FvH4_5g13340

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013673
Physical Location & Seq
Reverse (-)
7517900 .. 7522484
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13340.t1

Sequence Viewer

Length: 1872 bp
ATGCCTACTAAGTTGGAGAGTCCTGTTAAGACGCAGATGGCTGTGGGTCTTGTCAGTGGTCTGCTCAATGGTGAGTGCCGTAAGAGCACAAGAGGAATTTTTGCTCGGAGGAGGCGTGTCTTTGTGCAGACTGAGGCTGGCTGTGTTTTGGGAATGGAGTTGGAAAGGGGTGACAATGCCAACACTGTTAAAAGGAAGTTGCAGCTTGCCCTCAATGTACCTACTGACCATAGCTCTTTGACATTTGGGGATCGAATACTAAATAATGATCTAAGTGCCATCCGCAGTCATTCCCCGCTACTACTTGCAAGGAATGTCATGCATAGGAGCTCCTCAACTCCTTGTCTCTCACCTACTGGGATAAATCTTCAACAGTGGGATCGGAGTGGACCTCTTGAGATTCTAGGGTGTTCAAAGTACTTTGCTAGTATTGATGAATTGATTAAGGACATAGTGGTGGCTATCAAGAAGGGCATTGAACCAATACCTGTTCATAGTGGGCTTGGTGGTGCTTACTACTTCAGGAACATCAGGGGTGAGAGTGTTGCCATTGTGAAACCAACAGATGAGGAGCCTTATGCACCGAACAATCCTAAAGGGTTTGTTGGTACATCTCTCGGGCAGCCAGGACTAAAGCATTCAGTGAGAGTTGGGGAAACAGGGTTTCGAGAAGTTGCTGCCTACCTTTTGGATCTCAATCACTATGCTAATGTGCCTCCGACTGTTCTTGTTAAGGTTACACATTCAATCTTCAATGTTAATTATGGTGTCAATGGTGACAAGATTCAGCAGAAGAAGAAGGTTAGCAAAATTGCATCTCTGCAGCAGTTCATCCCTCATGACTTTGATGCTGGTGACTATGGAAACTCAAGATTTCCTGTAGCTGGTGTTCACAGAATAGGAATTTTAGATATTAGGATCTTTAACACTGACAGGCATGCAGGGAACATTCTGGTTAAAATGCCTGATGGGGAATTTGGTCAGGTGGAGCTCATTCCCATTGATCATGGCCTTTGCTTGCCTGAGCACTTGGAAGATCCCTATTTCGAATGGATTCATTGGCCTCAAGCTTCGGTTCCTTTCTCAGAGGACGAGCTTGAGTATATAAAGAATCTTGATCCAATTCGTGATTCTGAGATGCTCAAGATGGAACTACCCATGATTCGTGAGGCATGTCTTCGAGTTCTGATCCTCTCCACAATATTCCTCCAGGAAGCAGCTGCTTTTGGACTTTGCCTTGCTGAGATAGGTGAAATGATGAGCAGGGAGTTTGGAAGCAAAGAGGAGAAGCCAAGTGAACTTGAGGTTGTATGCCTTGAGACTAGGAGGATAATAGAAGATTTGAACATGTCAAACTTTGTAGCTGAAGAAGACACCGATGCGTTTCTGTCTGATATAGAGTGCGATAAAGCAGGGCTTGATCATCCTCCTCACATCCCAAGTTTCCTCTTCAAGCAGTTTTCATATCCCTTTGGAACTGAAGGTGCTAATAGTCACTACTTGCTTCCTAAGCTAGATGAGAGTCTGGTAGAGGGTAATGTTGACTTGGGTACAAGGGCAGTCACAGCTGAGAGAATCATTAACCAGCCAGATTGGTATAAACCTGTTTCCAAGTTGTCCGTGTCTCTGAAAAAGTTAAATGTGGATGAGACAGGCCAGCTACAGCGGATGGGTACCCTACCTGAAATCAGAAGAAGTGCAAATGACCAGTTACCCACCTCAACAAGCTTTGTGAAACTTGCTGACATGTGCGAGGAGGAATGGGTTCTGTTCCTTGAAAAGTTCAGGGAACTTCTTTACCCCGCCTTCAGCAAACGGAGGTCTACAACCATCGGGCAGAGAAGGAGGCTCGGAACATCATGCACGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

