pycom06g16060

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007684
Physical Location & Seq
Forward (+)
20509264 .. 20510270
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g16060.1

Sequence Viewer

Length: 384 bp
ATGGGAACAATTCTTGTGATCAAGGTTTCCGCTGAATCAGCCAGTCAGTTTGGGGAACTGGTAGCTGATCCGTTCACTAATGAACTGTTGCATTACACCGAGAAGCCTGAGACATTTGTCAGCGACAGGATAAATTGCGGTGTCTACATATTTACACCAGACATTTTTAACGCCGTTGAAGGTGTTTCCACTCAGTGGAAGGACAGAGCTGATTTAAGACGACTCTCCAGCTTCGAATCCCTTCATTCAGCAACAAGGAACGCTCCCACACATTTTGTAAGGCTGGATCAAGATATCCTATCACCTCTTGCAGGGAAGAAGCAGTTATACACATATGAAACCTTGGACTTCTTGGATCGATCAAAACTCCTGGAATGTCGCTAA

Protein Analysis

128

Amino Acids

14.45

Weight (kDa)

5.32

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 3 - 60 3.6e-06 Nucleotidyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 195
AccI GTMKAC 1 cut(s) 144
AciI CCGC 2 cut(s) 30, 138
AclWI GGATC 3 cut(s) 62, 294, 363
AdeI CACNNNGTG 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 195, 311
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 3 cut(s) 65, 209, 231
AluI AGCT 3 cut(s) 65, 209, 231
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 3 cut(s) 62, 294, 363
Asp700I GAANNNNTTC 1 cut(s) 240
AsuHPI GGTGA 1 cut(s) 294
AsuII TTCGAA 1 cut(s) 234
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 371
BoxI GACNNNNGTC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 211
Bpu14I TTCGAA 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 311
Bse1I ACTGG 2 cut(s) 42, 63
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 195, 311
BseMII CTCAG 2 cut(s) 99, 206
BseNI ACTGG 2 cut(s) 42, 63
BshVI ATCGAT 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 195, 311
BsmAI GTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 234
Bsp143I GATC 5 cut(s) 18, 67, 286, 355, 359
BspACI CCGC 2 cut(s) 30, 138
BspCNI CTCAG 2 cut(s) 100, 205
BspDI ATCGAT 1 cut(s) 358
BspPI GGATC 3 cut(s) 62, 294, 363
BspT104I TTCGAA 1 cut(s) 234
BsrI ACTGG 2 cut(s) 42, 63
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 5 cut(s) 18, 67, 286, 355, 359
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 108, 192
BstENI CCTNNNNNAGG 1 cut(s) 309
BstKTI GATC 5 cut(s) 21, 70, 289, 358, 362
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 5 cut(s) 18, 67, 286, 355, 359
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 1 cut(s) 371
BstPAI GACNNNNGTC 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 358
BsuTUI ATCGAT 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 200
ClaI ATCGAT 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviJI RGCY 6 cut(s) 41, 65, 106, 209, 231, 283
CviKI_1 RGCY 6 cut(s) 41, 65, 106, 209, 231, 283
DdeI CTNAG 2 cut(s) 108, 192
DpnI GATC 5 cut(s) 20, 69, 288, 357, 361
DpnII GATC 5 cut(s) 18, 67, 286, 355, 359
DraIII CACNNNGTG 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 342
Eco32I GATATC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaiI YATR 4 cut(s) 149, 328, 334, 336
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 1 cut(s) 18
FblI GTMKAC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 211
HinfI GANTC 3 cut(s) 35, 222, 236
HphI GGTGA 1 cut(s) 294
Hpy166II GTNNAC 2 cut(s) 75, 145
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 2 cut(s) 75, 145
HpyAV CCTTC 3 cut(s) 173, 193, 251
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 91, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 2 cut(s) 108, 192
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 5 cut(s) 18, 67, 286, 355, 359
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 9 cut(s) 44, 55, 112, 120, 171, 241, 269, 297, 356
MalI GATC 5 cut(s) 20, 69, 288, 357, 361
MboI GATC 5 cut(s) 18, 67, 286, 355, 359
MboII GAAGA 1 cut(s) 328
MluCI AATT 2 cut(s) 9, 133
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 2 cut(s) 168, 215
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeI CATATG 1 cut(s) 334
NdeII GATC 5 cut(s) 18, 67, 286, 355, 359
NspV TTCGAA 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 35, 236
PflMI CCANNNNNTGG 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 369
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
SaqAI TTAA 2 cut(s) 168, 215
Sau3AI GATC 5 cut(s) 18, 67, 286, 355, 359
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 1 cut(s) 371
SetI ASST 7 cut(s) 27, 67, 184, 211, 233, 307, 344
SfuI TTCGAA 1 cut(s) 234
Sse9I AATT 2 cut(s) 9, 133
SsiI CCGC 2 cut(s) 30, 138
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 234, 358
TasI AATT 2 cut(s) 9, 133
TfiI GAWTC 2 cut(s) 35, 236
Tru1I TTAA 2 cut(s) 168, 215
Tru9I TTAA 2 cut(s) 168, 215
TscAI CASTG 1 cut(s) 200
TspDTI ATGAA 3 cut(s) 96, 233, 351
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 1 cut(s) 200
Van91I CCANNNNNTGG 1 cut(s) 195
XagI CCTNNNNNAGG 1 cut(s) 309
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.