Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0017144

Physical Location & Seq

Genomic Coordinates Forward (+)

64002010 .. 64003929

Gene Structure 1920 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00271670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGTTGAAGCTCTCCCGTCCGAACAGGACACTCTTCGCCAGAGCATTCGGTTCATCGTCGTCTTCTTCTTCGCCGGCCGATTTCACCAGCACTATACTGAATTGCAGAAGCCCTACAGAAGCGTTTGAACTCTTCAATTCGGCGTCGAAACAAGTCGGCCCAAGGAAAGATCTTCAGCTTCATTCAGCGATTACCCATGTGTTGGTCGGAGCCAAACTATATATTAAAGCGAGGTTCTTGATAAAAGACTTGATATTGGATCTTCAGAAGTCCTGCAAATCCCGCCGTGCCTGTGACTTGGTCTACAATGCACTTGGCCGTTTGGAGAGGTCTAGACTCTCTGCTCGGAATGTTTTTGGAGTTTTGATCATTGCGTTATCTGAGATGGGTCTTGTTGAAGAAGCTTTGCGGGTGTATAGCAGCATTGGGTTGTTGCCTGCAACCCAGGCTTGTAATGCGCTCTTGGATGGGTTGGTGAAGACGGGTCAGCTTGATTCGATGTGGGAGCTTTATAATGATATGGACTCTCGCGGATTAAGACCTAGTTGTGTTACATATGGGGTGTTGATTGAGGGTTGTTGTAAGAAAGGCGATCTCGTGAATGCACGTAAGGTGTTCGATGAAATGCCTGAGAGAGGGATTGAAAGGACAGTTGTGGTGTATACGTCTCTTATTCGTGGCCTGTGCAATGAGGGGAGGATGGTGGAAGCTGAAAGCATTTTTCGGACGATGAGGAAAGATGGTGTGATACCAAATCTGTGGACTTACAACTGTCTGATGGATGGGTATTGCAAAGTGGCTAATGTTAAACAAGCCCTTGTGTTGCACTCGAACTTGGTAGGCGATGGCTTGCAGCCGAATGTTGTAACATTTGGTATTTTAGTTGATGCATTTTGCAAAGTAGGTGAACTGATGGCTGCGAGGAGCATTTTAGCGTGCATGGTTAAGTTTGGTGTTGCGCCTAATACGTTTGTGTACAATGCTTTGATTGACGGGCATTGTAAGGTAGAAGATGTTTCGGAAGCAATGGATTTGAAATTGGAGATGGAAAAGTATGGTGTTCTTCCGGATGTGTTCACTTACAGCATACTAATTAAGGGTCTATGTTCTGTTTGTAGAGTGGAAGAGGCGTATCAGTTGTTGCAAAACATGAAGAAGGAGGGAGTTGCTGCAAATGCTGTTACGTACAATTCACTAATCGATGGGTATTGTAAAGATCGCAATATGGAGAAGGCTTTGGAGGTGTGTTCTGAAATGACCAAAATTGGTATAGAACCAAATGTCATCACCTTCTCTACTATGATCGATGCTTACTGCAAGATGGGGAACATGGATGCTGCTATGGGTTTGTACTCGGAAATGGTAGTTAAAGGCCTTCTGCCTGATGTAGTAGCTTACACAGCTTTGATTGATGGCCATTTTAAAACCGGGAACGTGAAAGAGGCTCATCGCCTGCATAAAGAGATGTTGGAGGCAGGCCTCACGCCCAATGTTTTCACTCTTAGTTGCTTGGTAGATGGCCTTGGCAAAAATGGAAAGAGTTCTGATGCAATCAACCTCTTTTCAGAGAAAACTAAAGTCAGTTCCAATACAGATGAAACCAAGGAAATGGATGGTGACTTCTGTTCCCCAAATCATGTGACTTATACAGCTTTGATTCAGGGTCTGTCTGTAGACAACCGGATTTTTGAGGCAACCAAGTTCTTTTTGGATATGAGAGTCAGTGGTTTGAGACCAGATGCATTCACTTACATTGTCATGTTAAAGGACCACTTCCAAGCGAAGCATATGCTTGGCGTGATGATGCTGCATGCAGATATGATGAAGACGGGTATCATTCCCAGTATAGATATACGTCCGGTTTTATGTAGGGCTTATCAAGAAATTGGTGACCTAAAATCAGCAGCTAAGTTATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

639

Amino Acids

70.63

Weight (kDa)

7.42

Isoelectric Point (pI)

32.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	148 - 195	6.1e-15	PPR repeat family
