATTATGCCACAAAAGAATTGAAGTATGCAGTTGCTAACGAAACGAGTGCTGATAAAGGGATTTTCTACTGGAACAACAAGCTCACTGCCAATGCGGTCTTGCTAACACGTCTCCGATATTTTCGTGATCTTGGATTCCCGTATGAATCTTCTTTGGACGCATCATCAGACATTACTGACACCCTCATGTGTTCTTATTTTTCTTCTCAGAAAACAACACCAGGTGGATTGCTCCTCCAAAGGCCTACTTATGGCGCGCGGGCACCTCTCGAATATGCTGCAACAGCATCTTTTCTTAGTAAATTGTACAGCGACTACCTTGACCTTCTGCGTAGTTCCGGTGGGAGTTGCATTAATTTTGGCTTTTCCATCGATATGTTGCGCAATTTCTCAATAACTCAGGCAAGTGTCAATTACATTCTAGGAGATAATCCAATGAAGATGAGCTACATGGTTGGCTTCGGAGATAAGTTTCCAACCCAAGTGCACCACAGAAGCGCATCGATCCCTTGGGACGGTCATCAGTACTCTTGTGAAGATGGAGAGAAATGGAAAAACTCCAAGGACCCAAATCCGAATTTGCTTTTGGGAGCCATGGTAGCAGGGCCCGACGAATTGGACAAGTTCGTAGATGAAAGGGACAAGCAGGAGTTCACTGAGCCTAGCATAGCAAGCAATGCTGGTTTAGTTGCGGCACTCATTGCACTTCATGATCCTCCTCGCCATCGTTCTTCGAAGGCCAATGGCACGCATTTGGGGATAGACCAGACGGGTATTTTTGGTAAGATCCGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

637

Amino Acids

70.37

Weight (kDa)

5.87

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 119 - 604 3.7e-117 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1496
AccB1I GGYRCC 1 cut(s) 1375
AccII CGCG 5 cut(s) 355, 389, 435, 1370, 1372
AciI CCGC 6 cut(s) 85, 876, 879, 1208, 1372, 1805
AclI AACGTT 1 cut(s) 238
AclWI GGATC 3 cut(s) 1612, 1820, 1894
AcsI RAATTY 6 cut(s) 200, 428, 460, 721, 1027, 1690
AcuI CTGAAG 2 cut(s) 666, 813
AdeI CACNNNGTG 1 cut(s) 1337
AfaI GTAC 4 cut(s) 144, 185, 1421, 1640
AfiI CCNNNNNNNGG 2 cut(s) 1364, 1628
AflIII ACRYGT 2 cut(s) 353, 1220
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 5 cut(s) 171, 205, 320, 364, 1135
AhlI ACTAGT 1 cut(s) 922
AjiI CACGTC 1 cut(s) 1223
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 4 cut(s) 664, 696, 1682, 1714
AluBI AGCT 9 cut(s) 281, 371, 528, 539, 607, 935, 943, 1195, 1560
AluI AGCT 9 cut(s) 281, 371, 528, 539, 607, 935, 943, 1195, 1560
Alw21I GWGCWC 1 cut(s) 1602
Alw26I GTCTC 1 cut(s) 1229
Alw44I GTGCAC 1 cut(s) 1598
AlwI GGATC 3 cut(s) 1612, 1820, 1894
AoxI GGCC 5 cut(s) 81, 788, 1355, 1718, 1851
ApaI GGGCCC 1 cut(s) 1722
ApaLI GTGCAC 1 cut(s) 1598
ApeKI GCWGC 4 cut(s) 38, 866, 932, 1391
ApoI RAATTY 6 cut(s) 200, 428, 460, 721, 1027, 1690
AseI ATTAAT 1 cut(s) 1467
AsiGI ACCGGT 1 cut(s) 475
AspLEI GCGC 6 cut(s) 391, 437, 1370, 1372, 1497, 1613
AspS9I GGNCC 4 cut(s) 82, 1678, 1718, 1719
AsuHPI GGTGA 3 cut(s) 208, 490, 614
AsuII TTCGAA 1 cut(s) 1847
AvaII GGWCC 1 cut(s) 1678
BaeGI GKGCMC 3 cut(s) 1378, 1602, 1722
BanI GGYRCC 1 cut(s) 1375
BanII GRGCYC 1 cut(s) 1722
BarI GAAGNNNNNNTAC 2 cut(s) 1544, 1576
BbsI GAAGAC 3 cut(s) 144, 801, 906
Bbv12I GWGCWC 1 cut(s) 1602
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 50, 878, 944, 1378
BccI CCATC 4 cut(s) 449, 1490, 1646, 1845
BceAI ACGGC 1 cut(s) 72
BcgI CGANNNNNNTGC 8 cut(s) 572, 606, 818, 852, 1142, 1176, 1373, 1407
