Rroxscaffold_3G00270200

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013673
Physical Location & Seq
Reverse (-)
62806852 .. 62815859
9008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270200.1

Sequence Viewer

Length: 1890 bp
ATGGGGCTATTGACCAATAAGTGGTCTCCTGTTACATATGCTGACAATGACACTGTCGCCCATCGCGCGATTCTCCGTACAAATTTTCCGCTTCACCAATTAACGCTCGTGATCAGGTCCTTGTCTCCATCCGACGGCCCTCCTTCTTCATCTTTACAAAATTTGAAACTGAAAGCGCAGAAAAAGGGCGGTCCTGATCATTTTCAAAGGGCGGTCAAGCCCAGCCGTTGGCACCTGCTGAGAGAGTGGAAGGATCTGCACCCTGTTGTCACCTTGATCCCCTACTTGAAAGTAACATCTTCATCCTTAGTTATGCCTACTAAGTTGGAGAGTCCCGTTAAGACGCAGATGGCTGTGGGACTTGTCAGTGGTCTGCTCAATGGTGAGTGCCGTGAGAGCACAAGAGGAATTCTTGCTCGGAGGAGGCGTGTCTTTGTGCAGACTGAGACTGGCTGTGTTTTGGGAATGGACTTGGAAAGGGGTGACAATGCCAACACAGTTAAAAGGAGGTTGCAGCTTGCCCTCAATGTACCTACTGACCACAGCTCTTTGACATTCGGGGACCGAATATTGAATAACGATCTAAGTGCCATCCGCAATCATTCCCCGCTACTGCTTGCAAGGAATGTCATGCATAGAAGCTCCTCAACTCCTTGTCTCTCACCTACTGGGAGAAATCTTCAACATTGGGATCGGAGTGGTCCTATTGAGATTCTAGGGTGTTCAAAGTACTTTGCTAGTATTGATGAACTGATCAAGGACATAGTGGTGGCTATCAAGAAGGGCGTTGAACCAATACCTGTTCATAGTGGGCTTGGTGGTGCTTACTACTTCAGGAACATCAGGGGTGAGAGTGTTGCCATTGTGAAACCAACAGATGAGGAGCCATATGCACCCAACAACCCCAAAGGCTTTGTAGGCACATCTCTTGGGCTGCCAGGCCTGAAGCATTCAGTGAGAGTTGGGGAAACAGGGTTTCGAGAAGTTGCTGCCTACCTTTTGGATGTCAATCACTATGCTAATGTGCCTCCAACTGTTCTTGTTAAGGTTACACATTCAATCTTCAATGTTAATTATGGTGTCAATGGAGACAAGATTCAGCAGAAGAAAAAGGTTAGTAAAATTGCATCTCTGCAGCAGTTCATCCGTCATGACTTTGATGCTGGTGACTATGGAACCTCAAGTTTTCCTGTAGCTGGTGTTCACAGAATAGGCATTTTAGATATTAGGATTTTTAACACTGACAGGCATGCAGGAAACATTCTGGTTAAAATGCCCGATGGGGAGTTTGGTCAGGTGGAGCTCATTCCCATAGATCATGGCCTTTGCTTGCCTGAGCACTTGGAAGATCCCTATTTTGAATGGATTCATTGGCCTCAAGCTTCAATTCCTTTCTCAGAGGACGAGCTTGATGCGATAAAGCAGGGCTTGATCCTTCTCCTCAGATCCCAAGTTTCCTCTTCAAGCAGTTTTCATATCCCTTTTGGACCTGATGGTGCTAATGGTCACTACTTACTTCCTAAGCTAGATGAGAGTTTGGACGAGGATATTGTTGACTTGGATACAAGGGCAGTCAGAGCTGAGGGAATCATTGCCCAGCCAAATTGGTATAAAACTGAAACTGTTTCGAAGTTGTCCGTGTCTCTGAAAAAGTTAAATGTGGATGAGGCAAGCCAGCTACAGAGGAAGGGTACCTTACCTGGAATCAGAAGAAGTGCAAATGACCAGTTACCTACCTCAACAAGCTTTGTGAAACTTGCTGATATGTGTGAGGAGGAATGGGTTCTGTTCCTTGAAAAGTTTAGGGAACTTCTTTACCCCGCCCTTGGCAAACGAAGGTCTACCACCATTGGACAGAAAAGGAGGCTTGGAACATCATGCACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

629

Amino Acids

69.93

Weight (kDa)

9.07

