MD14G1199000.v1.1

PPR repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016340
Physical Location & Seq
Forward (+)
28895663 .. 28897687
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1199000.v1.1.491

Sequence Viewer

Length: 1959 bp
ATGAGCGCTTCATTCCCCCGCCAAAGACAGCGACTTTTCCATCTTCTCCACCAAAGCCCCGCCGTCTTCTCCTCCCAGAAGCTCCTTCAAATCCAGGCCCAGCTCACCACCACCGGCCTCCTCCACTCAGACCCCTCATGCCTTCTCTCACTCCTCGACCTCTCTCTCTCAACCCCTTCAACTCTCCACCACGCCACCGCCTTATTCACCCACGCACAAACACCCAATGCCGCCGCATGGCCCGTCATGGTCCGCCGGCTCAGCTTAGATGCCGACCCTTCTCGGGTTTTCGCTTTATTCAAAGAAATGCAGAGGCTAAAACGTAATGACCCAGTTTGTGATCCTTATGTGTTTGCTTCTTTGATCAAGGGTTGTAGCAAATTATCCGCTTTTCGGGAAGGGAAGTCTGTGCATTGCCGAGCTGTTAGGATCGGTTTGGATTGTAATGTGAATTTGTTGAACAGTTTAGTTAGTTTTTATTCGCGTTCGAAGAGTTTGGTGGGATGTGCTTGCATTGTGTTTGATAGAATTCCTCAGAAGACTGTTGTGACAGTAAATTGTATGCTTTCTGGTTTTGTGAGGAACAGCTTGTTTGGTGCGGCGTTGGATTTGTTTAACCGAGTTTTGAGTTGCGATTTTGGTTTGGGATTGAAGCCGAATTATGTTACTTTGGTTGTTTTGACTTCAGGGTGTGTGGATTTTGATGAGTTTAATGCGGGAAAGGCACTTCATTCATACTGTTATAAGGCGGATGAAGCTGGAAGGATGTTCGATGAGATGCCTGAGAGGGATTTGGTTTCATGGAATACCATGATTGCAGGATATGCTGGAGTTGGTGATTGTAGAAGAGCTTTCTTTTTGTTTAGAGAAATGAACGCTGGGGATGTTGGATTCGATAGAGTATCGCTGATAAGTTTGATTTTGGCTGCTTGTAATAGTAGAGATCTTGGTATGGGGAAGATGATTCACGGGTATATGACAGCTAATGGAACAAAAATAACTGTAGCTATTCGGACGGCACTGATTAACTTGTACTCCAATTGTGGACTAATAGCGTATGCTAAAAAAGTATTGGATGAAGCACCGGATGACAACATTGCATTGTGGAACTCTATGATCCATGGATATGTCGAATGCTGTCATAATCAAGAGGCATTGGGGCTCTTCAGTCAGATGCAGTCTAAAAAGCTAGAGCCAGATGAAACAACAATGGTAGGGTTAATCTTAGCTTGCCGAAACTCAGGGGATTTGTCTAATGGATTCGACATTCATTCTTACATAGAGAGTAGTAATCGTCATCAAGGGAGTATTGTTCTGCACAATGCTCTTATAGACATGTATGCAAAATGCGGGAGCATGACCCAAGCTAAAGTTGTGTTTGATAAGATGCCACGGAAAGACGTTGTTTCGTGGACTTCAATTATAGTTGGTCATGCCATCAACAGTGAAGGAAAGGAAGCGCTTCTTGCTTTCCAGAGAATGTGTGCTGAGAAAGTTGAACCAAGCTCAGTTACATTCATCAGTGTTCTATCAGCGTGCGATCATGCTGGTTTGGTTGATGAAGGTCTGAACTTGTATAACGCAATGTGCAAGTCTTACCATATCAAGCCCACAATTGAGCACTGCGGATGCATGATTGATATGTACGCACGAGCAGGAAGGCTAGAGGAGGCCTACAAGTTTGTGAAGAGTATGCCTGTAGAGCCAAATGCAGTTGTTTGGAGGATGTTGATTAATGCTTGTAGAGTTCATGGCGATTTTGATAGGGGATTATGTTTGGTTAGTGGATTCACAGAACTAAAAACACTGCATCGTGGTGCCGAAGATCATGTAACTTCTTCCAACATTCTTGCTGATGCAGGAAGGTGGGACGATGTTCTAAATGAAAGAAGCTTGATGGCGACGAGGAAAGTGACGAAAGTCTCAGGTAAAAGCTCCATTTCAGATTTAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

