Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009027

Physical Location & Seq

Genomic Coordinates Reverse (-)

28807081 .. 28809980

Gene Structure 2900 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1198000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 741 bp

ATGGGTTCACCGGATCCTTTGCCGGCAGCTGAAGAGGGTAATGTGGCAACGGAGGCATCAGTGCGCCGGGCTTGTCCTGGAATAAAGAAACAGGGGAAAATTCCTAAGAGAATCCGCAAGGCTGAGAGGGAGAGACGGAAGCGTGAGCAGTTTAACGAGCTTTTTCTTGGGCTGGCTGATGCCCTTGAACTGAATGAGCAGAACAGTGGAAAGGCCTCCATAATAAGTGAAGCTACTCGACTGTTAAAGGACTTGTGTGGTCAGATTGAATGCTTACAGAAGGAAAATGCATCCCTGTTGTCTGAATCGAACTATATGACTCTCGAGAAGAATGAGCTGAGAGACGATAACTCTGCCTTAGAAACTCAGATTGAGAAATTGCAAAGTGAGATACAAGAGAGGGTGGCACAGTCTAAACCGAACCTGAATGCACCACCTCGAGGAGAATTGAGACCAGAAGTTCCCTCCCATTTTACCGGAAATTGCATCAGTTTGCCCACTCAAGAACCCAGCTTGCAGCAAGCACCTGCTGTCTTTGTTATGCCCTTCTGCCCTGATCTCCAAGCTTATCCACTGCCGGATGCCACCCACCCCACATCTAACACCACCTCACATGTGAGCAAACCGCATGCTAGGTATCCGACCTCGGTGGATTCTTGGCCGTTCCAACTTCTCGGCGAGAAAACAACCGCAGGCAAAGGATTTCGACAAAGTGGTAAAAGCGACCCTAGTAATATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006873 GO:0006875 GO:0006879 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009987 GO:0009991 GO:0010106 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019725 GO:0030003 GO:0031323 GO:0031326 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042592 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046916 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0071496 GO:0080090 GO:0098771 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

247

Amino Acids

27.17

Weight (kDa)

6.46

Isoelectric Point (pI)

