Rh7BG061200

Phosphatidylinositol 4-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013673
Physical Location & Seq
Forward (+)
4099063 .. 4102486
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG061200.1

Sequence Viewer

Length: 1878 bp
ATGCCTACTAAGTTGGAGAGTCCCGTTAAGACGCAGATGGCTGTGGGACTTGTCAGTGGTCTGCTCAACGGTGAGTGCCGTGAGAGCACAAGAGGAATTCTTGCTCGGAGGAGGCGTGTCTTTGTGCAGACTGAGACTGGCTGTGTTTTGGGAATGGACTTGGAAAGGGGTGACAATGCCAACACAGTTAAAAGGAGGTTGCAGCTTGCCCTCAATGTACCTACTGACCACAGCTCTTTGACATTCGGGGATCGAATATTGAATAACGATCTAAGTGCCATCCGCAATCATTCCCCGCTACTGCTTGCAAGGAATGTCATGCATAGAAGCTCCTCCACTCCTTGTCTCTCACCTACTGGGAGAAATCTTCAACATTGGGATCGGAGTGGTCCTATTGAGATTCTAGGGTGTTCAAAGTACTTTGCTAGTATTGATGAACTGATTAAGGACATAGTGGTGGCTATCAAGAAGGGCGTTGAACCAATACCTGTTCATAGTGGGCTTGGTGGTGCTTCCTACTTCAGGAACATCAGGGGTGAGAGTGTTGCCATTGTGAAACCAACAGATGAGGAGCCATATGCACCCAACAACCCCAAAGGCTTTGTAGGCACATCACTTGGGCTGCCAGGCCTGAAGCATTCAGTGAGAGTTGGGGAAACAGGGTTTCGAGAAGTTGCTGCCTACCTTTTGGATGTCAATCACTATGCTAATGTGCCTCCAACTGTTCTTGTTAAGGTGACACATTCAATCTTCAATGTTAATTATGGTGTCAATGGAGACAAGATTCAGCAGAAGAAAAAGGTTAGTAAAATTGCATCTCTGCAGCAGTTCATCCGTCATGACTTTGATGCTGGTGACTATGGAACCTCAAGTTTTCCTGTAGCTGGTGTTCACAGAATAGGCATTTTAGATATTAGGATCTTTAACACTGACAGGCATGCAGGAAACATTCTTGTTAAAATGCCCGATGGGGAGTTTGGTCAGGTGGAGCTCATTCCCATAGATCATGGCCTTTGCTTGCCTGAGCACTTGGAAGATCCCTATTTTGAATGGATTCATTGGCCTCAAGCTTCAATTCCTTTCTCAGAGGACGAGCTTGAGTATATAAAGAATCTTGATCCAATTCGTGATTCCGAGATGCTCAAGATGGAACTACCCATGATTCATGAGGCATGTCTTCGAGTTCTGGTCCTCTCCACAATATTCCTCCAGGAAGCAGCTGCTTTTGGACTTTGCCTTGCTGAGATAGGTGAAATGATGAGCAGGGAGTTTGGAAGCAAAGAGGAGAAGCCAAGTGAACTTGAGGTTGTATGCCTTGAGACTAGGAGGATCATAACAGATTCGAACATGTTAAACTTTGTGGCTGAAGAAGACAACAATCAGTGTCTGTCTGATATAGAGTGCGATAAAGCAGGGCTTGATCCTTCTCCTCAGATCCCAAGTTTCCTCTTCAAGCAGTTTTCATATCCCTTTGGACCTGATGGTGCTAATGGTCACTACTTACTTCCTAAGCTAGATGAGAGTTTGGAAGAGGATATTGTTGACTTGGATACAAGGGCAATCAAAGCTGAGGGAATCATTACCCAGCCAAATTGGTATAAAACTGAAACTGTTTCGAAGTTGTCCATGTCTCTGAAAAAGTCAAATGTGGATGAGACAAGCCAGCTACAGCAGAAGGGTACCTTACCTGGAATCAGAAGAAGTGCAAATGATCAGTTACCCACCTCATCAAGCTTTGTGAAACTTGCTGATATGTGTGAGGAGGAATGGGTTCTGTTCCTTGAAAAGTTTAGGGAACTTCTTTACCCCGCCCTTGGCAAACGAAGGTCTACCACCATTGGGCAGAAAAGGAGGCTTGGAACATCATGCACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