653

Amino Acids

71.98

Weight (kDa)

7.93

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 365 - 411 9.6e-10 PPR repeat family
PPR_2 PF13041 466 - 513 4.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016340)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15060 FvH4_5g15060
malus_domestica MD02G1300800.v1.1 MD06G1163000.v1.1 MD12G1160800.v1.1 MD14G1199000.v1.1
prunus_persica Prupe.5G192100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0181141
rosa_laevigata RLG00000005210
rosa_multiflora Rmu_sc0001147.1_g000018
rosa_roxburghii Rroxscaffold_3G00272240
rosa_rugosa Rorug06G0438700
rosa_samantha Rh7BG040100 Rh7CG042100
rosa_wichuraiana Rw7G003360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 1817
AccII CGCG 1 cut(s) 484
AclWI GGATC 3 cut(s) 335, 437, 1111
AcsI RAATTY 2 cut(s) 451, 528
AcuI CTGAAG 2 cut(s) 669, 1150
AfaI GTAC 2 cut(s) 1034, 1646
AfeI AGCGCT 2 cut(s) 7, 1461
AfiI CCNNNNNNNGG 3 cut(s) 237, 283, 393
AflIII ACRYGT 1 cut(s) 1335
AgsI TTSAA 7 cut(s) 89, 180, 301, 460, 652, 1419, 1499
AjnI CCWGG 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 1623
Alw26I GTCTC 1 cut(s) 1927
AlwI GGATC 3 cut(s) 335, 437, 1111
Ama87I CYCGRG 1 cut(s) 282
Aor51HI AGCGCT 2 cut(s) 7, 1461
AoxI GGCC 4 cut(s) 96, 115, 239, 1671
ApeKI GCWGC 1 cut(s) 926
ApoI RAATTY 2 cut(s) 451, 528
AseI ATTAAT 1 cut(s) 1734
Asp700I GAANNNNTTC 1 cut(s) 1461
AspLEI GCGC 2 cut(s) 8, 1462
AspS9I GGNCC 3 cut(s) 97, 240, 250
AsuHPI GGTGA 3 cut(s) 97, 199, 848
AsuII TTCGAA 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 282
AvaII GGWCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 1817
BanII GRGCYC 1 cut(s) 1164
BauI CACGAG 1 cut(s) 1650
BbsI GAAGAC 2 cut(s) 58, 545
Bbv12I GWGCWC 1 cut(s) 1623
BbvI GCAGC 1 cut(s) 913
BccI CCATC 3 cut(s) 48, 1445, 1891
BceAI ACGGC 2 cut(s) 47, 1032
BciT130I CCWGG 1 cut(s) 95
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 1927
BfaI CTAG 2 cut(s) 1190, 1664
BfmI CTRYAG 2 cut(s) 1002, 1698
BfoI RGCGCY 2 cut(s) 9, 1463
BglII AGATCT 1 cut(s) 943
BisI GCNGC 4 cut(s) 231, 234, 600, 927
BlpI GCTNAGC 1 cut(s) 260
BlsI GCNGC 4 cut(s) 232, 235, 601, 928
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 250
BmeT110I CYCGRG 1 cut(s) 282
BmgT120I GGNCC 3 cut(s) 97, 240, 250
BmiI GGNNCC 1 cut(s) 1819
BmrFI CCNGG 1 cut(s) 95
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 7 cut(s) 259, 768, 1164, 1377, 1619, 1819, 1846
BmuI ACTGGG 1 cut(s) 326
BoxI GACNNNNGTC 1 cut(s) 1919
BpiI GAAGAC 2 cut(s) 58, 545
BpmI CTGGAG 1 cut(s) 849
Bpu1102I GCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 1120, 1391
BsaWI WCCGGW 1 cut(s) 1084
BsaXI ACNNNNNCTCC 2 cut(s) 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 237, 283, 393
Bse118I RCCGGY 2 cut(s) 113, 255
Bse1I ACTGG 1 cut(s) 332
Bse3DI GCAATG 3 cut(s) 412, 1095, 1590