624

Amino Acids

69.74

Weight (kDa)

5.95

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 172 - 415 6.5e-45 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1673
AccB1I GGYRCC 1 cut(s) 1673
AccI GTMKAC 1 cut(s) 1823
AciI CCGC 4 cut(s) 283, 296, 1666, 1803
AclWI GGATC 7 cut(s) 258, 387, 699, 926, 1031, 1112, 1183
AcsI RAATTY 3 cut(s) 96, 903, 974
AcuI CTGAAG 4 cut(s) 505, 1386, 1500, 1792
AfaI GTAC 5 cut(s) 219, 419, 610, 1552, 1675
AfiI CCNNNNNNNGG 1 cut(s) 884
AflIII ACRYGT 2 cut(s) 1347, 1746
AgsI TTSAA 8 cut(s) 371, 414, 479, 747, 754, 1345, 1453, 1778
AjnI CCWGG 2 cut(s) 625, 1209
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
Alw21I GWGCWC 4 cut(s) 89, 332, 993, 1029
Alw26I GTCTC 4 cut(s) 350, 1313, 1629, 1643
AlwI GGATC 7 cut(s) 258, 387, 699, 926, 1031, 1112, 1183
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 3 cut(s) 1009, 1061, 1654
ApeKI GCWGC 6 cut(s) 202, 622, 677, 823, 1217, 1220
ApoI RAATTY 3 cut(s) 96, 903, 974
Asp700I GAANNNNTTC 2 cut(s) 1053, 1764
Asp718I GGTACC 1 cut(s) 1673
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 7 cut(s) 83, 182, 342, 548, 788, 866, 1262
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 389
BanI GGYRCC 1 cut(s) 1673
BanII GRGCYC 2 cut(s) 332, 993
BbsI GAAGAC 2 cut(s) 1169, 1377
Bbv12I GWGCWC 4 cut(s) 89, 332, 993, 1029
BbvI GCAGC 6 cut(s) 214, 634, 664, 835, 1207, 1229
BccI CCATC 6 cut(s) 31, 287, 962, 1141, 1663, 1838
BceAI ACGGC 1 cut(s) 63
BciT130I CCWGG 2 cut(s) 627, 1211
BclI TGATCA 2 cut(s) 1003, 1420
BcoDI GTCTC 4 cut(s) 350, 1313, 1629, 1643
BfaI CTAG 4 cut(s) 404, 426, 1323, 1514
BfmI CTRYAG 3 cut(s) 821, 879, 1661
BisI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
BlsI GCNGC 6 cut(s) 204, 624, 679, 825, 1219, 1222
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 2 cut(s) 627, 1211
Bme18I GGWCC 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 3 cut(s) 573, 1077, 1675
BmrFI CCNGG 2 cut(s) 627, 1211
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 4 cut(s) 824, 838, 1128, 1369
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 2 cut(s) 1169, 1377
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpmI CTGGAG 1 cut(s) 1193
Bpu10I CCTNAGC 2 cut(s) 1023, 1509
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 7 cut(s) 416, 853, 1050, 1118, 1127, 1322, 1337
BsaBI GATNNNNATC 1 cut(s) 696
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 1 cut(s) 884
Bse1I ACTGG 2 cut(s) 361, 1708
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 2 cut(s) 627, 1211
BseGI GGATG 6 cut(s) 279, 831, 1423, 1434, 1651, 1674
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 1 cut(s) 884