BciT130I CCWGG 1 cut(s) 1335
BclI TGATCA 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 1229
BcuI ACTAGT 1 cut(s) 922
BfmI CTRYAG 2 cut(s) 534, 930
BglI GCCNNNNNGGC 1 cut(s) 979
BisI GCNGC 6 cut(s) 39, 867, 879, 933, 1392, 1806
BlsI GCNGC 6 cut(s) 40, 868, 880, 934, 1393, 1807
BmcAI AGTACT 1 cut(s) 1640
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 1678
BmgBI CACGTC 1 cut(s) 1223
BmgT120I GGNCC 4 cut(s) 82, 1678, 1718, 1719
BmiI GGNNCC 6 cut(s) 19, 762, 1377, 1680, 1705, 1720
BmrFI CCNGG 1 cut(s) 1335
BmsI GCATC 8 cut(s) 41, 266, 334, 742, 994, 1283, 1409, 1622
BpiI GAAGAC 3 cut(s) 144, 801, 906
Bpu10I CCTNAGC 1 cut(s) 100
Bpu14I TTCGAA 1 cut(s) 1847
Bsa29I ATCGAT 2 cut(s) 1485, 1616
BsaJI CCNNGG 6 cut(s) 78, 756, 996, 1622, 1674, 1707
BsaWI WCCGGW 3 cut(s) 421, 475, 1451
BsaXI ACNNNNNCTCC 4 cut(s) 909, 939, 1695, 1725
Bsc4I CCNNNNNNNGG 2 cut(s) 1364, 1628
Bse118I RCCGGY 2 cut(s) 475, 1094
Bse1I ACTGG 4 cut(s) 297, 438, 1065, 1187
Bse3DI GCAATG 2 cut(s) 1795, 1812
BseBI CCWGG 1 cut(s) 1335
BseCI ATCGAT 2 cut(s) 1485, 1616
BseDI CCNNGG 6 cut(s) 78, 756, 996, 1622, 1674, 1707
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 1364, 1628
BseMI GCAATG 2 cut(s) 1795, 1812
BseMII CTCAG 8 cut(s) 114, 161, 716, 927, 927, 1334, 1526, 1761
BseNI ACTGG 4 cut(s) 297, 438, 1065, 1187
BsePI GCGCGC 1 cut(s) 1368
BseRI GAGGAG 2 cut(s) 1337, 1821
BseSI GKGCMC 3 cut(s) 1378, 1602, 1722
BseXI GCAGC 4 cut(s) 50, 878, 944, 1378
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 5 cut(s) 355, 389, 435, 1370, 1372
BshFI GGCC 5 cut(s) 83, 790, 1357, 1720, 1853
BshNI GGYRCC 1 cut(s) 1375
BshTI ACCGGT 1 cut(s) 475
BshVI ATCGAT 2 cut(s) 1485, 1616
BsiHKAI GWGCWC 1 cut(s) 1602
BsiSI CCGG 5 cut(s) 422, 476, 1095, 1452, 1903
BslFI GGGAC 3 cut(s) 714, 1640, 1766
BslI CCNNNNNNNGG 2 cut(s) 1364, 1628
BsmAI GTCTC 1 cut(s) 1229
BsmBI CGTCTC 1 cut(s) 1229
BsmFI GGGAC 3 cut(s) 714, 1640, 1766
BsnI GGCC 5 cut(s) 83, 790, 1357, 1720, 1853
Bsp119I TTCGAA 1 cut(s) 1847
Bsp120I GGGCCC 1 cut(s) 1718
Bsp1286I GDGCHC 3 cut(s) 1378, 1602, 1722
Bsp1407I TGTACA 1 cut(s) 1419
Bsp143I GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
Bsp19I CCATGG 1 cut(s) 1707
BspACI CCGC 6 cut(s) 85, 876, 879, 1208, 1372, 1805
BspANI GGCC 5 cut(s) 83, 790, 1357, 1720, 1853
BspCNI CTCAG 8 cut(s) 113, 160, 715, 926, 928, 1333, 1525, 1762
BspDI ATCGAT 2 cut(s) 1485, 1616
BspFNI CGCG 5 cut(s) 355, 389, 435, 1370, 1372
BspHI TCATGA 1 cut(s) 1822
BspLI GGNNCC 6 cut(s) 19, 762, 1377, 1680, 1705, 1720
BspMAI CTGCAG 1 cut(s) 934
BspPI GGATC 3 cut(s) 1612, 1820, 1894
BspQI GCTCTTC 1 cut(s) 321
BspT104I TTCGAA 1 cut(s) 1847
BspT107I GGYRCC 1 cut(s) 1375
BsrDI GCAATG 2 cut(s) 1795, 1812
BsrFI RCCGGY 2 cut(s) 475, 1094
