FvH4_5g16680

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012352
Physical Location & Seq
Forward (+)
9529680 .. 9531334
1655 bp
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UTR
Exon/CDS
Intron
FvH4_5g16680.t2

Sequence Viewer

Length: 672 bp
ATGCCGGGATTAGTATACTCGTCGGATTCTGATGACATCTATGAAGCCAAACACTCGAGGCTCTTTGGACGCGAGAGGCCGATTCATCAGGTTCTTGGTGGTGGCAAAGTTGCGGATGTGTTGCTGTGGAGGAACAGAAATGTATCGGCTGCGCTTTTGGGTGTGATGACCGTGATATGGTTTCTGTTCGAGGTAGTCGAGTACAATTTCGTCACCCTTCTCTGTCACGCCTCCATTACCACCATGCTTGTCATCTTCATTTGGCGCATGGTTACAGAAATCTTCAAATTAACACCTCCTCGAATACCAAAAGTTATATTAGATGAGTCTACATTCAGAAATGTAGCTTCCATGGTCCATAAAAAGCTCAACCGTTTCTTATCAAAGCTCATTGACAGTGCTTGTGGAAAAGACCTACCATTCTTCATTTTGGCAATCTTTTCTCTGTATATATTGTCTGTGATTGGAACCTATTTCAGCTTCCTGAATCTTCTTTACTTAGGGTTCCTGTGTCTGGAAACTCTACCAGTTCTGTATGAGAAATTTGAGGAAGACGTTGATCATCTAGCCGGAAAAGTCACCCGAGAACTAAAGAGATCATACAGAAAGTTTGAGGCTCAGTTCCTCAACAAGATCCCAAGAGGCCCAGTCAAAGACAGGAAAATCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

224

Amino Acids

25.8

Weight (kDa)

