Rroxscaffold_3G00268490

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011561
Physical Location & Seq
Reverse (-)
61574765 .. 61577161
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268490.1

Sequence Viewer

Length: 1803 bp
ATGCCATCTTCACTAGGTTACCAGTCACCTGCAACAGACCAAGAACCCGTGATGTTTGTCCATACAGTTTCGGAGGCACGTCGTAAGCTTCCTTCAGCAAGTCGCTGGAACTCTATAGAAATTGACTTCAATCTTGTTCCTCAATCTGGCATCGCCTATGATTCACTGCCTTCTGGTTACTCCAAGTCCTATGATTACAATATTGTTATTAGAGATAGAAAGCTCTTCAAACGTTGTCTTCACATTTCTGCCTCAATAGTTAGTCTTATCTTAGCTCTAATTTTGCTGCTACTGTTTTTGCCTCACAAGCATCATCACCATGGTCCTTCAAAGAACCTGACACTTGCATTGAACCAAGCTCTAACCTTCTTTGATGCTCAAAAATCTGGGACTTACCCCAAAAATAGTCCAGTAAAATTTCGAGGGGATTCAGGATTGCAAGATGGGAATTTGACCAGTAACAGCGCAAATCTTGTGGGCGGTTTCTATGATTCTGGAAACAACATGAAGTTCAGTTTTTCTACAGCTTATACCATGACCGTGTTAAGTTGGACTGTGATTGAATATCACCAAAAGTATGCTGATATCGGTGAGCTTGACCATGTCAAGGATATAATCAAATGGGGCAGTGATTATTTGCTCAAAATTGGAACTGCAAACAGTGATGGCACGACTCCTAGTGATATAAACTGCTGGGAAAGACCTGAAGACATGGATTATCAGAGACCTGTTTCAATTTGTGATAGTACAGCTACCGATTTAGCAGGGGAAATTGTTGCAGCATTATCTGCTGCTTCATTAGTATTTAAAGACGACAACAGTTTTTTCTCAGGAGAATTAGTGAAAGCAGCTGGGGAGTTATTTGAGAGTGCAACTAAAGACCCTAATAATCAAGGAACTTACACCAGGGTTGAAAGTTGTGGAGGAGAATCTAGAGACTTTTATAACTCATCTGGTTATAAAGATGAATTAGTTTGGGGAGGAACTTGGTTGTTTTTTGCTACTGGTGACATTTCGTATCTGCAATATGCCACAGACAAGTTTCAGGCTGCAGTTGATGATGAAACAAGTTCTGATAAATGGATTTTCGATTGGAACAACAAGCTTACTGCTAACATGGTCTTGCTAACGCGTCTTCAATTCTTTCATGATCTTGGCTACCCGTATGAAGATTCTTTAAAGGCATCATCCAACATGACTGGCTCCCTTATGTGTTCTTATGTTTCTCATCGAGGCTTCAGCATGACACCAGGTGGGTTGGTCATCCAAAGGCCTGATTATGGGGCACCTCTCCAGTTTGCTGCAACAGCATCTTTCCTTAGTAAATTGTACGGCGACTACCTTGATCTTCTGCGTAGTTCTGGTGGCAGTTGCAGCACTTGTGAATTCACTGTGAATTACATTCTAGGAGATAATCCAATGAATATGAGCTACATGGTGGGATTCGGGGACAAGTTTCCGAGCCAAGTACACCATAGAAGTGCATCAATCCCTTGGGATGGTCAACATTACTCGTGTAAAGATGGATATAAATGGCGAGACTCCAAGGATCCAAATCCGAATGTGCTGTTGGGAGCCATGGTAGGAGGACCAGACCAGTTTGACAAATTTATAGATGAGAGGGACAAGCAAGAGTTCACTGAGCCTAGATTAACAAGCAATGCTGGTTTAGTTGCAGCTCTCATTGCACTTCATGATCCTCCTCATCCTAAGAAGAGCAAGAAGAGAGGCAATTATGATCCCACTAAATTGGGCATAGACCAGATGGGTATTTTTGATAAGATTGTACCCCTAGCCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

600

Amino Acids

66.66

Weight (kDa)

