Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007684

Physical Location & Seq

Genomic Coordinates Forward (+)

27962858 .. 27966575

Gene Structure 3718 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1187500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGGGAGCTCGGACGACAAGGTGGTTGCTGTGATCATGGTGGGTGGGCCCACTAAAGGAACTAGATTCCGGCCATTGTCACTGAATATACCGAAGCCGCTTTTTCCATTAGCAGGACAACCAATGGTTCATCATCCGATTTCCGCTTGTAAAAGGATTCCGAACCTCACACAAATTTTCCTCATTGGTTTCTACGAGGAGCGTGAATTCGCGTTATATGTGTCCTCAATCTCTAATGAGCTTAAAGTCCCTGTTAGATATTTGAGGGAAGACATGCCACATGGATCAGCTGGTGGACTTTATAATTTCAGAGATCTGATCATGGAAGACAACCCGTCGCACATTGTCTTGTTGAATTGTGATGTTTGCTGCACTTTTCCGCTTCCAGAAATGCTTGAGGCTCACAAAAAATATGGTGCTATGGGAACAATCCTTGTGATCAAGGTTTCTGCTGAATCAGCCAGTCAGTTTGGGGAACTGGTAGCTGATCCGGTCACCAATGAACTGTTGCATTACACCGAGAAGCCTGAGACTTTTGTCAGCGACAGGATAAATTGTGGTGTCTACATATTTACACCAGACATTTTTAACACCATCAAAGGTGTTTCCACTCAGTGGAAGGACAGAGCTGATTTAAGACGTCTGTCCAGCTTCGAATCCCTTCATTCAGCAACAAGGAATGCTCCCACGCATTTTGTAAGGCTAGATCAAGATATCCTATCGCCTCTTGCGGGGAAAAAGCAGTTATACACATATGAAACCTTGGACTTCTGGGAACAGATCAAAACTCCTGGAATGTCATTAAGGTGTTCTGGTTTGTATCTTGCACAATTTCGATTCACCAATCCACATCTCTTGGTGAGTGGAGATGGTACAAAGAGTGCCAGTATTACCGGTGATGTTTATGTTCATCCATCAGCAAAAGTACATCCAAGTGCTAAGATTGGTCCCAATGTCTCAATATCTGCAAATGCTCGCATAGGAGCTGGTGTGAGGCTCATAAGTTGTATCATCCTTGACAATGTCGAAATAAAGGAAAACGCAGTTGTTATTCATGCAATTGTTGGATGGAAATCTTCAATCGGGAGATGGTCTCGTGTCCAGGCTACTGGGGATTACAATTCAAAGCTCGGGATCACGATCCTTGGTGAAGCAGTGACAGTTGAAGATGAAGTGGTGGTTATAAACAGCATTGTCCTGCCCCATAAGACTCTCAACGTCAGCGTTCAGGAAGAAATCATTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004475 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008905 GO:0009058 GO:0009225 GO:0009226 GO:0009298 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0019673 GO:0034641 GO:0034654 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0046483 GO:0055086 GO:0070568 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00520 ko01100 ko01110 map00051 map00520 map01100 map01110

Pfam Domains

Protein Families

Hexapep NTP_transferase

Protein Analysis

416

Amino Acids

45.79

Weight (kDa)

6.67

Isoelectric Point (pI)

