MD14G1219800.v1.1

Protein Dr1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009087
Physical Location & Seq
Forward (+)
30211443 .. 30214194
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1219800.v1.1.491

Sequence Viewer

Length: 471 bp
ATGGATCCGATGGACATCGTCGGGAAGTCAAAGGAAGATGCTTCGCTTCCGAAAGCAACCATGACCAAAATCATTAAAGAGATGCTGCCGCCAGATGTACGTGTCGCAAGAGATGCTCAAGATCTTTTGATTGAGTGTTGTGTAGAGTTTATAAATCTCATCTCCTCAGAGTCGAATGAAGTTTGTGGCAGAGAGGAGAAAAGAACAATAGCTCCTGAGCATGTACTCAAGGCTTTACAGGTTCTTGGGTTTAGCGAGTACATTGAGGAAGTTTATTCAGCGTATGAACAACACAAACTGGAGACTATGCATGACCTATCAAAAAGTGTCAAATGGGGGAACGGAGTGGAGATGACAGAGGAAGAAGCCTTGGCTGAGCAGCAGAGGATGTTTGCCGAGGCACGTGCCAGAATGAATGGTGGAGCCACCGCGCCCAAGCAACCAGACCCCGAACAAAGTTTAGAGAGCTAA

Protein Analysis

157

Amino Acids

17.51

Weight (kDa)

