FvH4_5g13250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010318
Physical Location & Seq
Forward (+)
7494087 .. 7496605
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13250.t1

Sequence Viewer

Length: 459 bp
ATGGCGAGTCGGCCGTCTCCGTCGTCTCCACCATCCTCTGCTTCCAACTCCGACCACTCCGCCACTCAACCCACCTCTCTCCCTCCCACGAATCCACACCCACAGGCCGAGGCTGTAATCACCAATGGGGTTCTCAATAATGTCGACGAGAAAAAGCCTGCATCTTTTGATGATCTTCATACCGCAGACCACATCAAGAAGTTCAAGAAGTATGAAGCTGACTACACTCGTTGGTTGACTGCAAAATACTTCTCCAATAAAACTCTTTATGGAGGCAACATATTTGATGGAAATATGACAATACAGGATGAGGTTATAAAGTCAAGCAGGTGGCCTTGTACCAGATCATATGCAGACCCAGTACAGGGCTTTGAAGACCAGACCGGCAGCTGCTCAACTTCTACAACTACAGCTGAAACCTCGCCTAACTTATCAAATGGGAAGCATCCAGTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.58

Weight (kDa)

6.5

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 317
AarI CACCTGC 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 318
AccI GTMKAC 1 cut(s) 144
AciI CCGC 2 cut(s) 60, 183
AcoI YGGCCR 1 cut(s) 11
AfaI GTAC 2 cut(s) 340, 363
AfiI CCNNNNNNNGG 2 cut(s) 364, 365
AgsI TTSAA 2 cut(s) 205, 374
AluBI AGCT 3 cut(s) 218, 390, 413
AluI AGCT 3 cut(s) 218, 390, 413
Alw26I GTCTC 2 cut(s) 21, 30
AoxI GGCC 3 cut(s) 11, 105, 332
ApeKI GCWGC 2 cut(s) 387, 390
AsuHPI GGTGA 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 377, 399
BccI CCATC 2 cut(s) 40, 281
BcoDI GTCTC 2 cut(s) 21, 30
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 318
BisI GCNGC 2 cut(s) 388, 391
BlsI GCNGC 2 cut(s) 389, 392
BmrI ACTGGG 1 cut(s) 353
BmsI GCATC 2 cut(s) 170, 454
BmuI ACTGGG 1 cut(s) 353
BpiI GAAGAC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 365
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 2 cut(s) 359, 449
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 3 cut(s) 32, 313, 445
BseLI CCNNNNNNNGG 2 cut(s) 364, 365
BseNI ACTGG 2 cut(s) 359, 449
BseX3I CGGCCG 1 cut(s) 11
BseXI GCAGC 2 cut(s) 377, 399
Bsh1285I CGRYCG 1 cut(s) 14
BshFI GGCC 3 cut(s) 13, 107, 334
BsiEI CGRYCG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 2 cut(s) 364, 365
BsmAI GTCTC 2 cut(s) 21, 30
BsmBI CGTCTC 2 cut(s) 21, 30
BsnI GGCC 3 cut(s) 13, 107, 334
Bsp143I GATC 2 cut(s) 172, 344
BspACI CCGC 2 cut(s) 60, 183
BspANI GGCC 3 cut(s) 13, 107, 334
BspMI ACCTGC 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 2 cut(s) 359, 449
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 2 cut(s) 172, 344
BstC8I GCNNGC 1 cut(s) 159
BstF5I GGATG 3 cut(s) 32, 313, 445
BstKTI GATC 2 cut(s) 175, 347
BstMAI GTCTC 2 cut(s) 21, 30
BstMBI GATC 2 cut(s) 172, 344
BstMCI CGRYCG 1 cut(s) 14
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 2 cut(s) 377, 399
BstV2I GAAGAC 1 cut(s) 381
BstZI CGGCCG 1 cut(s) 11
BsuRI GGCC 3 cut(s) 13, 107, 334
BtsCI GGATG 3 cut(s) 32, 313, 445
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 383
Csp6I GTAC 2 cut(s) 339, 362
CviJI RGCY 9 cut(s) 13, 107, 113, 157, 218, 334, 369, 390, 413
CviKI_1 RGCY 9 cut(s) 13, 107, 113, 157, 218, 334, 369, 390, 413
CviQI GTAC 2 cut(s) 339, 362
DpnI GATC 2 cut(s) 174, 346
DpnII GATC 2 cut(s) 172, 344
EaeI YGGCCR 1 cut(s) 11
EagI CGGCCG 1 cut(s) 11
EciI GGCGGA 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 11
Esp3I CGTCTC 2 cut(s) 21, 30
FaiI YATR 8 cut(s) 180, 213, 270, 281, 296, 317, 349, 351
FauNDI CATATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 388, 391
FokI GGATG 3 cut(s) 19, 320, 432
Fsp4HI GCNGC 2 cut(s) 388, 391
GluI GCNGC 2 cut(s) 388, 391
HaeIII GGCC 3 cut(s) 13, 107, 334
HapII CCGG 1 cut(s) 384
HincII GTYRAC 2 cut(s) 145, 237
HindII GTYRAC 2 cut(s) 145, 237
HinfI GANTC 2 cut(s) 7, 91
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 145, 237
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 2 cut(s) 196, 205
Hpy8I GTNNAC 2 cut(s) 145, 237
Hpy99I CGWCG 2 cut(s) 25, 149
HpyCH4V TGCA 3 cut(s) 161, 242, 353
Kzo9I GATC 2 cut(s) 172, 344
LpnPI CCDG 9 cut(s) 89, 171, 290, 313, 350, 355, 372, 392, 397
Lsp1109I GCAGC 2 cut(s) 377, 399
LweI GCATC 2 cut(s) 170, 454
MalI GATC 2 cut(s) 174, 346
MboI GATC 2 cut(s) 172, 344
MboII GAAGA 2 cut(s) 167, 386
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 69, 75
MnlI CCTC 7 cut(s) 46, 85, 93, 103, 266, 304, 430
MspA1I CMGCKG 2 cut(s) 390, 413
MspI CCGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 349
NdeII GATC 2 cut(s) 172, 344
NmeAIII GCCGAG 1 cut(s) 133
PaqCI CACCTGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 2 cut(s) 389, 392
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PsiI TTATAA 1 cut(s) 317
PvuII CAGCTG 2 cut(s) 390, 413
RsaI GTAC 2 cut(s) 340, 363
RsaNI GTAC 2 cut(s) 339, 362
SalI GTCGAC 1 cut(s) 143
SatI GCNGC 2 cut(s) 388, 391
Sau3AI GATC 2 cut(s) 172, 344
SchI GAGTC 1 cut(s) 16
SetI ASST 7 cut(s) 77, 220, 315, 332, 392, 415, 422
SfaNI GCATC 2 cut(s) 170, 454
SfcI CTRYAG 1 cut(s) 408
SsiI CCGC 2 cut(s) 60, 183
TaqI TCGA 1 cut(s) 144
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 91
TseI GCWGC 2 cut(s) 387, 390
TspDTI ATGAA 2 cut(s) 167, 228
TspGWI ACGGA 1 cut(s) 9
XmiI GTMKAC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.