FvH4_5g15171

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009027
Physical Location & Seq
Forward (+)
8570201 .. 8572645
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15171.t1

Sequence Viewer

Length: 741 bp
ATGGGTTCCAAGGATCCTGCTCCGGTGGTTGACAAGGTGAATGGGGCAACGCCGGCGCCGGTGGACCAGGCTTGTCCTGGCAAAAAGATTCAGGGAAAAGTTCCTAAGAGAATCCACAAGGCTGTGAGGGAGAAACAGAAGCGTGAGCATTTAAATGATCTTTTTCTTGAGCTGTCTGACGCTCTTGGACTGAATCAGCAGAACACTGGAAAGGCCACTATACTGTGTGAAGCTACTCGACTACTAAAGGATTTGCTTGGTCAAATCGAATCCTTAAGAAGCGAGAATGTATCCTTGTTGTCTGAATCTAACTATATGACTGTTGAGAAGAATGAACTCCGAGAGGATACTTCTGCCTTGGAAACTCAGATTGAAAGATTGCAAAGTGAGCTACAAGAGAGGGTGTTCCAGTCGAAACCTGACCTTAATGTACCACCTGTAGAAGTGGCAGCCCATTTTACCGGAAATTGTCTAGGATTGCCTGCTCAAGAATCAGCTTTGCAACAATCACCTGCTGTATTTGTCATGCCCCTCCTCCCTGATCTCCAAACTTATCCACCAGCAGATTCCGCCCACCTCACATCCAAGACCAACTCAAATGTAAGCAAACCACATGCTAGGTATCCGACCTCGACGGATTGTTGGCCATTTCAACTTCTCGGTGAGCAACCAGCAGCAGGCAAAGACGCTCGACAGAATGATCACAGTGCCAGCGGATATAATGGCCATGTTAACATATAA

Protein Analysis

247

Amino Acids

26.92

Weight (kDa)

5.99

Isoelectric Point (pI)

