Rw7G002230

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007684
Physical Location & Seq
Forward (+)
1858503 .. 1861933
3431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002230.1

Sequence Viewer

Length: 1260 bp
ATGGGGAGCTCAGAGGAGAAAGTTGTTGCTGTGATCATGGTGGGTGGGCCCACTAAAGGCACTAGATTCCGGCCACTTTCATTGAATATTCCGAAGCCACTTTTTCCTTTAGCGGACAACCAATGGTTCATCATCCAATTTCTGCTTGTAAAAAGGTATATTCTTGAAATTCCTAACCTGGGGCAAATCTTTCTCATTGGTTTCTATGAGGAGCGTGAGTTATCATTATATGTATCCGCAATCTCTAATGAGCTTAAGGTCCCCGTTAGATATTTGAGGGAAGACAGGCCACATGGTTCAGCTGGTGGACTTTATAACTTCAGAGATCTGATCATGGAAGACAGCCCATCGCACATTTTCTTGCTGAACTGTGATGTTTGCTGCAGTTTTCCACTTCCAGAAATGCTTGAGGCTCACAGAAAATATGGTGGGATGGGAACAATCCTGGTAATCAAGGTTTCTGCTGAGTCAGCAAGTCAGTTTGGGGAACTTGTAGCTGATCCAGTCACCAATGAACTGCTGCATTACACAGAGAAGCCCGAGACTTTTGTCAGTGACCGGATAAATTGTGGTGTCTACATATTCACACCAGATATTTTTAATGCCATAGAGGATGTTTCCTCTCAGCGGAAGGACGGAGCTGACTTAAGACGTCTATCCAGTTTTGAAGCCCTGCACTCCGCAACAAGGAGTGCATCCACACATTTTGTAAGGTTGGATCAAGATATCCTATCCCCTCTTGCAGGAAAGAAGCAGTTATATACATATGAAACCATGGACTTCTGGGAACAGATTAAAACTCCTGGAATGTCCTTGAAATGTTCTGGGTTGTATCTTTCACAATTCAGAGTCACCAGTCCCCATCTGTTGGTGAGTGGGGATGGTACAAAGAGTGCCAGTATTTCTGGTGATGTTTACATCCATCCATCAGCAAAAGTACACCCAAGTGCTAAAATTGGCCCTAATGTCTCAATATCTGCAAATGCTCGTATAGGAGCTGGTGTGAGGCTCATAAGTTGTATCATACTTGACAATGTCGAAATTAAGGAAAATGCAGTTGTTATGCATGCAATTATTGGATGGAAATCTTCTATTGGGAAATGGTCTCGTATCCAGGCTAGTGGGAATTACAATTCTAAGCTCGGGATCACAATCCTTGGTGAAGATGTGACAGTTGAAGATGAAGTCGTGGTTATCAACAGCATTGTCCTCCCCCATAAGACGCTCAATCTGAGTGTTCAGGAGGAAATAATTCTGTGA

Protein Analysis

419

Amino Acids

46.39

Weight (kDa)