PPR_long	PF17177	160 - 305	4e-12	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	177 - 209	1.5e-10	PPR repeat
PPR_2	PF13041	184 - 230	2e-13	PPR repeat family
PPR	PF01535	184 - 214	3.6e-08	PPR repeat
PPR_3	PF13812	204 - 263	1.5e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	212 - 245	3.5e-09	PPR repeat
PPR_2	PF13041	217 - 265	4e-16	PPR repeat family
PPR	PF01535	219 - 249	5.5e-08	PPR repeat
PPR_1	PF12854	248 - 275	1.6e-06	PPR repeat
PPR_2	PF13041	251 - 300	5.3e-13	PPR repeat family
PPR_3	PF13812	277 - 331	1.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	282 - 314	1.2e-07	PPR repeat
PPR_2	PF13041	286 - 335	3.3e-11	PPR repeat family
PPR_1	PF12854	318 - 350	4.8e-08	PPR repeat
PPR_2	PF13041	321 - 369	1.1e-18	PPR repeat family
PPR	PF01535	325 - 354	6.1e-06	PPR repeat
PPR_1	PF12854	353 - 385	2.1e-10	PPR repeat
PPR	PF01535	359 - 389	7.3e-06	PPR repeat
PPR_2	PF13041	369 - 405	1.6e-10	PPR repeat family
PPR_long	PF17177	374 - 484	9.3e-13	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	380 - 440	1.9e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	388 - 420	4.6e-11	PPR repeat
PPR_2	PF13041	392 - 440	5.2e-19	PPR repeat family
PPR	PF01535	394 - 424	1e-09	PPR repeat
PPR_1	PF12854	423 - 455	5.8e-12	PPR repeat
PPR	PF01535	429 - 459	1.1e-08	PPR repeat
PPR_2	PF13041	429 - 472	3.7e-13	PPR repeat family
PPR_3	PF13812	451 - 510	2.5e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	457 - 489	2e-08	PPR repeat
PPR_2	PF13041	461 - 510	4.4e-15	PPR repeat family
PPR	PF01535	464 - 494	8.6e-07	PPR repeat
PPR_1	PF12854	493 - 523	1.6e-06	PPR repeat
PPR_2	PF13041	544 - 589	1.5e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61400
fragaria_vesca	FvH4_5g14610
prunus_persica	Prupe.5G197300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0181881
rosa_laevigata	RLG00000005166
rosa_multiflora	Rmu_co8156836.1_g000001
rosa_roxburghii	Rroxscaffold_3G00271670
rosa_rugosa	Rorug06G0444800
rosa_samantha	Rh7AG047000 Rh7BG046600 Rh7CG047100 Rh7DG046200
rosa_wichuraiana	Rw7G003890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	513
AccB7I	CCANNNNNTGG	1 cut(s)	202
AccI	GTMKAC	3 cut(s)	303, 662, 1674
AccII	CGCG	1 cut(s)	531
AccIII	TCCGGA	1 cut(s)	1066
AciI	CCGC	3 cut(s)	283, 409, 531
AclWI	GGATC	1 cut(s)	267
AcoI	YGGCCR	3 cut(s)	75, 316, 1414
AcuI	CTGAAG	2 cut(s)	158, 248
