RLG00000005022

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013673
Physical Location & Seq
Reverse (-)
64386497 .. 64389593
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005022

Sequence Viewer

Length: 2109 bp
ATGTTGATTCTTCTCCGTCATCGCCTCCTATCCTCCGACTCTGTTCTCTATTCCTCTCTCTCTCGCGATGATCTTGCTCTCCAAGATTTAACGTCCGGGTTTGATCTAGTAGCGTCAAACAGAAGATTTCCTGATGTAGTGACCCGTTGGCACCTGCTGAGAGAGCGGAAGGATCTGCATCCTGTTGTCACCTTGATCCCCTGCTTGAAAGTAACATCTTCATCCTTAGTTATGCCTACTAAGTTGGAGAGTCCCGTTAAGACGCAGATGGCTGTGGGACTTGTCAGTGGTCTGCTCAATGGTGAGTGCCGTGAGAGCACAAGAGGAATTCTTGCTCGGAGGAGGCGTGTCTTTGTGCAGACTGAGACTGGCTGCGTTTTGGGAATGGACTTGGAAAGGGGTGACAATGCCAACACAGTTAAAAGGAGGTTGCAGCTTGCCCTCAATGTACCTACTGACCAGAGCTCTTTGACATTCGGGGATCGAATTTTGAATAATGATCTAAGTGCCGTCCGCAATCATTCCCCGCTACTACTTGCAAGGAATGTCATGCATAGAAGCTCCTCAACTCCTTGTCTCTCACCTACTGGGAGAAATCTTCAACATTGGGATCGGAGTGGTCCTATTGAGATTCTTGGGTGTTCAAAATACTTTGCTAGTATTGATGAACTGATTAAGGACATAGTGGTGGCTATCAAGAAGGGCATTGAACCAATACCTGTTCATAGTGGGCTTGGTGGTGCTTACTACTTCAGGAACATCAGGGGTGAGAGTGTTGCCATTGTGAAACCAACAGATGAGGAGCCATATGCACCCAACAACCCCAAAGGCTTTGTAGGCACATCTCTTGGGCTGCCAGGCCTGAAGCATTCAGTGAGAGTTGGGGAAACAGGGTTTCGAGAAGTTGCTGCCTACCTTTTGGATGTCAATCACTATGCTAATGTGCCTCCAACTGTTCTTGTTAAGGTGACACATTCAATCTTCAATGTTAATTATGGTGTCAATGGAGACAAGATTCAGCAGAAGAAAAAGGTTAGTAAAATTGCATCTCTGCAGCAGTTCATCTGTCATGACTTTGATGCTGGTGATTATGGAACCTCAAGTTTTCCTGTAGCTGGTGTTCACAGAATAGGCATTTTAGATATTAGGACCTTTAACACTGACAGGCATGCAGGAAACATTCTGGTTAAAATGCCCGATGGGGAGTTTGGTCAGGTGGAGCTCATTCCCATAGACCATGGCCTTTGCTTGCCTGAGCACTTGGAAGATCCCTATTTTGAATGGATTCATTGGCCTCAAGCTTCAATTCCTTTCTCAGAGGACGAGCTTGAGTATATAAAGAATCTTGATCCAATTCGTGATTCCGAGATGCTCAAGATGGAACTGCCCATGATTCGTGAGGCATGTCTTCGAGTTCTGGTCCTCTCCACAATTTTCCTCCAGGAAGCAGCTGCTTTTGGACTTTGCCTTGCTGAAATAGGTGAAATGATGAGCAGGGAGTTTGGAAGCAAAGAGGAGAAGCCAAGTGAACTTGAGGTTGTATGCCTTGAGACCAGGAGGATCATAGCAGATTCGAACATGTCAAACTTTGTGGCTGAAGAAGACAACAATCAGTGTCTGTCTGATACAGAGTGCGATAAAGCAGGGCTTGATCCTTCTCCTCAGATCCCATGTTTCCTCTTCAAGCAGTTTTCATATCCCTTTGGACCTGATGGTGCTAATGGTCACTACTTACTTCCTAAGCTAGATGAGAGTTTGGAAGAGGATATTGTTGACTTGGATACAAGGGCAATCAGAGCTGAGGGAATCATTACCCAGCCAAATTGGTATAAAACTGAAACTGTTTCGAAGTTGTCCATGTCTCTGAAAAAGTTAAATGTGGATGAGACAAGCCAGCTACAGCGGAAGGGTACCTTACCTGGAATCAGAAGAAGTGCAAATGACCAGTTACCCGCCTCAACAAGCTTTGTGAAACTTGCTGATATGTGTGAGGAGGAATGGGTTCTGTTCCTTGAAAAGTTTAGGGAACTTCTTTACCCCGCCCTTGGCAAACGAAGGTCTACCACCATTGGGCAGAGAAGGAGGCTCGGAACATCATGCACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

