FvH4_5g16340

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007684
Physical Location & Seq
Reverse (-)
9301053 .. 9304956
3904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16340.t3

Sequence Viewer

Length: 1248 bp
ATGGGGAGCTCAGATGAGAAAGTTGTGGCTGTGATCATGGTGGGTGGGCCCACTAAAGGCACTAGATTCCGGCCACTGTCATTGAATATTCCGAAGCCACTTTTTCCTTTAGCAGGACAACCAATGGTTCATCATCCGATTTCCGCTTGTAAAAAGATTTCTAACCTGGCACAAATCTTTCTCATTGGTTTCTATGAGGAGCGTGAGTTAACATTATATGTATCCTCGATCTCTAATGAGCTTAAGGTCCCTGTTAGATATTTGAGGGAAGACAGGCCACATGGTTCAGCTGGTGGACTTTATAACTTCAGAGATCTAATCATGGAAGACAGCCCGTCGCACATTTTCTTGCTGAACTGTGATGTTTGCTGCAGTTTTCCGCTTCCAGAAATGCTTGAGGCTCACAGAAAATATGGTGGGATGGGTACGATCCTGGTCATCAAGGTTTCTCCTGAGTCAGCAAGTCAGTTTGGGGAACTTGTAGCTGATCCGGTCACCAATGAACTGCTGCATTACACAGAGAAGCCTGAAACTTTTGTCAGTGACCGGATCAATTGTGGTGTCTACATATTCACACCAGATATTTTTAATGCCATAGAGGGTGTTTCTTCTCAGAAGAAGGACAGAGCTGACTTAAGACGTCTATCCAGTTTTGAAGCCCTGCACTCCGCAACAAGGAGTGCATCCACACATTTTGTGAGGTTAGATCAAGATATCCTATCCCCTCTTGCAGGAAAGAAGCAGTTATATACATATGAAACCATGGACTTCTGGGAACAGATTAAAACTCCTGGAATGTCCTTGAAATGTTCTGCTTTGTATCTTTCACAATTCCGAGTCACCAGCCCCCATCTTTTGGCAAGTGGAGATGGTTCAATGAGTGCCAGTATTTCTGGCGATGTTTACATTCATCCATCAGCAAAAGTACACCCCAGTGCTAAAATTGGCCCTAATGTCTCAATATCTGCAAATGCTCGTATAGGAGCTGGTGTGAGGCTCATAAGTTGTATCATACTCGACAATGTTGAAATTAAGGAAAATGCTGTTGTTATTCATGCAATTGTTGGATGGAAATCTTCCATCGGGAAATGGTCTCGTGTCCAGGCTAGTGGGGATTACAATTCAAAGCTTGGGATCACAATCCTTGGTGAAGACGTGACAGTTGAAGATGAAGTAGTGATTATCAACAGCATTGTCCTCCCCCATAAGACACTCAATCTCAGTGTTCAAGAGGAAATAATTCTTTGA

Protein Analysis

416

Amino Acids

45.58

Weight (kDa)