625

Amino Acids

69.69

Weight (kDa)

5.81

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 182 - 415 1.3e-44 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13340 FvH4_5g13340 FvH4_5g13340
malus_domestica MD14G1217200.v1.1
prunus_persica Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1 Prupe.5G210600_v2.0.a1
pyrus_communis pycom14g17990
rosa_chinensis RchiOBHm_Chr7g0183801
rosa_laevigata RLG00000005022
rosa_multiflora Rmu_sc0003610.1_g000041
rosa_roxburghii Rroxscaffold_3G00270200
rosa_rugosa Rorug06G0460500
rosa_samantha Rh7AG061200 Rh7BG061200 Rh7CG062900 Rh7DG061300
rosa_wichuraiana Rw7G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 1 cut(s) 1679
AccI GTMKAC 1 cut(s) 1829
AciI CCGC 3 cut(s) 283, 296, 1809
AclWI GGATC 8 cut(s) 258, 387, 926, 1031, 1112, 1337, 1415, 1429
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 3 cut(s) 505, 653, 1386
AfaI GTAC 3 cut(s) 219, 419, 1681
AfiI CCNNNNNNNGG 4 cut(s) 522, 884, 1814, 1839
AflIII ACRYGT 1 cut(s) 1347
AjnI CCWGG 3 cut(s) 625, 1209, 1687
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
Alw21I GWGCWC 3 cut(s) 89, 993, 1029
Alw26I GTCTC 6 cut(s) 128, 350, 771, 1313, 1635, 1649
AlwI GGATC 8 cut(s) 258, 387, 926, 1031, 1112, 1337, 1415, 1429
AlwNI CAGNNNCTG 1 cut(s) 1387
AoxI GGCC 3 cut(s) 628, 1009, 1061
ApeKI GCWGC 6 cut(s) 202, 622, 677, 823, 1217, 1220
ApoI RAATTY 1 cut(s) 96
Asp700I GAANNNNTTC 2 cut(s) 1053, 1770
Asp718I GGTACC 1 cut(s) 1679
AspS9I GGNCC 3 cut(s) 389, 1189, 1475
AsuHPI GGTGA 7 cut(s) 83, 182, 342, 548, 748, 866, 1262
AsuII TTCGAA 2 cut(s) 1343, 1616
AvaII GGWCC 3 cut(s) 389, 1189, 1475
BanI GGYRCC 1 cut(s) 1679
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 2 cut(s) 1169, 1377
Bbv12I GWGCWC 3 cut(s) 89, 993, 1029
BbvCI CCTCAGC 1 cut(s) 1569
BbvI GCAGC 6 cut(s) 214, 609, 664, 835, 1207, 1229
BccI CCATC 5 cut(s) 31, 287, 962, 1141, 1475
BceAI ACGGC 1 cut(s) 63
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 3 cut(s) 627, 1211, 1689
BciVI GTATCC 1 cut(s) 1543
BclI TGATCA 1 cut(s) 1711
BcoDI GTCTC 6 cut(s) 128, 350, 771, 1313, 1635, 1649
BfaI CTAG 4 cut(s) 404, 426, 1323, 1514
BfmI CTRYAG 3 cut(s) 821, 879, 1667
BfuI GTATCC 1 cut(s) 1543
BisI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
BlsI GCNGC 6 cut(s) 204, 624, 679, 825, 1219, 1222
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 3 cut(s) 627, 1211, 1689
Bme18I GGWCC 3 cut(s) 389, 1189, 1475
BmgT120I GGNCC 3 cut(s) 389, 1189, 1475
BmiI GGNNCC 3 cut(s) 573, 865, 1681
BmrFI CCNGG 3 cut(s) 627, 1211, 1689
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 3 cut(s) 824, 838, 1128
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 2 cut(s) 1169, 1377
BpmI CTGGAG 1 cut(s) 1193
Bpu10I CCTNAGC 3 cut(s) 1023, 1509, 1569
