Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0017144

Physical Location & Seq

Genomic Coordinates Forward (+)

8285056 .. 8286951

Gene Structure 1896 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14610.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1896 bp

ATGCTGAAGCTCTCCCGTCCTTTTCTCCGCGCTTTCGGTACATGCTCCTCTTCTCCTAATTCGCATGCCGATTCGATACTAAATTGCAGGAGTCCTGCAGAAGCACTGGAAATCTTCAATTCCGCAACGAAACAAGTCGGCGCGAGAAAGGATTTTCAGCTGTATTCAGCGATTACGCATGTGTTGGTGAGTGCCAAGCTGTATGTTAAAGCCAGGTTGTTGATAAAAGAGCTCATACAGGATCTTCAGAAGTCCTGTAAGTCTCGCCGTGCCTGTGAGTTGGTTTACAATGCGCTTAGCGGTTTGGAAAGGTCTAGAGTGTCTCGGAATGTGTTTGGAGTTTTGATCAATGCGTTATCTGAGATGGGTCTTGTGGAAGAGGCTTTGCGAGTGTATAGTAATATGGGGGTGTTGCCTGCAACTCAGGCTTGTAATGCGCTCTTGGATGGATTGGTGAAGAAGGGTCAGCTTGATTCGATGTGGGAGCTTTATAATGATATGGAGTCTCGAGGATTGTCACCTAGTTGTGTTACATATGGTGTGTTGATTAAGGGTTGTTGCAGAAAAGGTGATCTGTTGAATGCCCGTAAGGTGTTCGATGAAATGCCTGAGAGAGGGATTGAACGGACAGTTGTGGTGTATACTTCTCTTATTCGTGGCATGTGCACTGAGGGTAGAATGGAGGAAGCTGATAGCGTTTTTCAGACGATGAGGAAAGATGGTGTGATCCCAAATTTGTGGACTTACAATTGTCTGATGGATGGGTATTCCAAAGTGGCTGATGTTAGACAAGCTCTTGTGTTGTACTCGAACTTGAGAGGTGATGGCTTGCAGCCGAATGTTGTCACGTTTGGTATCTTAGTTGATGCATTTTGCAAAGTAGGTGCACTCATGGCTGCGAGGAGCGTTGTAGGGTGGATGGTTAAGTTTGGTGTTGCGCCTAATATGTTTGTGTACAATGCTTTGATTTATGGTTATCGTAAGGTCGAAGATGTTTTGCAGGCAGTGAATTTGACATTGGAGATGGAAAAGTATGGTATTCTTCCGGATGTGGTCACTTACAGCATACTAATTACGGGTCTATGTTCCATTCATAGAGTGGAAGATGCATATCAGTTGTTGAAAAACATAAGGAAGGGAGTTGCTGCAAATGCTGTTACGTACAATTCACTAATCGATGGGTATTGTAAAGATCGCAATATGGAGAAGGCTTTGGAGGTGTGTTCTGAAATGACCAAAATTGGTATAGAACCGAATGTCATCACCTTCTCTACTTTGATCGATGCTTATTGCAAGATGAGGAACATGGAAGCTGCTATGGGTTTGTACTCGGAAATGTTAGTTAAAGGCCTTTTGCCTGATGTAGTAGCTTACACAGCTTTGATAGATGGCCATTTTAAAACCGGGAACGTGAAAGAGGCTTATCGGCTGAGTAAAGAGATGTTGGAGGCAGGCCTCACCCCAAATGTTTTCACTCTTAGTTGCTTGGTAGATGGCCTTTGTAAAAACGGAAAGACTTCTGATGCAATCAACCACTTTTCAGAGATAACTAAAGTCGATTCCAACACAGATGTTACCAAGGATATGGATGGCTACTTCTGTTCCCCAAATTATGTGACTTATACGGCTTTAATTCAGGGTCTGTCTGTAGACAGCCGGATTTTCGAGGCAACTAAGTTCTTTTTGGATATGAGAGTCAGTGGTTTGAGACCAGATGCATTCACTTACATTGTAATGTTGAAGGGGCACTTCCTAGCGAAGCATGAGCTTGGCATCATGATGCTGCATGCAGATATGACGAAGATGGGTATTATTCCCAGTGCAGACATAAGTCCAGTTTTATGTAGGGCATACCAAGAAATCGGTGACTTAAAATCAGCAGCGAAGATATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

632

Amino Acids

69.98

Weight (kDa)

8.13

Isoelectric Point (pI)