6.36

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 10 - 222 1.2e-27 Nucleotidyl transferase
LbH_EIF2B PF25084 303 - 378 4.2e-08 EIF2B subunit epsilon LbH domain
Hexapep PF00132 305 - 338 1.5e-06 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 307 - 424 4.4e-38 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 643
AccB7I CCANNNNNTGG 1 cut(s) 856
AccI GTMKAC 1 cut(s) 564
AciI CCGC 3 cut(s) 144, 380, 669
AclWI GGATC 4 cut(s) 424, 482, 557, 1142
AcoI YGGCCR 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 640
AfaI GTAC 2 cut(s) 427, 927
AfiI CCNNNNNNNGG 5 cut(s) 56, 113, 675, 731, 856
AflII CTTAAG 2 cut(s) 242, 634
AgsI TTSAA 8 cut(s) 85, 656, 805, 876, 1028, 1125, 1166, 1229
AhdI GACNNNNNGTC 1 cut(s) 334
AjiI CACGTC 1 cut(s) 1156
AjnI CCWGG 4 cut(s) 165, 432, 790, 1101
AleI CACNNNNGTG 1 cut(s) 933
AluBI AGCT 7 cut(s) 9, 241, 290, 485, 629, 986, 1129
AluI AGCT 7 cut(s) 9, 241, 290, 485, 629, 986, 1129
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 2 cut(s) 961, 1098
AlwI GGATC 4 cut(s) 424, 482, 557, 1142
AoxI GGCC 4 cut(s) 47, 71, 275, 946
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 369, 508
Asp700I GAANNNNTTC 1 cut(s) 1239
AspS9I GGNCC 4 cut(s) 47, 48, 247, 947
AsuHPI GGTGA 3 cut(s) 487, 832, 1160
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 51
BanII GRGCYC 2 cut(s) 11, 51
BauI CACGAG 1 cut(s) 1095
BbsI GAAGAC 3 cut(s) 276, 333, 1158
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 356, 495
BccI CCATC 6 cut(s) 415, 858, 863, 922, 1062, 1088
BciT130I CCWGG 4 cut(s) 167, 434, 792, 1103
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 961, 1098
BfaI CTAG 2 cut(s) 63, 1107
BfmI CTRYAG 1 cut(s) 370
BfrI CTTAAG 2 cut(s) 242, 634
BfuI GTATCC 1 cut(s) 232
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 370, 509
BlsI GCNGC 2 cut(s) 371, 510
Bme1390I CCNGG 4 cut(s) 167, 434, 792, 1103
Bme18I GGWCC 1 cut(s) 247
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 1156
BmgT120I GGNCC 4 cut(s) 47, 48, 247, 947
BmiI GGNNCC 2 cut(s) 49, 249
BmrFI CCNGG 4 cut(s) 167, 434, 792, 1103
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 1 cut(s) 692
BmuI ACTGGG 1 cut(s) 927
BpiI GAAGAC 3 cut(s) 276, 333, 1158
BpuEI CTTGAG 1 cut(s) 416
BsaBI GATNNNNATC 2 cut(s) 1072, 1139
BsaHI GRCGYC 1 cut(s) 640
BsaI GGTCTC 1 cut(s) 1098
BsaJI CCNNGG 2 cut(s) 762, 1144
BsaWI WCCGGW 2 cut(s) 490, 546
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 975, 1005
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 113, 675, 731, 856
Bse1I ACTGG 3 cut(s) 648, 885, 933
Bse8I GATNNNNATC 2 cut(s) 1072, 1139
BseBI CCWGG 4 cut(s) 167, 434, 792, 1103
BseDI CCNNGG 2 cut(s) 762, 1144
BseGI GGATG 5 cut(s) 133, 426, 683, 910, 1073
BseJI GATNNNNATC 2 cut(s) 1072, 1139
BseLI CCNNNNNNNGG 5 cut(s) 56, 113, 675, 731, 856
BseMII CTCAG 4 cut(s) 24, 444, 626, 1234
BseNI ACTGG 3 cut(s) 648, 885, 933
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 356, 495
BsgI GTGCAG 1 cut(s) 647
BshFI GGCC 4 cut(s) 49, 73, 277, 948
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 3 cut(s) 70, 491, 547
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 5 cut(s) 56, 113, 675, 731, 856
BsmAI GTCTC 2 cut(s) 961, 1098
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 4 cut(s) 49, 73, 277, 948
Bso31I GGTCTC 1 cut(s) 1098
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 11, 51
Bsp143I GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 3 cut(s) 144, 380, 669
BspANI GGCC 4 cut(s) 49, 73, 277, 948