Bpu14I TTCGAA 2 cut(s) 1343, 1616
BpuEI CTTGAG 6 cut(s) 853, 1050, 1118, 1127, 1322, 1337
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 1 cut(s) 1813
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 4 cut(s) 522, 884, 1814, 1839
Bse1I ACTGG 2 cut(s) 142, 361
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 3 cut(s) 627, 1211, 1689
BseDI CCNNGG 1 cut(s) 1813
BseGI GGATG 4 cut(s) 279, 697, 831, 1657
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 4 cut(s) 522, 884, 1814, 1839
BseMII CTCAG 6 cut(s) 123, 1014, 1098, 1233, 1445, 1560
BseNI ACTGG 2 cut(s) 142, 361
BseRI GAGGAG 6 cut(s) 124, 322, 584, 1298, 1419, 1775
BseXI GCAGC 6 cut(s) 214, 609, 664, 835, 1207, 1229
BseYI CCCAGC 1 cut(s) 1584
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 3 cut(s) 630, 1011, 1063
BshNI GGYRCC 1 cut(s) 1679
BsiHKAI GWGCWC 3 cut(s) 89, 993, 1029
BslFI GGGAC 2 cut(s) 6, 60
BslI CCNNNNNNNGG 4 cut(s) 522, 884, 1814, 1839
BsmAI GTCTC 6 cut(s) 128, 350, 771, 1313, 1635, 1649
BsmFI GGGAC 2 cut(s) 6, 60
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 3 cut(s) 630, 1011, 1063
Bsp119I TTCGAA 2 cut(s) 1343, 1616
Bsp1286I GDGCHC 3 cut(s) 89, 993, 1029
BspACI CCGC 3 cut(s) 283, 296, 1809
BspANI GGCC 3 cut(s) 630, 1011, 1063
BspCNI CTCAG 6 cut(s) 124, 1015, 1097, 1234, 1444, 1561
BspHI TCATGA 2 cut(s) 838, 1165
BspLI GGNNCC 3 cut(s) 573, 865, 1681
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 8 cut(s) 258, 387, 926, 1031, 1112, 1337, 1415, 1429
BspT104I TTCGAA 2 cut(s) 1343, 1616
BspT107I GGYRCC 1 cut(s) 1679
BsrI ACTGG 2 cut(s) 142, 361
BssECI CCNNGG 1 cut(s) 1813
BssT1I CCWWGG 1 cut(s) 1813
Bst2UI CCWGG 3 cut(s) 627, 1211, 1689
Bst4CI ACNGT 4 cut(s) 71, 187, 724, 1612
Bst6I CTCTTC 2 cut(s) 1454, 1524
BstBI TTCGAA 2 cut(s) 1343, 1616
BstC8I GCNNGC 5 cut(s) 207, 306, 939, 1019, 1664
BstDEI CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1242, 1431, 1509, 1569
BstENI CCTNNNNNAGG 1 cut(s) 520
BstF5I GGATG 4 cut(s) 279, 697, 831, 1657
BstMAI GTCTC 6 cut(s) 128, 350, 771, 1313, 1635, 1649
BstMWI GCNNNNNNNGC 3 cut(s) 84, 606, 1565
BstNI CCWGG 3 cut(s) 627, 1211, 1689
BstNSI RCATGY 3 cut(s) 941, 1176, 1351
BstSCI CCNGG 3 cut(s) 625, 1209, 1687
BstSFI CTRYAG 3 cut(s) 821, 879, 1667
BstV1I GCAGC 6 cut(s) 214, 609, 664, 835, 1207, 1229
BstV2I GAAGAC 2 cut(s) 1169, 1377
BstX2I RGATCY 3 cut(s) 918, 1036, 1434
BstYI RGATCY 3 cut(s) 918, 1036, 1434
BsuI GTATCC 1 cut(s) 1543
BsuRI GGCC 3 cut(s) 630, 1011, 1063
BtsCI GGATG 4 cut(s) 279, 697, 831, 1657
BtsIMutI CAGTG 4 cut(s) 61, 648, 927, 1388
Cac8I GCNNGC 5 cut(s) 207, 306, 939, 1019, 1664
CaiI CAGNNNCTG 1 cut(s) 1387
CciI TCATGA 2 cut(s) 838, 1165
Cfr13I GGNCC 3 cut(s) 389, 1189, 1475
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 218, 418, 1680