58.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_IRO3 PF23177 33 - 110 5.5e-26 Iron-related transcription factor 3 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 55
AciI CCGC 2 cut(s) 570, 714
AclWI GGATC 2 cut(s) 8, 21
AcoI YGGCCR 2 cut(s) 644, 724
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 677
AflII CTTAAG 1 cut(s) 274
AgsI TTSAA 2 cut(s) 374, 653
AjnI CCWGG 2 cut(s) 66, 76
AluBI AGCT 4 cut(s) 172, 233, 391, 497
AluI AGCT 4 cut(s) 172, 233, 391, 497
AlwI GGATC 2 cut(s) 8, 21
AoxI GGCC 3 cut(s) 213, 644, 724
ApeKI GCWGC 2 cut(s) 449, 674
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 49, 501, 674
AvaII GGWCC 1 cut(s) 64
BalI TGGCCA 2 cut(s) 646, 726
BamHI GGATCC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 55
BbvI GCAGC 2 cut(s) 461, 686
BciT130I CCWGG 2 cut(s) 68, 78
BciVI GTATCC 3 cut(s) 301, 340, 633
BclI TGATCA 1 cut(s) 700
BfaI CTAG 2 cut(s) 473, 618
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 3 cut(s) 301, 340, 633
BisI GCNGC 2 cut(s) 450, 675
BlsI GCNGC 2 cut(s) 451, 676
Bme1390I CCNGG 2 cut(s) 68, 78
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 7, 15, 57
BmrFI CCNGG 2 cut(s) 68, 78
BpuEI CTTGAG 2 cut(s) 188, 471
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 9, 357
BsaWI WCCGGW 2 cut(s) 22, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 677
Bse118I RCCGGY 2 cut(s) 52, 58
Bse1I ACTGG 2 cut(s) 211, 409
BseBI CCWGG 2 cut(s) 68, 78
BseDI CCNNGG 2 cut(s) 9, 357
BseGI GGATG 1 cut(s) 581
BseLI CCNNNNNNNGG 1 cut(s) 677
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 2 cut(s) 211, 409
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 2 cut(s) 461, 686
BshFI GGCC 3 cut(s) 215, 646, 726
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 4 cut(s) 23, 53, 59, 462
BslI CCNNNNNNNGG 1 cut(s) 677
BsnI GGCC 3 cut(s) 215, 646, 726
Bsp143I GATC 4 cut(s) 13, 157, 541, 700
BspACI CCGC 2 cut(s) 570, 714
BspANI GGCC 3 cut(s) 215, 646, 726
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 3 cut(s) 7, 15, 57
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 2 cut(s) 8, 21
BspT107I GGYRCC 1 cut(s) 55
BspTI CTTAAG 1 cut(s) 274
BsrFI RCCGGY 2 cut(s) 52, 58
BsrI ACTGG 2 cut(s) 211, 409
BssAI RCCGGY 2 cut(s) 52, 58
BssECI CCNNGG 2 cut(s) 9, 357
BssMI GATC 4 cut(s) 13, 157, 541, 700
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 2 cut(s) 9, 357
Bst2UI CCWGG 2 cut(s) 68, 78
Bst4CI ACNGT 3 cut(s) 225, 322, 707
BstACI GRCGYC 1 cut(s) 56
BstAFI CTTAAG 1 cut(s) 274
BstC8I GCNNGC 4 cut(s) 54, 483, 679, 712
BstDEI CTNAG 2 cut(s) 105, 366
BstF5I GGATG 1 cut(s) 581
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 4 cut(s) 16, 160, 544, 703
BstMBI GATC 4 cut(s) 13, 157, 541, 700
BstMWI GCNNNNNNNGC 3 cut(s) 53, 388, 569
BstNI CCWGG 2 cut(s) 68, 78
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 66, 76
BstSFI CTRYAG 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 461, 686
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuI GTATCC 3 cut(s) 301, 340, 633
BsuRI GGCC 3 cut(s) 215, 646, 726
BtsCI GGATG 1 cut(s) 581
BtsIMutI CAGTG 2 cut(s) 204, 712
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 4 cut(s) 54, 483, 679, 712
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 2 cut(s) 52, 58
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 2 cut(s) 188, 695
Csp6I GTAC 1 cut(s) 431
CviAII CATG 3 cut(s) 526, 614, 728
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 2 cut(s) 105, 366
DinI GGCGCC 1 cut(s) 57
DpnI GATC 4 cut(s) 15, 159, 543, 702
DpnII GATC 4 cut(s) 13, 157, 541, 700
DraI TTTAAA 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 644, 724
EciI GGCGGA 1 cut(s) 559
Eco130I CCWWGG 2 cut(s) 9, 357
Eco47I GGWCC 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 66, 76
EcoT14I CCWWGG 2 cut(s) 9, 357
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 9, 357
FaeI CATG 3 cut(s) 529, 617, 731
FaiI YATR 9 cut(s) 221, 315, 317, 527, 615, 720, 729, 737, 739
