MD14G1217200.v1.1

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013673
Physical Location & Seq
Forward (+)
30076738 .. 30079882
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1217200.v1.1.491

Sequence Viewer

Length: 1974 bp
ATGAGTGATTCGCGGCCATTTGTCTCTCTCGTTCAATTGGTGTCATCGATTTTGACACCTGCTGAGAGAGTTGAGGATTTGCACCCTGATATCGTATCAACCGCGCGATTCAGTAAGGATTTTCATCCTTATTTGATGGCTACAAAGTTGGAGAGTCCTGTTAAGGCCCAGATGGCTGTGACACTGCTGAATGGCGACTGCCTTGAGAGGGCGAGAGGCAGTCTTGCTCGGAGGAGGCGTGTCTTTGTGCAGACTGAGACTGGGTGTGTTTTGGGAATGGAGTTGGAAAGGGGTGACAATGCCCACACGGTTAAAAGGAGGTTGCAGCTTGCCCTCAATGTACCTACGGACCAGAGCTCTTTGACCTTTGGGGATCGTATATTGAACAATGATCTTAGTGCCATTGGCAATCGTTCAACGCTACTACTTGCTAGGAATGTCATGAACAGAAGCTCGTCAACTCCTTGTCTGTCACCTACTGGGCAAAATCTGCAGAGGGATCAGAGTGAGTCTATTGAGGTTTTGGGGTGTCCAAATAATTTTGCGAGAATTGATGAACTGGTTAAGGAGATTGTGGTTGCTATCAAGAAGGGTGTTGATCCGATCCCCGTGCATAGTGGGCTTGGTGGTGCCTACTACTTCAGGAACATCATGGGCGAGAGTGTTGCCATCGTGAAGCCAACAGATGAGGAGCCATTTGCACCGAACAACCCTAAAGGCTTTGTAGGCAAGGCACTCGGGCAGCCTGGCCTGAAGCGTTCAGTGCGAGTTGGGGAAACGGGGTTTCGAGAAGTTGCTGCCTACCTTCTGGATTATGATCACTTTGCTAATGTGCCTCCAACTGTTCTTGTGAAGGCTGCACATCAAATCTTCAATGTTAATTATGGCGTCAATGGAGATAAGATTCAGAAGAAGAAAAGGGTTAGCAAGATTGCGTCTCTGCAGGTCTTCATCCCTCATGACTTTGATGCCAGTGACTACGGAACTTCAAGCTTTCCCGTATCAGCCGTTCATAGAATAGGCATTTTAGACATTAGGATCTTTAACACTGACAGGCATGCTGGGAACCTTCTGGTTAAAAAGCTTGATGGGGAGTTTGGTAAAGTGGAGCTCATTCCAATTGACCACGGACTTTGCTTGCCGGAGCACTTGGAAGATCCATATTTTGAGTGGATTCATTGGCCTCAGGCATCAATTCCTTTCTCTGAGGATGAGCTTGAGTATATACGTAATCTTAATCCAATTTGTGACTCCGAGATGCTGAAAATGGAGCTGCCCATGATCCGAGAGGCATGCCTTAGAGTTCTGGTCCTGTGCACAATATTTCTCAAGGAAGCTGCTGCTTTTGGACTCTGCCTTGCTGAGATTGGTGAGATGATGAGTAGGGAGTTTGGAGGACAAGAGGAGAAACCAAGCAAACTTGAAGCTGTATGCCTCGAGGCCAGGAAGATCATAGCAGATTTGAACTTGTCAACCTTTGAGGCGGAAGAAGGAAATGAAGAGTTTCTGTTTGATATAGATTGTGAGAATGCTGGGATTCTTGATCCTTCAGACGTTATGTCACGTCACTTCTTTGATCAGGTTTCATATCCTTTTGGGTCTAGAGGCGCTGATGGTCGAAACTTGCTGTCCAAGCTAGATGAGTGTTTGGAAGAGGATGAAGTTGGGGCAGACGGAATCACTATCCAGCCAAATTTGTATCAAACTGCTTCAAAATTGCCCCTTCCTCCGAAAAGAATAGATGTGGATGGGAGAAGCCGGCTCCAGCGAGCTGCAACCATATCCGGAATCAGAAGAAGTGCAAATGATCAGTTACCAACCTCACCATGCTTCGTGAAGGTTGCTGACATGAGTGAGGAGGAATGGATTCTGTTCCTTGAAAAGTTCAGGGAGCTTCTTTACCCCGCCTTTGGCAACCAAAGGTCTGCAACCCTCAGACAGAGACAGAGACTTGGAACATCTTGCAGATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

658

Amino Acids

73.27

Weight (kDa)

5.57

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 209 - 455 7.2e-44 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 67
AbsI CCTCGAGG 1 cut(s) 1436
Acc36I ACCTGC 2 cut(s) 67, 934
AccB1I GGYRCC 1 cut(s) 629
AccII CGCG 3 cut(s) 13, 104, 106