6.03

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 10 - 209 1.8e-27 Nucleotidyl transferase
LbH_EIF2B PF25084 297 - 373 9.8e-09 EIF2B subunit epsilon LbH domain
Hexapep PF00132 299 - 332 1.7e-06 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 301 - 418 6.7e-39 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 315
AatII GACGTC 1 cut(s) 655
AccB7I CCANNNNNTGG 1 cut(s) 868
AccI GTMKAC 1 cut(s) 576
AciI CCGC 4 cut(s) 113, 237, 628, 681
AclWI GGATC 3 cut(s) 494, 726, 1154
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 304
AcyI GRCGYC 1 cut(s) 652
AfaI GTAC 2 cut(s) 886, 939
AfiI CCNNNNNNNGG 6 cut(s) 56, 179, 627, 687, 743, 868
AflII CTTAAG 2 cut(s) 254, 646
AgsI TTSAA 5 cut(s) 85, 167, 668, 817, 1178
AjnI CCWGG 4 cut(s) 177, 444, 802, 1113
AleI CACNNNNGTG 1 cut(s) 945
AluBI AGCT 7 cut(s) 9, 253, 302, 497, 641, 998, 1141
AluI AGCT 7 cut(s) 9, 253, 302, 497, 641, 998, 1141
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 536, 973, 1110
AlwI GGATC 3 cut(s) 494, 726, 1154
Ama87I CYCGRG 2 cut(s) 539, 1142
AoxI GGCC 4 cut(s) 47, 71, 287, 958
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 381, 520
ApoI RAATTY 1 cut(s) 168
Asp700I GAANNNNTTC 1 cut(s) 1251
AspS9I GGNCC 4 cut(s) 47, 48, 259, 959
AsuHPI GGTGA 5 cut(s) 499, 844, 883, 920, 1172
AvaI CYCGRG 2 cut(s) 539, 1142
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 51
BanII GRGCYC 2 cut(s) 11, 51
BbsI GAAGAC 2 cut(s) 288, 345
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 368, 507
BccI CCATC 7 cut(s) 355, 427, 870, 875, 930, 934, 1074
BciT130I CCWGG 4 cut(s) 179, 446, 804, 1115
BciVI GTATCC 2 cut(s) 244, 1121
BclI TGATCA 2 cut(s) 33, 330
BcoDI GTCTC 3 cut(s) 536, 973, 1110
BfaI CTAG 2 cut(s) 63, 1119
BfmI CTRYAG 1 cut(s) 382
BfrI CTTAAG 2 cut(s) 254, 646
BfuI GTATCC 2 cut(s) 244, 1121
BglII AGATCT 1 cut(s) 325
BisI GCNGC 2 cut(s) 382, 521
BlsI GCNGC 2 cut(s) 383, 522
Bme1390I CCNGG 4 cut(s) 179, 446, 804, 1115
Bme18I GGWCC 1 cut(s) 259
BmeT110I CYCGRG 2 cut(s) 539, 1142
BmgT120I GGNCC 4 cut(s) 47, 48, 259, 959
BmiI GGNNCC 2 cut(s) 49, 261
BmrFI CCNGG 4 cut(s) 179, 446, 804, 1115
BmsI GCATC 1 cut(s) 704
BoxI GACNNNNGTC 1 cut(s) 548
BpiI GAAGAC 2 cut(s) 288, 345
BpuEI CTTGAG 1 cut(s) 428
BsaBI GATNNNNATC 2 cut(s) 1084, 1151
BsaHI GRCGYC 1 cut(s) 652
BsaI GGTCTC 1 cut(s) 1110
BsaJI CCNNGG 3 cut(s) 178, 774, 1156
BsaWI WCCGGW 1 cut(s) 558
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 987, 1017
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 179, 627, 687, 743, 868
Bse1I ACTGG 4 cut(s) 503, 660, 855, 897
Bse8I GATNNNNATC 2 cut(s) 1084, 1151
BseBI CCWGG 4 cut(s) 179, 446, 804, 1115
BseDI CCNNGG 3 cut(s) 178, 774, 1156
BseGI GGATG 8 cut(s) 132, 438, 619, 695, 886, 918, 922, 1085
BseJI GATNNNNATC 2 cut(s) 1084, 1151
BseLI CCNNNNNNNGG 6 cut(s) 56, 179, 627, 687, 743, 868
BseMII CTCAG 4 cut(s) 24, 456, 638, 1223
BseNI ACTGG 4 cut(s) 503, 660, 855, 897
BseRI GAGGAG 2 cut(s) 29, 224
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 368, 507
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 4 cut(s) 49, 73, 289, 960
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 2 cut(s) 539, 1142
BsiSI CCGG 2 cut(s) 70, 559
BslFI GGGAC 2 cut(s) 245, 843
BslI CCNNNNNNNGG 6 cut(s) 56, 179, 627, 687, 743, 868
BsmAI GTCTC 3 cut(s) 536, 973, 1110
BsmFI GGGAC 2 cut(s) 245, 843
BsnI GGCC 4 cut(s) 49, 73, 289, 960
Bso31I GGTCTC 1 cut(s) 1110
BsoBI CYCGRG 2 cut(s) 539, 1142
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 11, 51
Bsp143I GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 4 cut(s) 113, 237, 628, 681
