Prupe.5G210600_v2.0.a1

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013673
Physical Location & Seq
Forward (+)
16746945 .. 16750224
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G210600.2

Sequence Viewer

Length: 1770 bp
ATGACTACAAAGTTGGAGAGTCCTGTTAAGACCCAGATGGTTGTGACACTCATCAGTGGGCTGCTCAATCGGGATTCCCATGAGAGCACAAGAGGAAACCTTGCCCGGAGGAGACGTGTCTTTGTGCAGACTGAGACTGGCTGTGTCTTGGGAATGGAGTTGGAAAGGGGTGACAATGCTCACACAGTCAAAAGGAGGTTGCAGCTTGCCCTCAGTGTACCTACCGACCAGAGCTCTTTGACATTTGGGGATCGTATATTAAACAATGATCTTAGTGCCATCCGCAATCATTCCCCACTGCTACTTGCAAGAAATGCCATGCATAGAAGCTCATCGACTCCTTGTCTCTCACCTACGGGAAAGAATCTTCTCCAGAAGGATCAAAGTGATCCTATTGAGATTTTAGGGTGTCCAAATAACTTTGCCAGAATTGATGAACTGGTTAAGGACATTGTGGCGGCCATCAAGAAGGGGGTTGATCCAATCCCTGTTCATGGTGGGCTAGGTGGTGCTTACTACTTCAGGAACATCAGTGGAGAGAGTGTTGCCATCGTGAAGCCAACAGATGAGGAGCCATTTGCACCAAACAACCCTAAAGGCTTTGTAGGCAAAGCTCTTGGGCAGCCTGGCCTGAAGAGTTCAGTGCGAGTTGGGGAAACAGGGTTTCGAGAAGTTGCTGCCTACCTTCTGGATTATGATCACTTTGCTAATGTGCCTCCAACTGTTCTTGTGAAGGTTGCACATCAAATCTTCAATGTTAATTGTGGTGTCAATGGGGACAAGATTCAGAAGAAGAAAAGGGTTAGCAAAATTGCATCACTGCAGCAGTTCATCCTTCATGACTATGATGCTGGTGACTGTGGAACCTCAAGTTTTCCAGTAGCAGCTGTTCATAGAATAGGCATTTTAGATATTAGGATCTTTAACACTGACAGGCATGCAGGGAACCTGCTGGTTAAAAAGCTTGATGGGGAGTTTGGTCAGGTGGAGCTCATTCCAATTGATCATGGCCTTTGTTTGCCAGAGCACTTGGAAGATCCATATTTTGAGTGGATTCATTGGCCTCAGGCATCAATTCCTTTCTCTGAGGATGAGCTTGAGTATATAAGGAATCTCGATCCAATTTGTGATTCTGAGATGCTAAAGATGGAGCTGCCTATGATTCGGGAGGCATGCCTTAGAGTTCTGGTCCTGTGCACAATATTCCTCAAGGAAGCAACGGCTTTTGGACTGTGTCTTGCTGAGATTGGTGAGATGATGAGTAGGGAGTTCGGAGGCCGAGAAGAGAAGCCAAGCGAACTTGAGGCTGTATGCCTTGAGGCCAGGAGGATTATAGAAGATTTAAACATGTCAAACGTTGAGGCTGAAGAAGGAAACGAAGAGTTTCTTTTCGATATAGATTGTGAGAATGCAATGATTGTAGACCCTTCACAAGTGATCTCAAGAGACTTCTTTGACCAGGTTTCATATCCTACCCGAACAAGCATTGTGAAGGTTGCTGATATAAGGCCAGTCAGGCCAGAGGAAAATTGGTATAAAACTGCTTCAAAATTGCCCACGTCTCTGAAAAATATTGATGTGGGTGTCAGAAGAAGTGCAAATGATCAGTTACCTACCTCAATAAGCTTTGTGAAGGTTGCTGATATGAGTGAGGAGCAATGGATTCTGTTTCTTGAAAAGGTCAGGGAGCTTCTGTACCCTGCCTTTGGCAACCGAAGGCGTGCAACCATTGGGCAGAGACAAAGGCTGGGATCATCTTGCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

590

Amino Acids

65.85

Weight (kDa)