BspCNI CTCAG 4 cut(s) 23, 445, 625, 1233
BspLI GGNNCC 2 cut(s) 49, 249
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 4 cut(s) 424, 482, 557, 1142
BspTI CTTAAG 2 cut(s) 242, 634
BspTNI GGTCTC 1 cut(s) 1098
BsrI ACTGG 3 cut(s) 648, 885, 933
BssECI CCNNGG 2 cut(s) 762, 1144
BssMI GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
BssNI GRCGYC 1 cut(s) 640
BssSI CACGAG 1 cut(s) 1095
BssT1I CCWWGG 2 cut(s) 762, 1144
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 4 cut(s) 167, 434, 792, 1103
Bst4CI ACNGT 3 cut(s) 78, 359, 1162
BstACI GRCGYC 1 cut(s) 640
BstAFI CTTAAG 2 cut(s) 242, 634
BstDEI CTNAG 4 cut(s) 10, 453, 612, 1220
BstDSI CCRYGG 1 cut(s) 762
BstEII GGTNACC 1 cut(s) 493
BstENI CCTNNNNNAGG 2 cut(s) 111, 729
BstF5I GGATG 5 cut(s) 133, 426, 683, 910, 1073
BstKTI GATC 8 cut(s) 36, 231, 316, 432, 490, 552, 709, 1137
BstMAI GTCTC 2 cut(s) 961, 1098
BstMBI GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
BstNI CCWGG 4 cut(s) 167, 434, 792, 1103
BstPI GGTNACC 1 cut(s) 493
BstSCI CCNGG 4 cut(s) 165, 432, 790, 1101
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 356, 495
BstV2I GAAGAC 3 cut(s) 276, 333, 1158
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 1109
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 4 cut(s) 49, 73, 277, 948
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 912
BtrI CACGTC 1 cut(s) 1156
BtsCI GGATG 5 cut(s) 133, 426, 683, 910, 1073
BtsIMutI CAGTG 4 cut(s) 74, 547, 940, 1228
Cfr13I GGNCC 4 cut(s) 47, 48, 247, 947
Csp6I GTAC 2 cut(s) 426, 926
CspCI CAANNNNNGTGG 4 cut(s) 63, 98, 676, 711
CviAII CATG 5 cut(s) 37, 281, 322, 763, 1055
CviQI GTAC 2 cut(s) 426, 926
DdeI CTNAG 4 cut(s) 10, 453, 612, 1220
DpnI GATC 8 cut(s) 35, 230, 315, 431, 489, 551, 708, 1136
DpnII GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 71
Eam1105I GACNNNNNGTC 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 762, 1144
Eco24I GRGCYC 2 cut(s) 11, 51
Eco31I GGTCTC 1 cut(s) 1098
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 292
Eco91I GGTNACC 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 2 cut(s) 111, 729
EcoO109I RGGNCCY 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 493
EcoRII CCWGG 4 cut(s) 165, 432, 790, 1101
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 2 cut(s) 762, 1144
EcoT38I GRGCYC 2 cut(s) 11, 51
ErhI CCWWGG 2 cut(s) 762, 1144
FaeI CATG 5 cut(s) 40, 284, 325, 766, 1058
FaqI GGGAC 1 cut(s) 233
FatI CATG 5 cut(s) 36, 280, 321, 762, 1054
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 2 cut(s) 370, 509
FokI GGATG 5 cut(s) 120, 433, 670, 897, 1080
FriOI GRGCYC 2 cut(s) 11, 51
Fsp4HI GCNGC 2 cut(s) 370, 509
FspBI CTAG 2 cut(s) 63, 1107
GluI GCNGC 2 cut(s) 370, 509
HaeIII GGCC 4 cut(s) 49, 73, 277, 948
HapII CCGG 3 cut(s) 70, 491, 547
Hin1I GRCGYC 1 cut(s) 640
Hin1II CATG 5 cut(s) 40, 284, 325, 766, 1058
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 3 cut(s) 66, 455, 837
HpaI GTTAAC 1 cut(s) 210
HpaII CCGG 3 cut(s) 70, 491, 547
HphI GGTGA 3 cut(s) 487, 832, 1160
Hpy166II GTNNAC 5 cut(s) 210, 296, 565, 904, 928
Hpy188I TCNGA 6 cut(s) 13, 93, 138, 311, 615, 836
Hpy188III TCNNGA 5 cut(s) 386, 452, 710, 1084, 1229
Hpy8I GTNNAC 5 cut(s) 210, 296, 565, 904, 928
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 613
HpyCH4III ACNGT 3 cut(s) 78, 359, 1162
HpyCH4IV ACGT 2 cut(s) 640, 1155
HpyCH4V TGCA 7 cut(s) 372, 511, 664, 683, 731, 968, 1058
HpyF3I CTNAG 4 cut(s) 10, 453, 612, 1220
HpySE526I ACGT 2 cut(s) 640, 1155
Hsp92I GRCGYC 1 cut(s) 640