31.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	141 - 188	2.4e-16	PPR repeat family
PPR_long	PF17177	159 - 295	1.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	170 - 202	8.1e-11	PPR repeat
PPR_2	PF13041	177 - 222	1.4e-12	PPR repeat family
PPR	PF01535	177 - 207	2.4e-08	PPR repeat
PPR_3	PF13812	197 - 256	1.2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	205 - 238	1.8e-07	PPR repeat
PPR_2	PF13041	210 - 258	2e-15	PPR repeat family
PPR	PF01535	212 - 242	2.5e-07	PPR repeat
PPR_2	PF13041	244 - 293	7.7e-13	PPR repeat family
PPR_3	PF13812	269 - 324	5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	275 - 304	1.6e-06	PPR repeat
PPR_2	PF13041	314 - 362	3.9e-14	PPR repeat family
PPR_1	PF12854	346 - 375	1.5e-09	PPR repeat
MRP-S27	PF10037	352 - 449	3.9e-06	Mitochondrial 28S ribosomal protein S27
PPR_long	PF17177	370 - 481	9e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	378 - 432	1e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	379 - 412	2.5e-11	PPR repeat
PPR_2	PF13041	384 - 432	6.2e-19	PPR repeat family
PPR	PF01535	386 - 416	1e-09	PPR repeat
PPR_1	PF12854	415 - 446	7.9e-10	PPR repeat
PPR	PF01535	421 - 451	1.9e-07	PPR repeat
PPR_2	PF13041	421 - 464	2.2e-12	PPR repeat family
PPR_3	PF13812	443 - 497	5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	449 - 481	1.3e-08	PPR repeat
PPR_2	PF13041	453 - 502	2.4e-16	PPR repeat family
PPR_1	PF12854	485 - 515	3.2e-08	PPR repeat
PPR_2	PF13041	536 - 583	1.4e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61400
fragaria_vesca	FvH4_5g14610
prunus_persica	Prupe.5G197300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0181881
rosa_laevigata	RLG00000005166
rosa_multiflora	Rmu_co8156836.1_g000001
rosa_roxburghii	Rroxscaffold_3G00271670
rosa_rugosa	Rorug06G0444800
rosa_samantha	Rh7AG047000 Rh7BG046600 Rh7CG047100 Rh7DG046200
rosa_wichuraiana	Rw7G003890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	492
AccI	GTMKAC	2 cut(s)	641, 1650
AccII	CGCG	2 cut(s)	30, 143
AccIII	TCCGGA	1 cut(s)	1045
AciI	CCGC	3 cut(s)	28, 123, 300
AclWI	GGATC	2 cut(s)	249, 721
AcoI	YGGCCR	1 cut(s)	1390
AcsI	RAATTY	2 cut(s)	733, 1009
AcuI	CTGAAG	2 cut(s)	26, 230
AfaI	GTAC	5 cut(s)	40, 806, 956, 1163, 1328
AfiI	CCNNNNNNNGG	1 cut(s)	614
AgsI	TTSAA	5 cut(s)	118, 580, 623, 1123, 1741
AjnI	CCWGG	1 cut(s)	212