34.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTP_transferase	PF00483	10 - 205	2.2e-32	Nucleotidyl transferase
LbH_EIF2B	PF25084	293 - 368	9.2e-08	EIF2B subunit epsilon LbH domain
GMPPB_C	PF25087	297 - 414	2.9e-37	GMPPB C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74910 AT1G74910 AT1G74910
fragaria_vesca	FvH4_5g16340 FvH4_5g16340 FvH4_5g16340 FvH4_5g16340 FvH4_5g16340
malus_domestica	MD06G1181600.v1.1 MD14G1187500.v1.1
prunus_persica	Prupe.5G179900_v2.0.a1 Prupe.5G179900_v2.0.a1 Prupe.5G179900_v2.0.a1
pyrus_communis	pycom06g16060
rosa_chinensis	RchiOBHm_Chr7g0179791
rosa_multiflora	Rmu_sc0001754.1_g000007
rosa_roxburghii	Rroxscaffold_3G00273470
rosa_rugosa	Rorug06G0428100 Rorug06G0428200
rosa_samantha	Rh7AG027800 Rh7BG027000 Rh7CG028900 Rh7DG028400
rosa_wichuraiana	Rw7G002230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	303, 1184
AatII	GACGTC	1 cut(s)	643
AccB7I	CCANNNNNTGG	1 cut(s)	615
AccI	GTMKAC	1 cut(s)	564
AccII	CGCG	1 cut(s)	212
AciI	CCGC	4 cut(s)	98, 144, 380, 731
AclWI	GGATC	4 cut(s)	292, 482, 1135, 1142
AcoI	YGGCCR	1 cut(s)	71
AcsI	RAATTY	2 cut(s)	174, 206
AcyI	GRCGYC	1 cut(s)	640
AdeI	CACNNNGTG	1 cut(s)	615
AfaI	GTAC	2 cut(s)	874, 927
AfiI	CCNNNNNNNGG	4 cut(s)	56, 113, 615, 731
AgeI	ACCGGT	1 cut(s)	893
AgsI	TTSAA	4 cut(s)	355, 1080, 1125, 1166
AhdI	GACNNNNNGTC	1 cut(s)	334
AjnI	CCWGG	2 cut(s)	790, 1101
AluBI	AGCT	8 cut(s)	9, 241, 290, 485, 629, 651, 986, 1129
AluI	AGCT	8 cut(s)	9, 241, 290, 485, 629, 651, 986, 1129
Alw21I	GWGCWC	1 cut(s)	11
Alw26I	GTCTC	3 cut(s)	524, 961, 1098
AlwI	GGATC	4 cut(s)	292, 482, 1135, 1142
Ama87I	CYCGRG	1 cut(s)	1130
AoxI	GGCC	2 cut(s)	47, 71
ApaI	GGGCCC	1 cut(s)	51
ApeKI	GCWGC	1 cut(s)	369
ApoI	RAATTY	2 cut(s)	174, 206
AsiGI	ACCGGT	1 cut(s)	893
Asp700I	GAANNNNTTC	2 cut(s)	660, 1239
AspS9I	GGNCC	3 cut(s)	47, 48, 947
AsuHPI	GGTGA	5 cut(s)	487, 832, 871, 908, 1160
AsuII	TTCGAA	1 cut(s)	654
AvaI	CYCGRG	1 cut(s)	1130
AvaII	GGWCC	1 cut(s)	947
BaeGI	GKGCMC	1 cut(s)	51
BanII	GRGCYC	2 cut(s)	11, 51
BauI	CACGAG	1 cut(s)	1095
BbsI	GAAGAC	2 cut(s)	276, 333
Bbv12I	GWGCWC	1 cut(s)	11
BbvI	GCAGC	1 cut(s)	356
