RchiOBHm_Chr7g0181001

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009027
Physical Location & Seq
Reverse (-)
2713095 .. 2715710
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16143

Sequence Viewer

Length: 750 bp
ATGGGTTCCAAGGATCCTGCTCCGGTGGTTGACAAGGTTAATGAGGCAACGCAGCCGGTGGACCGGGTTTGTCCTGGCAAAAAGATTCAGGGGAAAATGCCTAAGAGAATCCACAAGGCTGTGAGGGAGAAACAGAAGCGTGAGCATTTGAATGATCTTTTTCTTGATCTGTCTGATGCTCTCGAACTGAATCAGCAGAACACTGGAAAGGCCACTATACTTTGTGAAGCTACTCGACTATTAAAGGATTTGCTTGGTCAAATCGAATCCTTGAGAAACGAGAATGTATCCTTGTTGTCCGAATCTAACTATATGACTGTTGAGAAGAATGAGCTCCGAGAGGATACTTCTGCCTTAGAAACTCAGATTGAAAGATTGCAAAGTGAGCTACAAGAGCGGGTTCGCCAGTCTAAACCTGACCTTAATGTACCACCTGTAGAAGTTAGACAAGAAGTGGCATCCCATTTTCCTGGAAATTGTGTCGGATTTCCTGCTCAAGAATCAGGTTTGCAACAAGCACCTGCTGTCTTTGTCATGCCCCTCCACCCTGATCTCCAAACTTATCCACCGGCAGATTCCGCCCACCTTACATCTAAGATCAACTCAAATGTGAGCAAACCACACCCTAGGTATCCAACCTCGGTGGATTCTTGGCCATTTCAACTTCTCAGTGAGCAACCAGCAGCAGGCAAAGACTCTCGACAGAATGGTCACAGTACCAGCGGATATAATGGCCATGATAACATGTAA

Protein Analysis

249

Amino Acids

27.74

Weight (kDa)