AcyI	GRCGYC	1 cut(s)	143
AfaI	GTAC	3 cut(s)	977, 1187, 1352
AfiI	CCNNNNNNNGG	2 cut(s)	202, 635
AgsI	TTSAA	6 cut(s)	7, 128, 136, 398, 644, 1036
AjnI	CCWGG	1 cut(s)	444
Alw26I	GTCTC	2 cut(s)	672, 1726
AlwI	GGATC	1 cut(s)	267
AlwNI	CAGNNNCTG	1 cut(s)	1666
Aor13HI	TCCGGA	1 cut(s)	1066
AoxI	GGCC	8 cut(s)	75, 157, 316, 679, 1372, 1414, 1477, 1519
ApeKI	GCWGC	7 cut(s)	420, 853, 917, 1169, 1337, 1807, 1904
Asp700I	GAANNNNTTC	1 cut(s)	1540
AspLEI	GCGC	2 cut(s)	460, 961
AspS9I	GGNCC	2 cut(s)	158, 1768
AsuC2I	CCSGG	1 cut(s)	1429
AsuHPI	GGTGA	6 cut(s)	76, 487, 917, 1279, 1628, 1901
AvaII	GGWCC	1 cut(s)	1768
BalI	TGGCCA	1 cut(s)	1416
BauI	CACGAG	1 cut(s)	596
BbsI	GAAGAC	3 cut(s)	54, 485, 1832
BbvI	GCAGC	7 cut(s)	432, 865, 904, 1156, 1324, 1794, 1916
BceAI	ACGGC	2 cut(s)	270, 303
BcgI	CGANNNNNNTGC	2 cut(s)	1771, 1805
BciT130I	CCWGG	1 cut(s)	446
BclI	TGATCA	1 cut(s)	366
BcnI	CCSGG	1 cut(s)	1429
BcoDI	GTCTC	2 cut(s)	672, 1726
BfaI	CTAG	2 cut(s)	333, 543
BfmI	CTRYAG	2 cut(s)	114, 1671
BglII	AGATCT	1 cut(s)	169
BisI	GCNGC	7 cut(s)	421, 854, 918, 1170, 1338, 1808, 1905
BlsI	GCNGC	7 cut(s)	422, 855, 919, 1171, 1339, 1809, 1906
Bme1390I	CCNGG	2 cut(s)	446, 1429
Bme18I	GGWCC	1 cut(s)	1768
BmgT120I	GGNCC	2 cut(s)	158, 1768
BmiI	GGNNCC	1 cut(s)	211
BmrFI	CCNGG	2 cut(s)	446, 1429
BmrI	ACTGGG	1 cut(s)	1836
BmsI	GCATC	6 cut(s)	877, 1297, 1324, 1537, 1729, 1794
BmuI	ACTGGG	1 cut(s)	1836
BpiI	GAAGAC	3 cut(s)	54, 485, 1832
BpuMI	CCSGG	1 cut(s)	1429
Bsa29I	ATCGAT	2 cut(s)	1200, 1305
BsaAI	YACGTR	2 cut(s)	608, 1185
BsaHI	GRCGYC	1 cut(s)	143
BsaI	GGTCTC	1 cut(s)	1726
BsaJI	CCNNGG	4 cut(s)	161, 444, 1522, 1602
BsaWI	WCCGGW	3 cut(s)	1066, 1680, 1858
Bsc4I	CCNNNNNNNGG	2 cut(s)	202, 635
Bse118I	RCCGGY	1 cut(s)	73
Bse1I	ACTGG	1 cut(s)	1842
Bse3DI	GCAATG	3 cut(s)	369, 694, 1032
BseAI	TCCGGA	1 cut(s)	1066
BseBI	CCWGG	1 cut(s)	446
BseCI	ATCGAT	2 cut(s)	1200, 1305
BseDI	CCNNGG	4 cut(s)	161, 444, 1522, 1602
BseGI	GGATG	6 cut(s)	472, 705, 787, 1075, 1339, 1618
BseLI	CCNNNNNNNGG	2 cut(s)	202, 635
BseMI	GCAATG	3 cut(s)	369, 694, 1032
BseMII	CTCAG	2 cut(s)	372, 621
BseNI	ACTGG	1 cut(s)	1842
BseRI	GAGGAG	1 cut(s)	937
BseX3I	CGGCCG	1 cut(s)	75
BseXI	GCAGC	7 cut(s)	432, 865, 904, 1156, 1324, 1794, 1916
Bsh1236I	CGCG	1 cut(s)	531
Bsh1285I	CGRYCG	1 cut(s)	78
BshFI	GGCC	8 cut(s)	77, 159, 318, 681, 1374, 1416, 1479, 1521
BshVI	ATCGAT	2 cut(s)	1200, 1305
BsiEI	CGRYCG	1 cut(s)	78