BccI	CCATC	5 cut(s)	602, 863, 922, 1062, 1083
BciT130I	CCWGG	2 cut(s)	792, 1103
BclI	TGATCA	3 cut(s)	33, 318, 438
BcoDI	GTCTC	3 cut(s)	524, 961, 1098
BfaI	CTAG	2 cut(s)	63, 704
BglII	AGATCT	1 cut(s)	313
BisI	GCNGC	2 cut(s)	98, 370
BlsI	GCNGC	2 cut(s)	99, 371
Bme1390I	CCNGG	2 cut(s)	792, 1103
Bme18I	GGWCC	1 cut(s)	947
BmeRI	GACNNNNNGTC	1 cut(s)	334
BmeT110I	CYCGRG	1 cut(s)	1130
BmgT120I	GGNCC	3 cut(s)	47, 48, 947
BmiI	GGNNCC	2 cut(s)	49, 949
BmrFI	CCNGG	2 cut(s)	792, 1103
BmrI	ACTGGG	1 cut(s)	1119
BmuI	ACTGGG	1 cut(s)	1119
BoxI	GACNNNNGTC	2 cut(s)	536, 643
BpiI	GAAGAC	2 cut(s)	276, 333
BplI	GAGNNNNNCTC	2 cut(s)	1078, 1110
Bpu14I	TTCGAA	1 cut(s)	654
BpuEI	CTTGAG	1 cut(s)	416
BsaBI	GATNNNNATC	2 cut(s)	1072, 1139
BsaHI	GRCGYC	1 cut(s)	640
BsaI	GGTCTC	1 cut(s)	1098
BsaJI	CCNNGG	2 cut(s)	762, 1144
BsaWI	WCCGGW	2 cut(s)	490, 893
BsaXI	ACNNNNNCTCC	2 cut(s)	975, 1005
Bsc4I	CCNNNNNNNGG	4 cut(s)	56, 113, 615, 731
Bse118I	RCCGGY	1 cut(s)	893
Bse1I	ACTGG	4 cut(s)	462, 483, 885, 1114
Bse8I	GATNNNNATC	2 cut(s)	1072, 1139
BseBI	CCWGG	2 cut(s)	792, 1103
BseDI	CCNNGG	2 cut(s)	762, 1144
BseGI	GGATG	5 cut(s)	133, 910, 928, 1011, 1073
BseJI	GATNNNNATC	2 cut(s)	1072, 1139
BseLI	CCNNNNNNNGG	4 cut(s)	56, 113, 615, 731
BseMII	CTCAG	2 cut(s)	519, 626
BseNI	ACTGG	4 cut(s)	462, 483, 885, 1114
BseRI	GAGGAG	1 cut(s)	212
BseSI	GKGCMC	1 cut(s)	51
BseXI	GCAGC	1 cut(s)	356
BsgI	GTGCAG	1 cut(s)	355
Bsh1236I	CGCG	1 cut(s)	212
BshFI	GGCC	2 cut(s)	49, 73
BshTI	ACCGGT	1 cut(s)	893
BsiHKAI	GWGCWC	1 cut(s)	11
BsiHKCI	CYCGRG	1 cut(s)	1130
BsiSI	CCGG	3 cut(s)	70, 491, 894
BslFI	GGGAC	2 cut(s)	233, 933
BslI	CCNNNNNNNGG	4 cut(s)	56, 113, 615, 731
BsmAI	GTCTC	3 cut(s)	524, 961, 1098
BsmFI	GGGAC	2 cut(s)	233, 933
BsmI	GAATGC	1 cut(s)	685
BsnI	GGCC	2 cut(s)	49, 73
Bso31I	GGTCTC	1 cut(s)	1098
BsoBI	CYCGRG	1 cut(s)	1130
Bsp119I	TTCGAA	1 cut(s)	654
Bsp120I	GGGCCC	1 cut(s)	47
Bsp1286I	GDGCHC	2 cut(s)	11, 51
BspACI	CCGC	4 cut(s)	98, 144, 380, 731
BspANI	GGCC	2 cut(s)	49, 73
BspCNI	CTCAG	2 cut(s)	520, 625
BspFNI	CGCG	1 cut(s)	212
BspLI	GGNNCC	2 cut(s)	49, 949
BspPI	GGATC	4 cut(s)	292, 482, 1135, 1142
BspT104I	TTCGAA	1 cut(s)	654
BspTNI	GGTCTC	1 cut(s)	1098