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 1120, 1391
BseGI GGATG 8 cut(s) 509, 757, 771, 889, 1081, 1093, 1634, 1731
BseLI CCNNNNNNNGG 3 cut(s) 237, 283, 393
BseMI GCAATG 3 cut(s) 412, 1095, 1590
BseMII CTCAG 8 cut(s) 141, 274, 548, 774, 1254, 1479, 1521, 1938
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 4 cut(s) 61, 110, 143, 1682
BseXI GCAGC 1 cut(s) 913
BseYI CCCAGC 2 cut(s) 99, 878
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 98, 117, 241, 1673
BshNI GGYRCC 1 cut(s) 1817
BsiHKAI GWGCWC 1 cut(s) 1623
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 3 cut(s) 114, 256, 1085
BslFI GGGAC 1 cut(s) 1883
BslI CCNNNNNNNGG 3 cut(s) 237, 283, 393
BsmAI GTCTC 1 cut(s) 1927
BsmFI GGGAC 1 cut(s) 1883
BsmI GAATGC 1 cut(s) 1139
BsnI GGCC 4 cut(s) 98, 117, 241, 1673
BsoBI CYCGRG 1 cut(s) 282
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 1164, 1623
Bsp143I GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
Bsp1720I GCTNAGC 1 cut(s) 260
Bsp19I CCATGG 1 cut(s) 1120
BspANI GGCC 4 cut(s) 98, 117, 241, 1673
BspCNI CTCAG 8 cut(s) 140, 273, 547, 775, 1253, 1480, 1520, 1937
BspFNI CGCG 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 1819
BspPI GGATC 3 cut(s) 335, 437, 1111
BspQI GCTCTTC 2 cut(s) 841, 1169
BspT104I TTCGAA 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 1817
BsrDI GCAATG 3 cut(s) 412, 1095, 1590
BsrFI RCCGGY 2 cut(s) 113, 255
BsrI ACTGG 1 cut(s) 332
BssAI RCCGGY 2 cut(s) 113, 255
BssECI CCNNGG 2 cut(s) 1120, 1391
BssMI GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
BssSI CACGAG 1 cut(s) 1650
BssT1I CCWWGG 1 cut(s) 1120
Bst2BI CACGAG 1 cut(s) 1650
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 6 cut(s) 464, 544, 553, 740, 1003, 1445
Bst6I CTCTTC 4 cut(s) 485, 841, 1169, 1682
BstAPI GCANNNNNTGC 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 4 cut(s) 257, 511, 1231, 1537
BstDSI CCRYGG 2 cut(s) 1120, 1391
BstF5I GGATG 8 cut(s) 509, 757, 771, 889, 1081, 1093, 1634, 1731
BstFNI CGCG 1 cut(s) 484
BstH2I RGCGCY 2 cut(s) 9, 1463
BstHHI GCGC 2 cut(s) 8, 1462
BstKTI GATC 7 cut(s) 343, 366, 432, 946, 1119, 1543, 1828
BstMAI GTCTC 1 cut(s) 1927
BstMBI GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
BstMWI GCNNNNNNNGC 6 cut(s) 261, 722, 755, 824, 1466, 1702
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 1339
BstPAI GACNNNNGTC 1 cut(s) 1919
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 2 cut(s) 1002, 1698
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 1 cut(s) 913
BstV2I GAAGAC 2 cut(s) 58, 545
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
BsuRI GGCC 4 cut(s) 98, 117, 241, 1673
BtgI CCRYGG 2 cut(s) 1120, 1391
BtsCI GGATG 8 cut(s) 509, 757, 771, 889, 1081, 1093, 1634, 1731
BtsI GCAGTG 2 cut(s) 1621, 1805