BsrGI TGTACA 1 cut(s) 1419
BsrI ACTGG 4 cut(s) 297, 438, 1065, 1187
BssAI RCCGGY 2 cut(s) 475, 1094
BssECI CCNNGG 6 cut(s) 78, 756, 996, 1622, 1674, 1707
BssHII GCGCGC 1 cut(s) 1368
BssMI GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
BssT1I CCWWGG 5 cut(s) 756, 996, 1622, 1674, 1707
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 4 cut(s) 73, 547, 568, 1631
Bst6I CTCTTC 2 cut(s) 321, 1045
BstAPI GCANNNNNTGC 2 cut(s) 1790, 1814
BstAUI TGTACA 1 cut(s) 1419
BstBI TTCGAA 1 cut(s) 1847
BstC8I GCNNGC 9 cut(s) 85, 357, 709, 782, 975, 1370, 1374, 1786, 1862
BstDSI CCRYGG 1 cut(s) 1707
BstF5I GGATG 1 cut(s) 257
BstFNI CGCG 5 cut(s) 355, 389, 435, 1370, 1372
BstHHI GCGC 6 cut(s) 391, 437, 1370, 1372, 1497, 1613
BstKTI GATC 7 cut(s) 573, 613, 825, 1243, 1620, 1828, 1902
BstMAI GTCTC 1 cut(s) 1229
BstMBI GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
BstMWI GCNNNNNNNGC 8 cut(s) 38, 875, 979, 1397, 1712, 1785, 1790, 1814
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 1333
BstSFI CTRYAG 2 cut(s) 534, 930
BstSLI GKGCMC 3 cut(s) 1378, 1602, 1722
BstUI CGCG 5 cut(s) 355, 389, 435, 1370, 1372
BstV1I GCAGC 4 cut(s) 50, 878, 944, 1378
BstV2I GAAGAC 3 cut(s) 144, 801, 906
BstX2I RGATCY 1 cut(s) 1899
BstYI RGATCY 1 cut(s) 1899
Bsu15I ATCGAT 2 cut(s) 1485, 1616
BsuRI GGCC 5 cut(s) 83, 790, 1357, 1720, 1853
BsuTUI ATCGAT 2 cut(s) 1485, 1616
BtgI CCRYGG 1 cut(s) 1707
BtgZI GCGATG 1 cut(s) 314
BtrI CACGTC 1 cut(s) 1223
BtsCI GGATG 1 cut(s) 257
BtsI GCAGTG 3 cut(s) 646, 834, 1197
BtsIMutI CAGTG 4 cut(s) 646, 834, 1197, 1767
Cac8I GCNNGC 9 cut(s) 85, 357, 709, 782, 975, 1370, 1374, 1786, 1862
CciI TCATGA 1 cut(s) 1822
CfoI GCGC 6 cut(s) 391, 437, 1370, 1372, 1497, 1613
Cfr10I RCCGGY 2 cut(s) 475, 1094
Cfr13I GGNCC 4 cut(s) 82, 1678, 1718, 1719
ClaI ATCGAT 2 cut(s) 1485, 1616
CseI GACGC 2 cut(s) 395, 1280
CsiI ACCWGGT 1 cut(s) 1333
Csp6I GTAC 4 cut(s) 143, 184, 1420, 1639
CspAI ACCGGT 1 cut(s) 475
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 8 cut(s) 452, 614, 799, 1077, 1300, 1564, 1708, 1823
CviQI GTAC 4 cut(s) 143, 184, 1420, 1639
DpnI GATC 7 cut(s) 572, 612, 824, 1242, 1619, 1827, 1901
DpnII GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
DraI TTTAAA 1 cut(s) 895
DraIII CACNNNGTG 1 cut(s) 1337
Eam1104I CTCTTC 2 cut(s) 321, 1045
EarI CTCTTC 2 cut(s) 321, 1045
EciI GGCGGA 1 cut(s) 865
Eco130I CCWWGG 5 cut(s) 756, 996, 1622, 1674, 1707
Eco147I AGGCCT 2 cut(s) 790, 1357
Eco24I GRGCYC 1 cut(s) 1722
Eco47I GGWCC 1 cut(s) 1678
Eco57I CTGAAG 2 cut(s) 666, 813
EcoO109I RGGNCCY 2 cut(s) 1678, 1718
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 1333
EcoT14I CCWWGG 5 cut(s) 756, 996, 1622, 1674, 1707
EcoT22I ATGCAT 2 cut(s) 804, 910
EcoT38I GRGCYC 1 cut(s) 1722