AjuI	GAANNNNNNNTTGG	2 cut(s)	1001, 1033
Alw21I	GWGCWC	3 cut(s)	234, 668, 889
Alw26I	GTCTC	4 cut(s)	267, 327, 510, 1702
Alw44I	GTGCAC	2 cut(s)	664, 885
AlwI	GGATC	2 cut(s)	249, 721
AlwNI	CAGNNNCTG	1 cut(s)	1642
Ama87I	CYCGRG	1 cut(s)	507
Aor13HI	TCCGGA	1 cut(s)	1045
AoxI	GGCC	4 cut(s)	1348, 1390, 1453, 1495
ApaLI	GTGCAC	2 cut(s)	664, 885
ApeKI	GCWGC	6 cut(s)	832, 896, 1145, 1313, 1783, 1880
ApoI	RAATTY	2 cut(s)	733, 1009
Asp700I	GAANNNNTTC	2 cut(s)	1516, 1889
AspLEI	GCGC	5 cut(s)	32, 143, 295, 439, 940
AsuC2I	CCSGG	1 cut(s)	1405
AsuHPI	GGTGA	8 cut(s)	199, 466, 510, 581, 833, 1255, 1450, 1877
AvaI	CYCGRG	1 cut(s)	507
BaeGI	GKGCMC	3 cut(s)	668, 889, 1749
BalI	TGGCCA	1 cut(s)	1392
BanII	GRGCYC	1 cut(s)	234
Bbv12I	GWGCWC	3 cut(s)	234, 668, 889
BbvI	GCAGC	6 cut(s)	844, 883, 1132, 1300, 1770, 1892
BceAI	ACGGC	2 cut(s)	252, 1641
BciT130I	CCWGG	1 cut(s)	214
BclI	TGATCA	1 cut(s)	345
BcnI	CCSGG	1 cut(s)	1405
BcoDI	GTCTC	4 cut(s)	267, 327, 510, 1702
BfaI	CTAG	3 cut(s)	315, 522, 1754
BfmI	CTRYAG	2 cut(s)	96, 1647
BisI	GCNGC	6 cut(s)	833, 897, 1146, 1314, 1784, 1881
BlpI	GCTNAGC	1 cut(s)	296
BlsI	GCNGC	6 cut(s)	834, 898, 1147, 1315, 1785, 1882
Bme1390I	CCNGG	2 cut(s)	214, 1405
BmeT110I	CYCGRG	1 cut(s)	507
BmrFI	CCNGG	2 cut(s)	214, 1405
BmrI	ACTGGG	1 cut(s)	1812
BmsI	GCATC	7 cut(s)	856, 1096, 1273, 1513, 1705, 1770, 1782
BmuI	ACTGGG	1 cut(s)	1812
BoxI	GACNNNNGTC	1 cut(s)	1830
Bpu1102I	GCTNAGC	1 cut(s)	296
BpuEI	CTTGAG	1 cut(s)	835
BpuMI	CCSGG	1 cut(s)	1405
Bsa29I	ATCGAT	2 cut(s)	1176, 1281
BsaAI	YACGTR	1 cut(s)	1161
BsaBI	GATNNNNATC	1 cut(s)	1110
BsaI	GGTCTC	1 cut(s)	1702
BsaJI	CCNNGG	1 cut(s)	1578
BsaWI	WCCGGW	1 cut(s)	1045
Bsc4I	CCNNNNNNNGG	1 cut(s)	614
Bse1I	ACTGG	3 cut(s)	111, 1818, 1835
Bse8I	GATNNNNATC	1 cut(s)	1110
BseAI	TCCGGA	1 cut(s)	1045
BseBI	CCWGG	1 cut(s)	214
BseCI	ATCGAT	2 cut(s)	1176, 1281
BseDI	CCNNGG	1 cut(s)	1578
BseGI	GGATG	5 cut(s)	451, 766, 924, 1054, 1594
BseJI	GATNNNNATC	1 cut(s)	1110
BseLI	CCNNNNNNNGG	1 cut(s)	614
BseMII	CTCAG	5 cut(s)	351, 437, 600, 660, 1421
BseNI	ACTGG	3 cut(s)	111, 1818, 1835
BseRI	GAGGAG	2 cut(s)	37, 916
BseSI	GKGCMC	3 cut(s)	668, 889, 1749
BseXI	GCAGC	6 cut(s)	844, 883, 1132, 1300, 1770, 1892
BsgI	GTGCAG	1 cut(s)	1842
Bsh1236I	CGCG	2 cut(s)	30, 143
BshFI	GGCC	4 cut(s)	1350, 1392, 1455, 1497
BshVI	ATCGAT	2 cut(s)	1176, 1281