BsrFI	RCCGGY	1 cut(s)	893
BsrI	ACTGG	4 cut(s)	462, 483, 885, 1114
BssAI	RCCGGY	1 cut(s)	893
BssECI	CCNNGG	2 cut(s)	762, 1144
BssNI	GRCGYC	1 cut(s)	640
BssSI	CACGAG	1 cut(s)	1095
BssT1I	CCWWGG	2 cut(s)	762, 1144
Bst2BI	CACGAG	1 cut(s)	1095
Bst2UI	CCWGG	2 cut(s)	792, 1103
Bst4CI	ACNGT	2 cut(s)	507, 1162
BstACI	GRCGYC	1 cut(s)	640
BstBI	TTCGAA	1 cut(s)	654
BstC8I	GCNNGC	1 cut(s)	976
BstDEI	CTNAG	3 cut(s)	528, 612, 939
BstEII	GGTNACC	1 cut(s)	493
BstF5I	GGATG	5 cut(s)	133, 910, 928, 1011, 1073
BstFNI	CGCG	1 cut(s)	212
BstMAI	GTCTC	3 cut(s)	524, 961, 1098
BstMWI	GCNNNNNNNGC	1 cut(s)	458
BstNI	CCWGG	2 cut(s)	792, 1103
BstNSI	RCATGY	1 cut(s)	277
BstPAI	GACNNNNGTC	2 cut(s)	536, 643
BstPI	GGTNACC	1 cut(s)	493
BstSCI	CCNGG	2 cut(s)	790, 1101
BstSLI	GKGCMC	1 cut(s)	51
BstUI	CGCG	1 cut(s)	212
BstV1I	GCAGC	1 cut(s)	356
BstV2I	GAAGAC	2 cut(s)	276, 333
BstX2I	RGATCY	1 cut(s)	313
BstXI	CCANNNNNNTGG	1 cut(s)	1109
BstYI	RGATCY	1 cut(s)	313
BsuRI	GGCC	2 cut(s)	49, 73
BtsCI	GGATG	5 cut(s)	133, 910, 928, 1011, 1073
BtsI	GCAGTG	1 cut(s)	1161
BtsIMutI	CAGTG	3 cut(s)	80, 620, 1161
Cac8I	GCNNGC	1 cut(s)	976
Cfr10I	RCCGGY	1 cut(s)	893
Cfr13I	GGNCC	3 cut(s)	47, 48, 947
Csp6I	GTAC	2 cut(s)	873, 926
CspAI	ACCGGT	1 cut(s)	893
CspCI	CAANNNNNGTGG	4 cut(s)	676, 711, 837, 872
CviAII	CATG	5 cut(s)	37, 274, 281, 322, 1055
CviQI	GTAC	2 cut(s)	873, 926
DdeI	CTNAG	3 cut(s)	528, 612, 939
DraIII	CACNNNGTG	1 cut(s)	615
DriI	GACNNNNNGTC	1 cut(s)	334
EaeI	YGGCCR	1 cut(s)	71
Eam1105I	GACNNNNNGTC	1 cut(s)	334
Ecl136II	GAGCTC	1 cut(s)	9
Eco130I	CCWWGG	2 cut(s)	762, 1144
Eco24I	GRGCYC	2 cut(s)	11, 51
Eco31I	GGTCTC	1 cut(s)	1098
Eco32I	GATATC	1 cut(s)	715
Eco47I	GGWCC	1 cut(s)	947
Eco53kI	GAGCTC	1 cut(s)	9
Eco88I	CYCGRG	1 cut(s)	1130
Eco91I	GGTNACC	1 cut(s)	493
EcoICRI	GAGCTC	1 cut(s)	9
EcoO65I	GGTNACC	1 cut(s)	493
EcoRI	GAATTC	1 cut(s)	206
EcoRII	CCWGG	2 cut(s)	790, 1101
EcoRV	GATATC	1 cut(s)	715
EcoT14I	CCWWGG	2 cut(s)	762, 1144
EcoT38I	GRGCYC	2 cut(s)	11, 51
ErhI	CCWWGG	2 cut(s)	762, 1144
FaeI	CATG	5 cut(s)	40, 277, 284, 325, 1058
FaqI	GGGAC	2 cut(s)	233, 933
FatI	CATG	5 cut(s)	36, 273, 280, 321, 1054
FauI	CCCGC	1 cut(s)	724
