pycom14g18210

Protein Dr1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009087
Physical Location & Seq
Forward (+)
19925430 .. 19926950
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18210.1

Sequence Viewer

Length: 390 bp
ATGTTGCCGCCAGATGTACGTGTCGCAAGAGATGCTCAAGATCTTTTGATTGAGTGTTGCGTAGAGTTTATAAATCTCATCTCATCAGAGTCGAATGAAGTTTGTAGCAGAGAGGAGAAAAGAACAATAGCCCCCGAGCATGTACTCAAGGCTTTACAGGTTCTTGGGTTTAGCGAGTACATTGAGGAAGTTTATTCAGCATATGAACAACACAAGCTTGAGACTATGCATGACTTATCAAAAAGTGTCAAATGGGGGAACGGAGTGGAGATGACAGAGGAAGAAGCCTTGGCTGAGCAACAGAGGATGTTTGCCGAGGCACGTGCCAGAATGAATGGTGGAGCCACTGCGCCCAAGCAACCAGACCCTGAACAAAGTTTAGAGAGCTAA

Protein Analysis

130

Amino Acids

14.58

Weight (kDa)

4.63

Isoelectric Point (pI)

65.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 4 - 52 1e-15 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AciI CCGC 1 cut(s) 8
AcvI CACGTG 1 cut(s) 323
AfaI GTAC 3 cut(s) 18, 144, 179
AflIII ACRYGT 1 cut(s) 19
AluBI AGCT 2 cut(s) 217, 387
AluI AGCT 2 cut(s) 217, 387
Alw26I GTCTC 1 cut(s) 215
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 134
AspLEI GCGC 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 134
BbrPI CACGTG 1 cut(s) 323
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BcoDI GTCTC 1 cut(s) 215
BglII AGATCT 1 cut(s) 40
BisI GCNGC 1 cut(s) 8
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 9
BmeT110I CYCGRG 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 343
BmsI GCATC 1 cut(s) 22
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 3 cut(s) 21, 131, 239
BsaAI YACGTR 2 cut(s) 20, 323
BsaJI CCNNGG 2 cut(s) 288, 315
BseDI CCNNGG 2 cut(s) 288, 315
BseGI GGATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 134
Bsp143I GATC 1 cut(s) 40
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 288, 315
BssMI GATC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 2 cut(s) 20, 323
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BtsCI GGATG 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 1 cut(s) 352
Csp6I GTAC 3 cut(s) 17, 143, 178
CviAII CATG 2 cut(s) 140, 230
CviJI RGCY 7 cut(s) 131, 152, 217, 287, 293, 344, 387
CviKI_1 RGCY 7 cut(s) 131, 152, 217, 287, 293, 344, 387
CviQI GTAC 3 cut(s) 17, 143, 178
DdeI CTNAG 1 cut(s) 294
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 2 cut(s) 143, 233
FaiI YATR 6 cut(s) 71, 141, 202, 204, 227, 231
FatI CATG 2 cut(s) 139, 229
FauNDI CATATG 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 8
GlaI GCGC 1 cut(s) 351
GluI GCNGC 1 cut(s) 8
HhaI GCGC 1 cut(s) 352
Hin1II CATG 2 cut(s) 143, 233
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 1 cut(s) 38
HpyCH4IV ACGT 2 cut(s) 19, 322
HpyCH4V TGCA 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 2 cut(s) 19, 322
Hsp92II CATG 2 cut(s) 143, 233
HspAI GCGC 1 cut(s) 350
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 1 cut(s) 341
LpnPI CCDG 5 cut(s) 24, 143, 340, 375, 381
LweI GCATC 1 cut(s) 22
MaeII ACGT 2 cut(s) 19, 322
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 293
MflI RGATCY 1 cut(s) 40
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 5 cut(s) 106, 178, 271, 297, 310
Mph1103I ATGCAT 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeI CATATG 1 cut(s) 202
NdeII GATC 1 cut(s) 40
NlaIII CATG 2 cut(s) 143, 233
NlaIV GGNNCC 1 cut(s) 343
NmeAIII GCCGAG 1 cut(s) 340
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 1 cut(s) 143
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 97
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
PpsI GAGTC 1 cut(s) 97
Ppu21I YACGTR 2 cut(s) 20, 323
PsiI TTATAA 1 cut(s) 71
PspCI CACGTG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 3 cut(s) 18, 144, 179
RsaNI GTAC 3 cut(s) 17, 143, 178
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 1 cut(s) 40
SchI GAGTC 1 cut(s) 98
SetI ASST 5 cut(s) 22, 162, 219, 325, 389
SfaNI GCATC 1 cut(s) 22
SmlI CTYRAG 3 cut(s) 36, 146, 218
SmoI CTYRAG 3 cut(s) 36, 146, 218
SsiI CCGC 1 cut(s) 8
StyI CCWWGG 1 cut(s) 288
TaiI ACGT 2 cut(s) 22, 325
TaqI TCGA 1 cut(s) 92
TatI WGTACW 2 cut(s) 142, 177
TauI GCSGC 1 cut(s) 10
TscAI CASTG 1 cut(s) 352
TspDTI ATGAA 3 cut(s) 111, 219, 347
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 1 cut(s) 352
XceI RCATGY 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.