FvH4_5g15060

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016340
Physical Location & Seq
Reverse (-)
8521985 .. 8526447
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15060.t2

Sequence Viewer

Length: 1491 bp
ATGCCTCACAGAAATGTTGTTACTGTTAATGGTATGCTTTCTGGGTTTGTGAAGAATGGTATGTTAGATGAGGGGATTGGTTTGTTCAAGAAAGTCTTGGGTTGCTCTTTTGGTTTGGAATGTAGGCCAAATTATGTAACTTTGGTTGTTTTGGTTTCGGGGTGTGTGGAACTGGATGGGTTTATTGTGGGGAGGGTGCTTCATTCACACTGTTGTAAGGCAGGTTTGGATTTGGTGAATGAGGTTTGTAATGCACTGATCGATTTGTATTCGAAGTTTGGGCATTTGGGTGAAGCAGTGAGGATGTTTAATGAGATGCCTGAGAGGGATTTGGTTTCGTGGAATTCCATGATTGCAGGGTATGCCGGGGTTGGTGAGTGTAGAAAAGCTTTGTACTTGTTTAGGGAAATGAGAGCAGGCAACGTTGGATTTGATAGAGTGTCGTTGAGTAGTTTGATTTCGGCTGCTGCTAATGGTCGAGATCTTGGTATGGGGAAGGTGGTTCATGGGTATATGACAGTTAGTGGAGCGGAGATCACTGTGGCTATTGGAACTGCTCTGATTGATATGTATTCCAAATGTGGATCAACAAAGTGTGCTAGAAAAATCTTGGACAATCTTCAAGATGAAAGCATTGTGTTGTGGAACTCTATGATTCATGGATATGTCGAATGTGGTCATAATCAAGAGGCATTAGAGCTATTCAATCATATTCAATCAAGAAAGCTAAGGCCAGATGAAGTAACAATGGTAAGATTAATCCTAGCTTGCCGCAACTCAGGGGATTTGTCTCATGGTATTGGCATTCATTCTTATATAGAGAATGACATACATCTTCAAAGCAGCATTGTACTGCACAATGCTCTTATTGATATGTACTCGAAGTGCGGGAACATGAGCCTTGCTAAAGTTGTGTTTAATAAGATGCCTAGCAAAGATGTTATCTCATGGACTTCAATTATAGTGGGTCACGCGATCAATGGTGAAGGAAAGGAGGCTATTCTTGCTTTCAGAAAAATGCGTGCTGAGAAAGTGGAGCCGAACTCTGTTACCTTCATAGGTGTTCTATCAGCATGTGACCATGCTGGTTTGGTTGATGAAGGTCTGAACTTGTATGATGATATGTGCAGGTTGTATCACATCAAGCCCCGGATTGAGCACTGCGGATGCATCATTGATATGCTTGCGCGAGCAGGAAGACTAGAGGAGGCACACAAGTTTGTGAAGAGTATGCCTATAGAACTAAATGCAGTTGTATATAGGATGTTGGTTAATGCGTGTCGAGTTCATGGTGATTTTGGTAGAGGATTGTGTTTGGTTAGTAGATTCGCAGATCTGAACACATTGCATGGTGCTGAAGATCATGTTGCTTCATCCAATATTCTAGCCGATGCAGGAAGGTGGGATGATGTTCTTCACGAAAGGAGCTTGATGGCCACCAGGACATGTACAAAAGTTGCTGGTAAAAGCTCTATTACAGACTTCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

497

Amino Acids

54.53

Weight (kDa)