9.42

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 2 - 138 2.9e-40 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012352)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18260
fragaria_vesca FvH4_5g16680 FvH4_5g16680
malus_domestica MD06G1220200.v1.1 MD14G1229700.v1.1
prunus_persica Prupe.5G224500_v2.0.a1
pyrus_communis pycom06g19640 pycom14g19120
rosa_chinensis RchiOBHm_Chr7g0179441
rosa_laevigata RLG00000005356
rosa_multiflora Rmu_co8409275.1_g000001
rosa_roxburghii Rroxscaffold_3G00274020
rosa_rugosa Rorug06G0424200 Rorug06G0424200
rosa_samantha Rh7AG023800 Rh7BG023400 Rh7CG024800 Rh7DG024300
rosa_wichuraiana Rw7G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 15, 329
AccII CGCG 1 cut(s) 72
AciI CCGC 1 cut(s) 113
AclWI GGATC 1 cut(s) 628
AcsI RAATTY 1 cut(s) 542
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 177, 514
AgsI TTSAA 1 cut(s) 286
AluBI AGCT 4 cut(s) 347, 367, 388, 480
AluI AGCT 4 cut(s) 347, 367, 388, 480
AlwI GGATC 1 cut(s) 628
Ama87I CYCGRG 2 cut(s) 55, 582
AoxI GGCC 2 cut(s) 77, 643
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 542
AspLEI GCGC 2 cut(s) 154, 267
AspS9I GGNCC 2 cut(s) 355, 644
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 205, 571
AvaI CYCGRG 2 cut(s) 55, 582
AvaII GGWCC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 136
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 1 cut(s) 6
BfaI CTAG 1 cut(s) 566
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 55, 582
BmgT120I GGNCC 2 cut(s) 355, 644
BmiI GGNNCC 2 cut(s) 469, 506
BmrFI CCNGG 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 558
BpuMI CCSGG 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 514
Bse1I ACTGG 2 cut(s) 527, 647
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 177, 514
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 2 cut(s) 527, 647
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 79, 645
BsiHKCI CYCGRG 2 cut(s) 55, 582
BsiSI CCGG 2 cut(s) 5, 570
BslI CCNNNNNNNGG 2 cut(s) 177, 514
BsnI GGCC 2 cut(s) 79, 645
BsoBI CYCGRG 2 cut(s) 55, 582
Bsp143I GATC 3 cut(s) 559, 596, 633
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 1 cut(s) 113
BspANI GGCC 2 cut(s) 79, 645
BspCNI CTCAG 1 cut(s) 631
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 469, 506
BspPI GGATC 1 cut(s) 628
BsrI ACTGG 2 cut(s) 527, 647
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 3 cut(s) 559, 596, 633
BssNAI GTATAC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 16
Bst4CI ACNGT 3 cut(s) 172, 374, 398
BstDEI CTNAG 2 cut(s) 499, 618
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 121
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 2 cut(s) 154, 267
BstKTI GATC 3 cut(s) 562, 599, 636
BstMBI GATC 3 cut(s) 559, 596, 633
BstSCI CCNGG 1 cut(s) 4
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 136
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 633
BstYI RGATCY 1 cut(s) 633
BstZ17I GTATAC 1 cut(s) 16
BsuRI GGCC 2 cut(s) 79, 645
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 403
CfoI GCGC 2 cut(s) 154, 267
Cfr13I GGNCC 2 cut(s) 355, 644
CseI GACGC 1 cut(s) 78
Csp6I GTAC 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 3 cut(s) 244, 268, 352
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 499, 618
DpnI GATC 3 cut(s) 561, 598, 635
DpnII GATC 3 cut(s) 559, 596, 633
Eco130I CCWWGG 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 355
Eco88I CYCGRG 2 cut(s) 55, 582
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 3 cut(s) 247, 271, 355
FatI CATG 3 cut(s) 243, 267, 351
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 2 cut(s) 15, 329
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 566
GlaI GCGC 2 cut(s) 153, 266
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 2 cut(s) 79, 645
HapII CCGG 2 cut(s) 5, 570
HgaI GACGC 1 cut(s) 78
HhaI GCGC 2 cut(s) 154, 267
Hin1II CATG 3 cut(s) 247, 271, 355
Hin6I GCGC 2 cut(s) 152, 265
HinP1I GCGC 2 cut(s) 152, 265
HinfI GANTC 4 cut(s) 26, 82, 326, 487
HpaII CCGG 2 cut(s) 5, 570
HphI GGTGA 2 cut(s) 205, 571
Hpy166II GTNNAC 2 cut(s) 16, 330
Hpy188I TCNGA 4 cut(s) 25, 31, 338, 668
Hpy188III TCNNGA 2 cut(s) 484, 515
Hpy8I GTNNAC 2 cut(s) 16, 330
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 3 cut(s) 172, 374, 398
HpyCH4IV ACGT 1 cut(s) 555
HpyF3I CTNAG 2 cut(s) 499, 618
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 3 cut(s) 247, 271, 355
HspAI GCGC 2 cut(s) 152, 265
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 3 cut(s) 559, 596, 633
LpnPI CCDG 9 cut(s) 18, 74, 497, 500, 521, 540, 583, 643, 660
Lsp1109I GCAGC 1 cut(s) 136
MaeI CTAG 1 cut(s) 566
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 4 cut(s) 211, 224, 271, 577
MalI GATC 3 cut(s) 561, 598, 635
MboI GATC 3 cut(s) 559, 596, 633
MboII GAAGA 5 cut(s) 247, 274, 415, 482, 563
MflI RGATCY 1 cut(s) 633
MluCI AATT 3 cut(s) 205, 287, 542
MlyI GAGTC 1 cut(s) 335
MseI TTAA 1 cut(s) 290
MspI CCGG 2 cut(s) 5, 570
MspR9I CCNGG 1 cut(s) 6
MvnI CGCG 1 cut(s) 72
NciI CCSGG 1 cut(s) 6
NcoI CCATGG 1 cut(s) 351
NdeII GATC 3 cut(s) 559, 596, 633
NlaIII CATG 3 cut(s) 247, 271, 355
NlaIV GGNNCC 2 cut(s) 469, 506
NmuCI GTSAC 3 cut(s) 211, 224, 577
PaeR7I CTCGAG 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 3 cut(s) 26, 82, 487
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 469, 506
PspPI GGNCC 2 cut(s) 355, 644
PspXI VCTCGAGB 1 cut(s) 55
PsuI RGATCY 1 cut(s) 633
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 3 cut(s) 559, 596, 633
Sau96I GGNCC 2 cut(s) 355, 644
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 3 cut(s) 205, 287, 542
SsiI CCGC 1 cut(s) 113
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 4
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 172, 374, 398
TaiI ACGT 1 cut(s) 558
TaqI TCGA 4 cut(s) 56, 189, 198, 301
TasI AATT 3 cut(s) 205, 287, 542
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 3 cut(s) 26, 82, 487
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 3 cut(s) 211, 224, 577
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 3 cut(s) 211, 224, 577
TspDTI ATGAA 4 cut(s) 57, 74, 247, 415
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 355
XapI RAATTY 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 55
XmiI GTMKAC 2 cut(s) 15, 329
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.