BsiHKAI	GWGCWC	3 cut(s)	234, 668, 889
BsiHKCI	CYCGRG	1 cut(s)	507
BsiSI	CCGG	3 cut(s)	1046, 1404, 1657
BslI	CCNNNNNNNGG	1 cut(s)	614
BsmAI	GTCTC	4 cut(s)	267, 327, 510, 1702
BsmI	GAATGC	2 cut(s)	586, 1718
BsnI	GGCC	4 cut(s)	1350, 1392, 1455, 1497
Bso31I	GGTCTC	1 cut(s)	1702
BsoBI	CYCGRG	1 cut(s)	507
Bsp1286I	GDGCHC	4 cut(s)	234, 668, 889, 1749
Bsp13I	TCCGGA	1 cut(s)	1045
Bsp1407I	TGTACA	1 cut(s)	954
Bsp143I	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
Bsp1720I	GCTNAGC	1 cut(s)	296
BspACI	CCGC	3 cut(s)	28, 123, 300
BspANI	GGCC	4 cut(s)	1350, 1392, 1455, 1497
BspCNI	CTCAG	5 cut(s)	352, 436, 601, 661, 1422
BspDI	ATCGAT	2 cut(s)	1176, 1281
BspEI	TCCGGA	1 cut(s)	1045
BspFNI	CGCG	2 cut(s)	30, 143
BspHI	TCATGA	1 cut(s)	1776
BspMAI	CTGCAG	1 cut(s)	100
BspPI	GGATC	2 cut(s)	249, 721
BspTNI	GGTCTC	1 cut(s)	1702
BsrGI	TGTACA	1 cut(s)	954
BsrI	ACTGG	3 cut(s)	111, 1818, 1835
BssECI	CCNNGG	1 cut(s)	1578
BssMI	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
BssNAI	GTATAC	1 cut(s)	642
BssT1I	CCWWGG	1 cut(s)	1578
Bst1107I	GTATAC	1 cut(s)	642
Bst2UI	CCWGG	1 cut(s)	214
Bst4CI	ACNGT	1 cut(s)	631
Bst6I	CTCTTC	2 cut(s)	55, 372
BstAUI	TGTACA	1 cut(s)	954
BstBAI	YACGTR	1 cut(s)	1161
BstC8I	GCNNGC	6 cut(s)	66, 417, 830, 1002, 1453, 1788
BstDEI	CTNAG	9 cut(s)	296, 360, 423, 609, 669, 859, 1430, 1478, 1674
BstENI	CCTNNNNNAGG	1 cut(s)	612
BstF5I	GGATG	5 cut(s)	451, 766, 924, 1054, 1594
BstFNI	CGCG	2 cut(s)	30, 143
BstHHI	GCGC	5 cut(s)	32, 143, 295, 439, 940
BstKTI	GATC	6 cut(s)	244, 348, 574, 729, 1195, 1281
BstMAI	GTCTC	4 cut(s)	267, 327, 510, 1702
BstMBI	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
BstMWI	GCNNNNNNNGC	5 cut(s)	425, 434, 893, 1151, 1376
BstNI	CCWGG	1 cut(s)	214
BstNSI	RCATGY	5 cut(s)	45, 68, 182, 664, 1790
BstPAI	GACNNNNGTC	1 cut(s)	1830
BstSCI	CCNGG	2 cut(s)	212, 1403
BstSFI	CTRYAG	2 cut(s)	96, 1647
BstSLI	GKGCMC	3 cut(s)	668, 889, 1749
BstSNI	TACGTA	1 cut(s)	1161
BstUI	CGCG	2 cut(s)	30, 143
BstV1I	GCAGC	6 cut(s)	844, 883, 1132, 1300, 1770, 1892
BstX2I	RGATCY	1 cut(s)	241
BstXI	CCANNNNNNTGG	2 cut(s)	738, 1585
BstYI	RGATCY	1 cut(s)	241
BstZ17I	GTATAC	1 cut(s)	642
Bsu15I	ATCGAT	2 cut(s)	1176, 1281
BsuRI	GGCC	4 cut(s)	1350, 1392, 1455, 1497
BsuTUI	ATCGAT	2 cut(s)	1176, 1281
BtsCI	GGATG	5 cut(s)	451, 766, 924, 1054, 1594
BtsI	GCAGTG	1 cut(s)	1011
