FvH4_5g12901

Protein Dr1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009087
Physical Location & Seq
Reverse (-)
7284166 .. 7286728
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12901.t1

Sequence Viewer

Length: 471 bp
ATGGAGCCGATGGACATCGTCGGAAAGTCAAAAGAAGACGCTTCGCTTCCGAAAGCAACCATGACCAAGATCATTAAGGAGATGTTGCCTCCAGATGTGCGCGTCGCAAGAGATGCTCAAGATCTTCTCATTGAGTGTTGTGTAGAGTTTATAAATCTCATTTCATCAGAGTCGAATGAAGTTTGTAGTAGAGAGGAGAAAAGAACAATAGCTCCTGAGCACGTGCTCAAGGCTTTACAGGTCCTTGGGTTCAGCGAGTACATTGAGGAAGTTTATGCAGCATATGAACAACACAAGATTGAGACTGTGCATGACGTAGCTAAAAGTGTCAAATGGGGCAATGGAGCAGAGATGACCGAGGAACAAGCCTTGGCAGAGCAGCAGAGGATGTTTGCCGAGGCACGTGCCAGAATGAATGGAGGCGCAACTGCGCCCAAGCAACCAGACCCAGACCAAAGTTTAGAGAGCTAA

Protein Analysis

157

Amino Acids

17.44

Weight (kDa)

4.77

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 14 - 79 3e-22 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccII CGCG 1 cut(s) 102
AcvI CACGTG 2 cut(s) 223, 404
AfaI GTAC 1 cut(s) 260
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 3 cut(s) 212, 320, 468
AluI AGCT 3 cut(s) 212, 320, 468
Alw21I GWGCWC 2 cut(s) 222, 228
Alw26I GTCTC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 278, 379
AspLEI GCGC 3 cut(s) 102, 425, 433
AspS9I GGNCC 1 cut(s) 241
AvaII GGWCC 1 cut(s) 241
BbrPI CACGTG 2 cut(s) 223, 404
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 222, 228
BbvI GCAGC 2 cut(s) 290, 391
BccI CCATC 1 cut(s) 4
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BcoDI GTCTC 1 cut(s) 296
BglII AGATCT 1 cut(s) 121
BisI GCNGC 2 cut(s) 279, 380
BlsI GCNGC 2 cut(s) 280, 381
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 102, 212
BsaAI YACGTR 2 cut(s) 223, 404
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 4 cut(s) 244, 357, 369, 396
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bse3DI GCAATG 1 cut(s) 346
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 4 cut(s) 244, 357, 369, 396
BseGI GGATG 1 cut(s) 393
BseJI GATNNNNATC 1 cut(s) 14
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 290, 391
Bsh1236I CGCG 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 222, 228
BsmAI GTCTC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 222, 228
Bsp143I GATC 2 cut(s) 69, 121
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 244, 357, 369, 396
BssMI GATC 2 cut(s) 69, 121
BssT1I CCWWGG 2 cut(s) 244, 369
Bst4CI ACNGT 1 cut(s) 307
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 2 cut(s) 223, 404
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 3 cut(s) 102, 425, 433
BstKTI GATC 2 cut(s) 72, 124
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 69, 121
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 290, 391
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BtsCI GGATG 1 cut(s) 393
CfoI GCGC 3 cut(s) 102, 425, 433
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 2 cut(s) 47, 91
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 61, 311
CviJI RGCY 6 cut(s) 7, 212, 233, 320, 368, 468
CviKI_1 RGCY 6 cut(s) 7, 212, 233, 320, 368, 468
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 216
DpnI GATC 2 cut(s) 71, 123
DpnII GATC 2 cut(s) 69, 121
Eco130I CCWWGG 2 cut(s) 244, 369
Eco47I GGWCC 1 cut(s) 241
Eco72I CACGTG 2 cut(s) 223, 404
EcoO109I RGGNCCY 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 244, 369
ErhI CCWWGG 2 cut(s) 244, 369
FaeI CATG 2 cut(s) 64, 314
FaiI YATR 6 cut(s) 62, 152, 276, 283, 285, 312
FatI CATG 2 cut(s) 60, 310
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 279, 380
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 2 cut(s) 279, 380
GlaI GCGC 3 cut(s) 101, 424, 432
GluI GCNGC 2 cut(s) 279, 380
GsuI CTGGAG 1 cut(s) 75
HgaI GACGC 2 cut(s) 47, 91
HhaI GCGC 3 cut(s) 102, 425, 433
Hin1II CATG 2 cut(s) 64, 314
Hin6I GCGC 3 cut(s) 100, 423, 431
HinP1I GCGC 3 cut(s) 100, 423, 431
HinfI GANTC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 23, 51, 169
Hpy188III TCNNGA 3 cut(s) 92, 119, 215
Hpy99I CGWCG 2 cut(s) 23, 107
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4IV ACGT 3 cut(s) 222, 315, 403
HpyCH4V TGCA 2 cut(s) 278, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 3 cut(s) 222, 315, 403
Hsp92II CATG 2 cut(s) 64, 314
HspAI GCGC 3 cut(s) 100, 423, 431
Kzo9I GATC 2 cut(s) 69, 121
LmnI GCTCC 3 cut(s) 4, 217, 344
LpnPI CCDG 6 cut(s) 105, 224, 228, 421, 456, 462
Lsp1109I GCAGC 2 cut(s) 290, 391
LweI GCATC 1 cut(s) 103
MaeII ACGT 3 cut(s) 222, 315, 403
MalI GATC 2 cut(s) 71, 123
MboI GATC 2 cut(s) 69, 121
MboII GAAGA 2 cut(s) 47, 116
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 222, 228
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 7 cut(s) 99, 187, 259, 352, 378, 391, 413
MseI TTAA 1 cut(s) 75
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 113
NdeI CATATG 1 cut(s) 283
NdeII GATC 2 cut(s) 69, 121
NlaIII CATG 2 cut(s) 64, 314
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 421
PflFI GACNNNGTC 1 cut(s) 17
PkrI GCNGC 2 cut(s) 280, 381
PleI GAGTC 1 cut(s) 178
PmaCI CACGTG 2 cut(s) 223, 404
PmlI CACGTG 2 cut(s) 223, 404
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 2 cut(s) 223, 404
PpuMI RGGWCCY 1 cut(s) 241
PsiI TTATAA 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 241
PspCI CACGTG 2 cut(s) 223, 404
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 121
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 2 cut(s) 279, 380
Sau3AI GATC 2 cut(s) 69, 121
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 2 cut(s) 222, 228
SetI ASST 7 cut(s) 214, 225, 243, 318, 322, 406, 470
SfaNI GCATC 1 cut(s) 103
SinI GGWCC 1 cut(s) 241
SmlI CTYRAG 2 cut(s) 117, 227
SmoI CTYRAG 2 cut(s) 117, 227
StyI CCWWGG 2 cut(s) 244, 369
TaaI ACNGT 1 cut(s) 307
TaiI ACGT 3 cut(s) 225, 318, 406
TaqI TCGA 1 cut(s) 173
TaqII GACCGA 1 cut(s) 371
TatI WGTACW 1 cut(s) 258
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseI GCWGC 2 cut(s) 278, 379
TspDTI ATGAA 4 cut(s) 153, 192, 300, 428
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.