BtsIMutI CAGTG 5 cut(s) 1019, 1450, 1528, 1621, 1805
Cac8I GCNNGC 4 cut(s) 257, 511, 1231, 1537
CfoI GCGC 2 cut(s) 8, 1462
Cfr10I RCCGGY 2 cut(s) 113, 255
Cfr13I GGNCC 3 cut(s) 97, 240, 250
Csp6I GTAC 2 cut(s) 1033, 1645
CviQI GTAC 2 cut(s) 1033, 1645
DpnI GATC 7 cut(s) 342, 365, 431, 945, 1118, 1542, 1827
DpnII GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
Eam1104I CTCTTC 4 cut(s) 485, 841, 1169, 1682
EarI CTCTTC 4 cut(s) 485, 841, 1169, 1682
EciI GGCGGA 2 cut(s) 242, 764
Eco130I CCWWGG 1 cut(s) 1120
Eco147I AGGCCT 1 cut(s) 1673
Eco24I GRGCYC 1 cut(s) 1164
Eco47I GGWCC 1 cut(s) 250
Eco47III AGCGCT 2 cut(s) 7, 1461
Eco57I CTGAAG 2 cut(s) 669, 1150
Eco88I CYCGRG 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 1120
EcoT22I ATGCAT 1 cut(s) 1634
EcoT38I GRGCYC 1 cut(s) 1164
ErhI CCWWGG 1 cut(s) 1120
FalI AAGNNNNNCTT 2 cut(s) 1449, 1481
FaqI GGGAC 1 cut(s) 1883
FauI CCCGC 4 cut(s) 26, 67, 709, 1343
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 231, 234, 600, 927
FokI GGATG 8 cut(s) 516, 764, 778, 896, 1088, 1100, 1641, 1738
FriOI GRGCYC 1 cut(s) 1164
Fsp4HI GCNGC 4 cut(s) 231, 234, 600, 927
FspBI CTAG 2 cut(s) 1190, 1664
GlaI GCGC 2 cut(s) 7, 1461
GluI GCNGC 4 cut(s) 231, 234, 600, 927
GsaI CCCAGC 2 cut(s) 103, 882
GsuI CTGGAG 1 cut(s) 849
HaeII RGCGCY 2 cut(s) 9, 1463
HaeIII GGCC 4 cut(s) 98, 117, 241, 1673
HapII CCGG 3 cut(s) 114, 256, 1085
HhaI GCGC 2 cut(s) 8, 1462
Hin6I GCGC 2 cut(s) 6, 1460
HinP1I GCGC 2 cut(s) 6, 1460
HindIII AAGCTT 1 cut(s) 1891
HinfI GANTC 4 cut(s) 891, 964, 1260, 1788
HpaII CCGG 3 cut(s) 114, 256, 1085
HphI GGTGA 3 cut(s) 97, 199, 848
Hpy166II GTNNAC 2 cut(s) 1046, 1413
Hpy188I TCNGA 6 cut(s) 130, 537, 1014, 1173, 1569, 1945
Hpy188III TCNNGA 3 cut(s) 395, 1148, 1474
Hpy8I GTNNAC 2 cut(s) 1046, 1413
Hpy99I CGWCG 1 cut(s) 1906
HpyCH4III ACNGT 6 cut(s) 464, 544, 553, 740, 1003, 1445
HpyCH4IV ACGT 2 cut(s) 322, 1401
HpyF10VI GCNNNNNNNGC 6 cut(s) 261, 722, 755, 824, 1466, 1702
HpySE526I ACGT 2 cut(s) 322, 1401
HspAI GCGC 2 cut(s) 6, 1460
KroI GCCGGC 1 cut(s) 255
KroNI GCCGGC 1 cut(s) 257
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
LguI GCTCTTC 2 cut(s) 841, 1169
LmnI GCTCC 3 cut(s) 87, 1353, 1940
Lsp1109I GCAGC 1 cut(s) 913
LweI GCATC 7 cut(s) 259, 768, 1164, 1377, 1619, 1819, 1846
MaeI CTAG 2 cut(s) 1190, 1664
MaeII ACGT 2 cut(s) 322, 1401
MaeIII GTNAC 5 cut(s) 547, 664, 1510, 1831, 1912
MalI GATC 7 cut(s) 342, 365, 431, 945, 1118, 1542, 1827
MboI GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
MfeI CAATTG 2 cut(s) 1039, 1614
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 2 cut(s) 1164, 1623
MluCI AATT 8 cut(s) 380, 451, 528, 556, 658, 1039, 1419, 1614
MmeI TCCRAC 3 cut(s) 585, 868, 1866
Mph1103I ATGCAT 1 cut(s) 1634
MroNI GCCGGC 1 cut(s) 255
MroXI GAANNNNTTC 1 cut(s) 1461