BsiSI	CCGG	5 cut(s)	74, 1067, 1428, 1681, 1859
BslI	CCNNNNNNNGG	2 cut(s)	202, 635
BsmAI	GTCTC	2 cut(s)	672, 1726
BsmBI	CGTCTC	1 cut(s)	672
BsmI	GAATGC	3 cut(s)	44, 607, 1742
BsnI	GGCC	8 cut(s)	77, 159, 318, 681, 1374, 1416, 1479, 1521
Bso31I	GGTCTC	1 cut(s)	1726
Bsp13I	TCCGGA	1 cut(s)	1066
Bsp1407I	TGTACA	1 cut(s)	975
Bsp143I	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
BspACI	CCGC	3 cut(s)	283, 409, 531
BspANI	GGCC	8 cut(s)	77, 159, 318, 681, 1374, 1416, 1479, 1521
BspCNI	CTCAG	2 cut(s)	373, 622
BspDI	ATCGAT	2 cut(s)	1200, 1305
BspEI	TCCGGA	1 cut(s)	1066
BspFNI	CGCG	1 cut(s)	531
BspLI	GGNNCC	1 cut(s)	211
BspPI	GGATC	1 cut(s)	267
BspTNI	GGTCTC	1 cut(s)	1726
BsrDI	GCAATG	3 cut(s)	369, 694, 1032
BsrFI	RCCGGY	1 cut(s)	73
BsrGI	TGTACA	1 cut(s)	975
BsrI	ACTGG	1 cut(s)	1842
BssAI	RCCGGY	1 cut(s)	73
BssECI	CCNNGG	4 cut(s)	161, 444, 1522, 1602
BssMI	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
BssNAI	GTATAC	1 cut(s)	663
BssNI	GRCGYC	1 cut(s)	143
BssSI	CACGAG	1 cut(s)	596
BssT1I	CCWWGG	3 cut(s)	161, 1522, 1602
Bst1107I	GTATAC	1 cut(s)	663
Bst2BI	CACGAG	1 cut(s)	596
Bst2UI	CCWGG	1 cut(s)	446
Bst4CI	ACNGT	2 cut(s)	652, 773
Bst6I	CTCTTC	3 cut(s)	38, 137, 1119
BstACI	GRCGYC	1 cut(s)	143
BstAUI	TGTACA	1 cut(s)	975
BstBAI	YACGTR	2 cut(s)	608, 1185
BstC8I	GCNNGC	7 cut(s)	75, 438, 851, 937, 1454, 1477, 1812
BstDEI	CTNAG	4 cut(s)	381, 630, 1502, 1908
BstEII	GGTNACC	1 cut(s)	1889
BstENI	CCTNNNNNAGG	1 cut(s)	633
BstF5I	GGATG	6 cut(s)	472, 705, 787, 1075, 1339, 1618
BstFNI	CGCG	1 cut(s)	531
BstHHI	GCGC	2 cut(s)	460, 961
BstKTI	GATC	6 cut(s)	172, 262, 369, 595, 1219, 1305
BstMAI	GTCTC	2 cut(s)	672, 1726
BstMBI	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
BstMCI	CGRYCG	1 cut(s)	78
BstMWI	GCNNNNNNNGC	5 cut(s)	282, 446, 455, 1175, 1400
BstNI	CCWGG	1 cut(s)	446
BstNSI	RCATGY	1 cut(s)	1814
BstPI	GGTNACC	1 cut(s)	1889
BstSCI	CCNGG	2 cut(s)	444, 1427
BstSFI	CTRYAG	2 cut(s)	114, 1671
BstSNI	TACGTA	1 cut(s)	1185
BstUI	CGCG	1 cut(s)	531
BstV1I	GCAGC	7 cut(s)	432, 865, 904, 1156, 1324, 1794, 1916
BstV2I	GAAGAC	3 cut(s)	54, 485, 1832
BstX2I	RGATCY	2 cut(s)	169, 259
BstXI	CCANNNNNNTGG	2 cut(s)	759, 1609
BstYI	RGATCY	2 cut(s)	169, 259
BstZ17I	GTATAC	1 cut(s)	663
BstZI	CGGCCG	1 cut(s)	75
Bsu15I	ATCGAT	2 cut(s)	1200, 1305
BsuRI	GGCC	8 cut(s)	77, 159, 318, 681, 1374, 1416, 1479, 1521
BsuTUI	ATCGAT	2 cut(s)	1200, 1305
BtgZI	GCGATG	2 cut(s)	858, 1433