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 276 - 473 1.2e-35 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 243
Acc65I GGTACC 1 cut(s) 1691
AccB1I GGYRCC 2 cut(s) 231, 1691
AccB7I CCANNNNNTGG 2 cut(s) 21, 228
AccI GTMKAC 1 cut(s) 1841
AccII CGCG 2 cut(s) 66, 68
AciI CCGC 6 cut(s) 89, 189, 212, 595, 608, 1821
AclWI GGATC 6 cut(s) 261, 271, 699, 1343, 1426, 1440
AcsI RAATTY 3 cut(s) 82, 160, 408
AcuI CTGAAG 2 cut(s) 817, 965
AfaI GTAC 4 cut(s) 79, 531, 731, 1693
AfiI CCNNNNNNNGG 5 cut(s) 21, 134, 228, 1196, 1826
AjnI CCWGG 2 cut(s) 937, 1699
Alw21I GWGCWC 3 cut(s) 401, 1305, 1341
Alw26I GTCTC 6 cut(s) 30, 129, 440, 662, 1083, 1647
AlwI GGATC 6 cut(s) 261, 271, 699, 1343, 1426, 1440
AoxI GGCC 4 cut(s) 136, 940, 1321, 1373
ApeKI GCWGC 4 cut(s) 514, 934, 989, 1135
ApoI RAATTY 3 cut(s) 82, 160, 408
Asp700I GAANNNNTTC 2 cut(s) 1365, 1782
Asp718I GGTACC 1 cut(s) 1691
AspLEI GCGC 2 cut(s) 68, 178
AspS9I GGNCC 6 cut(s) 117, 137, 191, 562, 701, 1487
AsuHPI GGTGA 7 cut(s) 86, 262, 395, 494, 654, 860, 1178
AsuII TTCGAA 1 cut(s) 1628
AvaII GGWCC 5 cut(s) 117, 191, 562, 701, 1487
BanI GGYRCC 2 cut(s) 231, 1691
BanII GRGCYC 1 cut(s) 1305
BauI CACGAG 1 cut(s) 107
Bbv12I GWGCWC 3 cut(s) 401, 1305, 1341
BbvCI CCTCAGC 1 cut(s) 1581
BbvI GCAGC 4 cut(s) 526, 921, 976, 1147
BccI CCATC 6 cut(s) 69, 136, 343, 599, 1274, 1487
BceAI ACGGC 3 cut(s) 151, 210, 375
BcgI CGANNNNNNTGC 4 cut(s) 569, 603, 1394, 1428
BciT130I CCWGG 2 cut(s) 939, 1701
BciVI GTATCC 1 cut(s) 1555
BclI TGATCA 3 cut(s) 111, 196, 753
BcoDI GTCTC 6 cut(s) 30, 129, 440, 662, 1083, 1647
BfaI CTAG 3 cut(s) 716, 738, 1526
BfmI CTRYAG 3 cut(s) 1133, 1191, 1679
BfuAI ACCTGC 1 cut(s) 243
BfuI GTATCC 1 cut(s) 1555
BisI GCNGC 4 cut(s) 515, 935, 990, 1136
BlsI GCNGC 4 cut(s) 516, 936, 991, 1137
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 2 cut(s) 939, 1701
Bme18I GGWCC 5 cut(s) 117, 191, 562, 701, 1487
BmgT120I GGNCC 6 cut(s) 117, 137, 191, 562, 701, 1487
BmiI GGNNCC 5 cut(s) 233, 563, 885, 1177, 1693
BmrFI CCNGG 2 cut(s) 939, 1701
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 3 cut(s) 1136, 1150, 1402
BmuI ACTGGG 1 cut(s) 678
Bpu10I CCTNAGC 3 cut(s) 1335, 1521, 1581
Bpu14I TTCGAA 1 cut(s) 1628
BpuEI CTTGAG 2 cut(s) 1165, 1362
BsaBI GATNNNNATC 1 cut(s) 1008
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 1825