BseMII CTCAG 6 cut(s) 123, 1014, 1098, 1125, 1233, 1560
BseNI ACTGG 2 cut(s) 361, 1708
BseRI GAGGAG 6 cut(s) 124, 322, 584, 1298, 1419, 1769
BseXI GCAGC 6 cut(s) 214, 634, 664, 835, 1207, 1229
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 3 cut(s) 1011, 1063, 1656
BshNI GGYRCC 1 cut(s) 1673
BsiHKAI GWGCWC 4 cut(s) 89, 332, 993, 1029
BsiHKCI CYCGRG 1 cut(s) 617
BslI CCNNNNNNNGG 1 cut(s) 884
BsmAI GTCTC 4 cut(s) 350, 1313, 1629, 1643
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 3 cut(s) 1011, 1063, 1656
BsoBI CYCGRG 1 cut(s) 617
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 4 cut(s) 89, 332, 993, 1029
BspACI CCGC 4 cut(s) 283, 296, 1666, 1803
BspANI GGCC 3 cut(s) 1011, 1063, 1656
BspCNI CTCAG 6 cut(s) 124, 1015, 1097, 1126, 1234, 1561
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 573, 1077, 1675
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 7 cut(s) 258, 387, 699, 926, 1031, 1112, 1183
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 1673
BsrI ACTGG 2 cut(s) 361, 1708
Bst2UI CCWGG 2 cut(s) 627, 1211
Bst4CI ACNGT 3 cut(s) 187, 375, 724
Bst6I CTCTTC 1 cut(s) 1454
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 5 cut(s) 139, 207, 939, 1019, 1658
BstDEI CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1134, 1242, 1509, 1569
BstF5I GGATG 6 cut(s) 279, 831, 1423, 1434, 1651, 1674
BstMAI GTCTC 4 cut(s) 350, 1313, 1629, 1643
BstMWI GCNNNNNNNGC 3 cut(s) 84, 1510, 1565
BstNI CCWGG 2 cut(s) 627, 1211
BstNSI RCATGY 4 cut(s) 941, 1176, 1351, 1750
BstSCI CCNGG 2 cut(s) 625, 1209
BstSFI CTRYAG 3 cut(s) 821, 879, 1661
BstV1I GCAGC 6 cut(s) 214, 634, 664, 835, 1207, 1229
BstV2I GAAGAC 2 cut(s) 1169, 1377
BstX2I RGATCY 3 cut(s) 691, 918, 1036
BstYI RGATCY 3 cut(s) 691, 918, 1036
BsuRI GGCC 3 cut(s) 1011, 1063, 1656
BtsCI GGATG 6 cut(s) 279, 831, 1423, 1434, 1651, 1674
BtsIMutI CAGTG 5 cut(s) 61, 183, 380, 648, 927
Cac8I GCNNGC 5 cut(s) 139, 207, 939, 1019, 1658
CciI TCATGA 1 cut(s) 838
Cfr13I GGNCC 1 cut(s) 389
CseI GACGC 1 cut(s) 40
Csp6I GTAC 5 cut(s) 218, 418, 609, 1551, 1674
CviAII CATG 9 cut(s) 319, 839, 938, 1007, 1159, 1173, 1348, 1747, 1860
CviQI GTAC 5 cut(s) 218, 418, 609, 1551, 1674
DdeI CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1134, 1242, 1509, 1569
Eam1104I CTCTTC 1 cut(s) 1454
EarI CTCTTC 1 cut(s) 1454
Ecl136II GAGCTC 2 cut(s) 330, 991
Eco24I GRGCYC 2 cut(s) 332, 993
Eco47I GGWCC 1 cut(s) 389
Eco53kI GAGCTC 2 cut(s) 330, 991
Eco57I CTGAAG 4 cut(s) 505, 1386, 1500, 1792
Eco88I CYCGRG 1 cut(s) 617
EcoICRI GAGCTC 2 cut(s) 330, 991
EcoRII CCWGG 2 cut(s) 625, 1209