703

Amino Acids

78.52

Weight (kDa)

6.07

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 249 - 492 2.3e-43 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 162
Acc65I GGTACC 1 cut(s) 1910
AccB1I GGYRCC 2 cut(s) 150, 1910
AccBSI CCGCTC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 2060
AccII CGCG 1 cut(s) 66
AciI CCGC 6 cut(s) 166, 514, 527, 1903, 1953, 2040
AclWI GGATC 9 cut(s) 180, 190, 489, 618, 1262, 1343, 1568, 1646, 1660
AcsI RAATTY 2 cut(s) 327, 486
AcuI CTGAAG 3 cut(s) 736, 884, 1617
AfaI GTAC 2 cut(s) 450, 1912
AfiI CCNNNNNNNGG 3 cut(s) 1115, 2045, 2070
AflIII ACRYGT 1 cut(s) 1578
AjnI CCWGG 4 cut(s) 856, 1440, 1553, 1918
AjuI GAANNNNNNNTTGG 2 cut(s) 1345, 1377
Alw21I GWGCWC 4 cut(s) 320, 467, 1224, 1260
Alw26I GTCTC 6 cut(s) 359, 581, 1002, 1544, 1866, 1880
AlwI GGATC 9 cut(s) 180, 190, 489, 618, 1262, 1343, 1568, 1646, 1660
AlwNI CAGNNNCTG 1 cut(s) 1618
AoxI GGCC 3 cut(s) 859, 1240, 1292
ApeKI GCWGC 7 cut(s) 372, 433, 853, 908, 1054, 1448, 1451
ApoI RAATTY 2 cut(s) 327, 486
Asp700I GAANNNNTTC 2 cut(s) 1284, 2001
Asp718I GGTACC 1 cut(s) 1910
AspS9I GGNCC 4 cut(s) 620, 1149, 1420, 1706
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 8 cut(s) 181, 314, 413, 573, 779, 979, 1097, 1493
AsuII TTCGAA 2 cut(s) 1574, 1847
AvaII GGWCC 4 cut(s) 620, 1149, 1420, 1706
BanI GGYRCC 2 cut(s) 150, 1910
BanII GRGCYC 2 cut(s) 467, 1224
BbsI GAAGAC 2 cut(s) 1400, 1608
Bbv12I GWGCWC 4 cut(s) 320, 467, 1224, 1260
BbvCI CCTCAGC 1 cut(s) 1800
BbvI GCAGC 7 cut(s) 359, 445, 840, 895, 1066, 1438, 1460
BccI CCATC 4 cut(s) 262, 1193, 1372, 1706
BceAI ACGGC 2 cut(s) 294, 494
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 4 cut(s) 858, 1442, 1555, 1920
BciVI GTATCC 1 cut(s) 1774
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 6 cut(s) 359, 581, 1002, 1544, 1866, 1880
BfaI CTAG 3 cut(s) 107, 657, 1745
BfmI CTRYAG 3 cut(s) 1052, 1110, 1898
BfuAI ACCTGC 1 cut(s) 162
BfuI GTATCC 1 cut(s) 1774
BisI GCNGC 7 cut(s) 373, 434, 854, 909, 1055, 1449, 1452
BlsI GCNGC 7 cut(s) 374, 435, 855, 910, 1056, 1450, 1453
Bme1390I CCNGG 5 cut(s) 97, 858, 1442, 1555, 1920
Bme18I GGWCC 4 cut(s) 620, 1149, 1420, 1706
BmgT120I GGNCC 4 cut(s) 620, 1149, 1420, 1706
BmiI GGNNCC 4 cut(s) 152, 804, 1096, 1912
BmrFI CCNGG 5 cut(s) 97, 858, 1442, 1555, 1920