60.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_IRO3	PF23177	33 - 110	3.9e-20	Iron-related transcription factor 3 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47640 AT3G47640 AT3G47640
fragaria_vesca	FvH4_5g15171 FvH4_5g15171 FvH4_5g15171
malus_domestica	MD06G1191600.v1.1 MD14G1198000.v1.1
prunus_persica	Prupe.5G190100_v2.0.a1
pyrus_communis	pycom06g16970 pycom14g16440
rosa_chinensis	RchiOBHm_Chr7g0181001
rosa_multiflora	Rmu_co8331139.1_g000001
rosa_roxburghii	Rroxscaffold_3G00272350
rosa_rugosa	Rorug06G0438300 Rorug06G0438300 Rorug06G0438400
rosa_samantha	Rh7AG039600 Rh7BG039000 Rh7CG040800 Rh7DG039200
rosa_wichuraiana	Rw7G003260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	535
AbsI	CCTCGAGG	1 cut(s)	438
Acc36I	ACCTGC	1 cut(s)	535
AciI	CCGC	3 cut(s)	115, 626, 690
AclWI	GGATC	2 cut(s)	8, 21
AcoI	YGGCCR	1 cut(s)	659
AcsI	RAATTY	1 cut(s)	99
AcuI	CTGAAG	1 cut(s)	51
AfiI	CCNNNNNNNGG	1 cut(s)	440
AflIII	ACRYGT	1 cut(s)	613
AgsI	TTSAA	2 cut(s)	188, 269
AjnI	CCWGG	1 cut(s)	76
AluBI	AGCT	6 cut(s)	29, 160, 233, 337, 513, 566
AluI	AGCT	6 cut(s)	29, 160, 233, 337, 513, 566
Alw26I	GTCTC	3 cut(s)	127, 336, 445
AlwI	GGATC	2 cut(s)	8, 21
Ama87I	CYCGRG	2 cut(s)	323, 438
AoxI	GGCC	2 cut(s)	213, 659
ApeKI	GCWGC	2 cut(s)	26, 517
ApoI	RAATTY	1 cut(s)	99
AspLEI	GCGC	1 cut(s)	66
AsuC2I	CCSGG	1 cut(s)	68
AvaI	CYCGRG	2 cut(s)	323, 438
BamHI	GGATCC	1 cut(s)	13
BbvI	GCAGC	2 cut(s)	38, 529
BceAI	ACGGC	1 cut(s)	646
BcgI	CGANNNNNNTGC	2 cut(s)	335, 369
BciT130I	CCWGG	1 cut(s)	78
BciVI	GTATCC	1 cut(s)	648
BcnI	CCSGG	1 cut(s)	68
BcoDI	GTCTC	3 cut(s)	127, 336, 445
BfaI	CTAG	2 cut(s)	633, 729
BfuAI	ACCTGC	1 cut(s)	535
BfuI	GTATCC	1 cut(s)	648
BisI	GCNGC	2 cut(s)	27, 518
BlsI	GCNGC	2 cut(s)	28, 519
Bme1390I	CCNGG	2 cut(s)	68, 78
BmeT110I	CYCGRG	2 cut(s)	323, 438
BmiI	GGNNCC	1 cut(s)	15
BmrFI	CCNGG	2 cut(s)	68, 78
BmsI	GCATC	5 cut(s)	65, 169, 299, 495, 571
BpuEI	CTTGAG	1 cut(s)	486
BpuMI	CCSGG	1 cut(s)	68
BsaI	GGTCTC	1 cut(s)	445
BsaJI	CCNNGG	1 cut(s)	645
BsaWI	WCCGGW	2 cut(s)	10, 476
Bsc4I	CCNNNNNNNGG	1 cut(s)	440
Bse118I	RCCGGY	1 cut(s)	22
BseBI	CCWGG	1 cut(s)	78
BseDI	CCNNGG	1 cut(s)	645
BseGI	GGATG	2 cut(s)	290, 586
BseLI	CCNNNNNNNGG	1 cut(s)	440
BseMII	CTCAG	3 cut(s)	114, 329, 380
BseRI	GAGGAG	1 cut(s)	456
BseXI	GCAGC	2 cut(s)	38, 529
BseYI	CCCAGC	1 cut(s)	509
BshFI	GGCC	2 cut(s)	215, 661
BsiHKCI	CYCGRG	2 cut(s)	323, 438
BsiSI	CCGG	5 cut(s)	11, 23, 67, 477, 578
BslI	CCNNNNNNNGG	1 cut(s)	440
BsmAI	GTCTC	3 cut(s)	127, 336, 445
BsmBI	CGTCTC	2 cut(s)	127, 336
BsmI	GAATGC	2 cut(s)	275, 433