ErhI CCWWGG 5 cut(s) 756, 996, 1622, 1674, 1707
Esp3I CGTCTC 1 cut(s) 1229
FaeI CATG 8 cut(s) 455, 617, 802, 1080, 1303, 1567, 1711, 1826
FalI AAGNNNNNCTT 4 cut(s) 512, 544, 1345, 1377
FaqI GGGAC 3 cut(s) 714, 1640, 1766
FatI CATG 8 cut(s) 451, 613, 798, 1076, 1299, 1563, 1707, 1822
FauI CCCGC 2 cut(s) 92, 1365
FbaI TGATCA 1 cut(s) 610
Fnu4HI GCNGC 6 cut(s) 39, 867, 879, 933, 1392, 1806
FokI GGATG 1 cut(s) 244
FriOI GRGCYC 1 cut(s) 1722
Fsp4HI GCNGC 6 cut(s) 39, 867, 879, 933, 1392, 1806
FspI TGCGCA 1 cut(s) 1496
GlaI GCGC 6 cut(s) 390, 436, 1369, 1371, 1496, 1612
GluI GCNGC 6 cut(s) 39, 867, 879, 933, 1392, 1806
HaeIII GGCC 5 cut(s) 83, 790, 1357, 1720, 1853
HapII CCGG 5 cut(s) 422, 476, 1095, 1452, 1903
HgaI GACGC 2 cut(s) 395, 1280
HhaI GCGC 6 cut(s) 391, 437, 1370, 1372, 1497, 1613
Hin1II CATG 8 cut(s) 455, 617, 802, 1080, 1303, 1567, 1711, 1826
Hin6I GCGC 6 cut(s) 389, 435, 1368, 1370, 1495, 1611
HinP1I GCGC 6 cut(s) 389, 435, 1368, 1370, 1495, 1611
HinfI GANTC 8 cut(s) 106, 167, 346, 481, 503, 1019, 1248, 1259
HpaII CCGG 5 cut(s) 422, 476, 1095, 1452, 1903
HphI GGTGA 3 cut(s) 208, 490, 614
Hpy166II GTNNAC 4 cut(s) 406, 473, 1600, 1767
Hpy188III TCNNGA 6 cut(s) 444, 915, 1112, 1238, 1382, 1823
Hpy8I GTNNAC 4 cut(s) 406, 473, 1600, 1767
Hpy99I CGWCG 1 cut(s) 1727
HpyAV CCTTC 5 cut(s) 186, 348, 722, 1448, 1843
HpyCH4III ACNGT 4 cut(s) 73, 547, 568, 1631
HpyCH4IV ACGT 3 cut(s) 238, 377, 1222
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 875, 979, 1397, 1712, 1785, 1790, 1814
HpySE526I ACGT 3 cut(s) 238, 377, 1222
Hsp92II CATG 8 cut(s) 455, 617, 802, 1080, 1303, 1567, 1711, 1826
HspAI GCGC 6 cut(s) 389, 435, 1368, 1370, 1495, 1611
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 3 cut(s) 986, 1350, 1703
Lsp1109I GCAGC 4 cut(s) 50, 878, 944, 1378
LweI GCATC 8 cut(s) 41, 266, 334, 742, 994, 1283, 1409, 1622
MabI ACCWGGT 1 cut(s) 1333
MaeII ACGT 3 cut(s) 238, 377, 1222
MaeIII GTNAC 3 cut(s) 547, 925, 958
MalI GATC 7 cut(s) 572, 612, 824, 1242, 1619, 1827, 1901
MboI GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
MflI RGATCY 1 cut(s) 1899
MhlI GDGCHC 3 cut(s) 1378, 1602, 1722
MluI ACGCGT 1 cut(s) 353
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 4 cut(s) 92, 542, 715, 1613
Mph1103I ATGCAT 2 cut(s) 804, 910
MseI TTAA 3 cut(s) 725, 894, 1467
MslI CAYNNNNRTG 2 cut(s) 1298, 1487
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 5 cut(s) 422, 476, 1095, 1452, 1903
MspR9I CCNGG 1 cut(s) 1335
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 5 cut(s) 355, 389, 435, 1370, 1372
MwoI GCNNNNNNNGC 8 cut(s) 38, 875, 979, 1397, 1712, 1785, 1790, 1814
NcoI CCATGG 1 cut(s) 1707
NdeII GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
NlaIII CATG 8 cut(s) 455, 617, 802, 1080, 1303, 1567, 1711, 1826