5.64

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 116 - 561 1.5e-118 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 945, 960
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 1285
AccII CGCG 1 cut(s) 1132
AciI CCGC 1 cut(s) 480
AclI AACGTT 1 cut(s) 232
AclWI GGATC 4 cut(s) 1544, 1557, 1691, 1733
AcsI RAATTY 4 cut(s) 416, 448, 1385, 1607
AcuI CTGAAG 3 cut(s) 78, 726, 1222
AdeI CACNNNGTG 1 cut(s) 1253
AfaI GTAC 4 cut(s) 748, 1331, 1470, 1788
AfiI CCNNNNNNNGG 4 cut(s) 146, 607, 1280, 1499
AflIII ACRYGT 1 cut(s) 1130
AgsI TTSAA 8 cut(s) 130, 229, 330, 352, 563, 735, 914, 1139
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 2 cut(s) 905, 1249
AjuI GAANNNNNNNTTGG 2 cut(s) 1553, 1585
Alw26I GTCTC 3 cut(s) 718, 930, 1533
AlwI GGATC 4 cut(s) 1544, 1557, 1691, 1733
AoxI GGCC 1 cut(s) 1271
ApeKI GCWGC 8 cut(s) 286, 779, 791, 848, 1049, 1301, 1374, 1676
ApoI RAATTY 4 cut(s) 416, 448, 1385, 1607
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 323, 1589
AsuHPI GGTGA 5 cut(s) 18, 308, 560, 602, 1019
AvaII GGWCC 2 cut(s) 323, 1589
BaeGI GKGCMC 1 cut(s) 1288
BamHI GGATCC 1 cut(s) 1549
BanI GGYRCC 1 cut(s) 1285
BarI GAAGNNNNNNTAC 2 cut(s) 76, 108
BauI CACGAG 1 cut(s) 1513
BbsI GAAGAC 3 cut(s) 230, 714, 1127
BbvI GCAGC 8 cut(s) 273, 778, 791, 860, 1036, 1288, 1386, 1688
BccI CCATC 6 cut(s) 13, 437, 659, 1493, 1517, 1759
BceAI ACGGC 1 cut(s) 1348
BciT130I CCWGG 2 cut(s) 907, 1251
BcoDI GTCTC 3 cut(s) 718, 930, 1533
BfaI CTAG 6 cut(s) 14, 678, 933, 1406, 1647, 1793
BfmI CTRYAG 3 cut(s) 114, 522, 1050
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 8 cut(s) 287, 780, 792, 849, 1050, 1302, 1375, 1677
BlsI GCNGC 8 cut(s) 288, 781, 793, 850, 1051, 1303, 1376, 1678
Bme1390I CCNGG 2 cut(s) 907, 1251
Bme18I GGWCC 2 cut(s) 323, 1589
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 323, 1589
BmiI GGNNCC 4 cut(s) 1204, 1287, 1551, 1576
BmrFI CCNGG 2 cut(s) 907, 1251
BmsI GCATC 6 cut(s) 159, 319, 364, 1193, 1319, 1493
BpiI GAAGAC 3 cut(s) 230, 714, 1127
BpmI CTGGAG 1 cut(s) 1277
BsaBI GATNNNNATC 1 cut(s) 1554
BsaI GGTCTC 1 cut(s) 718
BsaJI CCNNGG 5 cut(s) 319, 906, 1493, 1545, 1578
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 1566, 1596
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 607, 1280, 1499
Bse1I ACTGG 7 cut(s) 22, 410, 456, 1009, 1204, 1294, 1597
Bse3DI GCAATG 2 cut(s) 1666, 1683
Bse8I GATNNNNATC 1 cut(s) 1554
BseBI CCWGG 2 cut(s) 907, 1251
BseDI CCNNGG 5 cut(s) 319, 906, 1493, 1545, 1578
BseGI GGATG 4 cut(s) 1187, 1263, 1504, 1705
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 4 cut(s) 146, 607, 1280, 1499
BseMI GCAATG 2 cut(s) 1666, 1683
BseMII CTCAG 2 cut(s) 843, 1632
BseNI ACTGG 7 cut(s) 22, 410, 456, 1009, 1204, 1294, 1597