BtsIMutI	CAGTG	5 cut(s)	104, 666, 1011, 1705, 1825
Cac8I	GCNNGC	6 cut(s)	66, 417, 830, 1002, 1453, 1788
CaiI	CAGNNNCTG	1 cut(s)	1642
CciI	TCATGA	1 cut(s)	1776
CfoI	GCGC	5 cut(s)	32, 143, 295, 439, 940
ClaI	ATCGAT	2 cut(s)	1176, 1281
Csp6I	GTAC	5 cut(s)	39, 805, 955, 1162, 1327
CviAII	CATG	9 cut(s)	42, 65, 179, 661, 892, 1306, 1763, 1777, 1787
CviQI	GTAC	5 cut(s)	39, 805, 955, 1162, 1327
DdeI	CTNAG	9 cut(s)	296, 360, 423, 609, 669, 859, 1430, 1478, 1674
DpnI	GATC	6 cut(s)	243, 347, 573, 728, 1194, 1280
DpnII	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
DraI	TTTAAA	1 cut(s)	1399
EaeI	YGGCCR	1 cut(s)	1390
Eam1104I	CTCTTC	2 cut(s)	55, 372
EarI	CTCTTC	2 cut(s)	55, 372
Ecl136II	GAGCTC	1 cut(s)	232
Eco105I	TACGTA	1 cut(s)	1161
Eco130I	CCWWGG	1 cut(s)	1578
Eco147I	AGGCCT	2 cut(s)	1350, 1455
Eco24I	GRGCYC	1 cut(s)	234
Eco31I	GGTCTC	1 cut(s)	1702
Eco53kI	GAGCTC	1 cut(s)	232
Eco57I	CTGAAG	2 cut(s)	26, 230
Eco88I	CYCGRG	1 cut(s)	507
EcoICRI	GAGCTC	1 cut(s)	232
EcoNI	CCTNNNNNAGG	1 cut(s)	612
EcoRII	CCWGG	1 cut(s)	212
EcoT14I	CCWWGG	1 cut(s)	1578
EcoT22I	ATGCAT	3 cut(s)	871, 1111, 1720
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	1578
FaeI	CATG	9 cut(s)	45, 68, 182, 664, 895, 1309, 1766, 1780, 1790
FalI	AAGNNNNNCTT	2 cut(s)	1733, 1765
FatI	CATG	9 cut(s)	41, 64, 178, 660, 891, 1305, 1762, 1776, 1786
FauNDI	CATATG	1 cut(s)	535
FbaI	TGATCA	1 cut(s)	345
FblI	GTMKAC	2 cut(s)	641, 1650
Fnu4HI	GCNGC	6 cut(s)	833, 897, 1146, 1314, 1784, 1881
FokI	GGATG	5 cut(s)	458, 773, 931, 1061, 1601
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	6 cut(s)	833, 897, 1146, 1314, 1784, 1881
FspBI	CTAG	3 cut(s)	315, 522, 1754
GlaI	GCGC	5 cut(s)	31, 142, 294, 438, 939
GluI	GCNGC	6 cut(s)	833, 897, 1146, 1314, 1784, 1881
HaeIII	GGCC	4 cut(s)	1350, 1392, 1455, 1497
HapII	CCGG	3 cut(s)	1046, 1404, 1657
HhaI	GCGC	5 cut(s)	32, 143, 295, 439, 940
Hin1II	CATG	9 cut(s)	45, 68, 182, 664, 895, 1309, 1766, 1780, 1790
Hin6I	GCGC	5 cut(s)	30, 141, 293, 437, 938
HinP1I	GCGC	5 cut(s)	30, 141, 293, 437, 938
HinfI	GANTC	6 cut(s)	71, 91, 473, 503, 1559, 1695
HpaII	CCGG	3 cut(s)	1046, 1404, 1657
HphI	GGTGA	8 cut(s)	199, 466, 510, 581, 833, 1255, 1450, 1877
Hpy166II	GTNNAC	7 cut(s)	286, 642, 666, 741, 887, 955, 1651
Hpy188III	TCNNGA	4 cut(s)	315, 507, 1046, 1777