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 4 cut(s) 187, 1055, 1069, 1359
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 2 cut(s) 1400, 1608
BpmI CTGGAG 1 cut(s) 1424
Bpu10I CCTNAGC 3 cut(s) 1254, 1740, 1800
Bpu14I TTCGAA 2 cut(s) 1574, 1847
BpuEI CTTGAG 6 cut(s) 1084, 1281, 1349, 1358, 1553, 1568
BpuMI CCSGG 1 cut(s) 97
BsaBI GATNNNNATC 2 cut(s) 177, 927
BsaI GGTCTC 1 cut(s) 1544
BsaJI CCNNGG 2 cut(s) 1237, 2044
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 1115, 2045, 2070
Bse1I ACTGG 3 cut(s) 373, 592, 1945
Bse8I GATNNNNATC 2 cut(s) 177, 927
BseBI CCWGG 4 cut(s) 858, 1442, 1555, 1920
BseDI CCNNGG 2 cut(s) 1237, 2044
BseGI GGATG 4 cut(s) 178, 221, 928, 1888
BseJI GATNNNNATC 2 cut(s) 177, 927
BseLI CCNNNNNNNGG 3 cut(s) 1115, 2045, 2070
BseMII CTCAG 6 cut(s) 149, 354, 1245, 1329, 1676, 1791
BseNI ACTGG 3 cut(s) 373, 592, 1945
BseRI GAGGAG 6 cut(s) 355, 553, 815, 1529, 1650, 2006
BseXI GCAGC 7 cut(s) 359, 445, 840, 895, 1066, 1438, 1460
BseYI CCCAGC 1 cut(s) 1815
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 861, 1242, 1294
BshNI GGYRCC 2 cut(s) 150, 1910
BsiHKAI GWGCWC 4 cut(s) 320, 467, 1224, 1260
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 237, 291
BslI CCNNNNNNNGG 3 cut(s) 1115, 2045, 2070
BsmAI GTCTC 6 cut(s) 359, 581, 1002, 1544, 1866, 1880
BsmFI GGGAC 2 cut(s) 237, 291
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 3 cut(s) 861, 1242, 1294
Bso31I GGTCTC 1 cut(s) 1544
Bsp119I TTCGAA 2 cut(s) 1574, 1847
Bsp1286I GDGCHC 4 cut(s) 320, 467, 1224, 1260
Bsp19I CCATGG 1 cut(s) 1237
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 6 cut(s) 166, 514, 527, 1903, 1953, 2040
BspANI GGCC 3 cut(s) 861, 1242, 1294
BspCNI CTCAG 6 cut(s) 150, 355, 1246, 1328, 1675, 1792
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 1069
BspLI GGNNCC 4 cut(s) 152, 804, 1096, 1912
BspMAI CTGCAG 1 cut(s) 1056
BspMI ACCTGC 1 cut(s) 162
BspPI GGATC 9 cut(s) 180, 190, 489, 618, 1262, 1343, 1568, 1646, 1660
BspT104I TTCGAA 2 cut(s) 1574, 1847
BspT107I GGYRCC 2 cut(s) 150, 1910
BspTNI GGTCTC 1 cut(s) 1544
BsrBI CCGCTC 1 cut(s) 166
BsrI ACTGG 3 cut(s) 373, 592, 1945
BssECI CCNNGG 2 cut(s) 1237, 2044
BssT1I CCWWGG 2 cut(s) 1237, 2044
Bst2UI CCWGG 4 cut(s) 858, 1442, 1555, 1920
Bst4CI ACNGT 3 cut(s) 418, 955, 1843
Bst6I CTCTTC 2 cut(s) 1685, 1755
BstBI TTCGAA 2 cut(s) 1574, 1847