BsnI	GGCC	2 cut(s)	215, 661
Bso31I	GGTCTC	1 cut(s)	445
BsoBI	CYCGRG	2 cut(s)	323, 438
Bsp143I	GATC	2 cut(s)	13, 556
BspACI	CCGC	3 cut(s)	115, 626, 690
BspANI	GGCC	2 cut(s)	215, 661
BspCNI	CTCAG	3 cut(s)	115, 330, 379
BspLI	GGNNCC	1 cut(s)	15
BspMI	ACCTGC	1 cut(s)	535
BspPI	GGATC	2 cut(s)	8, 21
BspTNI	GGTCTC	1 cut(s)	445
BsrFI	RCCGGY	1 cut(s)	22
BssAI	RCCGGY	1 cut(s)	22
BssECI	CCNNGG	1 cut(s)	645
BssMI	GATC	2 cut(s)	13, 556
Bst2UI	CCWGG	1 cut(s)	78
Bst4CI	ACNGT	3 cut(s)	206, 243, 411
Bst6I	CTCTTC	1 cut(s)	27
BstC8I	GCNNGC	6 cut(s)	24, 174, 515, 522, 630, 694
BstDEI	CTNAG	5 cut(s)	105, 123, 338, 358, 366
BstF5I	GGATG	2 cut(s)	290, 586
BstHHI	GCGC	1 cut(s)	66
BstKTI	GATC	2 cut(s)	16, 559
BstMAI	GTCTC	3 cut(s)	127, 336, 445
BstMBI	GATC	2 cut(s)	13, 556
BstMWI	GCNNNNNNNGC	1 cut(s)	53
BstNI	CCWGG	1 cut(s)	78
BstNSI	RCATGY	2 cut(s)	617, 632
BstSCI	CCNGG	2 cut(s)	66, 76
BstV1I	GCAGC	2 cut(s)	38, 529
BstX2I	RGATCY	1 cut(s)	13
BstYI	RGATCY	1 cut(s)	13
BsuI	GTATCC	1 cut(s)	648
BsuRI	GGCC	2 cut(s)	215, 661
BtsCI	GGATG	2 cut(s)	290, 586
BtsI	GCAGTG	1 cut(s)	572
BtsIMutI	CAGTG	3 cut(s)	66, 211, 572
BveI	ACCTGC	1 cut(s)	535
Cac8I	GCNNGC	6 cut(s)	24, 174, 515, 522, 630, 694
CfoI	GCGC	1 cut(s)	66
Cfr10I	RCCGGY	1 cut(s)	22
CviAII	CATG	2 cut(s)	614, 629
DdeI	CTNAG	5 cut(s)	105, 123, 338, 358, 366
DpnI	GATC	2 cut(s)	15, 558
DpnII	GATC	2 cut(s)	13, 556
EaeI	YGGCCR	1 cut(s)	659
Eam1104I	CTCTTC	1 cut(s)	27
EarI	CTCTTC	1 cut(s)	27
Eco147I	AGGCCT	1 cut(s)	215
Eco31I	GGTCTC	1 cut(s)	445
Eco57I	CTGAAG	1 cut(s)	51
Eco88I	CYCGRG	2 cut(s)	323, 438
EcoRII	CCWGG	1 cut(s)	76
EcoT22I	ATGCAT	1 cut(s)	292
Esp3I	CGTCTC	2 cut(s)	127, 336
FaeI	CATG	2 cut(s)	617, 632
FaiI	YATR	7 cut(s)	221, 315, 317, 542, 615, 630, 737
FatI	CATG	2 cut(s)	613, 628
Fnu4HI	GCNGC	2 cut(s)	27, 518
FokI	GGATG	2 cut(s)	277, 593
Fsp4HI	GCNGC	2 cut(s)	27, 518
FspBI	CTAG	2 cut(s)	633, 729
GlaI	GCGC	1 cut(s)	65
GluI	GCNGC	2 cut(s)	27, 518
GsaI	CCCAGC	1 cut(s)	513
HaeIII	GGCC	2 cut(s)	215, 661
HapII	CCGG	5 cut(s)	11, 23, 67, 477, 578
HhaI	GCGC	1 cut(s)	66
Hin1II	CATG	2 cut(s)	617, 632
Hin6I	GCGC	1 cut(s)	64
HinP1I	GCGC	1 cut(s)	64
HindIII	AAGCTT	1 cut(s)	564
HinfI	GANTC	4 cut(s)	111, 305, 319, 653
HpaII	CCGG	5 cut(s)	11, 23, 67, 477, 578
Hpy166II	GTNNAC	1 cut(s)	8
Hpy188I	TCNGA	4 cut(s)	264, 304, 369, 642
Hpy188III	TCNNGA	3 cut(s)	323, 325, 503
Hpy8I	GTNNAC	1 cut(s)	8
HpyAV	CCTTC	2 cut(s)	274, 556
HpyCH4III	ACNGT	3 cut(s)	206, 243, 411
HpyCH4V	TGCA	5 cut(s)	290, 382, 431, 486, 517
HpyF10VI	GCNNNNNNNGC	1 cut(s)	53
HpyF3I	CTNAG	5 cut(s)	105, 123, 338, 358, 366