AccIII TCCGGA 1 cut(s) 1784
AciI CCGC 4 cut(s) 13, 102, 1484, 1905
AclWI GGATC 8 cut(s) 381, 507, 593, 598, 1046, 1151, 1276, 1538
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 1 cut(s) 1693
AcuI CTGAAG 3 cut(s) 625, 773, 1533
AcyI GRCGYC 1 cut(s) 888
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 208, 1910
AgsI TTSAA 9 cut(s) 35, 385, 417, 874, 990, 1424, 1465, 1713, 1880
AhdI GACNNNNNGTC 1 cut(s) 1558
AjiI CACGTC 1 cut(s) 1565
AjnI CCWGG 2 cut(s) 745, 1442
Alw21I GWGCWC 4 cut(s) 359, 1113, 1149, 1319
Alw26I GTCTC 5 cut(s) 28, 251, 942, 1936, 1942
Alw44I GTGCAC 1 cut(s) 1315
AlwI GGATC 8 cut(s) 381, 507, 593, 598, 1046, 1151, 1276, 1538
Ama87I CYCGRG 2 cut(s) 737, 1436
Aor13HI TCCGGA 1 cut(s) 1784
AoxI GGCC 5 cut(s) 14, 165, 748, 1181, 1440
ApaLI GTGCAC 1 cut(s) 1315
ApeKI GCWGC 8 cut(s) 325, 742, 797, 857, 1273, 1337, 1340, 1772
ApoI RAATTY 1 cut(s) 1693
Asp700I GAANNNNTTC 2 cut(s) 1503, 1866
AspLEI GCGC 2 cut(s) 106, 1610
AspS9I GGNCC 3 cut(s) 166, 349, 1309
AsuHPI GGTGA 4 cut(s) 305, 465, 1382, 1815
AvaI CYCGRG 2 cut(s) 737, 1436
AvaII GGWCC 2 cut(s) 349, 1309
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 1319
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 2 cut(s) 359, 1113
BbsI GAAGAC 1 cut(s) 940
Bbv12I GWGCWC 4 cut(s) 359, 1113, 1149, 1319
BbvI GCAGC 8 cut(s) 337, 754, 784, 844, 1260, 1324, 1327, 1759
BccI CCATC 6 cut(s) 130, 166, 677, 1082, 1607, 1742
BceAI ACGGC 1 cut(s) 992
BcgI CGANNNNNNTGC 8 cut(s) 592, 626, 647, 681, 718, 752, 1275, 1309
BciT130I CCWGG 2 cut(s) 747, 1444
BclI TGATCA 3 cut(s) 817, 1576, 1807
BcoDI GTCTC 5 cut(s) 28, 251, 942, 1936, 1942
BfaI CTAG 3 cut(s) 432, 1602, 1637
BfmI CTRYAG 2 cut(s) 491, 941
BfoI RGCGCY 1 cut(s) 1611
BfuAI ACCTGC 2 cut(s) 67, 934
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 9 cut(s) 14, 326, 743, 798, 858, 1274, 1338, 1341, 1773
BlsI GCNGC 9 cut(s) 15, 327, 744, 799, 859, 1275, 1339, 1342, 1774
Bme1390I CCNGG 2 cut(s) 747, 1444
Bme18I GGWCC 2 cut(s) 349, 1309
BmeRI GACNNNNNGTC 1 cut(s) 1558
BmeT110I CYCGRG 2 cut(s) 737, 1436
BmgBI CACGTC 1 cut(s) 1565
BmgT120I GGNCC 3 cut(s) 166, 349, 1309
BmiI GGNNCC 4 cut(s) 631, 693, 1067, 1763
BmrFI CCNGG 2 cut(s) 747, 1444
BmrI ACTGGG 2 cut(s) 270, 489
BmsI GCATC 3 cut(s) 958, 1199, 1248
BmuI ACTGGG 2 cut(s) 270, 489
BpiI GAAGAC 1 cut(s) 940
BpmI CTGGAG 1 cut(s) 1748
BpuEI CTTGAG 3 cut(s) 224, 1238, 1313
Bsa29I ATCGAT 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 1229
BsaBI GATNNNNATC 2 cut(s) 123, 816
BsaHI GRCGYC 1 cut(s) 888
BsaJI CCNNGG 1 cut(s) 1126