FauNDI	CATATG	1 cut(s)	754
FbaI	TGATCA	3 cut(s)	33, 318, 438
FblI	GTMKAC	1 cut(s)	564
Fnu4HI	GCNGC	2 cut(s)	98, 370
FokI	GGATG	5 cut(s)	120, 897, 915, 998, 1080
FriOI	GRGCYC	2 cut(s)	11, 51
Fsp4HI	GCNGC	2 cut(s)	98, 370
FspBI	CTAG	2 cut(s)	63, 704
GluI	GCNGC	2 cut(s)	98, 370
HaeIII	GGCC	2 cut(s)	49, 73
HapII	CCGG	3 cut(s)	70, 491, 894
Hin1I	GRCGYC	1 cut(s)	640
Hin1II	CATG	5 cut(s)	40, 277, 284, 325, 1058
HinfI	GANTC	6 cut(s)	66, 157, 455, 656, 837, 1210
HpaII	CCGG	3 cut(s)	70, 491, 894
HphI	GGTGA	5 cut(s)	487, 832, 871, 908, 1160
Hpy166II	GTNNAC	2 cut(s)	296, 565
Hpy188I	TCNGA	5 cut(s)	13, 138, 162, 311, 318
Hpy188III	TCNNGA	6 cut(s)	386, 710, 1084, 1132, 1138, 1229
Hpy8I	GTNNAC	2 cut(s)	296, 565
Hpy99I	CGWCG	1 cut(s)	340
HpyAV	CCTTC	2 cut(s)	613, 671
HpyCH4III	ACNGT	2 cut(s)	507, 1162
HpyCH4IV	ACGT	2 cut(s)	640, 1218
HpyCH4V	TGCA	5 cut(s)	372, 511, 827, 968, 1058
HpyF10VI	GCNNNNNNNGC	1 cut(s)	458
HpyF3I	CTNAG	3 cut(s)	528, 612, 939
HpySE526I	ACGT	2 cut(s)	640, 1218
Hsp92I	GRCGYC	1 cut(s)	640
Hsp92II	CATG	5 cut(s)	40, 277, 284, 325, 1058
Ksp22I	TGATCA	3 cut(s)	33, 318, 438
LmnI	GCTCC	4 cut(s)	6, 199, 688, 983
Lsp1109I	GCAGC	1 cut(s)	356
MaeI	CTAG	2 cut(s)	63, 704
MaeII	ACGT	2 cut(s)	640, 1218
MaeIII	GTNAC	3 cut(s)	78, 493, 1156
MboII	GAAGA	5 cut(s)	281, 338, 1068, 1178, 1244
MfeI	CAATTG	1 cut(s)	1059
MflI	RGATCY	1 cut(s)	313
MhlI	GDGCHC	2 cut(s)	11, 51
MluCI	AATT	8 cut(s)	174, 206, 304, 355, 553, 830, 1059, 1120
MlyI	GAGTC	1 cut(s)	1204
MmeI	TCCRAC	1 cut(s)	1045
MnlI	CCTC	8 cut(s)	176, 190, 191, 235, 258, 391, 735, 987
MroXI	GAANNNNTTC	2 cut(s)	660, 1239
MseI	TTAA	4 cut(s)	243, 588, 635, 803
MslI	CAYNNNNRTG	2 cut(s)	805, 933
MspA1I	CMGCKG	1 cut(s)	290
MspI	CCGG	3 cut(s)	70, 491, 894
MspR9I	CCNGG	2 cut(s)	792, 1103
MunI	CAATTG	1 cut(s)	1059
Mva1269I	GAATGC	1 cut(s)	685
MvaI	CCWGG	2 cut(s)	792, 1103
MvnI	CGCG	1 cut(s)	212
MwoI	GCNNNNNNNGC	1 cut(s)	458
NdeI	CATATG	1 cut(s)	754
NlaIII	CATG	5 cut(s)	40, 277, 284, 325, 1058
NlaIV	GGNNCC	2 cut(s)	49, 949
NmuCI	GTSAC	3 cut(s)	78, 493, 1156
NspI	RCATGY	1 cut(s)	277
NspV	TTCGAA	1 cut(s)	654
PctI	GAATGC	1 cut(s)	685