5.82

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 957
AccI GTMKAC 1 cut(s) 1422
AciI CCGC 2 cut(s) 283, 458
AclI AACGTT 1 cut(s) 1356
AclWI GGATC 8 cut(s) 258, 383, 387, 473, 926, 1031, 1112, 1759
AcoI YGGCCR 1 cut(s) 459
AcuI CTGAAG 3 cut(s) 505, 653, 1386
AfaI GTAC 2 cut(s) 219, 1697
AfiI CCNNNNNNNGG 2 cut(s) 494, 1706
AflIII ACRYGT 2 cut(s) 115, 1347
AgsI TTSAA 3 cut(s) 754, 1548, 1676
AhdI GACNNNNNGTC 1 cut(s) 342
AjiI CACGTC 2 cut(s) 116, 1560
AjnI CCWGG 3 cut(s) 625, 1322, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
Alw21I GWGCWC 5 cut(s) 89, 236, 993, 1029, 1199
Alw26I GTCTC 6 cut(s) 106, 128, 350, 1440, 1566, 1732
Alw44I GTGCAC 1 cut(s) 1195
AlwI GGATC 8 cut(s) 258, 383, 387, 473, 926, 1031, 1112, 1759
AoxI GGCC 8 cut(s) 459, 628, 1009, 1061, 1276, 1320, 1508, 1517
ApaLI GTGCAC 1 cut(s) 1195
ApeKI GCWGC 7 cut(s) 61, 202, 622, 677, 823, 884, 1153
Asp700I GAANNNNTTC 1 cut(s) 1383
AspS9I GGNCC 1 cut(s) 1189
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 4 cut(s) 182, 342, 866, 1262
AvaII GGWCC 1 cut(s) 1189
AxyI CCTNAGG 1 cut(s) 1065
BaeGI GKGCMC 1 cut(s) 1199
BanII GRGCYC 2 cut(s) 236, 993
Bbv12I GWGCWC 5 cut(s) 89, 236, 993, 1029, 1199
BbvI GCAGC 7 cut(s) 48, 214, 634, 664, 835, 896, 1140
BccI CCATC 6 cut(s) 31, 287, 470, 557, 962, 1141
BceAI ACGGC 1 cut(s) 1236
BciT130I CCWGG 3 cut(s) 627, 1324, 1460
BclI TGATCA 3 cut(s) 697, 1003, 1603
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 6 cut(s) 106, 128, 350, 1440, 1566, 1732
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 1 cut(s) 821
BfuAI ACCTGC 1 cut(s) 957
BglI GCCNNNNNGGC 1 cut(s) 1516
BisI GCNGC 8 cut(s) 62, 203, 459, 623, 678, 824, 885, 1154
BlsI GCNGC 8 cut(s) 63, 204, 460, 624, 679, 825, 886, 1155
Bme1390I CCNGG 4 cut(s) 106, 627, 1324, 1460
Bme18I GGWCC 1 cut(s) 1189
BmeRI GACNNNNNGTC 1 cut(s) 342
BmgBI CACGTC 2 cut(s) 116, 1560
BmgT120I GGNCC 1 cut(s) 1189
BmiI GGNNCC 3 cut(s) 573, 865, 947
BmrFI CCNGG 4 cut(s) 106, 627, 1324, 1460
BmsI GCATC 4 cut(s) 824, 838, 1079, 1128
BpmI CTGGAG 1 cut(s) 356
BpuEI CTTGAG 6 cut(s) 853, 1118, 1193, 1322, 1337, 1426
BpuMI CCSGG 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 696
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 528, 558, 1679, 1709
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 1706
Bse1I ACTGG 4 cut(s) 142, 444, 878, 1511
Bse21I CCTNAGG 1 cut(s) 1065
Bse3DI GCAATG 2 cut(s) 1419, 1664
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 3 cut(s) 627, 1324, 1460
BseGI GGATG 3 cut(s) 279, 831, 1096
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 2 cut(s) 494, 1706
BseMI GCAATG 2 cut(s) 1419, 1664
BseMII CTCAG 6 cut(s) 123, 226, 1077, 1079, 1125, 1233