BstC8I GCNNGC 4 cut(s) 438, 1170, 1250, 1895
BstDSI CCRYGG 1 cut(s) 1237
BstF5I GGATG 4 cut(s) 178, 221, 928, 1888
BstFNI CGCG 1 cut(s) 66
BstMAI GTCTC 6 cut(s) 359, 581, 1002, 1544, 1866, 1880
BstMWI GCNNNNNNNGC 4 cut(s) 163, 315, 837, 1796
BstNI CCWGG 4 cut(s) 858, 1442, 1555, 1920
BstNSI RCATGY 3 cut(s) 1172, 1407, 1582
BstSCI CCNGG 5 cut(s) 95, 856, 1440, 1553, 1918
BstSFI CTRYAG 3 cut(s) 1052, 1110, 1898
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 7 cut(s) 359, 445, 840, 895, 1066, 1438, 1460
BstV2I GAAGAC 2 cut(s) 1400, 1608
BstX2I RGATCY 3 cut(s) 172, 1267, 1665
BstYI RGATCY 3 cut(s) 172, 1267, 1665
BsuI GTATCC 1 cut(s) 1774
BsuRI GGCC 3 cut(s) 861, 1242, 1294
BtgI CCRYGG 1 cut(s) 1237
BtgZI GCGATG 2 cut(s) 5, 81
BtsCI GGATG 4 cut(s) 178, 221, 928, 1888
BtsIMutI CAGTG 4 cut(s) 292, 879, 1158, 1619
BtuMI TCGCGA 1 cut(s) 66
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 4 cut(s) 438, 1170, 1250, 1895
CaiI CAGNNNCTG 1 cut(s) 1618
CciI TCATGA 1 cut(s) 1069
Cfr13I GGNCC 4 cut(s) 620, 1149, 1420, 1706
CseI GACGC 2 cut(s) 102, 271
Csp6I GTAC 2 cut(s) 449, 1911
CspCI CAANNNNNGTGG 2 cut(s) 1572, 1607
CviQI GTAC 2 cut(s) 449, 1911
Eam1104I CTCTTC 2 cut(s) 1685, 1755
EarI CTCTTC 2 cut(s) 1685, 1755
Ecl136II GAGCTC 2 cut(s) 465, 1222
Eco130I CCWWGG 2 cut(s) 1237, 2044
Eco147I AGGCCT 1 cut(s) 861
Eco24I GRGCYC 2 cut(s) 467, 1224
Eco31I GGTCTC 1 cut(s) 1544
Eco47I GGWCC 4 cut(s) 620, 1149, 1420, 1706
Eco53kI GAGCTC 2 cut(s) 465, 1222
Eco57I CTGAAG 3 cut(s) 736, 884, 1617
EcoICRI GAGCTC 2 cut(s) 465, 1222
EcoO109I RGGNCCY 1 cut(s) 1149
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 4 cut(s) 856, 1440, 1553, 1918
EcoT14I CCWWGG 2 cut(s) 1237, 2044
EcoT22I ATGCAT 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 467, 1224
ErhI CCWWGG 2 cut(s) 1237, 2044
FalI AAGNNNNNCTT 4 cut(s) 1632, 1664, 1898, 1930
FaqI GGGAC 2 cut(s) 237, 291
FauI CCCGC 3 cut(s) 534, 1960, 2047
FauNDI CATATG 1 cut(s) 808
FblI GTMKAC 1 cut(s) 2060
Fnu4HI GCNGC 7 cut(s) 373, 434, 854, 909, 1055, 1449, 1452
FokI GGATG 4 cut(s) 165, 208, 935, 1895
FriOI GRGCYC 2 cut(s) 467, 1224
Fsp4HI GCNGC 7 cut(s) 373, 434, 854, 909, 1055, 1449, 1452
FspBI CTAG 3 cut(s) 107, 657, 1745
GluI GCNGC 7 cut(s) 373, 434, 854, 909, 1055, 1449, 1452
GsaI CCCAGC 1 cut(s) 1819
GsuI CTGGAG 1 cut(s) 1424
HaeIII GGCC 3 cut(s) 861, 1242, 1294