Hsp92II	CATG	2 cut(s)	617, 632
HspAI	GCGC	1 cut(s)	64
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Kzo9I	GATC	2 cut(s)	13, 556
Lsp1109I	GCAGC	2 cut(s)	38, 529
LweI	GCATC	5 cut(s)	65, 169, 299, 495, 571
MaeI	CTAG	2 cut(s)	633, 729
MalI	GATC	2 cut(s)	15, 558
MboI	GATC	2 cut(s)	13, 556
MboII	GAAGA	2 cut(s)	44, 340
MflI	RGATCY	1 cut(s)	13
MluCI	AATT	4 cut(s)	99, 377, 446, 481
MlyI	GAGTC	1 cut(s)	313
MmeI	TCCRAC	2 cut(s)	665, 691
Mph1103I	ATGCAT	1 cut(s)	292
MroNI	GCCGGC	1 cut(s)	22
MseI	TTAA	2 cut(s)	153, 245
MspA1I	CMGCKG	1 cut(s)	29
MspI	CCGG	5 cut(s)	11, 23, 67, 477, 578
MspR9I	CCNGG	2 cut(s)	68, 78
Mva1269I	GAATGC	2 cut(s)	275, 433
MvaI	CCWGG	1 cut(s)	78
MwoI	GCNNNNNNNGC	1 cut(s)	53
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	1 cut(s)	68
NdeII	GATC	2 cut(s)	13, 556
NgoMIV	GCCGGC	1 cut(s)	22
NlaIII	CATG	2 cut(s)	617, 632
NlaIV	GGNNCC	1 cut(s)	15
NmeAIII	GCCGAG	1 cut(s)	654
NsiI	ATGCAT	1 cut(s)	292
NspI	RCATGY	2 cut(s)	617, 632
PaeI	GCATGC	1 cut(s)	632
PaeR7I	CTCGAG	2 cut(s)	323, 438
PaqCI	CACCTGC	1 cut(s)	535
PceI	AGGCCT	1 cut(s)	215
PciI	ACATGT	1 cut(s)	613
PctI	GAATGC	2 cut(s)	275, 433
PdiI	GCCGGC	1 cut(s)	24
PfeI	GAWTC	3 cut(s)	111, 305, 653
PfoI	TCCNGGA	1 cut(s)	76
PkrI	GCNGC	2 cut(s)	28, 519
PleI	GAGTC	1 cut(s)	313
PpsI	GAGTC	1 cut(s)	313
PscI	ACATGT	1 cut(s)	613
Psp6I	CCWGG	1 cut(s)	76
PspFI	CCCAGC	1 cut(s)	509
PspGI	CCWGG	1 cut(s)	76
PspN4I	GGNNCC	1 cut(s)	15
PspXI	VCTCGAGB	1 cut(s)	438
PsuI	RGATCY	1 cut(s)	13
PvuII	CAGCTG	1 cut(s)	29
SaqAI	TTAA	2 cut(s)	153, 245
SatI	GCNGC	2 cut(s)	27, 518
Sau3AI	GATC	2 cut(s)	13, 556
SchI	GAGTC	1 cut(s)	313
ScrFI	CCNGG	2 cut(s)	68, 78
SfaNI	GCATC	5 cut(s)	65, 169, 299, 495, 571
Sfr274I	CTCGAG	2 cut(s)	323, 438
SlaI	CTCGAG	2 cut(s)	323, 438
SmlI	CTYRAG	3 cut(s)	323, 438, 501
SmoI	CTYRAG	3 cut(s)	323, 438, 501
SphI	GCATGC	1 cut(s)	632
Sse9I	AATT	4 cut(s)	99, 377, 446, 481
SseBI	AGGCCT	1 cut(s)	215
SsiI	CCGC	3 cut(s)	115, 626, 690
SspMI	CTAG	2 cut(s)	633, 729
StuI	AGGCCT	1 cut(s)	215
StyD4I	CCNGG	2 cut(s)	66, 76
TaaI	ACNGT	3 cut(s)	206, 243, 411
TaqI	TCGA	5 cut(s)	238, 308, 324, 439, 706
TasI	AATT	4 cut(s)	99, 377, 446, 481
TfiI	GAWTC	3 cut(s)	111, 305, 653
Tru1I	TTAA	2 cut(s)	153, 245
Tru9I	TTAA	2 cut(s)	153, 245
TscAI	CASTG	3 cut(s)	66, 211, 579
TseI	GCWGC	2 cut(s)	26, 517
TspGWI	ACGGA	2 cut(s)	65, 151
TspRI	CASTG	3 cut(s)	66, 211, 579
XapI	RAATTY	1 cut(s)	99
XceI	RCATGY	2 cut(s)	617, 632
XhoI	CTCGAG	2 cut(s)	323, 438
XspI	CTAG	2 cut(s)	633, 729
Zsp2I	ATGCAT	1 cut(s)	292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.