BseRI GAGGAG 2 cut(s) 939, 1692
BseSI GKGCMC 1 cut(s) 1288
BseXI GCAGC 8 cut(s) 273, 778, 791, 860, 1036, 1288, 1386, 1688
BseYI CCCAGC 2 cut(s) 693, 851
Bsh1236I CGCG 1 cut(s) 1132
BshFI GGCC 1 cut(s) 1273
BshNI GGYRCC 1 cut(s) 1285
BslFI GGGAC 3 cut(s) 403, 1463, 1637
BslI CCNNNNNNNGG 4 cut(s) 146, 607, 1280, 1499
BsmAI GTCTC 3 cut(s) 718, 930, 1533
BsmFI GGGAC 3 cut(s) 403, 1463, 1637
BsnI GGCC 1 cut(s) 1273
Bso31I GGTCTC 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 1288
Bsp143I GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
Bsp19I CCATGG 2 cut(s) 319, 1578
BspACI CCGC 1 cut(s) 480
BspANI GGCC 1 cut(s) 1273
BspCNI CTCAG 2 cut(s) 842, 1633
BspFNI CGCG 1 cut(s) 1132
BspHI TCATGA 2 cut(s) 1147, 1693
BspLI GGNNCC 4 cut(s) 1204, 1287, 1551, 1576
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 4 cut(s) 1544, 1557, 1691, 1733
BspQI GCTCTTC 2 cut(s) 230, 1709
BspT107I GGYRCC 1 cut(s) 1285
BspTNI GGTCTC 1 cut(s) 718
BsrDI GCAATG 2 cut(s) 1666, 1683
BsrI ACTGG 7 cut(s) 22, 410, 456, 1009, 1204, 1294, 1597
BssECI CCNNGG 5 cut(s) 319, 906, 1493, 1545, 1578
BssMI GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
BssSI CACGAG 1 cut(s) 1513
BssT1I CCWWGG 4 cut(s) 319, 1493, 1545, 1578
Bst2BI CACGAG 1 cut(s) 1513
Bst2UI CCWGG 2 cut(s) 907, 1251
Bst4CI ACNGT 7 cut(s) 67, 294, 541, 556, 662, 821, 1393
Bst6I CTCTTC 3 cut(s) 230, 1709, 1718
BstAPI GCANNNNNTGC 1 cut(s) 788
BstDEI CTNAG 5 cut(s) 271, 829, 1319, 1641, 1710
BstDSI CCRYGG 2 cut(s) 319, 1578
BstEII GGTNACC 1 cut(s) 17
BstF5I GGATG 4 cut(s) 1187, 1263, 1504, 1705
BstFNI CGCG 1 cut(s) 1132
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 5 cut(s) 1153, 1348, 1552, 1699, 1741
BstMAI GTCTC 3 cut(s) 718, 930, 1533
BstMBI GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
BstMWI GCNNNNNNNGC 5 cut(s) 307, 788, 1307, 1374, 1685
BstNI CCWGG 2 cut(s) 907, 1251
BstPI GGTNACC 1 cut(s) 17
BstSCI CCNGG 2 cut(s) 905, 1249
BstSFI CTRYAG 3 cut(s) 114, 522, 1050
BstSLI GKGCMC 1 cut(s) 1288
BstUI CGCG 1 cut(s) 1132
BstV1I GCAGC 8 cut(s) 273, 778, 791, 860, 1036, 1288, 1386, 1688
BstV2I GAAGAC 3 cut(s) 230, 714, 1127
BstX2I RGATCY 1 cut(s) 1549
BstXI CCANNNNNNTGG 1 cut(s) 1750
BstYI RGATCY 1 cut(s) 1549
BsuRI GGCC 1 cut(s) 1273
BtgI CCRYGG 2 cut(s) 319, 1578
BtgZI GCGATG 1 cut(s) 136
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 4 cut(s) 1187, 1263, 1504, 1705
BtsI GCAGTG 2 cut(s) 164, 634
BtsIMutI CAGTG 5 cut(s) 164, 634, 667, 1389, 1638
BveI ACCTGC 1 cut(s) 37
CciI TCATGA 2 cut(s) 1147, 1693
CfoI GCGC 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 323, 1589
CseI GACGC 1 cut(s) 1121
CsiI ACCWGGT 1 cut(s) 1249
Csp6I GTAC 4 cut(s) 747, 1330, 1469, 1787
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviQI GTAC 4 cut(s) 747, 1330, 1469, 1787