BseNI ACTGG 4 cut(s) 142, 444, 878, 1511
BseRI GAGGAG 3 cut(s) 124, 584, 1667
BseSI GKGCMC 1 cut(s) 1199
BseXI GCAGC 7 cut(s) 48, 214, 634, 664, 835, 896, 1140
BseYI CCCAGC 1 cut(s) 1747
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 8 cut(s) 461, 630, 1011, 1063, 1278, 1322, 1510, 1519
BsiHKAI GWGCWC 5 cut(s) 89, 236, 993, 1029, 1199
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 2 cut(s) 494, 1706
BsmAI GTCTC 6 cut(s) 106, 128, 350, 1440, 1566, 1732
BsmBI CGTCTC 2 cut(s) 106, 1566
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 1414
BsnI GGCC 8 cut(s) 461, 630, 1011, 1063, 1278, 1322, 1510, 1519
Bsp1286I GDGCHC 5 cut(s) 89, 236, 993, 1029, 1199
BspACI CCGC 2 cut(s) 283, 458
BspANI GGCC 8 cut(s) 461, 630, 1011, 1063, 1278, 1322, 1510, 1519
BspCNI CTCAG 6 cut(s) 124, 225, 1078, 1078, 1126, 1234
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 573, 865, 947
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 957
BspPI GGATC 8 cut(s) 258, 383, 387, 473, 926, 1031, 1112, 1759
BsrDI GCAATG 2 cut(s) 1419, 1664
BsrI ACTGG 4 cut(s) 142, 444, 878, 1511
Bst2UI CCWGG 3 cut(s) 627, 1324, 1460
Bst4CI ACNGT 4 cut(s) 187, 724, 860, 1233
Bst6I CTCTTC 3 cut(s) 629, 1278, 1374
BstAPI GCANNNNNTGC 1 cut(s) 314
BstC8I GCNNGC 4 cut(s) 207, 939, 1174, 1722
BstDEI CTNAG 8 cut(s) 132, 212, 272, 1065, 1086, 1134, 1178, 1242
BstF5I GGATG 3 cut(s) 279, 831, 1096
BstMAI GTCTC 6 cut(s) 106, 128, 350, 1440, 1566, 1732
BstMWI GCNNNNNNNGC 3 cut(s) 314, 606, 1516
BstNI CCWGG 3 cut(s) 627, 1324, 1460
BstNSI RCATGY 3 cut(s) 941, 1176, 1351
BstSCI CCNGG 4 cut(s) 104, 625, 1322, 1458
BstSFI CTRYAG 1 cut(s) 821
BstSLI GKGCMC 1 cut(s) 1199
BstV1I GCAGC 7 cut(s) 48, 214, 634, 664, 835, 896, 1140
BstX2I RGATCY 2 cut(s) 918, 1036
BstYI RGATCY 2 cut(s) 918, 1036
Bsu36I CCTNAGG 1 cut(s) 1065
BsuRI GGCC 8 cut(s) 461, 630, 1011, 1063, 1278, 1322, 1510, 1519
BtrI CACGTC 2 cut(s) 116, 1560
BtsCI GGATG 3 cut(s) 279, 831, 1096
BtsI GCAGTG 2 cut(s) 296, 818
BtsIMutI CAGTG 7 cut(s) 61, 220, 296, 538, 648, 818, 927
BveI ACCTGC 1 cut(s) 957
Cac8I GCNNGC 4 cut(s) 207, 939, 1174, 1722
CciI TCATGA 1 cut(s) 838
Cfr13I GGNCC 1 cut(s) 1189
CsiI ACCWGGT 1 cut(s) 1458
Csp6I GTAC 2 cut(s) 218, 1696
CviAII CATG 8 cut(s) 80, 319, 494, 839, 938, 1007, 1173, 1348
CviQI GTAC 2 cut(s) 218, 1696
DdeI CTNAG 8 cut(s) 132, 212, 272, 1065, 1086, 1134, 1178, 1242
DraI TTTAAA 1 cut(s) 1344
DriI GACNNNNNGTC 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 629, 1278, 1374
Eam1105I GACNNNNNGTC 1 cut(s) 342
EarI CTCTTC 3 cut(s) 629, 1278, 1374
Ecl136II GAGCTC 2 cut(s) 234, 991
Eco24I GRGCYC 2 cut(s) 236, 993
Eco47I GGWCC 1 cut(s) 1189
Eco53kI GAGCTC 2 cut(s) 234, 991
Eco57I CTGAAG 3 cut(s) 505, 653, 1386