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 134, 228, 1196, 1826
Bse1I ACTGG 3 cut(s) 454, 673, 1726
Bse3DI GCAATG 1 cut(s) 1590
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 2 cut(s) 939, 1701
BseDI CCNNGG 1 cut(s) 1825
BseGI GGATG 6 cut(s) 128, 302, 591, 1009, 1143, 1669
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 5 cut(s) 21, 134, 228, 1196, 1826
BseMI GCAATG 1 cut(s) 1590
BseMII CTCAG 6 cut(s) 230, 435, 1326, 1410, 1456, 1572
BseNI ACTGG 3 cut(s) 454, 673, 1726
BseRI GAGGAG 5 cut(s) 436, 634, 896, 1430, 1787
BseXI GCAGC 4 cut(s) 526, 921, 976, 1147
BseYI CCCAGC 2 cut(s) 221, 1596
BsgI GTGCAG 2 cut(s) 242, 458
Bsh1236I CGCG 2 cut(s) 66, 68
BshFI GGCC 4 cut(s) 138, 942, 1323, 1375
BshNI GGYRCC 2 cut(s) 231, 1691
BsiHKAI GWGCWC 3 cut(s) 401, 1305, 1341
BslFI GGGAC 3 cut(s) 318, 372, 575
BslI CCNNNNNNNGG 5 cut(s) 21, 134, 228, 1196, 1826
BsmAI GTCTC 6 cut(s) 30, 129, 440, 662, 1083, 1647
BsmFI GGGAC 3 cut(s) 318, 372, 575
BsmI GAATGC 1 cut(s) 949
BsnI GGCC 4 cut(s) 138, 942, 1323, 1375
Bso31I GGTCTC 1 cut(s) 30
Bsp119I TTCGAA 1 cut(s) 1628
Bsp1286I GDGCHC 3 cut(s) 401, 1305, 1341
BspACI CCGC 6 cut(s) 89, 189, 212, 595, 608, 1821
BspANI GGCC 4 cut(s) 138, 942, 1323, 1375
BspCNI CTCAG 6 cut(s) 231, 436, 1327, 1409, 1455, 1573
BspFNI CGCG 2 cut(s) 66, 68
BspHI TCATGA 1 cut(s) 1150
BspLI GGNNCC 5 cut(s) 233, 563, 885, 1177, 1693
BspMAI CTGCAG 1 cut(s) 1137
BspMI ACCTGC 1 cut(s) 243
BspPI GGATC 6 cut(s) 261, 271, 699, 1343, 1426, 1440
BspT104I TTCGAA 1 cut(s) 1628
BspT107I GGYRCC 2 cut(s) 231, 1691
BspTNI GGTCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 1590
BsrI ACTGG 3 cut(s) 454, 673, 1726
BssECI CCNNGG 1 cut(s) 1825
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 1825
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 939, 1701
Bst4CI ACNGT 4 cut(s) 55, 499, 1036, 1624
Bst6I CTCTTC 1 cut(s) 1465
BstBI TTCGAA 1 cut(s) 1628
BstC8I GCNNGC 6 cut(s) 519, 618, 1251, 1331, 1672, 1676
BstF5I GGATG 6 cut(s) 128, 302, 591, 1009, 1143, 1669
BstFNI CGCG 2 cut(s) 66, 68
BstHHI GCGC 2 cut(s) 68, 178
BstMAI GTCTC 6 cut(s) 30, 129, 440, 662, 1083, 1647
BstMWI GCNNNNNNNGC 4 cut(s) 65, 396, 918, 1577
BstNI CCWGG 2 cut(s) 939, 1701
BstNSI RCATGY 1 cut(s) 1253
BstSCI CCNGG 2 cut(s) 937, 1699