FatI CATG 3 cut(s) 525, 613, 727
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 2 cut(s) 450, 675
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 2 cut(s) 450, 675
FspBI CTAG 2 cut(s) 473, 618
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 450, 675
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 3 cut(s) 215, 646, 726
HapII CCGG 4 cut(s) 23, 53, 59, 462
HgaI GACGC 2 cut(s) 188, 695
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 3 cut(s) 529, 617, 731
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 2 cut(s) 31, 733
HindII GTYRAC 2 cut(s) 31, 733
HinfI GANTC 7 cut(s) 88, 111, 193, 269, 305, 491, 566
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 4 cut(s) 23, 53, 59, 462
HphI GGTGA 3 cut(s) 49, 501, 674
Hpy166II GTNNAC 3 cut(s) 31, 64, 733
Hpy188I TCNGA 5 cut(s) 178, 304, 341, 369, 627
Hpy188III TCNNGA 2 cut(s) 167, 488
Hpy8I GTNNAC 3 cut(s) 31, 64, 733
Hpy99I CGWCG 1 cut(s) 637
HpyCH4III ACNGT 3 cut(s) 225, 322, 707
HpyCH4V TGCA 2 cut(s) 382, 502
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 388, 569
HpyF3I CTNAG 2 cut(s) 105, 366
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 3 cut(s) 529, 617, 731
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 700
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 4 cut(s) 13, 157, 541, 700
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 2 cut(s) 461, 686
MaeI CTAG 2 cut(s) 473, 618
MalI GATC 4 cut(s) 15, 159, 543, 702
MboI GATC 4 cut(s) 13, 157, 541, 700
MboII GAAGA 1 cut(s) 340
MflI RGATCY 1 cut(s) 13
MlsI TGGCCA 2 cut(s) 646, 726
MluCI AATT 1 cut(s) 466
MluNI TGGCCA 2 cut(s) 646, 726
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 7 cut(s) 120, 337, 393, 542, 545, 587, 640
Mox20I TGGCCA 2 cut(s) 646, 726
MreI CGCCGGCG 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 52
MscI TGGCCA 2 cut(s) 646, 726
MseI TTAA 4 cut(s) 152, 275, 426, 732
MslI CAYNNNNRTG 1 cut(s) 153
Msp20I TGGCCA 2 cut(s) 646, 726
MspA1I CMGCKG 1 cut(s) 714
MspCI CTTAAG 1 cut(s) 274
MspI CCGG 4 cut(s) 23, 53, 59, 462
MspR9I CCNGG 2 cut(s) 68, 78
MvaI CCWGG 2 cut(s) 68, 78
MwoI GCNNNNNNNGC 3 cut(s) 53, 388, 569
NaeI GCCGGC 1 cut(s) 54
NarI GGCGCC 1 cut(s) 56
NdeII GATC 4 cut(s) 13, 157, 541, 700
NgoMIV GCCGGC 1 cut(s) 52
NlaIII CATG 3 cut(s) 529, 617, 731
NlaIV GGNNCC 3 cut(s) 7, 15, 57
NspI RCATGY 1 cut(s) 617
PaqCI CACCTGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 54
PfeI GAWTC 7 cut(s) 88, 111, 193, 269, 305, 491, 566
PkrI GCNGC 2 cut(s) 451, 676
PluTI GGCGCC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 66, 76
PspGI CCWGG 2 cut(s) 66, 76
PspN4I GGNNCC 3 cut(s) 7, 15, 57
PspPI GGNCC 1 cut(s) 64
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 4 cut(s) 152, 275, 426, 732
SatI GCNGC 2 cut(s) 450, 675
Sau3AI GATC 4 cut(s) 13, 157, 541, 700
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 68, 78
SfcI CTRYAG 1 cut(s) 438
SfoI GGCGCC 1 cut(s) 57
SgrAI CRCCGGYG 2 cut(s) 52, 58
SinI GGWCC 1 cut(s) 64
SmiI ATTTAAAT 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 3 cut(s) 167, 274, 486
SmoI CTYRAG 3 cut(s) 167, 274, 486
Sse9I AATT 1 cut(s) 466
SsiI CCGC 2 cut(s) 570, 714
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 2 cut(s) 473, 618
StyD4I CCNGG 2 cut(s) 66, 76
StyI CCWWGG 2 cut(s) 9, 357
SwaI ATTTAAAT 1 cut(s) 153
TaaI ACNGT 3 cut(s) 225, 322, 707
TaqI TCGA 5 cut(s) 238, 267, 413, 632, 691
TasI AATT 1 cut(s) 466
TfiI GAWTC 7 cut(s) 88, 111, 193, 269, 305, 491, 566
Tru1I TTAA 4 cut(s) 152, 275, 426, 732
Tru9I TTAA 4 cut(s) 152, 275, 426, 732
TscAI CASTG 2 cut(s) 211, 712
TseI GCWGC 2 cut(s) 449, 674
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 2 cut(s) 211, 712
Vha464I CTTAAG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 64
XceI RCATGY 1 cut(s) 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 2 cut(s) 473, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.