6.86

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 82 - 110 6.9e-06 PPR repeat
PPR_2 PF13041 110 - 157 8.5e-08 PPR repeat family
PPR PF01535 112 - 139 1.9e-06 PPR repeat
PPR_2 PF13041 211 - 257 2.7e-10 PPR repeat family
PPR PF01535 213 - 241 9.2e-06 PPR repeat
PPR_2 PF13041 312 - 359 1.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016340)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15060 FvH4_5g15060
malus_domestica MD02G1300800.v1.1 MD06G1163000.v1.1 MD12G1160800.v1.1 MD14G1199000.v1.1
prunus_persica Prupe.5G192100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0181141
rosa_laevigata RLG00000005210
rosa_multiflora Rmu_sc0001147.1_g000018
rosa_roxburghii Rroxscaffold_3G00272240
rosa_rugosa Rorug06G0438700
rosa_samantha Rh7BG040100 Rh7CG042100
rosa_wichuraiana Rw7G003360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 212, 1119
AccBSI CCGCTC 1 cut(s) 530
AccII CGCG 2 cut(s) 974, 1189
AciI CCGC 4 cut(s) 530, 772, 888, 1164
AclI AACGTT 1 cut(s) 423
AclWI GGATC 1 cut(s) 592
AcoI YGGCCR 1 cut(s) 1434
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 1377
AfaI GTAC 4 cut(s) 395, 852, 878, 1450
AflIII ACRYGT 1 cut(s) 1445
AgsI TTSAA 6 cut(s) 88, 623, 706, 716, 839, 957
AjnI CCWGG 1 cut(s) 1439
AluBI AGCT 6 cut(s) 389, 700, 727, 767, 1428, 1470
AluI AGCT 6 cut(s) 389, 700, 727, 767, 1428, 1470
Alw21I GWGCWC 1 cut(s) 1161
Alw26I GTCTC 1 cut(s) 795
AlwI GGATC 1 cut(s) 592
AoxI GGCC 3 cut(s) 125, 731, 1434
ApeKI GCWGC 3 cut(s) 464, 467, 843
ApoI RAATTY 1 cut(s) 343
AseI ATTAAT 1 cut(s) 758
AspLEI GCGC 1 cut(s) 1189
AsuC2I CCSGG 2 cut(s) 367, 1150
AsuHPI GGTGA 5 cut(s) 247, 302, 386, 995, 1304
AsuII TTCGAA 1 cut(s) 272
BalI TGGCCA 1 cut(s) 1436
BbsI GAAGAC 1 cut(s) 1204
Bbv12I GWGCWC 1 cut(s) 1161
BbvI GCAGC 3 cut(s) 451, 454, 855
BccI CCATC 2 cut(s) 170, 1426
BciT130I CCWGG 1 cut(s) 1441
BcnI CCSGG 2 cut(s) 367, 1150
BcoDI GTCTC 1 cut(s) 795
BfaI CTAG 5 cut(s) 600, 764, 930, 1202, 1385
BfmI CTRYAG 1 cut(s) 1236
BfuAI ACCTGC 2 cut(s) 212, 1119
BglII AGATCT 2 cut(s) 481, 1333
BisI GCNGC 4 cut(s) 465, 468, 772, 844
BlsI GCNGC 4 cut(s) 466, 469, 773, 845
Bme1390I CCNGG 3 cut(s) 367, 1150, 1441
BmiI GGNNCC 1 cut(s) 1038
BmrFI CCNGG 3 cut(s) 367, 1150, 1441
BmsI GCATC 5 cut(s) 306, 915, 1157, 1179, 1381
BpiI GAAGAC 1 cut(s) 1204
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
Bpu10I CCTNAGC 1 cut(s) 728
Bpu14I TTCGAA 1 cut(s) 272
BpuMI CCSGG 2 cut(s) 367, 1150
Bsa29I ATCGAT 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 366, 1148
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 1343
BseBI CCWGG 1 cut(s) 1441
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 366, 1148
BseGI GGATG 6 cut(s) 181, 309, 1172, 1269, 1373, 1411
BseMI GCAATG 1 cut(s) 1343
BseMII CTCAG 3 cut(s) 312, 792, 1017
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 1220
BseXI GCAGC 3 cut(s) 451, 454, 855
BsgI GTGCAG 2 cut(s) 839, 1147
Bsh1236I CGCG 2 cut(s) 974, 1189
BshFI GGCC 3 cut(s) 127, 733, 1436
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 1161
BsiSI CCGG 2 cut(s) 366, 1150
BsmAI GTCTC 1 cut(s) 795
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 3 cut(s) 127, 733, 1436
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 1161