Eco81I CCTNAGG 1 cut(s) 1065
EcoICRI GAGCTC 2 cut(s) 234, 991
EcoRII CCWGG 3 cut(s) 625, 1322, 1458
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 236, 993
Esp3I CGTCTC 2 cut(s) 106, 1566
FaeI CATG 8 cut(s) 83, 322, 497, 842, 941, 1010, 1176, 1351
FalI AAGNNNNNCTT 2 cut(s) 1371, 1403
FaqI GGGAC 1 cut(s) 791
FatI CATG 8 cut(s) 79, 318, 493, 838, 937, 1006, 1172, 1347
FbaI TGATCA 3 cut(s) 697, 1003, 1603
FblI GTMKAC 1 cut(s) 1422
Fnu4HI GCNGC 8 cut(s) 62, 203, 459, 623, 678, 824, 885, 1154
FokI GGATG 3 cut(s) 266, 818, 1103
FriOI GRGCYC 2 cut(s) 236, 993
Fsp4HI GCNGC 8 cut(s) 62, 203, 459, 623, 678, 824, 885, 1154
FspBI CTAG 1 cut(s) 503
GluI GCNGC 8 cut(s) 62, 203, 459, 623, 678, 824, 885, 1154
GsaI CCCAGC 1 cut(s) 1751
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 8 cut(s) 461, 630, 1011, 1063, 1278, 1322, 1510, 1519
HapII CCGG 1 cut(s) 106
Hin1II CATG 8 cut(s) 83, 322, 497, 842, 941, 1010, 1176, 1351
HindIII AAGCTT 2 cut(s) 962, 1624
HpaII CCGG 1 cut(s) 106
HphI GGTGA 4 cut(s) 182, 342, 866, 1262
Hpy166II GTNNAC 3 cut(s) 218, 1197, 1423
Hpy188I TCNGA 6 cut(s) 789, 1087, 1135, 1274, 1566, 1589
Hpy8I GTNNAC 3 cut(s) 218, 1197, 1423
HpyCH4III ACNGT 4 cut(s) 187, 724, 860, 1233
HpyCH4IV ACGT 3 cut(s) 115, 1356, 1559
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 606, 1516
HpyF3I CTNAG 8 cut(s) 132, 212, 272, 1065, 1086, 1134, 1178, 1242
HpySE526I ACGT 3 cut(s) 115, 1356, 1559
Hsp92II CATG 8 cut(s) 83, 322, 497, 842, 941, 1010, 1176, 1351
Ksp22I TGATCA 3 cut(s) 697, 1003, 1603
LmnI GCTCC 5 cut(s) 571, 988, 1150, 1654, 1687
Lsp1109I GCAGC 7 cut(s) 48, 214, 634, 664, 835, 896, 1140
LweI GCATC 4 cut(s) 824, 838, 1079, 1128
MabI ACCWGGT 1 cut(s) 1458
MaeI CTAG 1 cut(s) 503
MaeII ACGT 3 cut(s) 115, 1356, 1559
MaeIII GTNAC 4 cut(s) 43, 170, 854, 1608
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 2 cut(s) 918, 1036
MhlI GDGCHC 5 cut(s) 89, 236, 993, 1029, 1199
MluCI AATT 8 cut(s) 429, 760, 810, 999, 1074, 1122, 1528, 1550
MlyI GAGTC 2 cut(s) 28, 331
MmeI TCCRAC 2 cut(s) 141, 743
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 1383
MseI TTAA 7 cut(s) 27, 260, 444, 759, 924, 957, 1343
MslI CAYNNNNRTG 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 887
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 4 cut(s) 106, 627, 1324, 1460
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 1 cut(s) 1414
MvaI CCWGG 3 cut(s) 627, 1324, 1460
MwoI GCNNNNNNNGC 3 cut(s) 314, 606, 1516
NciI CCSGG 1 cut(s) 106
NlaIII CATG 8 cut(s) 83, 322, 497, 842, 941, 1010, 1176, 1351
NlaIV GGNNCC 3 cut(s) 573, 865, 947
NmeAIII GCCGAG 1 cut(s) 1304
NmuCI GTSAC 3 cut(s) 43, 170, 854
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 3 cut(s) 941, 1176, 1351