4.72

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 14 - 79 5.7e-22 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccII CGCG 1 cut(s) 431
AciI CCGC 2 cut(s) 89, 429
AclWI GGATC 1 cut(s) 12
AcvI CACGTG 1 cut(s) 404
AfaI GTAC 3 cut(s) 99, 225, 260
AflIII ACRYGT 1 cut(s) 100
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 2 cut(s) 212, 468
AluI AGCT 2 cut(s) 212, 468
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 85, 379
AspLEI GCGC 1 cut(s) 433
BamHI GGATCC 1 cut(s) 4
BbrPI CACGTG 1 cut(s) 404
BbvI GCAGC 2 cut(s) 72, 391
BccI CCATC 1 cut(s) 4
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BcoDI GTCTC 1 cut(s) 296
BglII AGATCT 1 cut(s) 121
BisI GCNGC 3 cut(s) 86, 89, 380
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 3 cut(s) 87, 90, 381
BmiI GGNNCC 2 cut(s) 6, 424
BmsI GCATC 3 cut(s) 28, 72, 103
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 102, 212
BsaAI YACGTR 2 cut(s) 101, 404
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 369, 396
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bse1I ACTGG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 369, 396
BseGI GGATG 1 cut(s) 393
BseJI GATNNNNATC 1 cut(s) 14
BseMII CTCAG 3 cut(s) 180, 207, 366
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 2 cut(s) 154, 209
BseXI GCAGC 2 cut(s) 72, 391
Bsh1236I CGCG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 296
Bsp143I GATC 2 cut(s) 4, 121
Bsp1720I GCTNAGC 1 cut(s) 375
BspACI CCGC 2 cut(s) 89, 429
BspCNI CTCAG 3 cut(s) 179, 208, 367
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 6, 424
BspPI GGATC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 2 cut(s) 369, 396
BssMI GATC 2 cut(s) 4, 121
BssT1I CCWWGG 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 2 cut(s) 101, 404
BstDEI CTNAG 3 cut(s) 166, 216, 375
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 7, 124
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 4, 121
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 224
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 72, 391
BstX2I RGATCY 2 cut(s) 4, 121
BstYI RGATCY 2 cut(s) 4, 121
BtsCI GGATG 1 cut(s) 393
CfoI GCGC 1 cut(s) 433
Csp6I GTAC 3 cut(s) 98, 224, 259
CviAII CATG 3 cut(s) 61, 221, 311
CviJI RGCY 6 cut(s) 212, 233, 368, 374, 425, 468
CviKI_1 RGCY 6 cut(s) 212, 233, 368, 374, 425, 468
CviQI GTAC 3 cut(s) 98, 224, 259
DdeI CTNAG 3 cut(s) 166, 216, 375
DpnI GATC 2 cut(s) 6, 123
DpnII GATC 2 cut(s) 4, 121
Eco130I CCWWGG 1 cut(s) 369
Eco72I CACGTG 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 3 cut(s) 64, 224, 314
FaiI YATR 6 cut(s) 62, 152, 222, 285, 308, 312
FatI CATG 3 cut(s) 60, 220, 310
Fnu4HI GCNGC 3 cut(s) 86, 89, 380
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 3 cut(s) 86, 89, 380
GlaI GCGC 1 cut(s) 432
GluI GCNGC 3 cut(s) 86, 89, 380
GsuI CTGGAG 1 cut(s) 320
HhaI GCGC 1 cut(s) 433
Hin1II CATG 3 cut(s) 64, 224, 314
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HinfI GANTC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 9, 51, 169
Hpy188III TCNNGA 3 cut(s) 22, 119, 215
Hpy99I CGWCG 1 cut(s) 23
HpyCH4IV ACGT 2 cut(s) 100, 403
HpyCH4V TGCA 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 3 cut(s) 166, 216, 375
HpySE526I ACGT 2 cut(s) 100, 403
Hsp92II CATG 3 cut(s) 64, 224, 314
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 2 cut(s) 4, 121
LmnI GCTCC 2 cut(s) 217, 422
LpnPI CCDG 6 cut(s) 105, 224, 228, 284, 421, 456
Lsp1109I GCAGC 2 cut(s) 72, 391
LweI GCATC 3 cut(s) 28, 72, 103
MaeII ACGT 2 cut(s) 100, 403
MalI GATC 2 cut(s) 6, 123
MboI GATC 2 cut(s) 4, 121
MboII GAAGA 2 cut(s) 47, 374
MflI RGATCY 2 cut(s) 4, 121
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 6 cut(s) 175, 187, 259, 352, 378, 391
Mph1103I ATGCAT 1 cut(s) 312
MseI TTAA 1 cut(s) 75
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 113
NdeII GATC 2 cut(s) 4, 121
NlaIII CATG 3 cut(s) 64, 224, 314
NlaIV GGNNCC 2 cut(s) 6, 424
NmeAIII GCCGAG 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 1 cut(s) 224
PflFI GACNNNGTC 1 cut(s) 17
PkrI GCNGC 3 cut(s) 87, 90, 381
PleI GAGTC 1 cut(s) 178
PmaCI CACGTG 1 cut(s) 404
PmlI CACGTG 1 cut(s) 404
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 2 cut(s) 101, 404
PsiI TTATAA 1 cut(s) 152
PspCI CACGTG 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 6, 424
PsuI RGATCY 2 cut(s) 4, 121
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 3 cut(s) 99, 225, 260
RsaNI GTAC 3 cut(s) 98, 224, 259
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 3 cut(s) 86, 89, 380
Sau3AI GATC 2 cut(s) 4, 121
SchI GAGTC 1 cut(s) 179
SetI ASST 6 cut(s) 103, 214, 243, 318, 406, 470
SfaNI GCATC 3 cut(s) 28, 72, 103
SmlI CTYRAG 2 cut(s) 117, 227
SmoI CTYRAG 2 cut(s) 117, 227
SsiI CCGC 2 cut(s) 89, 429
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 2 cut(s) 103, 406
TaqI TCGA 1 cut(s) 173
TatI WGTACW 2 cut(s) 223, 258
TauI GCSGC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseI GCWGC 2 cut(s) 85, 379
TspDTI ATGAA 3 cut(s) 192, 300, 428
TspGWI ACGGA 1 cut(s) 357
Tth111I GACNNNGTC 1 cut(s) 17
XceI RCATGY 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.