NlaIV GGNNCC 6 cut(s) 19, 762, 1377, 1680, 1705, 1720
NsbI TGCGCA 1 cut(s) 1496
NsiI ATGCAT 2 cut(s) 804, 910
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 1847
PagI TCATGA 1 cut(s) 1822
PauI GCGCGC 1 cut(s) 1368
PceI AGGCCT 2 cut(s) 790, 1357
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 579, 1095
PfeI GAWTC 7 cut(s) 167, 346, 481, 503, 1019, 1248, 1259
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 6 cut(s) 40, 868, 880, 934, 1393, 1807
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
PpuMI RGGWCCY 1 cut(s) 1678
PshBI ATTAAT 1 cut(s) 1467
Psp1406I AACGTT 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 1678
Psp6I CCWGG 1 cut(s) 1333
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 6 cut(s) 19, 762, 1377, 1680, 1705, 1720
PspOMI GGGCCC 1 cut(s) 1718
PspPI GGNCC 4 cut(s) 82, 1678, 1718, 1719
PspPPI RGGWCCY 1 cut(s) 1678
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 1 cut(s) 1899
PteI GCGCGC 1 cut(s) 1368
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 4 cut(s) 144, 185, 1421, 1640
RsaNI GTAC 4 cut(s) 143, 184, 1420, 1639
RseI CAYNNNNRTG 2 cut(s) 1298, 1487
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 725, 894, 1467
SatI GCNGC 6 cut(s) 39, 867, 879, 933, 1392, 1806
Sau3AI GATC 7 cut(s) 570, 610, 822, 1240, 1617, 1825, 1899
Sau96I GGNCC 4 cut(s) 82, 1678, 1718, 1719
ScaI AGTACT 1 cut(s) 1640
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 3 cut(s) 1378, 1602, 1722
SexAI ACCWGGT 1 cut(s) 1333
SfaNI GCATC 8 cut(s) 41, 266, 334, 742, 994, 1283, 1409, 1622
SfcI CTRYAG 2 cut(s) 534, 930
SfuI TTCGAA 1 cut(s) 1847
SinI GGWCC 1 cut(s) 1678
SmiMI CAYNNNNRTG 2 cut(s) 1298, 1487
SpeI ACTAGT 1 cut(s) 922
SseBI AGGCCT 2 cut(s) 790, 1357
SsiI CCGC 6 cut(s) 85, 876, 879, 1208, 1372, 1805
StuI AGGCCT 2 cut(s) 790, 1357
StyD4I CCNGG 1 cut(s) 1333
StyI CCWWGG 5 cut(s) 756, 996, 1622, 1674, 1707
TaaI ACNGT 4 cut(s) 73, 547, 568, 1631
TaiI ACGT 3 cut(s) 241, 380, 1225
TaqI TCGA 6 cut(s) 573, 821, 1383, 1485, 1616, 1847
TatI WGTACW 2 cut(s) 1419, 1638
TauI GCSGC 2 cut(s) 881, 1808
TfiI GAWTC 7 cut(s) 167, 346, 481, 503, 1019, 1248, 1259
Tru1I TTAA 3 cut(s) 725, 894, 1467
Tru9I TTAA 3 cut(s) 725, 894, 1467
TscAI CASTG 4 cut(s) 646, 841, 1204, 1774
TseI GCWGC 4 cut(s) 38, 866, 932, 1391
TspDTI ATGAA 8 cut(s) 679, 873, 1071, 1095, 1272, 1565, 1761, 1811
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 4 cut(s) 646, 841, 1204, 1774
VneI GTGCAC 1 cut(s) 1598
VpaK11BI GGWCC 1 cut(s) 1678
VspI ATTAAT 1 cut(s) 1467
XapI RAATTY 6 cut(s) 200, 428, 460, 721, 1027, 1690
XbaI TCTAGA 1 cut(s) 1111
XceI RCATGY 1 cut(s) 802
ZrmI AGTACT 1 cut(s) 1640
Zsp2I ATGCAT 2 cut(s) 804, 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.