BsaWI WCCGGW 1 cut(s) 1784
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 1910
Bse118I RCCGGY 1 cut(s) 1758
Bse1I ACTGG 4 cut(s) 265, 484, 564, 972
Bse21I CCTNAGG 1 cut(s) 1185
Bse8I GATNNNNATC 2 cut(s) 123, 816
BseAI TCCGGA 1 cut(s) 1784
BseBI CCWGG 2 cut(s) 747, 1444
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 1126
BseGI GGATG 5 cut(s) 124, 951, 1216, 1663, 1753
BseJI GATNNNNATC 2 cut(s) 123, 816
BseLI CCNNNNNNNGG 2 cut(s) 208, 1910
BseMII CTCAG 6 cut(s) 54, 246, 1197, 1199, 1353, 1948
BseNI ACTGG 4 cut(s) 265, 484, 564, 972
BseRI GAGGAG 4 cut(s) 247, 704, 1418, 1871
BseSI GKGCMC 1 cut(s) 1319
BseXI GCAGC 8 cut(s) 337, 754, 784, 844, 1260, 1324, 1327, 1759
BseYI CCCAGC 2 cut(s) 1061, 1532
BsgI GTGCAG 2 cut(s) 269, 843
Bsh1236I CGCG 3 cut(s) 13, 104, 106
BshFI GGCC 5 cut(s) 16, 167, 750, 1183, 1442
BshNI GGYRCC 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 47
BsiHKAI GWGCWC 4 cut(s) 359, 1113, 1149, 1319
BsiHKCI CYCGRG 2 cut(s) 737, 1436
BsiSI CCGG 3 cut(s) 1142, 1759, 1785
BslI CCNNNNNNNGG 2 cut(s) 208, 1910
BsmAI GTCTC 5 cut(s) 28, 251, 942, 1936, 1942
BsmBI CGTCTC 1 cut(s) 942
BsmI GAATGC 1 cut(s) 1534
BsnI GGCC 5 cut(s) 16, 167, 750, 1183, 1442
BsoBI CYCGRG 2 cut(s) 737, 1436
Bsp1286I GDGCHC 4 cut(s) 359, 1113, 1149, 1319
Bsp13I TCCGGA 1 cut(s) 1784
BspACI CCGC 4 cut(s) 13, 102, 1484, 1905
BspANI GGCC 5 cut(s) 16, 167, 750, 1183, 1442
BspCNI CTCAG 6 cut(s) 55, 247, 1198, 1198, 1354, 1947
BspDI ATCGAT 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 1784
BspFNI CGCG 3 cut(s) 13, 104, 106
BspHI TCATGA 2 cut(s) 441, 958
BspLI GGNNCC 4 cut(s) 631, 693, 1067, 1763
BspMAI CTGCAG 2 cut(s) 495, 945
BspMI ACCTGC 2 cut(s) 67, 934
BspPI GGATC 8 cut(s) 381, 507, 593, 598, 1046, 1151, 1276, 1538
BspT107I GGYRCC 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 1758
BsrI ACTGG 4 cut(s) 265, 484, 564, 972
BssAI RCCGGY 1 cut(s) 1758
BssECI CCNNGG 1 cut(s) 1126
BssNI GRCGYC 1 cut(s) 888
Bst2UI CCWGG 2 cut(s) 747, 1444
Bst4CI ACNGT 2 cut(s) 310, 844
Bst6I CTCTTC 2 cut(s) 1494, 1647
BstACI GRCGYC 1 cut(s) 888
BstAPI GCANNNNNTGC 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 1229
BstC8I GCNNGC 6 cut(s) 330, 1059, 1139, 1294, 1760, 1770
BstDEI CTNAG 8 cut(s) 63, 255, 395, 1185, 1206, 1298, 1362, 1934
BstDSI CCRYGG 1 cut(s) 1126
BstF5I GGATG 5 cut(s) 124, 951, 1216, 1663, 1753
BstFNI CGCG 3 cut(s) 13, 104, 106
BstH2I RGCGCY 1 cut(s) 1611
BstHHI GCGC 2 cut(s) 106, 1610
BstMAI GTCTC 5 cut(s) 28, 251, 942, 1936, 1942
BstMWI GCNNNNNNNGC 6 cut(s) 173, 490, 619, 726, 763, 1633