Hpy8I	GTNNAC	7 cut(s)	286, 642, 666, 741, 887, 955, 1651
HpyAV	CCTTC	5 cut(s)	454, 1129, 1201, 1276, 1735
HpyCH4III	ACNGT	1 cut(s)	631
HpyCH4IV	ACGT	3 cut(s)	848, 1160, 1410
HpyF10VI	GCNNNNNNNGC	5 cut(s)	425, 434, 893, 1151, 1376
HpyF3I	CTNAG	9 cut(s)	296, 360, 423, 609, 669, 859, 1430, 1478, 1674
HpySE526I	ACGT	3 cut(s)	848, 1160, 1410
Hsp92II	CATG	9 cut(s)	45, 68, 182, 664, 895, 1309, 1766, 1780, 1790
HspAI	GCGC	5 cut(s)	30, 141, 293, 437, 938
Kpn2I	TCCGGA	1 cut(s)	1045
Ksp22I	TGATCA	1 cut(s)	345
Kzo9I	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
LmnI	GCTCC	3 cut(s)	50, 484, 903
Lsp1109I	GCAGC	6 cut(s)	844, 883, 1132, 1300, 1770, 1892
LweI	GCATC	7 cut(s)	856, 1096, 1273, 1513, 1705, 1770, 1782
MaeI	CTAG	3 cut(s)	315, 522, 1754
MaeII	ACGT	3 cut(s)	848, 1160, 1410
MaeIII	GTNAC	8 cut(s)	516, 529, 844, 1054, 1156, 1573, 1615, 1865
MalI	GATC	6 cut(s)	243, 347, 573, 728, 1194, 1280
MboI	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
MboII	GAAGA	9 cut(s)	42, 106, 236, 389, 469, 1001, 1034, 1115, 1813
MfeI	CAATTG	1 cut(s)	748
MflI	RGATCY	1 cut(s)	241
MhlI	GDGCHC	4 cut(s)	234, 668, 889, 1749
MlsI	TGGCCA	1 cut(s)	1392
MluNI	TGGCCA	1 cut(s)	1392
MlyI	GAGTC	3 cut(s)	100, 512, 1704
MmeI	TCCRAC	2 cut(s)	1425, 1587
Mox20I	TGGCCA	1 cut(s)	1392
Mph1103I	ATGCAT	3 cut(s)	871, 1111, 1720
MroI	TCCGGA	1 cut(s)	1045
MroXI	GAANNNNTTC	2 cut(s)	1516, 1889
MscI	TGGCCA	1 cut(s)	1392
MseI	TTAA	7 cut(s)	207, 549, 924, 1344, 1398, 1631, 1871
MslI	CAYNNNNRTG	2 cut(s)	1733, 1778
Msp20I	TGGCCA	1 cut(s)	1392
MspA1I	CMGCKG	1 cut(s)	160
MspI	CCGG	3 cut(s)	1046, 1404, 1657
MspR9I	CCNGG	2 cut(s)	214, 1405
MunI	CAATTG	1 cut(s)	748
Mva1269I	GAATGC	2 cut(s)	586, 1718
MvaI	CCWGG	1 cut(s)	214
MvnI	CGCG	2 cut(s)	30, 143
MwoI	GCNNNNNNNGC	5 cut(s)	425, 434, 893, 1151, 1376
NciI	CCSGG	1 cut(s)	1405
NdeI	CATATG	1 cut(s)	535
NdeII	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
NlaIII	CATG	9 cut(s)	45, 68, 182, 664, 895, 1309, 1766, 1780, 1790
NmuCI	GTSAC	5 cut(s)	516, 844, 1054, 1615, 1865
NsiI	ATGCAT	3 cut(s)	871, 1111, 1720
NspI	RCATGY	5 cut(s)	45, 68, 182, 664, 1790
PaeI	GCATGC	2 cut(s)	68, 1790
PaeR7I	CTCGAG	1 cut(s)	507
PagI	TCATGA	1 cut(s)	1776
PceI	AGGCCT	2 cut(s)	1350, 1455
PctI	GAATGC	2 cut(s)	586, 1718
PdmI	GAANNNNTTC	2 cut(s)	1516, 1889
PfeI	GAWTC	3 cut(s)	71, 473, 1559