DdeI CTNAG 5 cut(s) 271, 829, 1319, 1641, 1710
DpnI GATC 5 cut(s) 1152, 1347, 1551, 1698, 1740
DpnII GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
DraI TTTAAA 2 cut(s) 808, 1179
DraIII CACNNNGTG 1 cut(s) 1253
Eam1104I CTCTTC 3 cut(s) 230, 1709, 1718
EarI CTCTTC 3 cut(s) 230, 1709, 1718
Eco130I CCWWGG 4 cut(s) 319, 1493, 1545, 1578
Eco147I AGGCCT 1 cut(s) 1273
Eco31I GGTCTC 1 cut(s) 718
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 323, 1589
Eco57I CTGAAG 3 cut(s) 78, 726, 1222
Eco91I GGTNACC 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 2 cut(s) 905, 1249
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 4 cut(s) 319, 1493, 1545, 1578
ErhI CCWWGG 4 cut(s) 319, 1493, 1545, 1578
FaqI GGGAC 3 cut(s) 403, 1463, 1637
Fnu4HI GCNGC 8 cut(s) 287, 780, 792, 849, 1050, 1302, 1375, 1677
FokI GGATG 4 cut(s) 1174, 1250, 1511, 1692
Fsp4HI GCNGC 8 cut(s) 287, 780, 792, 849, 1050, 1302, 1375, 1677
FspBI CTAG 6 cut(s) 14, 678, 933, 1406, 1647, 1793
GlaI GCGC 1 cut(s) 466
GluI GCNGC 8 cut(s) 287, 780, 792, 849, 1050, 1302, 1375, 1677
GsaI CCCAGC 2 cut(s) 697, 855
GsuI CTGGAG 1 cut(s) 1277
HaeIII GGCC 1 cut(s) 1273
HgaI GACGC 1 cut(s) 1121
HhaI GCGC 1 cut(s) 467
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HincII GTYRAC 1 cut(s) 1505
HindII GTYRAC 1 cut(s) 1505
HindIII AAGCTT 2 cut(s) 86, 1103
HinfI GANTC 8 cut(s) 161, 428, 491, 673, 929, 1172, 1443, 1541
HphI GGTGA 5 cut(s) 18, 308, 560, 602, 1019
Hpy166II GTNNAC 3 cut(s) 1471, 1505, 1638
Hpy188I TCNGA 5 cut(s) 73, 723, 1075, 1461, 1560
Hpy188III TCNNGA 6 cut(s) 432, 495, 831, 933, 1148, 1694
Hpy8I GTNNAC 3 cut(s) 1471, 1505, 1638
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 4 cut(s) 102, 180, 336, 376
HpyCH4III ACNGT 7 cut(s) 67, 294, 541, 556, 662, 821, 1393
HpyCH4IV ACGT 2 cut(s) 79, 232
HpyF10VI GCNNNNNNNGC 5 cut(s) 307, 788, 1307, 1374, 1685
HpyF3I CTNAG 5 cut(s) 271, 829, 1319, 1641, 1710
HpySE526I ACGT 2 cut(s) 79, 232
HspAI GCGC 1 cut(s) 465
Kzo9I GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
LguI GCTCTTC 2 cut(s) 230, 1709
LmnI GCTCC 2 cut(s) 1208, 1574
Lsp1109I GCAGC 8 cut(s) 273, 778, 791, 860, 1036, 1288, 1386, 1688
LweI GCATC 6 cut(s) 159, 319, 364, 1193, 1319, 1493
MabI ACCWGGT 1 cut(s) 1249
MaeI CTAG 6 cut(s) 14, 678, 933, 1406, 1647, 1793
MaeII ACGT 2 cut(s) 79, 232
MaeIII GTNAC 5 cut(s) 17, 24, 176, 458, 1007
MalI GATC 5 cut(s) 1152, 1347, 1551, 1698, 1740
MboI GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
MboII GAAGA 8 cut(s) 217, 230, 719, 1127, 1181, 1340, 1726, 1735
MflI RGATCY 1 cut(s) 1549
MhlI GDGCHC 1 cut(s) 1288
MluI ACGCGT 1 cut(s) 1130
MlyI GAGTC 2 cut(s) 667, 1535
MmeI TCCRAC 2 cut(s) 530, 1215
MseI TTAA 5 cut(s) 545, 807, 1178, 1652, 1801