BspANI GGCC 4 cut(s) 49, 73, 289, 960
BspCNI CTCAG 4 cut(s) 23, 457, 637, 1224
BspLI GGNNCC 2 cut(s) 49, 261
BspMAI CTGCAG 1 cut(s) 386
BspPI GGATC 3 cut(s) 494, 726, 1154
BspTI CTTAAG 2 cut(s) 254, 646
BspTNI GGTCTC 1 cut(s) 1110
BsrI ACTGG 4 cut(s) 503, 660, 855, 897
BssECI CCNNGG 3 cut(s) 178, 774, 1156
BssMI GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
BssNI GRCGYC 1 cut(s) 652
BssT1I CCWWGG 2 cut(s) 774, 1156
Bst2UI CCWGG 4 cut(s) 179, 446, 804, 1115
Bst4CI ACNGT 2 cut(s) 371, 1174
BstACI GRCGYC 1 cut(s) 652
BstAFI CTTAAG 2 cut(s) 254, 646
BstC8I GCNNGC 1 cut(s) 1068
BstDEI CTNAG 5 cut(s) 10, 465, 624, 1137, 1232
BstDSI CCRYGG 1 cut(s) 774
BstENI CCTNNNNNAGG 1 cut(s) 741
BstF5I GGATG 8 cut(s) 132, 438, 619, 695, 886, 918, 922, 1085
BstKTI GATC 6 cut(s) 36, 328, 333, 502, 721, 1149
BstMAI GTCTC 3 cut(s) 536, 973, 1110
BstMBI GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 4 cut(s) 179, 446, 804, 1115
BstNSI RCATGY 1 cut(s) 1070
BstPAI GACNNNNGTC 1 cut(s) 548
BstSCI CCNGG 4 cut(s) 177, 444, 802, 1113
BstSFI CTRYAG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 368, 507
BstV2I GAAGAC 2 cut(s) 288, 345
BstX2I RGATCY 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 325
BsuI GTATCC 2 cut(s) 244, 1121
BsuRI GGCC 4 cut(s) 49, 73, 289, 960
BtgI CCRYGG 1 cut(s) 774
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 8 cut(s) 132, 438, 619, 695, 886, 918, 922, 1085
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 1068
Cfr13I GGNCC 4 cut(s) 47, 48, 259, 959
CseI GACGC 1 cut(s) 1231
Csp6I GTAC 2 cut(s) 885, 938
CspCI CAANNNNNGTGG 4 cut(s) 63, 98, 688, 723
CviAII CATG 5 cut(s) 37, 293, 334, 775, 1067
CviQI GTAC 2 cut(s) 885, 938
DdeI CTNAG 5 cut(s) 10, 465, 624, 1137, 1232
DpnI GATC 6 cut(s) 35, 327, 332, 501, 720, 1148
DpnII GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
EaeI YGGCCR 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 774, 1156
Eco24I GRGCYC 2 cut(s) 11, 51
Eco31I GGTCTC 1 cut(s) 1110
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 304
Eco88I CYCGRG 2 cut(s) 539, 1142
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 4 cut(s) 177, 444, 802, 1113
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 774, 1156
EcoT22I ATGCAT 1 cut(s) 1068
EcoT38I GRGCYC 2 cut(s) 11, 51
ErhI CCWWGG 2 cut(s) 774, 1156
FaeI CATG 5 cut(s) 40, 296, 337, 778, 1070
FaqI GGGAC 2 cut(s) 245, 843
FatI CATG 5 cut(s) 36, 292, 333, 774, 1066
FauNDI CATATG 1 cut(s) 766
FbaI TGATCA 2 cut(s) 33, 330
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 382, 521
FokI GGATG 8 cut(s) 119, 445, 626, 682, 893, 905, 909, 1092
FriOI GRGCYC 2 cut(s) 11, 51
Fsp4HI GCNGC 2 cut(s) 382, 521
FspBI CTAG 2 cut(s) 63, 1119
GluI GCNGC 2 cut(s) 382, 521
HaeIII GGCC 4 cut(s) 49, 73, 289, 960
HapII CCGG 2 cut(s) 70, 559
HgaI GACGC 1 cut(s) 1231
Hin1I GRCGYC 1 cut(s) 652
Hin1II CATG 5 cut(s) 40, 296, 337, 778, 1070
HinfI GANTC 3 cut(s) 66, 467, 849
HpaII CCGG 2 cut(s) 70, 559
HphI GGTGA 5 cut(s) 499, 844, 883, 920, 1172
Hpy166II GTNNAC 4 cut(s) 308, 577, 916, 940
Hpy188I TCNGA 6 cut(s) 13, 93, 323, 330, 848, 1233
Hpy188III TCNNGA 5 cut(s) 164, 398, 722, 1144, 1241
Hpy8I GTNNAC 4 cut(s) 308, 577, 916, 940
HpyAV CCTTC 1 cut(s) 625
HpyCH4III ACNGT 2 cut(s) 371, 1174
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 9 cut(s) 384, 523, 676, 695, 743, 980, 1055, 1066, 1070
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 5 cut(s) 10, 465, 624, 1137, 1232
HpySE526I ACGT 1 cut(s) 652
Hsp92I GRCGYC 1 cut(s) 652
Hsp92II CATG 5 cut(s) 40, 296, 337, 778, 1070
Ksp22I TGATCA 2 cut(s) 33, 330
Kzo9I GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