PkrI	GCNGC	6 cut(s)	834, 898, 1147, 1315, 1785, 1882
PleI	GAGTC	3 cut(s)	99, 511, 1703
PpsI	GAGTC	3 cut(s)	99, 511, 1703
Ppu21I	YACGTR	1 cut(s)	1161
PshAI	GACNNNNGTC	1 cut(s)	1830
PsiI	TTATAA	1 cut(s)	492
Psp124BI	GAGCTC	1 cut(s)	234
Psp6I	CCWGG	1 cut(s)	212
PspGI	CCWGG	1 cut(s)	212
PstI	CTGCAG	1 cut(s)	100
PstNI	CAGNNNCTG	1 cut(s)	1642
PsuI	RGATCY	1 cut(s)	241
PvuII	CAGCTG	1 cut(s)	160
RsaI	GTAC	5 cut(s)	40, 806, 956, 1163, 1328
RsaNI	GTAC	5 cut(s)	39, 805, 955, 1162, 1327
RseI	CAYNNNNRTG	2 cut(s)	1733, 1778
SacI	GAGCTC	1 cut(s)	234
SaqAI	TTAA	7 cut(s)	207, 549, 924, 1344, 1398, 1631, 1871
SatI	GCNGC	6 cut(s)	833, 897, 1146, 1314, 1784, 1881
Sau3AI	GATC	6 cut(s)	241, 345, 571, 726, 1192, 1278
SchI	GAGTC	3 cut(s)	100, 512, 1704
ScrFI	CCNGG	2 cut(s)	214, 1405
SduI	GDGCHC	4 cut(s)	234, 668, 889, 1749
SfaNI	GCATC	7 cut(s)	856, 1096, 1273, 1513, 1705, 1770, 1782
SfcI	CTRYAG	2 cut(s)	96, 1647
Sfr274I	CTCGAG	1 cut(s)	507
SlaI	CTCGAG	1 cut(s)	507
SmiMI	CAYNNNNRTG	2 cut(s)	1733, 1778
SmlI	CTYRAG	2 cut(s)	507, 814
SmoI	CTYRAG	2 cut(s)	507, 814
SnaBI	TACGTA	1 cut(s)	1161
SphI	GCATGC	2 cut(s)	68, 1790
SseBI	AGGCCT	2 cut(s)	1350, 1455
SsiI	CCGC	3 cut(s)	28, 123, 300
SspMI	CTAG	3 cut(s)	315, 522, 1754
SstI	GAGCTC	1 cut(s)	234
StuI	AGGCCT	2 cut(s)	1350, 1455
StyD4I	CCNGG	2 cut(s)	212, 1403
StyI	CCWWGG	1 cut(s)	1578
TaaI	ACNGT	1 cut(s)	631
TaiI	ACGT	3 cut(s)	851, 1163, 1413
TatI	WGTACW	3 cut(s)	804, 954, 1326
TfiI	GAWTC	3 cut(s)	71, 473, 1559
Tru1I	TTAA	7 cut(s)	207, 549, 924, 1344, 1398, 1631, 1871
Tru9I	TTAA	7 cut(s)	207, 549, 924, 1344, 1398, 1631, 1871
TscAI	CASTG	5 cut(s)	111, 673, 1011, 1705, 1825
TseFI	GTSAC	5 cut(s)	516, 844, 1054, 1615, 1865
TseI	GCWGC	6 cut(s)	832, 896, 1145, 1313, 1783, 1880
Tsp45I	GTSAC	5 cut(s)	516, 844, 1054, 1615, 1865
TspDTI	ATGAA	2 cut(s)	615, 1082
TspGWI	ACGGA	2 cut(s)	640, 1524
TspRI	CASTG	5 cut(s)	111, 673, 1011, 1705, 1825
VneI	GTGCAC	2 cut(s)	664, 885
XagI	CCTNNNNNAGG	1 cut(s)	612
XapI	RAATTY	2 cut(s)	733, 1009
XbaI	TCTAGA	1 cut(s)	314
XceI	RCATGY	5 cut(s)	45, 68, 182, 664, 1790
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1096
XhoI	CTCGAG	1 cut(s)	507
XmiI	GTMKAC	2 cut(s)	641, 1650
XmnI	GAANNNNTTC	2 cut(s)	1516, 1889
XspI	CTAG	3 cut(s)	315, 522, 1754
Zsp2I	ATGCAT	3 cut(s)	871, 1111, 1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.