Hsp92II CATG 5 cut(s) 40, 284, 325, 766, 1058
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 210
Kzo9I GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
LmnI GCTCC 3 cut(s) 6, 199, 983
Lsp1109I GCAGC 2 cut(s) 356, 495
LweI GCATC 1 cut(s) 692
MaeI CTAG 2 cut(s) 63, 1107
MaeII ACGT 2 cut(s) 640, 1155
MaeIII GTNAC 4 cut(s) 493, 542, 838, 1156
MalI GATC 8 cut(s) 35, 230, 315, 431, 489, 551, 708, 1136
MboI GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
MboII GAAGA 7 cut(s) 281, 338, 600, 628, 1068, 1163, 1178
MfeI CAATTG 2 cut(s) 553, 1059
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 11, 51
MluCI AATT 7 cut(s) 553, 830, 942, 1029, 1059, 1120, 1239
MlyI GAGTC 2 cut(s) 464, 846
MmeI TCCRAC 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 1239
MseI TTAA 6 cut(s) 209, 243, 588, 635, 783, 1032
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 290
MspCI CTTAAG 2 cut(s) 242, 634
MspI CCGG 3 cut(s) 70, 491, 547
MspR9I CCNGG 4 cut(s) 167, 434, 792, 1103
MunI CAATTG 2 cut(s) 553, 1059
MvaI CCWGG 4 cut(s) 167, 434, 792, 1103
NcoI CCATGG 1 cut(s) 762
NdeI CATATG 1 cut(s) 754
NdeII GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
NlaIII CATG 5 cut(s) 40, 284, 325, 766, 1058
NlaIV GGNNCC 2 cut(s) 49, 249
NmuCI GTSAC 4 cut(s) 493, 542, 838, 1156
OliI CACNNNNGTG 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 1239
PfeI GAWTC 1 cut(s) 66
PflMI CCANNNNNTGG 1 cut(s) 856
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 2 cut(s) 371, 510
PleI GAGTC 2 cut(s) 463, 845
PpsI GAGTC 2 cut(s) 463, 845
PpuMI RGGWCCY 1 cut(s) 247
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 4 cut(s) 165, 432, 790, 1101
PspEI GGTNACC 1 cut(s) 493
PspGI CCWGG 4 cut(s) 165, 432, 790, 1101
PspN4I GGNNCC 2 cut(s) 49, 249
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 4 cut(s) 47, 48, 247, 947
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 427, 927
RsaNI GTAC 2 cut(s) 426, 926
RseI CAYNNNNRTG 1 cut(s) 933
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 6 cut(s) 209, 243, 588, 635, 783, 1032
SatI GCNGC 2 cut(s) 370, 509
Sau3AI GATC 8 cut(s) 33, 228, 313, 429, 487, 549, 706, 1134
Sau96I GGNCC 4 cut(s) 47, 48, 247, 947
SchI GAGTC 2 cut(s) 464, 846
ScrFI CCNGG 4 cut(s) 167, 434, 792, 1103
SduI GDGCHC 2 cut(s) 11, 51
SfaNI GCATC 1 cut(s) 692
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 3 cut(s) 242, 395, 634
SmoI CTYRAG 3 cut(s) 242, 395, 634
Sse9I AATT 7 cut(s) 553, 830, 942, 1029, 1059, 1120, 1239
SsiI CCGC 3 cut(s) 144, 380, 669
SspI AATATT 1 cut(s) 88
SspMI CTAG 2 cut(s) 63, 1107
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 165, 432, 790, 1101
StyI CCWWGG 2 cut(s) 762, 1144
TaaI ACNGT 3 cut(s) 78, 359, 1162
TaiI ACGT 2 cut(s) 643, 1158
TaqI TCGA 2 cut(s) 227, 1017
TasI AATT 7 cut(s) 553, 830, 942, 1029, 1059, 1120, 1239
TatI WGTACW 1 cut(s) 925
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 6 cut(s) 209, 243, 588, 635, 783, 1032
Tru9I TTAA 6 cut(s) 209, 243, 588, 635, 783, 1032
TscAI CASTG 4 cut(s) 81, 547, 940, 1228
TseFI GTSAC 4 cut(s) 493, 542, 838, 1156
TseI GCWGC 2 cut(s) 369, 508
Tsp45I GTSAC 4 cut(s) 493, 542, 838, 1156
TspDTI ATGAA 6 cut(s) 119, 516, 771, 899, 1043, 1185
TspRI CASTG 4 cut(s) 81, 547, 940, 1228
Van91I CCANNNNNTGG 1 cut(s) 856
Vha464I CTTAAG 2 cut(s) 242, 634
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 2 cut(s) 111, 729
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 2 cut(s) 63, 1107
ZraI GACGTC 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.