BtsCI	GGATG	6 cut(s)	472, 705, 787, 1075, 1339, 1618
BtsIMutI	CAGTG	1 cut(s)	1729
Cac8I	GCNNGC	7 cut(s)	75, 438, 851, 937, 1454, 1477, 1812
CaiI	CAGNNNCTG	1 cut(s)	1666
CfoI	GCGC	2 cut(s)	460, 961
Cfr10I	RCCGGY	1 cut(s)	73
Cfr13I	GGNCC	2 cut(s)	158, 1768
ClaI	ATCGAT	2 cut(s)	1200, 1305
CseI	GACGC	1 cut(s)	132
Csp6I	GTAC	3 cut(s)	976, 1186, 1351
CviAII	CATG	7 cut(s)	197, 940, 1150, 1330, 1637, 1759, 1811
CviQI	GTAC	3 cut(s)	976, 1186, 1351
DdeI	CTNAG	4 cut(s)	381, 630, 1502, 1908
DpnI	GATC	6 cut(s)	171, 261, 368, 594, 1218, 1304
DpnII	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
DraI	TTTAAA	1 cut(s)	1423
EaeI	YGGCCR	3 cut(s)	75, 316, 1414
EagI	CGGCCG	1 cut(s)	75
Eam1104I	CTCTTC	3 cut(s)	38, 137, 1119
EarI	CTCTTC	3 cut(s)	38, 137, 1119
EclXI	CGGCCG	1 cut(s)	75
Eco105I	TACGTA	1 cut(s)	1185
Eco130I	CCWWGG	3 cut(s)	161, 1522, 1602
Eco147I	AGGCCT	2 cut(s)	1374, 1479
Eco31I	GGTCTC	1 cut(s)	1726
Eco47I	GGWCC	1 cut(s)	1768
Eco52I	CGGCCG	1 cut(s)	75
Eco57I	CTGAAG	2 cut(s)	158, 248
Eco91I	GGTNACC	1 cut(s)	1889
EcoNI	CCTNNNNNAGG	1 cut(s)	633
EcoO65I	GGTNACC	1 cut(s)	1889
EcoRII	CCWGG	1 cut(s)	444
EcoT14I	CCWWGG	3 cut(s)	161, 1522, 1602
EcoT22I	ATGCAT	2 cut(s)	892, 1744
ErhI	CCWWGG	3 cut(s)	161, 1522, 1602
Esp3I	CGTCTC	1 cut(s)	672
FaeI	CATG	7 cut(s)	200, 943, 1153, 1333, 1640, 1762, 1814
FalI	AAGNNNNNCTT	2 cut(s)	1757, 1789
FatI	CATG	7 cut(s)	196, 939, 1149, 1329, 1636, 1758, 1810
FauI	CCCGC	2 cut(s)	290, 402
FauNDI	CATATG	2 cut(s)	556, 1788
FbaI	TGATCA	1 cut(s)	366
FblI	GTMKAC	3 cut(s)	303, 662, 1674
Fnu4HI	GCNGC	7 cut(s)	421, 854, 918, 1170, 1338, 1808, 1905
FokI	GGATG	6 cut(s)	479, 712, 794, 1082, 1346, 1625
Fsp4HI	GCNGC	7 cut(s)	421, 854, 918, 1170, 1338, 1808, 1905
FspBI	CTAG	2 cut(s)	333, 543
GlaI	GCGC	2 cut(s)	459, 960
GluI	GCNGC	7 cut(s)	421, 854, 918, 1170, 1338, 1808, 1905
HaeIII	GGCC	8 cut(s)	77, 159, 318, 681, 1374, 1416, 1479, 1521
HapII	CCGG	5 cut(s)	74, 1067, 1428, 1681, 1859
HgaI	GACGC	1 cut(s)	132
HhaI	GCGC	2 cut(s)	460, 961
Hin1I	GRCGYC	1 cut(s)	143
Hin1II	CATG	7 cut(s)	200, 943, 1153, 1333, 1640, 1762, 1814
Hin6I	GCGC	2 cut(s)	458, 959
HinP1I	GCGC	2 cut(s)	458, 959
HindIII	AAGCTT	1 cut(s)	402
HinfI	GANTC	5 cut(s)	336, 494, 524, 1657, 1719
HpaII	CCGG	5 cut(s)	74, 1067, 1428, 1681, 1859
HphI	GGTGA	6 cut(s)	76, 487, 917, 1279, 1628, 1901
Hpy166II	GTNNAC	7 cut(s)	304, 663, 762, 908, 976, 1077, 1675