6.08

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_IRO3 PF23177 32 - 109 7.5e-26 Iron-related transcription factor 3 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
AasI GACNNNNNNGTC 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 529
AccBSI CCGCTC 1 cut(s) 397
AciI CCGC 3 cut(s) 397, 579, 723
AclWI GGATC 2 cut(s) 8, 21
AcoI YGGCCR 2 cut(s) 653, 733
AfaI GTAC 2 cut(s) 429, 718
AfiI CCNNNNNNNGG 1 cut(s) 686
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 3 cut(s) 151, 371, 662
AjnI CCWGG 2 cut(s) 73, 469
AluBI AGCT 3 cut(s) 230, 334, 388
AluI AGCT 3 cut(s) 230, 334, 388
Alw21I GWGCWC 1 cut(s) 336
AlwI GGATC 2 cut(s) 8, 21
AoxI GGCC 3 cut(s) 210, 653, 733
ApeKI GCWGC 2 cut(s) 52, 683
AspA2I CCTAGG 1 cut(s) 626
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 61
AvrII CCTAGG 1 cut(s) 626
BalI TGGCCA 2 cut(s) 655, 735
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 64, 695
BciT130I CCWGG 2 cut(s) 75, 471
BciVI GTATCC 3 cut(s) 298, 337, 642
BcnI CCSGG 1 cut(s) 65
BfaI CTAG 1 cut(s) 627
BfmI CTRYAG 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 529
BfuI GTATCC 3 cut(s) 298, 337, 642
BisI GCNGC 2 cut(s) 53, 684
BlnI CCTAGG 1 cut(s) 626
BlsI GCNGC 2 cut(s) 54, 685
Bme1390I CCNGG 3 cut(s) 65, 75, 471
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 2 cut(s) 7, 15
BmrFI CCNGG 3 cut(s) 65, 75, 471
BmsI GCATC 2 cut(s) 166, 467
BpuEI CTTGAG 2 cut(s) 292, 480
BpuMI CCSGG 1 cut(s) 65
BsaJI CCNNGG 3 cut(s) 9, 626, 639
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse118I RCCGGY 2 cut(s) 55, 568
Bse1I ACTGG 2 cut(s) 208, 406
BseBI CCWGG 2 cut(s) 75, 471
BseDI CCNNGG 3 cut(s) 9, 626, 639
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMII CTCAG 2 cut(s) 377, 682
BseNI ACTGG 2 cut(s) 208, 406
BseXI GCAGC 2 cut(s) 64, 695
BshFI GGCC 3 cut(s) 212, 655, 735
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 4 cut(s) 23, 56, 64, 569
BslI CCNNNNNNNGG 1 cut(s) 686
BsnI GGCC 3 cut(s) 212, 655, 735
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 5 cut(s) 13, 154, 166, 550, 597
BspACI CCGC 3 cut(s) 397, 579, 723
BspANI GGCC 3 cut(s) 212, 655, 735
BspCNI CTCAG 2 cut(s) 376, 681
BspLI GGNNCC 2 cut(s) 7, 15
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 8, 21
BsrBI CCGCTC 1 cut(s) 397
BsrFI RCCGGY 2 cut(s) 55, 568
BsrI ACTGG 2 cut(s) 208, 406
BssAI RCCGGY 2 cut(s) 55, 568
BssECI CCNNGG 3 cut(s) 9, 626, 639
BssMI GATC 5 cut(s) 13, 154, 166, 550, 597
BssT1I CCWWGG 2 cut(s) 9, 626
Bst2UI CCWGG 2 cut(s) 75, 471
Bst4CI ACNGT 2 cut(s) 319, 716
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 5 cut(s) 102, 355, 363, 594, 668
BstF5I GGATG 1 cut(s) 458
BstKTI GATC 5 cut(s) 16, 157, 169, 553, 600
BstMBI GATC 5 cut(s) 13, 154, 166, 550, 597
BstMWI GCNNNNNNNGC 3 cut(s) 385, 394, 578
BstNI CCWGG 2 cut(s) 75, 471
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 3 cut(s) 63, 73, 469
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 64, 695
BstX2I RGATCY 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 1 cut(s) 13
BsuI GTATCC 3 cut(s) 298, 337, 642
BsuRI GGCC 3 cut(s) 212, 655, 735
BtsCI GGATG 1 cut(s) 458
BtsIMutI CAGTG 2 cut(s) 201, 676
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 688
Cfr10I RCCGGY 2 cut(s) 55, 568
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 428, 717
CspCI CAANNNNNGTGG 2 cut(s) 624, 659
CviAII CATG 3 cut(s) 535, 737, 745
CviJI RGCY 8 cut(s) 55, 119, 212, 230, 334, 388, 655, 735
CviKI_1 RGCY 8 cut(s) 55, 119, 212, 230, 334, 388, 655, 735
CviQI GTAC 2 cut(s) 428, 717
DdeI CTNAG 5 cut(s) 102, 355, 363, 594, 668
DpnI GATC 5 cut(s) 15, 156, 168, 552, 599
DpnII GATC 5 cut(s) 13, 154, 166, 550, 597
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 653, 733
EciI GGCGGA 1 cut(s) 568