HapII CCGG 1 cut(s) 96
HgaI GACGC 2 cut(s) 102, 271
HincII GTYRAC 1 cut(s) 1774
HindII GTYRAC 1 cut(s) 1774
HindIII AAGCTT 2 cut(s) 1299, 1963
HpaII CCGG 1 cut(s) 96
HphI GGTGA 8 cut(s) 181, 314, 413, 573, 779, 979, 1097, 1493
Hpy166II GTNNAC 4 cut(s) 1123, 1529, 1774, 2061
Hpy8I GTNNAC 4 cut(s) 1123, 1529, 1774, 2061
HpyAV CCTTC 6 cut(s) 163, 694, 1665, 1900, 2049, 2073
HpyCH4III ACNGT 3 cut(s) 418, 955, 1843
HpyCH4IV ACGT 1 cut(s) 92
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 315, 837, 1796
HpySE526I ACGT 1 cut(s) 92
KpnI GGTACC 1 cut(s) 1914
LmnI GCTCC 3 cut(s) 566, 802, 1219
Lsp1109I GCAGC 7 cut(s) 359, 445, 840, 895, 1066, 1438, 1460
LweI GCATC 4 cut(s) 187, 1055, 1069, 1359
MaeI CTAG 3 cut(s) 107, 657, 1745
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 7 cut(s) 139, 187, 211, 401, 967, 1724, 1947
MbiI CCGCTC 1 cut(s) 166
MflI RGATCY 3 cut(s) 172, 1267, 1665
MhlI GDGCHC 4 cut(s) 320, 467, 1224, 1260
MluCI AATT 8 cut(s) 327, 486, 991, 1041, 1305, 1353, 1431, 1822
MlyI GAGTC 2 cut(s) 32, 259
MmeI TCCRAC 3 cut(s) 60, 225, 974
Mph1103I ATGCAT 1 cut(s) 555
MroXI GAANNNNTTC 2 cut(s) 1284, 2001
MseI TTAA 9 cut(s) 89, 258, 420, 675, 963, 990, 1155, 1188, 1874
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 1451, 1903
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 5 cut(s) 97, 858, 1442, 1555, 1920
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 4 cut(s) 858, 1442, 1555, 1920
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 4 cut(s) 163, 315, 837, 1796
NciI CCSGG 1 cut(s) 97
NcoI CCATGG 1 cut(s) 1237
NdeI CATATG 1 cut(s) 808
NlaIV GGNNCC 4 cut(s) 152, 804, 1096, 1912
NmuCI GTSAC 5 cut(s) 139, 187, 401, 967, 1724
NruI TCGCGA 1 cut(s) 66
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 3 cut(s) 1172, 1407, 1582
NspV TTCGAA 2 cut(s) 1574, 1847
PaeI GCATGC 1 cut(s) 1172
PagI TCATGA 1 cut(s) 1069
PaqCI CACCTGC 1 cut(s) 162
PceI AGGCCT 1 cut(s) 861
PciI ACATGT 1 cut(s) 1578
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 868
PdmI GAANNNNTTC 2 cut(s) 1284, 2001
PfoI TCCNGGA 1 cut(s) 1440
PkrI GCNGC 7 cut(s) 374, 435, 855, 910, 1056, 1450, 1453
PleI GAGTC 2 cut(s) 32, 258
PpsI GAGTC 2 cut(s) 32, 258
PpuMI RGGWCCY 1 cut(s) 1149
PscI ACATGT 1 cut(s) 1578
Psp124BI GAGCTC 2 cut(s) 467, 1224
Psp5II RGGWCCY 1 cut(s) 1149
Psp6I CCWGG 4 cut(s) 856, 1440, 1553, 1918