Hpy188III	TCNNGA	5 cut(s)	238, 333, 598, 1067, 1880
Hpy8I	GTNNAC	7 cut(s)	304, 663, 762, 908, 976, 1077, 1675
Hpy99I	CGWCG	2 cut(s)	61, 148
HpyAV	CCTTC	4 cut(s)	1150, 1225, 1300, 1385
HpyCH4III	ACNGT	2 cut(s)	652, 773
HpyCH4IV	ACGT	6 cut(s)	607, 665, 968, 1184, 1434, 1855
HpyF10VI	GCNNNNNNNGC	5 cut(s)	282, 446, 455, 1175, 1400
HpyF3I	CTNAG	4 cut(s)	381, 630, 1502, 1908
HpySE526I	ACGT	6 cut(s)	607, 665, 968, 1184, 1434, 1855
Hsp92I	GRCGYC	1 cut(s)	143
Hsp92II	CATG	7 cut(s)	200, 943, 1153, 1333, 1640, 1762, 1814
HspAI	GCGC	2 cut(s)	458, 959
Kpn2I	TCCGGA	1 cut(s)	1066
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	366
Kzo9I	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
LmnI	GCTCC	3 cut(s)	209, 505, 924
Lsp1109I	GCAGC	7 cut(s)	432, 865, 904, 1156, 1324, 1794, 1916
LweI	GCATC	6 cut(s)	877, 1297, 1324, 1537, 1729, 1794
MaeI	CTAG	2 cut(s)	333, 543
MaeII	ACGT	6 cut(s)	607, 665, 968, 1184, 1434, 1855
MaeIII	GTNAC	7 cut(s)	293, 550, 865, 1180, 1616, 1639, 1889
MalI	GATC	6 cut(s)	171, 261, 368, 594, 1218, 1304
MboI	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
MflI	RGATCY	2 cut(s)	169, 259
MlsI	TGGCCA	1 cut(s)	1416
MluCI	AATT	7 cut(s)	100, 136, 1037, 1092, 1189, 1263, 1884
MluNI	TGGCCA	1 cut(s)	1416
MlyI	GAGTC	3 cut(s)	330, 518, 1728
MmeI	TCCRAC	2 cut(s)	187, 1449
Mox20I	TGGCCA	1 cut(s)	1416
Mph1103I	ATGCAT	2 cut(s)	892, 1744
MroI	TCCGGA	1 cut(s)	1066
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	1 cut(s)	1540
MscI	TGGCCA	1 cut(s)	1416
MseI	TTAA	8 cut(s)	225, 536, 807, 945, 1095, 1368, 1422, 1763
MslI	CAYNNNNRTG	1 cut(s)	1757
Msp20I	TGGCCA	1 cut(s)	1416
MspI	CCGG	5 cut(s)	74, 1067, 1428, 1681, 1859
MspR9I	CCNGG	2 cut(s)	446, 1429
Mva1269I	GAATGC	3 cut(s)	44, 607, 1742
MvaI	CCWGG	1 cut(s)	446
MvnI	CGCG	1 cut(s)	531
MwoI	GCNNNNNNNGC	5 cut(s)	282, 446, 455, 1175, 1400
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	1 cut(s)	1429
NdeI	CATATG	2 cut(s)	556, 1788
NdeII	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
NgoMIV	GCCGGC	1 cut(s)	73
NlaIII	CATG	7 cut(s)	200, 943, 1153, 1333, 1640, 1762, 1814
NlaIV	GGNNCC	1 cut(s)	211
NmuCI	GTSAC	4 cut(s)	293, 1616, 1639, 1889
NsiI	ATGCAT	2 cut(s)	892, 1744
NspI	RCATGY	1 cut(s)	1814
PaeI	GCATGC	1 cut(s)	1814
PceI	AGGCCT	2 cut(s)	1374, 1479
PctI	GAATGC	3 cut(s)	44, 607, 1742
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	1 cut(s)	1540