CviQI GTAC 3 cut(s) 218, 418, 1680
DdeI CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1242, 1431, 1509, 1569
Eam1104I CTCTTC 2 cut(s) 1454, 1524
EarI CTCTTC 2 cut(s) 1454, 1524
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 1813
Eco147I AGGCCT 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 3 cut(s) 389, 1189, 1475
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 3 cut(s) 505, 653, 1386
EcoICRI GAGCTC 1 cut(s) 991
EcoNI CCTNNNNNAGG 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 3 cut(s) 625, 1209, 1687
EcoT14I CCWWGG 1 cut(s) 1813
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 1813
FalI AAGNNNNNCTT 4 cut(s) 1401, 1433, 1667, 1699
FaqI GGGAC 2 cut(s) 6, 60
FauI CCCGC 2 cut(s) 303, 1816
FauNDI CATATG 1 cut(s) 577
FbaI TGATCA 1 cut(s) 1711
FblI GTMKAC 1 cut(s) 1829
Fnu4HI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
FokI GGATG 4 cut(s) 266, 704, 818, 1664
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
FspBI CTAG 4 cut(s) 404, 426, 1323, 1514
GluI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
GsaI CCCAGC 1 cut(s) 1588
GsuI CTGGAG 1 cut(s) 1193
HaeIII GGCC 3 cut(s) 630, 1011, 1063
HgaI GACGC 1 cut(s) 40
HincII GTYRAC 1 cut(s) 1543
HindII GTYRAC 1 cut(s) 1543
HindIII AAGCTT 2 cut(s) 1068, 1732
HphI GGTGA 7 cut(s) 83, 182, 342, 548, 748, 866, 1262
Hpy166II GTNNAC 4 cut(s) 892, 1298, 1543, 1830
Hpy188I TCNGA 8 cut(s) 108, 384, 1087, 1135, 1393, 1434, 1635, 1697
Hpy188III TCNNGA 8 cut(s) 466, 523, 668, 839, 1115, 1127, 1144, 1166
Hpy8I GTNNAC 4 cut(s) 892, 1298, 1543, 1830
HpyAV CCTTC 4 cut(s) 463, 1434, 1669, 1818
HpyCH4III ACNGT 4 cut(s) 71, 187, 724, 1612
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 606, 1565
HpyF3I CTNAG 9 cut(s) 9, 132, 272, 1023, 1084, 1242, 1431, 1509, 1569
KpnI GGTACC 1 cut(s) 1683
Ksp22I TGATCA 1 cut(s) 1711
LmnI GCTCC 3 cut(s) 335, 571, 988
Lsp1109I GCAGC 6 cut(s) 214, 609, 664, 835, 1207, 1229
LweI GCATC 3 cut(s) 824, 838, 1128
MaeI CTAG 4 cut(s) 404, 426, 1323, 1514
MaeIII GTNAC 5 cut(s) 170, 736, 854, 1493, 1716
MflI RGATCY 3 cut(s) 918, 1036, 1434
MhlI GDGCHC 3 cut(s) 89, 993, 1029
MluCI AATT 6 cut(s) 96, 760, 810, 1074, 1122, 1591
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 743
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 2 cut(s) 1053, 1770
MseI TTAA 8 cut(s) 27, 189, 444, 732, 759, 924, 957, 1352
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 1220
MspR9I CCNGG 3 cut(s) 627, 1211, 1689
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 3 cut(s) 627, 1211, 1689
MwoI GCNNNNNNNGC 3 cut(s) 84, 606, 1565
NdeI CATATG 1 cut(s) 577
NlaIV GGNNCC 3 cut(s) 573, 865, 1681
NmuCI GTSAC 4 cut(s) 170, 736, 854, 1493
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 3 cut(s) 941, 1176, 1351
NspV TTCGAA 2 cut(s) 1343, 1616