BstSFI CTRYAG 3 cut(s) 1133, 1191, 1679
BstUI CGCG 2 cut(s) 66, 68
BstV1I GCAGC 4 cut(s) 526, 921, 976, 1147
BstX2I RGATCY 3 cut(s) 253, 1348, 1445
BstYI RGATCY 3 cut(s) 253, 1348, 1445
BsuI GTATCC 1 cut(s) 1555
BsuRI GGCC 4 cut(s) 138, 942, 1323, 1375
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 6 cut(s) 128, 302, 591, 1009, 1143, 1669
BtsIMutI CAGTG 4 cut(s) 51, 373, 960, 1239
BveI ACCTGC 1 cut(s) 243
Cac8I GCNNGC 6 cut(s) 519, 618, 1251, 1331, 1672, 1676
CciI TCATGA 1 cut(s) 1150
CfoI GCGC 2 cut(s) 68, 178
Cfr13I GGNCC 6 cut(s) 117, 137, 191, 562, 701, 1487
CseI GACGC 1 cut(s) 352
Csp6I GTAC 4 cut(s) 78, 530, 730, 1692
CviAII CATG 5 cut(s) 631, 1151, 1250, 1319, 1878
CviQI GTAC 4 cut(s) 78, 530, 730, 1692
Eam1104I CTCTTC 1 cut(s) 1465
EarI CTCTTC 1 cut(s) 1465
Ecl136II GAGCTC 1 cut(s) 1303
Eco130I CCWWGG 1 cut(s) 1825
Eco147I AGGCCT 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 1305
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 5 cut(s) 117, 191, 562, 701, 1487
Eco53kI GAGCTC 1 cut(s) 1303
Eco57I CTGAAG 2 cut(s) 817, 965
EcoICRI GAGCTC 1 cut(s) 1303
EcoO109I RGGNCCY 1 cut(s) 117
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 937, 1699
EcoT14I CCWWGG 1 cut(s) 1825
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 1305
ErhI CCWWGG 1 cut(s) 1825
FaeI CATG 5 cut(s) 634, 1154, 1253, 1322, 1881
FalI AAGNNNNNCTT 4 cut(s) 1412, 1444, 1679, 1711
FaqI GGGAC 3 cut(s) 318, 372, 575
FatI CATG 5 cut(s) 630, 1150, 1249, 1318, 1877
FauI CCCGC 2 cut(s) 615, 1828
FauNDI CATATG 2 cut(s) 37, 889
FbaI TGATCA 3 cut(s) 111, 196, 753
FblI GTMKAC 1 cut(s) 1841
Fnu4HI GCNGC 4 cut(s) 515, 935, 990, 1136
FokI GGATG 6 cut(s) 115, 289, 578, 1016, 1130, 1676
FriOI GRGCYC 1 cut(s) 1305
Fsp4HI GCNGC 4 cut(s) 515, 935, 990, 1136
FspBI CTAG 3 cut(s) 716, 738, 1526
GlaI GCGC 2 cut(s) 67, 177
GluI GCNGC 4 cut(s) 515, 935, 990, 1136
GsaI CCCAGC 2 cut(s) 225, 1600
HaeIII GGCC 4 cut(s) 138, 942, 1323, 1375
HgaI GACGC 1 cut(s) 352
HhaI GCGC 2 cut(s) 68, 178
Hin1II CATG 5 cut(s) 634, 1154, 1253, 1322, 1881
Hin6I GCGC 2 cut(s) 66, 176
HinP1I GCGC 2 cut(s) 66, 176
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 2 cut(s) 1380, 1744
HinfI GANTC 7 cut(s) 70, 331, 712, 1096, 1366, 1587, 1704