BstNI CCWGG 2 cut(s) 747, 1444
BstNSI RCATGY 2 cut(s) 1061, 1296
BstSCI CCNGG 2 cut(s) 745, 1442
BstSFI CTRYAG 2 cut(s) 491, 941
BstSLI GKGCMC 1 cut(s) 1319
BstSNI TACGTA 1 cut(s) 1229
BstUI CGCG 3 cut(s) 13, 104, 106
BstV1I GCAGC 8 cut(s) 337, 754, 784, 844, 1260, 1324, 1327, 1759
BstV2I GAAGAC 1 cut(s) 940
BstX2I RGATCY 2 cut(s) 1038, 1156
BstYI RGATCY 2 cut(s) 1038, 1156
Bsu15I ATCGAT 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 1185
BsuRI GGCC 5 cut(s) 16, 167, 750, 1183, 1442
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 1126
BtrI CACGTC 1 cut(s) 1565
BtsCI GGATG 5 cut(s) 124, 951, 1216, 1663, 1753
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 4 cut(s) 182, 768, 979, 1047
BveI ACCTGC 2 cut(s) 67, 934
Cac8I GCNNGC 6 cut(s) 330, 1059, 1139, 1294, 1760, 1770
CciI TCATGA 2 cut(s) 441, 958
CfoI GCGC 2 cut(s) 106, 1610
Cfr10I RCCGGY 1 cut(s) 1758
Cfr13I GGNCC 3 cut(s) 166, 349, 1309
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 2 cut(s) 877, 924
Csp6I GTAC 1 cut(s) 341
CspCI CAANNNNNGTGG 2 cut(s) 1115, 1150
CviAII CATG 8 cut(s) 442, 652, 959, 1058, 1279, 1293, 1827, 1849
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 8 cut(s) 63, 255, 395, 1185, 1206, 1298, 1362, 1934
DriI GACNNNNNGTC 1 cut(s) 1558
EaeI YGGCCR 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 1494, 1647
Eam1105I GACNNNNNGTC 1 cut(s) 1558
EarI CTCTTC 2 cut(s) 1494, 1647
EciI GGCGGA 1 cut(s) 1499
Ecl136II GAGCTC 2 cut(s) 357, 1111
Eco105I TACGTA 1 cut(s) 1229
Eco24I GRGCYC 2 cut(s) 359, 1113
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 349, 1309
Eco53kI GAGCTC 2 cut(s) 357, 1111
Eco57I CTGAAG 3 cut(s) 625, 773, 1533
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 2 cut(s) 737, 1436
EcoICRI GAGCTC 2 cut(s) 357, 1111
EcoRII CCWGG 2 cut(s) 745, 1442
EcoRV GATATC 1 cut(s) 91
EcoT38I GRGCYC 2 cut(s) 359, 1113
Esp3I CGTCTC 1 cut(s) 942
FaeI CATG 8 cut(s) 445, 655, 962, 1061, 1282, 1296, 1830, 1852
FatI CATG 8 cut(s) 441, 651, 958, 1057, 1278, 1292, 1826, 1848
FauI CCCGC 1 cut(s) 1912
FbaI TGATCA 3 cut(s) 817, 1576, 1807
Fnu4HI GCNGC 9 cut(s) 14, 326, 743, 798, 858, 1274, 1338, 1341, 1773
FokI GGATG 5 cut(s) 111, 938, 1223, 1670, 1760
FriOI GRGCYC 2 cut(s) 359, 1113
Fsp4HI GCNGC 9 cut(s) 14, 326, 743, 798, 858, 1274, 1338, 1341, 1773
FspBI CTAG 3 cut(s) 432, 1602, 1637
GlaI GCGC 2 cut(s) 105, 1609
GluI GCNGC 9 cut(s) 14, 326, 743, 798, 858, 1274, 1338, 1341, 1773
GsaI CCCAGC 2 cut(s) 1065, 1536
GsuI CTGGAG 1 cut(s) 1748
HaeII RGCGCY 1 cut(s) 1611
HaeIII GGCC 5 cut(s) 16, 167, 750, 1183, 1442