PaeI GCATGC 1 cut(s) 941
PagI TCATGA 2 cut(s) 838, 1165
PceI AGGCCT 1 cut(s) 630
PciI ACATGT 1 cut(s) 1347
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 2 cut(s) 1053, 1770
PfeI GAWTC 9 cut(s) 400, 784, 1054, 1111, 1130, 1162, 1340, 1575, 1692
PfoI TCCNGGA 1 cut(s) 1209
PkrI GCNGC 6 cut(s) 204, 624, 679, 825, 1219, 1222
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PscI ACATGT 1 cut(s) 1347
Psp124BI GAGCTC 1 cut(s) 993
Psp6I CCWGG 3 cut(s) 625, 1209, 1687
PspFI CCCAGC 1 cut(s) 1584
PspGI CCWGG 3 cut(s) 625, 1209, 1687
PspN4I GGNNCC 3 cut(s) 573, 865, 1681
PspPI GGNCC 3 cut(s) 389, 1189, 1475
PsrI GAACNNNNNNTAC 2 cut(s) 873, 905
PstI CTGCAG 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 1387
PsuI RGATCY 3 cut(s) 918, 1036, 1434
PvuII CAGCTG 1 cut(s) 1220
RsaI GTAC 3 cut(s) 219, 419, 1681
RsaNI GTAC 3 cut(s) 218, 418, 1680
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 8 cut(s) 27, 189, 444, 732, 759, 924, 957, 1352
SatI GCNGC 6 cut(s) 203, 623, 678, 824, 1218, 1221
Sau96I GGNCC 3 cut(s) 389, 1189, 1475
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 3 cut(s) 627, 1211, 1689
SduI GDGCHC 3 cut(s) 89, 993, 1029
SfaNI GCATC 3 cut(s) 824, 838, 1128
SfcI CTRYAG 3 cut(s) 821, 879, 1667
SfuI TTCGAA 2 cut(s) 1343, 1616
SinI GGWCC 3 cut(s) 389, 1189, 1475
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 6 cut(s) 868, 1065, 1097, 1142, 1301, 1316
SmoI CTYRAG 6 cut(s) 868, 1065, 1097, 1142, 1301, 1316
SphI GCATGC 1 cut(s) 941
Sse9I AATT 6 cut(s) 96, 760, 810, 1074, 1122, 1591
SseBI AGGCCT 1 cut(s) 630
SsiI CCGC 3 cut(s) 283, 296, 1809
SspI AATATT 2 cut(s) 258, 1203
SspMI CTAG 4 cut(s) 404, 426, 1323, 1514
SstI GAGCTC 1 cut(s) 993
StuI AGGCCT 1 cut(s) 630
StyD4I CCNGG 3 cut(s) 625, 1209, 1687
StyI CCWWGG 1 cut(s) 1813
TaaI ACNGT 4 cut(s) 71, 187, 724, 1612
TaqI TCGA 5 cut(s) 253, 667, 1180, 1343, 1616
TasI AATT 6 cut(s) 96, 760, 810, 1074, 1122, 1591
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 9 cut(s) 400, 784, 1054, 1111, 1130, 1162, 1340, 1575, 1692
Tru1I TTAA 8 cut(s) 27, 189, 444, 732, 759, 924, 957, 1352
Tru9I TTAA 8 cut(s) 27, 189, 444, 732, 759, 924, 957, 1352
TscAI CASTG 4 cut(s) 61, 648, 934, 1388
TseFI GTSAC 4 cut(s) 170, 736, 854, 1493
TseI GCWGC 6 cut(s) 202, 622, 677, 823, 1217, 1220
Tsp45I GTSAC 4 cut(s) 170, 736, 854, 1493
TspDTI ATGAA 6 cut(s) 450, 482, 820, 1046, 1154, 1452
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 4 cut(s) 61, 648, 934, 1388
VpaK11BI GGWCC 3 cut(s) 389, 1189, 1475
XagI CCTNNNNNAGG 1 cut(s) 520
XapI RAATTY 1 cut(s) 96
XceI RCATGY 3 cut(s) 941, 1176, 1351
XmiI GTMKAC 1 cut(s) 1829
XmnI GAANNNNTTC 2 cut(s) 1053, 1770
XspI CTAG 4 cut(s) 404, 426, 1323, 1514
ZrmI AGTACT 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.