LmnI GCTCC 4 cut(s) 6, 211, 638, 995
Lsp1109I GCAGC 2 cut(s) 368, 507
LweI GCATC 1 cut(s) 704
MaeI CTAG 2 cut(s) 63, 1119
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 4 cut(s) 505, 554, 850, 1168
MalI GATC 6 cut(s) 35, 327, 332, 501, 720, 1148
MboI GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
MboII GAAGA 5 cut(s) 293, 350, 1080, 1175, 1190
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 11, 51
MlyI GAGTC 2 cut(s) 476, 858
MmeI TCCRAC 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 1068
MroXI GAANNNNTTC 1 cut(s) 1251
MseI TTAA 5 cut(s) 255, 600, 647, 795, 1044
MslI CAYNNNNRTG 1 cut(s) 945
MspA1I CMGCKG 2 cut(s) 302, 628
MspCI CTTAAG 2 cut(s) 254, 646
MspI CCGG 2 cut(s) 70, 559
MspR9I CCNGG 4 cut(s) 179, 446, 804, 1115
MvaI CCWGG 4 cut(s) 179, 446, 804, 1115
MwoI GCNNNNNNNGC 1 cut(s) 470
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 766
NdeII GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
NlaIII CATG 5 cut(s) 40, 296, 337, 778, 1070
NlaIV GGNNCC 2 cut(s) 49, 261
NmuCI GTSAC 4 cut(s) 505, 554, 850, 1168
NsiI ATGCAT 1 cut(s) 1068
NspI RCATGY 1 cut(s) 1070
OliI CACNNNNGTG 1 cut(s) 945
PaeI GCATGC 1 cut(s) 1070
PdmI GAANNNNTTC 1 cut(s) 1251
PfeI GAWTC 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 1034
PflMI CCANNNNNTGG 1 cut(s) 868
PfoI TCCNGGA 1 cut(s) 802
PkrI GCNGC 2 cut(s) 383, 522
PleI GAGTC 2 cut(s) 475, 857
PpsI GAGTC 2 cut(s) 475, 857
PpuMI RGGWCCY 1 cut(s) 259
PshAI GACNNNNGTC 1 cut(s) 548
PsiI TTATAA 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 4 cut(s) 177, 444, 802, 1113
PspGI CCWGG 4 cut(s) 177, 444, 802, 1113
PspN4I GGNNCC 2 cut(s) 49, 261
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 4 cut(s) 47, 48, 259, 959
PspPPI RGGWCCY 1 cut(s) 259
PstI CTGCAG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 325
PsyI GACNNNGTC 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 886, 939
RsaNI GTAC 2 cut(s) 885, 938
RseI CAYNNNNRTG 1 cut(s) 945
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 5 cut(s) 255, 600, 647, 795, 1044
SatI GCNGC 2 cut(s) 382, 521
Sau3AI GATC 6 cut(s) 33, 325, 330, 499, 718, 1146
Sau96I GGNCC 4 cut(s) 47, 48, 259, 959
SchI GAGTC 2 cut(s) 476, 858
ScrFI CCNGG 4 cut(s) 179, 446, 804, 1115
SduI GDGCHC 2 cut(s) 11, 51
SfaNI GCATC 1 cut(s) 704
SfcI CTRYAG 1 cut(s) 382
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 3 cut(s) 254, 407, 646
SmoI CTYRAG 3 cut(s) 254, 407, 646
SphI GCATGC 1 cut(s) 1070
SsiI CCGC 4 cut(s) 113, 237, 628, 681
SspI AATATT 1 cut(s) 88
SspMI CTAG 2 cut(s) 63, 1119
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 177, 444, 802, 1113
StyI CCWWGG 2 cut(s) 774, 1156
TaaI ACNGT 2 cut(s) 371, 1174
TaiI ACGT 1 cut(s) 655
TaqI TCGA 1 cut(s) 1038
TatI WGTACW 1 cut(s) 937
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 5 cut(s) 255, 600, 647, 795, 1044
Tru9I TTAA 5 cut(s) 255, 600, 647, 795, 1044
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 4 cut(s) 505, 554, 850, 1168
TseI GCWGC 2 cut(s) 381, 520
Tsp45I GTSAC 4 cut(s) 505, 554, 850, 1168
TspDTI ATGAA 5 cut(s) 69, 118, 528, 783, 1197
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 1 cut(s) 559
Tth111I GACNNNGTC 1 cut(s) 1034
Van91I CCANNNNNTGG 1 cut(s) 868
Vha464I CTTAAG 2 cut(s) 254, 646
VpaK11BI GGWCC 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 741
XapI RAATTY 1 cut(s) 168
XceI RCATGY 1 cut(s) 1070
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 1251
XspI CTAG 2 cut(s) 63, 1119
ZraI GACGTC 1 cut(s) 653
Zsp2I ATGCAT 1 cut(s) 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.