PspFI CCCAGC 1 cut(s) 1815
PspGI CCWGG 4 cut(s) 856, 1440, 1553, 1918
PspN4I GGNNCC 4 cut(s) 152, 804, 1096, 1912
PspPI GGNCC 4 cut(s) 620, 1149, 1420, 1706
PspPPI RGGWCCY 1 cut(s) 1149
PsrI GAACNNNNNNTAC 2 cut(s) 1104, 1136
PstI CTGCAG 1 cut(s) 1056
PstNI CAGNNNCTG 1 cut(s) 1618
PsuI RGATCY 3 cut(s) 172, 1267, 1665
PvuII CAGCTG 1 cut(s) 1451
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 2 cut(s) 450, 1912
RsaNI GTAC 2 cut(s) 449, 1911
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 2 cut(s) 467, 1224
SaqAI TTAA 9 cut(s) 89, 258, 420, 675, 963, 990, 1155, 1188, 1874
SatI GCNGC 7 cut(s) 373, 434, 854, 909, 1055, 1449, 1452
Sau96I GGNCC 4 cut(s) 620, 1149, 1420, 1706
SchI GAGTC 2 cut(s) 32, 259
ScrFI CCNGG 5 cut(s) 97, 858, 1442, 1555, 1920
SduI GDGCHC 4 cut(s) 320, 467, 1224, 1260
SfaNI GCATC 4 cut(s) 187, 1055, 1069, 1359
SfcI CTRYAG 3 cut(s) 1052, 1110, 1898
SfuI TTCGAA 2 cut(s) 1574, 1847
SinI GGWCC 4 cut(s) 620, 1149, 1420, 1706
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 6 cut(s) 1099, 1296, 1328, 1373, 1532, 1547
SmoI CTYRAG 6 cut(s) 1099, 1296, 1328, 1373, 1532, 1547
SphI GCATGC 1 cut(s) 1172
Sse9I AATT 8 cut(s) 327, 486, 991, 1041, 1305, 1353, 1431, 1822
SseBI AGGCCT 1 cut(s) 861
SsiI CCGC 6 cut(s) 166, 514, 527, 1903, 1953, 2040
SspMI CTAG 3 cut(s) 107, 657, 1745
SstI GAGCTC 2 cut(s) 467, 1224
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 5 cut(s) 95, 856, 1440, 1553, 1918
StyI CCWWGG 2 cut(s) 1237, 2044
TaaI ACNGT 3 cut(s) 418, 955, 1843
TaiI ACGT 1 cut(s) 95
TaqI TCGA 5 cut(s) 484, 898, 1411, 1574, 1847
TasI AATT 8 cut(s) 327, 486, 991, 1041, 1305, 1353, 1431, 1822
Tru1I TTAA 9 cut(s) 89, 258, 420, 675, 963, 990, 1155, 1188, 1874
Tru9I TTAA 9 cut(s) 89, 258, 420, 675, 963, 990, 1155, 1188, 1874
TscAI CASTG 4 cut(s) 292, 879, 1165, 1619
TseFI GTSAC 5 cut(s) 139, 187, 401, 967, 1724
TseI GCWGC 7 cut(s) 372, 433, 853, 908, 1054, 1448, 1451
Tsp45I GTSAC 5 cut(s) 139, 187, 401, 967, 1724
TspDTI ATGAA 6 cut(s) 210, 681, 713, 1051, 1277, 1683
TspGWI ACGGA 1 cut(s) 5
TspRI CASTG 4 cut(s) 292, 879, 1165, 1619
VpaK11BI GGWCC 4 cut(s) 620, 1149, 1420, 1706
XapI RAATTY 2 cut(s) 327, 486
XceI RCATGY 3 cut(s) 1172, 1407, 1582
XmiI GTMKAC 1 cut(s) 2060
XmnI GAANNNNTTC 2 cut(s) 1284, 2001
XspI CTAG 3 cut(s) 107, 657, 1745
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.