Bsp1407I TGTACA 1 cut(s) 1448
Bsp143I GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
BspACI CCGC 4 cut(s) 530, 772, 888, 1164
BspANI GGCC 3 cut(s) 127, 733, 1436
BspCNI CTCAG 3 cut(s) 313, 791, 1018
BspDI ATCGAT 1 cut(s) 261
BspFNI CGCG 2 cut(s) 974, 1189
BspLI GGNNCC 1 cut(s) 1038
BspMI ACCTGC 2 cut(s) 212, 1119
BspPI GGATC 1 cut(s) 592
BspT104I TTCGAA 1 cut(s) 272
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 1343
BsrGI TGTACA 1 cut(s) 1448
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 366, 1148
BssMI GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
Bst2UI CCWGG 1 cut(s) 1441
Bst4CI ACNGT 4 cut(s) 25, 212, 520, 541
Bst6I CTCTTC 1 cut(s) 1220
BstAPI GCANNNNNTGC 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 1448
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 5 cut(s) 418, 769, 1023, 1185, 1191
BstDEI CTNAG 4 cut(s) 321, 728, 778, 1026
BstF5I GGATG 6 cut(s) 181, 309, 1172, 1269, 1373, 1411
BstFNI CGCG 2 cut(s) 974, 1189
BstHHI GCGC 1 cut(s) 1189
BstKTI GATC 7 cut(s) 261, 484, 537, 587, 978, 1336, 1363
BstMAI GTCTC 1 cut(s) 795
BstMBI GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
BstMWI GCNNNNNNNGC 2 cut(s) 362, 1004
BstNI CCWGG 1 cut(s) 1441
BstNSI RCATGY 2 cut(s) 1077, 1449
BstSCI CCNGG 3 cut(s) 365, 1148, 1439
BstSFI CTRYAG 1 cut(s) 1236
BstUI CGCG 2 cut(s) 974, 1189
BstV1I GCAGC 3 cut(s) 451, 454, 855
BstV2I GAAGAC 1 cut(s) 1204
BstX2I RGATCY 2 cut(s) 481, 1333
BstYI RGATCY 2 cut(s) 481, 1333
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 3 cut(s) 127, 733, 1436
BsuTUI ATCGAT 1 cut(s) 261
BtsCI GGATG 6 cut(s) 181, 309, 1172, 1269, 1373, 1411
BtsI GCAGTG 2 cut(s) 303, 1159
BtsIMutI CAGTG 5 cut(s) 208, 254, 303, 537, 1159
BveI ACCTGC 2 cut(s) 212, 1119
Cac8I GCNNGC 5 cut(s) 418, 769, 1023, 1185, 1191
CfoI GCGC 1 cut(s) 1189
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 4 cut(s) 394, 851, 877, 1449
CspCI CAANNNNNGTGG 2 cut(s) 945, 980
CviQI GTAC 4 cut(s) 394, 851, 877, 1449
DdeI CTNAG 4 cut(s) 321, 728, 778, 1026
DpnI GATC 7 cut(s) 260, 483, 536, 586, 977, 1335, 1362
DpnII GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
EaeI YGGCCR 1 cut(s) 1434
Eam1104I CTCTTC 1 cut(s) 1220
EarI CTCTTC 1 cut(s) 1220
Eco57I CTGAAG 1 cut(s) 1377
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 1439
EcoT22I ATGCAT 1 cut(s) 1172
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FauI CCCGC 1 cut(s) 881
Fnu4HI GCNGC 4 cut(s) 465, 468, 772, 844
FokI GGATG 6 cut(s) 188, 316, 1179, 1276, 1360, 1418
Fsp4HI GCNGC 4 cut(s) 465, 468, 772, 844
FspBI CTAG 5 cut(s) 600, 764, 930, 1202, 1385
GlaI GCGC 1 cut(s) 1188
GluI GCNGC 4 cut(s) 465, 468, 772, 844
HaeIII GGCC 3 cut(s) 127, 733, 1436
HapII CCGG 2 cut(s) 366, 1150
HhaI GCGC 1 cut(s) 1189
Hin6I GCGC 1 cut(s) 1187
HinP1I GCGC 1 cut(s) 1187
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 2 cut(s) 655, 1326
HpaII CCGG 2 cut(s) 366, 1150
HphI GGTGA 5 cut(s) 247, 302, 386, 995, 1304
Hpy188I TCNGA 5 cut(s) 561, 1013, 1107, 1338, 1487
Hpy188III TCNNGA 6 cut(s) 88, 479, 623, 686, 720, 1418
HpyAV CCTTC 5 cut(s) 490, 980, 1063, 1094, 1392
HpyCH4III ACNGT 4 cut(s) 25, 212, 520, 541