MslI CAYNNNNRTG 3 cut(s) 318, 539, 1479
MspA1I CMGCKG 1 cut(s) 851
MspR9I CCNGG 2 cut(s) 907, 1251
MvaI CCWGG 2 cut(s) 907, 1251
MvnI CGCG 1 cut(s) 1132
MwoI GCNNNNNNNGC 5 cut(s) 307, 788, 1307, 1374, 1685
NcoI CCATGG 2 cut(s) 319, 1578
NdeII GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
NlaIV GGNNCC 4 cut(s) 1204, 1287, 1551, 1576
NmuCI GTSAC 2 cut(s) 24, 1007
PagI TCATGA 2 cut(s) 1147, 1693
PaqCI CACCTGC 1 cut(s) 37
PceI AGGCCT 1 cut(s) 1273
PciSI GCTCTTC 2 cut(s) 230, 1709
PfeI GAWTC 6 cut(s) 161, 428, 491, 929, 1172, 1443
PflFI GACNNNGTC 1 cut(s) 602
PkrI GCNGC 8 cut(s) 288, 781, 793, 850, 1051, 1303, 1376, 1678
PleI GAGTC 2 cut(s) 667, 1535
PpsI GAGTC 2 cut(s) 667, 1535
PsiI TTATAA 2 cut(s) 945, 960
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 905, 1249
PspEI GGTNACC 1 cut(s) 17
PspFI CCCAGC 2 cut(s) 693, 851
PspGI CCWGG 2 cut(s) 905, 1249
PspN4I GGNNCC 4 cut(s) 1204, 1287, 1551, 1576
PspPI GGNCC 2 cut(s) 323, 1589
PstI CTGCAG 1 cut(s) 1054
PsuI RGATCY 1 cut(s) 1549
PsyI GACNNNGTC 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 4 cut(s) 748, 1331, 1470, 1788
RsaNI GTAC 4 cut(s) 747, 1330, 1469, 1787
RseI CAYNNNNRTG 3 cut(s) 318, 539, 1479
SapI GCTCTTC 2 cut(s) 230, 1709
SaqAI TTAA 5 cut(s) 545, 807, 1178, 1652, 1801
SatI GCNGC 8 cut(s) 287, 780, 792, 849, 1050, 1302, 1375, 1677
Sau3AI GATC 5 cut(s) 1150, 1345, 1549, 1696, 1738
Sau96I GGNCC 2 cut(s) 323, 1589
SchI GAGTC 2 cut(s) 667, 1535
ScrFI CCNGG 2 cut(s) 907, 1251
SduI GDGCHC 1 cut(s) 1288
SexAI ACCWGGT 1 cut(s) 1249
SfaNI GCATC 6 cut(s) 159, 319, 364, 1193, 1319, 1493
SfcI CTRYAG 3 cut(s) 114, 522, 1050
SinI GGWCC 2 cut(s) 323, 1589
SmiMI CAYNNNNRTG 3 cut(s) 318, 539, 1479
SseBI AGGCCT 1 cut(s) 1273
SsiI CCGC 1 cut(s) 480
SspI AATATT 1 cut(s) 202
SspMI CTAG 6 cut(s) 14, 678, 933, 1406, 1647, 1793
StuI AGGCCT 1 cut(s) 1273
StyD4I CCNGG 2 cut(s) 905, 1249
StyI CCWWGG 4 cut(s) 319, 1493, 1545, 1578
TaaI ACNGT 7 cut(s) 67, 294, 541, 556, 662, 821, 1393
TaiI ACGT 2 cut(s) 82, 235
TaqI TCGA 3 cut(s) 421, 1089, 1231
TatI WGTACW 2 cut(s) 746, 1468
TfiI GAWTC 6 cut(s) 161, 428, 491, 929, 1172, 1443
Tru1I TTAA 5 cut(s) 545, 807, 1178, 1652, 1801
Tru9I TTAA 5 cut(s) 545, 807, 1178, 1652, 1801
TscAI CASTG 5 cut(s) 171, 634, 667, 1396, 1645
TseFI GTSAC 2 cut(s) 24, 1007
TseI GCWGC 8 cut(s) 286, 779, 791, 848, 1049, 1301, 1374, 1676
Tsp45I GTSAC 2 cut(s) 24, 1007
TspDTI ATGAA 8 cut(s) 521, 786, 981, 1077, 1136, 1182, 1436, 1682
TspRI CASTG 5 cut(s) 171, 634, 667, 1396, 1645
Tth111I GACNNNGTC 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 323, 1589
XapI RAATTY 4 cut(s) 416, 448, 1385, 1607
XbaI TCTAGA 1 cut(s) 932
XspI CTAG 6 cut(s) 14, 678, 933, 1406, 1647, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.