HapII CCGG 3 cut(s) 1142, 1759, 1785
HgaI GACGC 2 cut(s) 877, 924
HhaI GCGC 2 cut(s) 106, 1610
Hin1I GRCGYC 1 cut(s) 888
Hin1II CATG 8 cut(s) 445, 655, 962, 1061, 1282, 1296, 1830, 1852
Hin6I GCGC 2 cut(s) 104, 1608
HinP1I GCGC 2 cut(s) 104, 1608
HincII GTYRAC 2 cut(s) 459, 1473
HindII GTYRAC 2 cut(s) 459, 1473
HindIII AAGCTT 2 cut(s) 991, 1082
HpaII CCGG 3 cut(s) 1142, 1759, 1785
HphI GGTGA 4 cut(s) 305, 465, 1382, 1815
Hpy166II GTNNAC 3 cut(s) 459, 1317, 1473
Hpy8I GTNNAC 3 cut(s) 459, 1317, 1473
HpyAV CCTTC 8 cut(s) 583, 815, 847, 1079, 1484, 1557, 1733, 1831
HpyCH4III ACNGT 2 cut(s) 310, 844
HpyCH4IV ACGT 3 cut(s) 1228, 1554, 1564
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 490, 619, 726, 763, 1633
HpyF3I CTNAG 8 cut(s) 63, 255, 395, 1185, 1206, 1298, 1362, 1934
HpySE526I ACGT 3 cut(s) 1228, 1554, 1564
Hsp92I GRCGYC 1 cut(s) 888
Hsp92II CATG 8 cut(s) 445, 655, 962, 1061, 1282, 1296, 1830, 1852
HspAI GCGC 2 cut(s) 104, 1608
Kpn2I TCCGGA 1 cut(s) 1784
KroI GCCGGC 1 cut(s) 1758
KroNI GCCGGC 1 cut(s) 1760
Ksp22I TGATCA 3 cut(s) 817, 1576, 1807
LmnI GCTCC 6 cut(s) 691, 1108, 1144, 1270, 1767, 1891
Lsp1109I GCAGC 8 cut(s) 337, 754, 784, 844, 1260, 1324, 1327, 1759
LweI GCATC 3 cut(s) 958, 1199, 1248
MaeI CTAG 3 cut(s) 432, 1602, 1637
MaeII ACGT 3 cut(s) 1228, 1554, 1564
MaeIII GTNAC 8 cut(s) 178, 293, 471, 974, 1247, 1560, 1565, 1812
MfeI CAATTG 2 cut(s) 35, 1119
MflI RGATCY 2 cut(s) 1038, 1156
MhlI GDGCHC 4 cut(s) 359, 1113, 1149, 1319
MluCI AATT 9 cut(s) 35, 538, 549, 880, 1119, 1194, 1242, 1693, 1715
MlyI GAGTC 4 cut(s) 163, 518, 1244, 1344
MmeI TCCRAC 3 cut(s) 129, 264, 863
MroI TCCGGA 1 cut(s) 1784
MroNI GCCGGC 1 cut(s) 1758
MroXI GAANNNNTTC 2 cut(s) 1503, 1866
MseI TTAA 8 cut(s) 162, 312, 564, 879, 1044, 1077, 1236, 1972
MspI CCGG 3 cut(s) 1142, 1759, 1785
MspR9I CCNGG 2 cut(s) 747, 1444
MunI CAATTG 2 cut(s) 35, 1119
Mva1269I GAATGC 1 cut(s) 1534
MvaI CCWGG 2 cut(s) 747, 1444
MvnI CGCG 3 cut(s) 13, 104, 106
MwoI GCNNNNNNNGC 6 cut(s) 173, 490, 619, 726, 763, 1633
NaeI GCCGGC 1 cut(s) 1760
NgoMIV GCCGGC 1 cut(s) 1758
NlaIII CATG 8 cut(s) 445, 655, 962, 1061, 1282, 1296, 1830, 1852
NlaIV GGNNCC 4 cut(s) 631, 693, 1067, 1763
NmuCI GTSAC 7 cut(s) 178, 293, 471, 974, 1247, 1560, 1565
NspI RCATGY 2 cut(s) 1061, 1296
PaeI GCATGC 2 cut(s) 1061, 1296
PaeR7I CTCGAG 1 cut(s) 1436
PagI TCATGA 2 cut(s) 441, 958
PaqCI CACCTGC 1 cut(s) 67
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 1 cut(s) 1534
PdiI GCCGGC 1 cut(s) 1760
PdmI GAANNNNTTC 2 cut(s) 1503, 1866