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 332, 993
FaeI CATG 9 cut(s) 322, 842, 941, 1010, 1162, 1176, 1351, 1750, 1863
FalI AAGNNNNNCTT 2 cut(s) 1401, 1433
FatI CATG 9 cut(s) 318, 838, 937, 1006, 1158, 1172, 1347, 1746, 1859
FauI CCCGC 2 cut(s) 303, 1810
FbaI TGATCA 2 cut(s) 1003, 1420
FblI GTMKAC 1 cut(s) 1823
Fnu4HI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
FokI GGATG 6 cut(s) 266, 818, 1410, 1421, 1658, 1681
FriOI GRGCYC 2 cut(s) 332, 993
Fsp4HI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
FspBI CTAG 4 cut(s) 404, 426, 1323, 1514
GluI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
GsuI CTGGAG 1 cut(s) 1193
HaeIII GGCC 3 cut(s) 1011, 1063, 1656
HgaI GACGC 1 cut(s) 40
Hin1II CATG 9 cut(s) 322, 842, 941, 1010, 1162, 1176, 1351, 1750, 1863
HincII GTYRAC 1 cut(s) 1543
HindII GTYRAC 1 cut(s) 1543
HindIII AAGCTT 2 cut(s) 1068, 1726
HinfI GANTC 9 cut(s) 19, 400, 784, 1054, 1111, 1130, 1162, 1522, 1575
HphI GGTGA 7 cut(s) 83, 182, 342, 548, 788, 866, 1262
Hpy166II GTNNAC 5 cut(s) 389, 892, 1298, 1543, 1824
Hpy8I GTNNAC 5 cut(s) 389, 892, 1298, 1543, 1824
HpyAV CCTTC 5 cut(s) 463, 793, 1475, 1816, 1836
HpyCH4III ACNGT 3 cut(s) 187, 375, 724
HpyCH4IV ACGT 1 cut(s) 1865
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 1510, 1565
HpyF3I CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1134, 1242, 1509, 1569
HpySE526I ACGT 1 cut(s) 1865
Hsp92II CATG 9 cut(s) 322, 842, 941, 1010, 1162, 1176, 1351, 1750, 1863
KpnI GGTACC 1 cut(s) 1677
Ksp22I TGATCA 2 cut(s) 1003, 1420
LmnI GCTCC 4 cut(s) 327, 335, 571, 988
Lsp1109I GCAGC 6 cut(s) 214, 634, 664, 835, 1207, 1229
LweI GCATC 4 cut(s) 824, 838, 1128, 1369
MaeI CTAG 4 cut(s) 404, 426, 1323, 1514
MaeII ACGT 1 cut(s) 1865
MaeIII GTNAC 7 cut(s) 170, 736, 776, 854, 1493, 1561, 1710
MflI RGATCY 3 cut(s) 691, 918, 1036
MhlI GDGCHC 4 cut(s) 89, 332, 993, 1029
MluCI AATT 7 cut(s) 96, 437, 760, 810, 903, 974, 1122
MlyI GAGTC 2 cut(s) 28, 1531
MmeI TCCRAC 2 cut(s) 141, 743
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 2 cut(s) 1053, 1764
MseI TTAA 9 cut(s) 27, 189, 444, 732, 759, 924, 957, 1581, 1637
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 3 cut(s) 1220, 1568, 1666
MspR9I CCNGG 2 cut(s) 627, 1211
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 2 cut(s) 627, 1211
MwoI GCNNNNNNNGC 3 cut(s) 84, 1510, 1565
NlaIII CATG 9 cut(s) 322, 842, 941, 1010, 1162, 1176, 1351, 1750, 1863
NlaIV GGNNCC 3 cut(s) 573, 1077, 1675
NmuCI GTSAC 5 cut(s) 170, 776, 854, 1493, 1561
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 4 cut(s) 941, 1176, 1351, 1750
NspV TTCGAA 1 cut(s) 1047