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 8 cut(s) 254, 356, 856, 1128, 1170, 1250, 1348, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 1004
HpyF3I CTNAG 4 cut(s) 321, 728, 778, 1026
HpySE526I ACGT 1 cut(s) 423
HspAI GCGC 1 cut(s) 1187
Kzo9I GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
LmnI GCTCC 3 cut(s) 527, 1036, 1425
Lsp1109I GCAGC 3 cut(s) 451, 454, 855
LweI GCATC 5 cut(s) 306, 915, 1157, 1179, 1381
MaeI CTAG 5 cut(s) 600, 764, 930, 1202, 1385
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 6 cut(s) 19, 136, 742, 968, 1048, 1076
MalI GATC 7 cut(s) 260, 483, 536, 586, 977, 1335, 1362
MbiI CCGCTC 1 cut(s) 530
MboI GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
MboII GAAGA 7 cut(s) 64, 611, 827, 1209, 1237, 1370, 1406
MflI RGATCY 2 cut(s) 481, 1333
MhlI GDGCHC 1 cut(s) 1161
MlsI TGGCCA 1 cut(s) 1436
MluCI AATT 3 cut(s) 130, 343, 957
MluNI TGGCCA 1 cut(s) 1436
MmeI TCCRAC 1 cut(s) 406
Mox20I TGGCCA 1 cut(s) 1436
Mph1103I ATGCAT 1 cut(s) 1172
MscI TGGCCA 1 cut(s) 1436
MseI TTAA 5 cut(s) 27, 309, 758, 918, 1272
MslI CAYNNNNRTG 4 cut(s) 12, 288, 663, 1178
Msp20I TGGCCA 1 cut(s) 1436
MspI CCGG 2 cut(s) 366, 1150
MspR9I CCNGG 3 cut(s) 367, 1150, 1441
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 1 cut(s) 1441
MvnI CGCG 2 cut(s) 974, 1189
MwoI GCNNNNNNNGC 2 cut(s) 362, 1004
NciI CCSGG 2 cut(s) 367, 1150
NdeII GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
NlaIV GGNNCC 1 cut(s) 1038
NmuCI GTSAC 2 cut(s) 968, 1076
NsiI ATGCAT 1 cut(s) 1172
NspI RCATGY 2 cut(s) 1077, 1449
NspV TTCGAA 1 cut(s) 272
PciI ACATGT 1 cut(s) 1445
PctI GAATGC 1 cut(s) 804
PfeI GAWTC 2 cut(s) 655, 1326
PkrI GCNGC 4 cut(s) 466, 469, 773, 845
PscI ACATGT 1 cut(s) 1445
PshBI ATTAAT 1 cut(s) 758
Psp1406I AACGTT 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 1439
PspGI CCWGG 1 cut(s) 1439
PspN4I GGNNCC 1 cut(s) 1038
PsuI RGATCY 2 cut(s) 481, 1333
RsaI GTAC 4 cut(s) 395, 852, 878, 1450
RsaNI GTAC 4 cut(s) 394, 851, 877, 1449
RseI CAYNNNNRTG 4 cut(s) 12, 288, 663, 1178
SaqAI TTAA 5 cut(s) 27, 309, 758, 918, 1272
SatI GCNGC 4 cut(s) 465, 468, 772, 844
Sau3AI GATC 7 cut(s) 258, 481, 534, 584, 975, 1333, 1360
ScrFI CCNGG 3 cut(s) 367, 1150, 1441
SduI GDGCHC 1 cut(s) 1161
SfaNI GCATC 5 cut(s) 306, 915, 1157, 1179, 1381
SfcI CTRYAG 1 cut(s) 1236
SfuI TTCGAA 1 cut(s) 272
SmiMI CAYNNNNRTG 4 cut(s) 12, 288, 663, 1178
Sse9I AATT 3 cut(s) 130, 343, 957
SsiI CCGC 4 cut(s) 530, 772, 888, 1164
SspI AATATT 1 cut(s) 1381
SspMI CTAG 5 cut(s) 600, 764, 930, 1202, 1385
StyD4I CCNGG 3 cut(s) 365, 1148, 1439
TaaI ACNGT 4 cut(s) 25, 212, 520, 541
TaiI ACGT 1 cut(s) 426
TaqI TCGA 6 cut(s) 261, 272, 478, 669, 881, 1282
TasI AATT 3 cut(s) 130, 343, 957
TatI WGTACW 4 cut(s) 393, 850, 876, 1448
TauI GCSGC 1 cut(s) 774
TfiI GAWTC 2 cut(s) 655, 1326
Tru1I TTAA 5 cut(s) 27, 309, 758, 918, 1272
Tru9I TTAA 5 cut(s) 27, 309, 758, 918, 1272
TscAI CASTG 5 cut(s) 215, 261, 303, 544, 1166
TseFI GTSAC 2 cut(s) 968, 1076
TseI GCWGC 3 cut(s) 464, 467, 843
Tsp45I GTSAC 2 cut(s) 968, 1076
TspRI CASTG 5 cut(s) 215, 261, 303, 544, 1166
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 1 cut(s) 343
XceI RCATGY 2 cut(s) 1077, 1449
XspI CTAG 5 cut(s) 600, 764, 930, 1202, 1385
Zsp2I ATGCAT 1 cut(s) 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.