HphI GGTGA 7 cut(s) 86, 262, 395, 494, 654, 860, 1178
Hpy166II GTNNAC 3 cut(s) 1204, 1555, 1842
Hpy188I TCNGA 8 cut(s) 133, 420, 696, 1399, 1445, 1577, 1647, 1709
Hpy188III TCNNGA 6 cut(s) 109, 194, 778, 835, 980, 1151
Hpy8I GTNNAC 3 cut(s) 1204, 1555, 1842
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 6 cut(s) 153, 244, 775, 1445, 1681, 1830
HpyCH4III ACNGT 4 cut(s) 55, 499, 1036, 1624
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 396, 918, 1577
Hsp92II CATG 5 cut(s) 634, 1154, 1253, 1322, 1881
HspAI GCGC 2 cut(s) 66, 176
KpnI GGTACC 1 cut(s) 1695
Ksp22I TGATCA 3 cut(s) 111, 196, 753
LmnI GCTCC 3 cut(s) 647, 883, 1300
Lsp1109I GCAGC 4 cut(s) 526, 921, 976, 1147
LweI GCATC 3 cut(s) 1136, 1150, 1402
MaeI CTAG 3 cut(s) 716, 738, 1526
MaeIII GTNAC 8 cut(s) 31, 268, 292, 482, 1048, 1166, 1505, 1728
MboII GAAGA 8 cut(s) 138, 291, 671, 1054, 1117, 1358, 1452, 1722
MflI RGATCY 3 cut(s) 253, 1348, 1445
MhlI GDGCHC 3 cut(s) 401, 1305, 1341
MluCI AATT 8 cut(s) 82, 98, 160, 408, 1072, 1122, 1386, 1603
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 3 cut(s) 156, 306, 1055
Mph1103I ATGCAT 1 cut(s) 636
MroXI GAANNNNTTC 2 cut(s) 1365, 1782
MseI TTAA 8 cut(s) 101, 339, 501, 1044, 1071, 1236, 1269, 1655
MslI CAYNNNNRTG 1 cut(s) 767
MspR9I CCNGG 2 cut(s) 939, 1701
Mva1269I GAATGC 1 cut(s) 949
MvaI CCWGG 2 cut(s) 939, 1701
MvnI CGCG 2 cut(s) 66, 68
MwoI GCNNNNNNNGC 4 cut(s) 65, 396, 918, 1577
NdeI CATATG 2 cut(s) 37, 889
NlaIII CATG 5 cut(s) 634, 1154, 1253, 1322, 1881
NlaIV GGNNCC 5 cut(s) 233, 563, 885, 1177, 1693
NmuCI GTSAC 4 cut(s) 268, 482, 1166, 1505
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 1253
NspV TTCGAA 1 cut(s) 1628
PaeI GCATGC 1 cut(s) 1253
PagI TCATGA 1 cut(s) 1150
PaqCI CACCTGC 1 cut(s) 243
PceI AGGCCT 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 424
PctI GAATGC 1 cut(s) 949
PdmI GAANNNNTTC 2 cut(s) 1365, 1782
PfeI GAWTC 6 cut(s) 70, 712, 1096, 1366, 1587, 1704
PflFI GACNNNGTC 1 cut(s) 53
PflMI CCANNNNNTGG 2 cut(s) 21, 228
PkrI GCNGC 4 cut(s) 516, 936, 991, 1137
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PpuMI RGGWCCY 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 1305
Psp5II RGGWCCY 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 937, 1699
PspFI CCCAGC 2 cut(s) 221, 1596