PaeI GCATGC 2 cut(s) 941, 1176
PagI TCATGA 1 cut(s) 838
PciI ACATGT 1 cut(s) 1347
PctI GAATGC 1 cut(s) 1414
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 8 cut(s) 74, 364, 784, 1054, 1111, 1130, 1162, 1663
PflFI GACNNNGTC 1 cut(s) 1233
PkrI GCNGC 8 cut(s) 63, 204, 460, 624, 679, 825, 886, 1155
PleI GAGTC 2 cut(s) 27, 331
PpsI GAGTC 2 cut(s) 27, 331
PscI ACATGT 1 cut(s) 1347
Psp124BI GAGCTC 2 cut(s) 236, 993
Psp1406I AACGTT 1 cut(s) 1356
Psp6I CCWGG 3 cut(s) 625, 1322, 1458
PspFI CCCAGC 1 cut(s) 1747
PspGI CCWGG 3 cut(s) 625, 1322, 1458
PspN4I GGNNCC 3 cut(s) 573, 865, 947
PspPI GGNCC 1 cut(s) 1189
PsrI GAACNNNNNNTAC 2 cut(s) 873, 905
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 2 cut(s) 918, 1036
PsyI GACNNNGTC 1 cut(s) 1233
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 2 cut(s) 219, 1697
RsaNI GTAC 2 cut(s) 218, 1696
RseI CAYNNNNRTG 1 cut(s) 843
SacI GAGCTC 2 cut(s) 236, 993
SaqAI TTAA 7 cut(s) 27, 260, 444, 759, 924, 957, 1343
SatI GCNGC 8 cut(s) 62, 203, 459, 623, 678, 824, 885, 1154
Sau96I GGNCC 1 cut(s) 1189
SchI GAGTC 2 cut(s) 28, 331
ScrFI CCNGG 4 cut(s) 106, 627, 1324, 1460
SduI GDGCHC 5 cut(s) 89, 236, 993, 1029, 1199
SexAI ACCWGGT 1 cut(s) 1458
SfaNI GCATC 4 cut(s) 824, 838, 1079, 1128
SfcI CTRYAG 1 cut(s) 821
SfiI GGCCNNNNNGGCC 1 cut(s) 1516
SinI GGWCC 1 cut(s) 1189
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 6 cut(s) 868, 1097, 1208, 1301, 1316, 1441
SmoI CTYRAG 6 cut(s) 868, 1097, 1208, 1301, 1316, 1441
SphI GCATGC 2 cut(s) 941, 1176
Sse9I AATT 8 cut(s) 429, 760, 810, 999, 1074, 1122, 1528, 1550
SsiI CCGC 2 cut(s) 283, 458
SspI AATATT 2 cut(s) 1203, 1573
SspMI CTAG 1 cut(s) 503
SstI GAGCTC 2 cut(s) 236, 993
StyD4I CCNGG 4 cut(s) 104, 625, 1322, 1458
TaaI ACNGT 4 cut(s) 187, 724, 860, 1233
TaiI ACGT 3 cut(s) 118, 1359, 1562
TaqI TCGA 4 cut(s) 335, 667, 1116, 1392
TasI AATT 8 cut(s) 429, 760, 810, 999, 1074, 1122, 1528, 1550
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 8 cut(s) 74, 364, 784, 1054, 1111, 1130, 1162, 1663
Tru1I TTAA 7 cut(s) 27, 260, 444, 759, 924, 957, 1343
Tru9I TTAA 7 cut(s) 27, 260, 444, 759, 924, 957, 1343
TscAI CASTG 7 cut(s) 61, 220, 303, 538, 648, 825, 934
TseFI GTSAC 3 cut(s) 43, 170, 854
TseI GCWGC 7 cut(s) 61, 202, 622, 677, 823, 884, 1153
Tsp45I GTSAC 3 cut(s) 43, 170, 854
TspDTI ATGAA 7 cut(s) 450, 482, 820, 827, 881, 1046, 1455
TspRI CASTG 7 cut(s) 61, 220, 303, 538, 648, 825, 934
Tth111I GACNNNGTC 1 cut(s) 1233
VneI GTGCAC 1 cut(s) 1195
VpaK11BI GGWCC 1 cut(s) 1189
XceI RCATGY 3 cut(s) 941, 1176, 1351
XcmI CCANNNNNNNNNTGG 2 cut(s) 1047, 1527
XmiI GTMKAC 1 cut(s) 1422
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.