PfeI GAWTC 8 cut(s) 8, 108, 904, 1174, 1537, 1677, 1788, 1867
PkrI GCNGC 9 cut(s) 15, 327, 744, 799, 859, 1275, 1339, 1342, 1774
PleI GAGTC 4 cut(s) 162, 517, 1244, 1344
PpsI GAGTC 4 cut(s) 162, 517, 1244, 1344
Ppu21I YACGTR 1 cut(s) 1229
Psp124BI GAGCTC 2 cut(s) 359, 1113
Psp6I CCWGG 2 cut(s) 745, 1442
PspFI CCCAGC 2 cut(s) 1061, 1532
PspGI CCWGG 2 cut(s) 745, 1442
PspN4I GGNNCC 4 cut(s) 631, 693, 1067, 1763
PspPI GGNCC 3 cut(s) 166, 349, 1309
PspXI VCTCGAGB 1 cut(s) 1436
PsrI GAACNNNNNNTAC 2 cut(s) 993, 1025
PstI CTGCAG 2 cut(s) 495, 945
PsuI RGATCY 2 cut(s) 1038, 1156
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
SacI GAGCTC 2 cut(s) 359, 1113
SaqAI TTAA 8 cut(s) 162, 312, 564, 879, 1044, 1077, 1236, 1972
SatI GCNGC 9 cut(s) 14, 326, 743, 798, 858, 1274, 1338, 1341, 1773
Sau96I GGNCC 3 cut(s) 166, 349, 1309
SchI GAGTC 4 cut(s) 163, 518, 1244, 1344
ScrFI CCNGG 2 cut(s) 747, 1444
SduI GDGCHC 4 cut(s) 359, 1113, 1149, 1319
SfaNI GCATC 3 cut(s) 958, 1199, 1248
SfcI CTRYAG 2 cut(s) 491, 941
Sfr274I CTCGAG 1 cut(s) 1436
SinI GGWCC 2 cut(s) 349, 1309
SlaI CTCGAG 1 cut(s) 1436
SmlI CTYRAG 4 cut(s) 203, 1217, 1328, 1436
SmoI CTYRAG 4 cut(s) 203, 1217, 1328, 1436
SnaBI TACGTA 1 cut(s) 1229
SphI GCATGC 2 cut(s) 1061, 1296
Sse9I AATT 9 cut(s) 35, 538, 549, 880, 1119, 1194, 1242, 1693, 1715
SsiI CCGC 4 cut(s) 13, 102, 1484, 1905
SspI AATATT 1 cut(s) 1323
SspMI CTAG 3 cut(s) 432, 1602, 1637
SstI GAGCTC 2 cut(s) 359, 1113
StyD4I CCNGG 2 cut(s) 745, 1442
TaaI ACNGT 2 cut(s) 310, 844
TaiI ACGT 3 cut(s) 1231, 1557, 1567
TaqI TCGA 4 cut(s) 47, 787, 1437, 1618
TasI AATT 9 cut(s) 35, 538, 549, 880, 1119, 1194, 1242, 1693, 1715
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 8 cut(s) 8, 108, 904, 1174, 1537, 1677, 1788, 1867
Tru1I TTAA 8 cut(s) 162, 312, 564, 879, 1044, 1077, 1236, 1972
Tru9I TTAA 8 cut(s) 162, 312, 564, 879, 1044, 1077, 1236, 1972
TscAI CASTG 4 cut(s) 189, 768, 979, 1054
TseFI GTSAC 7 cut(s) 178, 293, 471, 974, 1247, 1560, 1565
TseI GCWGC 8 cut(s) 325, 742, 797, 857, 1273, 1337, 1340, 1772
Tsp45I GTSAC 7 cut(s) 178, 293, 471, 974, 1247, 1560, 1565
TspDTI ATGAA 9 cut(s) 113, 458, 570, 940, 1001, 1166, 1512, 1575, 1674
TspGWI ACGGA 4 cut(s) 362, 996, 1143, 1689
TspRI CASTG 4 cut(s) 189, 768, 979, 1054
VneI GTGCAC 1 cut(s) 1315
VpaK11BI GGWCC 2 cut(s) 349, 1309
XapI RAATTY 1 cut(s) 1693
XbaI TCTAGA 1 cut(s) 1601
XceI RCATGY 2 cut(s) 1061, 1296
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 1436
XmnI GAANNNNTTC 2 cut(s) 1503, 1866
XspI CTAG 3 cut(s) 432, 1602, 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.