MseI TTAA 6 cut(s) 615, 711, 1026, 1220, 1734, 1949
MslI CAYNNNNRTG 2 cut(s) 1125, 1815
MspI CCGG 3 cut(s) 114, 256, 1085
MspR9I CCNGG 1 cut(s) 95
MunI CAATTG 2 cut(s) 1039, 1614
Mva1269I GAATGC 1 cut(s) 1139
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 6 cut(s) 261, 722, 755, 824, 1466, 1702
NaeI GCCGGC 1 cut(s) 257
NcoI CCATGG 1 cut(s) 1120
NdeII GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
NgoMIV GCCGGC 1 cut(s) 255
NlaIV GGNNCC 1 cut(s) 1819
NmeAIII GCCGAG 1 cut(s) 443
NmuCI GTSAC 2 cut(s) 547, 1912
NsiI ATGCAT 1 cut(s) 1634
NspI RCATGY 1 cut(s) 1339
NspV TTCGAA 1 cut(s) 488
PceI AGGCCT 1 cut(s) 1673
PciI ACATGT 1 cut(s) 1335
PciSI GCTCTTC 2 cut(s) 841, 1169
PctI GAATGC 1 cut(s) 1139
PdiI GCCGGC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 1461
PfeI GAWTC 4 cut(s) 891, 964, 1260, 1788
PkrI GCNGC 4 cut(s) 232, 235, 601, 928
PscI ACATGT 1 cut(s) 1335
PshAI GACNNNNGTC 1 cut(s) 1919
PshBI ATTAAT 1 cut(s) 1734
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 93
PspFI CCCAGC 2 cut(s) 99, 878
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 1819
PspPI GGNCC 3 cut(s) 97, 240, 250
PsuI RGATCY 1 cut(s) 943
RsaI GTAC 2 cut(s) 1034, 1646
RsaNI GTAC 2 cut(s) 1033, 1645
RseI CAYNNNNRTG 2 cut(s) 1125, 1815
SapI GCTCTTC 2 cut(s) 841, 1169
SaqAI TTAA 6 cut(s) 615, 711, 1026, 1220, 1734, 1949
SatI GCNGC 4 cut(s) 231, 234, 600, 927
Sau3AI GATC 7 cut(s) 340, 363, 429, 943, 1116, 1540, 1825
Sau96I GGNCC 3 cut(s) 97, 240, 250
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 1164, 1623
SfaNI GCATC 7 cut(s) 259, 768, 1164, 1377, 1619, 1819, 1846
SfcI CTRYAG 2 cut(s) 1002, 1698
SfuI TTCGAA 1 cut(s) 488
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 1125, 1815
Sse9I AATT 8 cut(s) 380, 451, 528, 556, 658, 1039, 1419, 1614
SseBI AGGCCT 1 cut(s) 1673
SspMI CTAG 2 cut(s) 1190, 1664
StuI AGGCCT 1 cut(s) 1673
StyD4I CCNGG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 1120
TaaI ACNGT 6 cut(s) 464, 544, 553, 740, 1003, 1445
TaiI ACGT 2 cut(s) 325, 1404
TaqI TCGA 6 cut(s) 156, 488, 771, 894, 1131, 1263
TasI AATT 8 cut(s) 380, 451, 528, 556, 658, 1039, 1419, 1614
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 3 cut(s) 233, 236, 602
TfiI GAWTC 4 cut(s) 891, 964, 1260, 1788
Tru1I TTAA 6 cut(s) 615, 711, 1026, 1220, 1734, 1949
Tru9I TTAA 6 cut(s) 615, 711, 1026, 1220, 1734, 1949
TscAI CASTG 5 cut(s) 1026, 1450, 1528, 1628, 1812
TseFI GTSAC 2 cut(s) 547, 1912
TseI GCWGC 1 cut(s) 926
Tsp45I GTSAC 2 cut(s) 547, 1912
TspGWI ACGGA 1 cut(s) 1408
TspRI CASTG 5 cut(s) 1026, 1450, 1528, 1628, 1812
VpaK11BI GGWCC 1 cut(s) 250
VspI ATTAAT 1 cut(s) 1734
XapI RAATTY 2 cut(s) 451, 528
XceI RCATGY 1 cut(s) 1339
XmnI GAANNNNTTC 1 cut(s) 1461
XspI CTAG 2 cut(s) 1190, 1664
Zsp2I ATGCAT 1 cut(s) 1634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.