PfeI	GAWTC	2 cut(s)	494, 1657
PflFI	GACNNNGTC	1 cut(s)	299
PflMI	CCANNNNNTGG	1 cut(s)	202
PkrI	GCNGC	7 cut(s)	422, 855, 919, 1171, 1339, 1809, 1906
PleI	GAGTC	3 cut(s)	330, 518, 1727
PpsI	GAGTC	3 cut(s)	330, 518, 1727
Ppu21I	YACGTR	2 cut(s)	608, 1185
PsiI	TTATAA	1 cut(s)	513
Psp6I	CCWGG	1 cut(s)	444
PspEI	GGTNACC	1 cut(s)	1889
PspGI	CCWGG	1 cut(s)	444
PspN4I	GGNNCC	1 cut(s)	211
PspPI	GGNCC	2 cut(s)	158, 1768
PstNI	CAGNNNCTG	1 cut(s)	1666
PsuI	RGATCY	2 cut(s)	169, 259
PsyI	GACNNNGTC	1 cut(s)	299
RsaI	GTAC	3 cut(s)	977, 1187, 1352
RsaNI	GTAC	3 cut(s)	976, 1186, 1351
RseI	CAYNNNNRTG	1 cut(s)	1757
SaqAI	TTAA	8 cut(s)	225, 536, 807, 945, 1095, 1368, 1422, 1763
SatI	GCNGC	7 cut(s)	421, 854, 918, 1170, 1338, 1808, 1905
Sau3AI	GATC	6 cut(s)	169, 259, 366, 592, 1216, 1302
Sau96I	GGNCC	2 cut(s)	158, 1768
SchI	GAGTC	3 cut(s)	330, 518, 1728
ScrFI	CCNGG	2 cut(s)	446, 1429
SfaNI	GCATC	6 cut(s)	877, 1297, 1324, 1537, 1729, 1794
SfcI	CTRYAG	2 cut(s)	114, 1671
SinI	GGWCC	1 cut(s)	1768
SmiMI	CAYNNNNRTG	1 cut(s)	1757
SnaBI	TACGTA	1 cut(s)	1185
SphI	GCATGC	1 cut(s)	1814
Sse9I	AATT	7 cut(s)	100, 136, 1037, 1092, 1189, 1263, 1884
SseBI	AGGCCT	2 cut(s)	1374, 1479
SsiI	CCGC	3 cut(s)	283, 409, 531
SspMI	CTAG	2 cut(s)	333, 543
StuI	AGGCCT	2 cut(s)	1374, 1479
StyD4I	CCNGG	2 cut(s)	444, 1427
StyI	CCWWGG	3 cut(s)	161, 1522, 1602
TaaI	ACNGT	2 cut(s)	652, 773
TaiI	ACGT	6 cut(s)	610, 668, 971, 1187, 1437, 1858
TaqI	TCGA	6 cut(s)	146, 497, 618, 830, 1200, 1305
TasI	AATT	7 cut(s)	100, 136, 1037, 1092, 1189, 1263, 1884
TatI	WGTACW	2 cut(s)	975, 1350
TfiI	GAWTC	2 cut(s)	494, 1657
Tru1I	TTAA	8 cut(s)	225, 536, 807, 945, 1095, 1368, 1422, 1763
Tru9I	TTAA	8 cut(s)	225, 536, 807, 945, 1095, 1368, 1422, 1763
TscAI	CASTG	1 cut(s)	1729
TseFI	GTSAC	4 cut(s)	293, 1616, 1639, 1889
TseI	GCWGC	7 cut(s)	420, 853, 917, 1169, 1337, 1807, 1904
Tsp45I	GTSAC	4 cut(s)	293, 1616, 1639, 1889
TspDTI	ATGAA	6 cut(s)	42, 170, 636, 1166, 1611, 1838
TspRI	CASTG	1 cut(s)	1729
Tth111I	GACNNNGTC	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	202
VpaK11BI	GGWCC	1 cut(s)	1768
XagI	CCTNNNNNAGG	1 cut(s)	633
XbaI	TCTAGA	1 cut(s)	332
XceI	RCATGY	1 cut(s)	1814
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1705
XmiI	GTMKAC	3 cut(s)	303, 662, 1674
XmnI	GAANNNNTTC	1 cut(s)	1540
XspI	CTAG	2 cut(s)	333, 543
Zsp2I	ATGCAT	2 cut(s)	892, 1744

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.