PdmI	GAANNNNTTC	2 cut(s)	660, 1239
PfeI	GAWTC	5 cut(s)	66, 157, 455, 656, 837
PflFI	GACNNNGTC	1 cut(s)	1022
PflMI	CCANNNNNTGG	1 cut(s)	615
PfoI	TCCNGGA	1 cut(s)	790
PinAI	ACCGGT	1 cut(s)	893
PkrI	GCNGC	2 cut(s)	99, 371
PleI	GAGTC	1 cut(s)	1204
PpsI	GAGTC	1 cut(s)	1204
PshAI	GACNNNNGTC	2 cut(s)	536, 643
PsiI	TTATAA	2 cut(s)	303, 1184
Psp124BI	GAGCTC	1 cut(s)	11
Psp6I	CCWGG	2 cut(s)	790, 1101
PspEI	GGTNACC	1 cut(s)	493
PspGI	CCWGG	2 cut(s)	790, 1101
PspN4I	GGNNCC	2 cut(s)	49, 949
PspOMI	GGGCCC	1 cut(s)	47
PspPI	GGNCC	3 cut(s)	47, 48, 947
PsuI	RGATCY	1 cut(s)	313
PsyI	GACNNNGTC	1 cut(s)	1022
PvuII	CAGCTG	1 cut(s)	290
RsaI	GTAC	2 cut(s)	874, 927
RsaNI	GTAC	2 cut(s)	873, 926
RseI	CAYNNNNRTG	2 cut(s)	805, 933
SacI	GAGCTC	1 cut(s)	11
SaqAI	TTAA	4 cut(s)	243, 588, 635, 803
SatI	GCNGC	2 cut(s)	98, 370
Sau96I	GGNCC	3 cut(s)	47, 48, 947
SchI	GAGTC	1 cut(s)	1204
ScrFI	CCNGG	2 cut(s)	792, 1103
SduI	GDGCHC	2 cut(s)	11, 51
SfuI	TTCGAA	1 cut(s)	654
SinI	GGWCC	1 cut(s)	947
SmiMI	CAYNNNNRTG	2 cut(s)	805, 933
SmlI	CTYRAG	1 cut(s)	395
SmoI	CTYRAG	1 cut(s)	395
Sse9I	AATT	8 cut(s)	174, 206, 304, 355, 553, 830, 1059, 1120
SsiI	CCGC	4 cut(s)	98, 144, 380, 731
SspMI	CTAG	2 cut(s)	63, 704
SstI	GAGCTC	1 cut(s)	11
StyD4I	CCNGG	2 cut(s)	790, 1101
StyI	CCWWGG	2 cut(s)	762, 1144
TaaI	ACNGT	2 cut(s)	507, 1162
TaiI	ACGT	2 cut(s)	643, 1221
TaqI	TCGA	3 cut(s)	654, 835, 1026
TasI	AATT	8 cut(s)	174, 206, 304, 355, 553, 830, 1059, 1120
TatI	WGTACW	1 cut(s)	925
TauI	GCSGC	1 cut(s)	100
TfiI	GAWTC	5 cut(s)	66, 157, 455, 656, 837
Tru1I	TTAA	4 cut(s)	243, 588, 635, 803
Tru9I	TTAA	4 cut(s)	243, 588, 635, 803
TscAI	CASTG	3 cut(s)	87, 620, 1161
TseFI	GTSAC	3 cut(s)	78, 493, 1156
TseI	GCWGC	1 cut(s)	369
Tsp45I	GTSAC	3 cut(s)	78, 493, 1156
TspDTI	ATGAA	7 cut(s)	119, 516, 653, 771, 899, 1043, 1185
TspRI	CASTG	3 cut(s)	87, 620, 1161
Tth111I	GACNNNGTC	1 cut(s)	1022
Van91I	CCANNNNNTGG	1 cut(s)	615
VpaK11BI	GGWCC	1 cut(s)	947
XapI	RAATTY	2 cut(s)	174, 206
XceI	RCATGY	1 cut(s)	277
XmiI	GTMKAC	1 cut(s)	564
XmnI	GAANNNNTTC	2 cut(s)	660, 1239
XspI	CTAG	2 cut(s)	63, 704
ZraI	GACGTC	1 cut(s)	641

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.