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 9, 626
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 73, 469
EcoT14I CCWWGG 2 cut(s) 9, 626
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 2 cut(s) 9, 626
FaeI CATG 3 cut(s) 538, 740, 748
FaiI YATR 7 cut(s) 218, 312, 314, 536, 729, 738, 746
FatI CATG 3 cut(s) 534, 736, 744
FauI CCCGC 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 53, 684
FokI GGATG 1 cut(s) 445
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 2 cut(s) 53, 684
FspBI CTAG 1 cut(s) 627
GluI GCNGC 2 cut(s) 53, 684
HaeIII GGCC 3 cut(s) 212, 655, 735
HapII CCGG 4 cut(s) 23, 56, 64, 569
Hin1II CATG 3 cut(s) 538, 740, 748
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 9 cut(s) 85, 108, 190, 266, 302, 500, 575, 647, 695
HpaII CCGG 4 cut(s) 23, 56, 64, 569
Hpy166II GTNNAC 2 cut(s) 31, 61
Hpy188I TCNGA 5 cut(s) 175, 301, 338, 366, 485
Hpy188III TCNNGA 4 cut(s) 164, 182, 497, 699
Hpy8I GTNNAC 2 cut(s) 31, 61
HpyCH4III ACNGT 2 cut(s) 319, 716
HpyCH4V TGCA 2 cut(s) 379, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 394, 578
HpyF3I CTNAG 5 cut(s) 102, 355, 363, 594, 668
Hsp92II CATG 3 cut(s) 538, 740, 748
Kzo9I GATC 5 cut(s) 13, 154, 166, 550, 597
LmnI GCTCC 2 cut(s) 25, 339
Lsp1109I GCAGC 2 cut(s) 64, 695
LweI GCATC 2 cut(s) 166, 467
MaeI CTAG 1 cut(s) 627
MaeIII GTNAC 1 cut(s) 710
MalI GATC 5 cut(s) 15, 156, 168, 552, 599
MbiI CCGCTC 1 cut(s) 397
MboI GATC 5 cut(s) 13, 154, 166, 550, 597
MboII GAAGA 1 cut(s) 337
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 2 cut(s) 655, 735
MluCI AATT 1 cut(s) 475
MluNI TGGCCA 2 cut(s) 655, 735
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 2 cut(s) 463, 659
MnlI CCTC 5 cut(s) 37, 117, 334, 551, 649
Mox20I TGGCCA 2 cut(s) 655, 735
MscI TGGCCA 2 cut(s) 655, 735
MseI TTAA 3 cut(s) 39, 242, 423
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 2 cut(s) 655, 735
MspA1I CMGCKG 1 cut(s) 723
MspI CCGG 4 cut(s) 23, 56, 64, 569
MspR9I CCNGG 3 cut(s) 65, 75, 471
MvaI CCWGG 2 cut(s) 75, 471
MwoI GCNNNNNNNGC 3 cut(s) 385, 394, 578
NciI CCSGG 1 cut(s) 65
NdeII GATC 5 cut(s) 13, 154, 166, 550, 597
NlaIII CATG 3 cut(s) 538, 740, 748
NlaIV GGNNCC 2 cut(s) 7, 15
NmuCI GTSAC 1 cut(s) 710
NspI RCATGY 1 cut(s) 748
PaqCI CACCTGC 1 cut(s) 529
PciI ACATGT 1 cut(s) 744
PfeI GAWTC 8 cut(s) 85, 108, 190, 266, 302, 500, 575, 647
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 2 cut(s) 54, 685
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
PscI ACATGT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 73, 469
PspGI CCWGG 2 cut(s) 73, 469
PspN4I GGNNCC 2 cut(s) 7, 15
PspPI GGNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 2 cut(s) 429, 718
RsaNI GTAC 2 cut(s) 428, 717
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 3 cut(s) 39, 242, 423
SatI GCNGC 2 cut(s) 53, 684
Sau3AI GATC 5 cut(s) 13, 154, 166, 550, 597
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 3 cut(s) 65, 75, 471
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 166, 467
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 271, 495
SmoI CTYRAG 2 cut(s) 271, 495
Sse9I AATT 1 cut(s) 475
SsiI CCGC 3 cut(s) 397, 579, 723
SspMI CTAG 1 cut(s) 627
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 3 cut(s) 63, 73, 469
StyI CCWWGG 2 cut(s) 9, 626
TaaI ACNGT 2 cut(s) 319, 716
TaqI TCGA 4 cut(s) 183, 235, 264, 700
TasI AATT 1 cut(s) 475
TfiI GAWTC 8 cut(s) 85, 108, 190, 266, 302, 500, 575, 647
Tru1I TTAA 3 cut(s) 39, 242, 423
Tru9I TTAA 3 cut(s) 39, 242, 423
TscAI CASTG 2 cut(s) 208, 676
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 2 cut(s) 52, 683
Tsp45I GTSAC 1 cut(s) 710
TspRI CASTG 2 cut(s) 208, 676
VpaK11BI GGWCC 1 cut(s) 61
XceI RCATGY 1 cut(s) 748
XmaJI CCTAGG 1 cut(s) 626
XspI CTAG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.