PaeI GCATGC 1 cut(s) 941
PagI TCATGA 1 cut(s) 838
PciI ACATGT 2 cut(s) 1347, 1746
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 2 cut(s) 1053, 1764
PfeI GAWTC 7 cut(s) 400, 784, 1054, 1111, 1130, 1162, 1575
PfoI TCCNGGA 1 cut(s) 1209
PkrI GCNGC 6 cut(s) 204, 624, 679, 825, 1219, 1222
PleI GAGTC 2 cut(s) 27, 1530
PpsI GAGTC 2 cut(s) 27, 1530
PscI ACATGT 2 cut(s) 1347, 1746
Psp124BI GAGCTC 2 cut(s) 332, 993
Psp6I CCWGG 2 cut(s) 625, 1209
PspGI CCWGG 2 cut(s) 625, 1209
PspN4I GGNNCC 3 cut(s) 573, 1077, 1675
PspPI GGNCC 1 cut(s) 389
PsrI GAACNNNNNNTAC 2 cut(s) 873, 905
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 3 cut(s) 691, 918, 1036
PvuII CAGCTG 2 cut(s) 1220, 1568
RsaI GTAC 5 cut(s) 219, 419, 610, 1552, 1675
RsaNI GTAC 5 cut(s) 218, 418, 609, 1551, 1674
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 332, 993
SaqAI TTAA 9 cut(s) 27, 189, 444, 732, 759, 924, 957, 1581, 1637
SatI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
Sau96I GGNCC 1 cut(s) 389
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 2 cut(s) 28, 1531
ScrFI CCNGG 2 cut(s) 627, 1211
SduI GDGCHC 4 cut(s) 89, 332, 993, 1029
SfaNI GCATC 4 cut(s) 824, 838, 1128, 1369
SfcI CTRYAG 3 cut(s) 821, 879, 1661
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 7 cut(s) 395, 868, 1065, 1097, 1142, 1301, 1316
SmoI CTYRAG 7 cut(s) 395, 868, 1065, 1097, 1142, 1301, 1316
SphI GCATGC 1 cut(s) 941
Sse9I AATT 7 cut(s) 96, 437, 760, 810, 903, 974, 1122
SsiI CCGC 4 cut(s) 283, 296, 1666, 1803
SspI AATATT 1 cut(s) 1203
SspMI CTAG 4 cut(s) 404, 426, 1323, 1514
SstI GAGCTC 2 cut(s) 332, 993
StyD4I CCNGG 2 cut(s) 625, 1209
TaaI ACNGT 3 cut(s) 187, 375, 724
TaiI ACGT 1 cut(s) 1868
TaqI TCGA 4 cut(s) 253, 667, 1047, 1180
TasI AATT 7 cut(s) 96, 437, 760, 810, 903, 974, 1122
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 7 cut(s) 400, 784, 1054, 1111, 1130, 1162, 1575
Tru1I TTAA 9 cut(s) 27, 189, 444, 732, 759, 924, 957, 1581, 1637
Tru9I TTAA 9 cut(s) 27, 189, 444, 732, 759, 924, 957, 1581, 1637
TscAI CASTG 5 cut(s) 61, 190, 380, 648, 934
TseFI GTSAC 5 cut(s) 170, 776, 854, 1493, 1561
TseI GCWGC 6 cut(s) 202, 622, 677, 823, 1217, 1220
Tsp45I GTSAC 5 cut(s) 170, 776, 854, 1493, 1561
TspDTI ATGAA 5 cut(s) 450, 482, 820, 1046, 1452
TspGWI ACGGA 2 cut(s) 1609, 1831
TspRI CASTG 5 cut(s) 61, 190, 380, 648, 934
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 3 cut(s) 96, 903, 974
XceI RCATGY 4 cut(s) 941, 1176, 1351, 1750
XmiI GTMKAC 1 cut(s) 1823
XmnI GAANNNNTTC 2 cut(s) 1053, 1764
XspI CTAG 4 cut(s) 404, 426, 1323, 1514
ZrmI AGTACT 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.