PspGI CCWGG 2 cut(s) 937, 1699
PspN4I GGNNCC 5 cut(s) 233, 563, 885, 1177, 1693
PspPI GGNCC 6 cut(s) 117, 137, 191, 562, 701, 1487
PspPPI RGGWCCY 1 cut(s) 117
PsrI GAACNNNNNNTAC 2 cut(s) 1185, 1217
PstI CTGCAG 1 cut(s) 1137
PsuI RGATCY 3 cut(s) 253, 1348, 1445
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 4 cut(s) 79, 531, 731, 1693
RsaNI GTAC 4 cut(s) 78, 530, 730, 1692
RseI CAYNNNNRTG 1 cut(s) 767
SacI GAGCTC 1 cut(s) 1305
SaqAI TTAA 8 cut(s) 101, 339, 501, 1044, 1071, 1236, 1269, 1655
SatI GCNGC 4 cut(s) 515, 935, 990, 1136
Sau96I GGNCC 6 cut(s) 117, 137, 191, 562, 701, 1487
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 939, 1701
SduI GDGCHC 3 cut(s) 401, 1305, 1341
SfaNI GCATC 3 cut(s) 1136, 1150, 1402
SfcI CTRYAG 3 cut(s) 1133, 1191, 1679
SfuI TTCGAA 1 cut(s) 1628
SinI GGWCC 5 cut(s) 117, 191, 562, 701, 1487
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 1180, 1377
SmoI CTYRAG 2 cut(s) 1180, 1377
SphI GCATGC 1 cut(s) 1253
Sse9I AATT 8 cut(s) 82, 98, 160, 408, 1072, 1122, 1386, 1603
SseBI AGGCCT 1 cut(s) 942
SsiI CCGC 6 cut(s) 89, 189, 212, 595, 608, 1821
SspI AATATT 1 cut(s) 570
SspMI CTAG 3 cut(s) 716, 738, 1526
SstI GAGCTC 1 cut(s) 1305
StuI AGGCCT 1 cut(s) 942
StyD4I CCNGG 2 cut(s) 937, 1699
StyI CCWWGG 1 cut(s) 1825
TaaI ACNGT 4 cut(s) 55, 499, 1036, 1624
TaqI TCGA 2 cut(s) 979, 1628
TaqII GACCGA 1 cut(s) 579
TasI AATT 8 cut(s) 82, 98, 160, 408, 1072, 1122, 1386, 1603
TatI WGTACW 1 cut(s) 729
TfiI GAWTC 6 cut(s) 70, 712, 1096, 1366, 1587, 1704
Tru1I TTAA 8 cut(s) 101, 339, 501, 1044, 1071, 1236, 1269, 1655
Tru9I TTAA 8 cut(s) 101, 339, 501, 1044, 1071, 1236, 1269, 1655
TscAI CASTG 4 cut(s) 58, 373, 960, 1246
TseFI GTSAC 4 cut(s) 268, 482, 1166, 1505
TseI GCWGC 4 cut(s) 514, 934, 989, 1135
Tsp45I GTSAC 4 cut(s) 268, 482, 1166, 1505
TspDTI ATGAA 7 cut(s) 138, 291, 762, 794, 1132, 1358, 1463
TspGWI ACGGA 3 cut(s) 65, 1136, 1627
TspRI CASTG 4 cut(s) 58, 373, 960, 1246
Tth111I GACNNNGTC 1 cut(s) 53
Van91I CCANNNNNTGG 2 cut(s) 21, 228
VpaK11BI GGWCC 5 cut(s) 117, 191, 562, 701, 1487
XapI RAATTY 3 cut(s) 82, 160, 408
XceI RCATGY 1 cut(s) 1253
XmiI GTMKAC 1 cut(s) 1841
XmnI GAANNNNTTC 2 cut(s) 1365, 1782
XspI CTAG 3 cut(s) 716, 738, 1526
ZrmI AGTACT 1 cut(s) 731
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.